FastQCFastQC Report
Thu 26 May 2016
SRR937463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673435
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104651.5539732862117355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76011.1286909649780603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.7862674200182647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17790.2641680340344651No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14650.21754141082658313No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.19333714463905202No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11500.17076629518810277No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.12428816441081916No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8080.11998188392346701No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.1161210807279099No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.10023239065388642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51000.040.462711
GTATCAA85800.037.296081
ATCAACG108450.029.5789853
TATCAAC108950.029.4039962
TCAACGC109050.029.2833184
CAACGCA110400.028.923085
AACGCAG112750.028.353956
AAGCGTA350.008402239527.13611692-93
GTACATG69850.025.7312471
CGCAGAG124900.025.4436118
ACGCAGA125000.025.4232547
TACCTGG5450.025.2915362
GAGTACT68950.024.89958612-13
TACATGG71300.024.865262
GCAGAGT128450.024.4445699
ATGGGAT19650.024.4232335
AGTACTT70350.023.76274712-13
ACATGGG71850.023.6129633
GTACTTT74050.023.15348614-15
CTAATAG1252.2469874E-422.8113123
Produced by FastQC (version 0.11.5)