FastQCFastQC Report
Thu 26 May 2016
SRR937463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673435
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91061.3521720730285773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73541.0920133346202678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53330.7919101323810018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22770.3381172644724435No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.2530311017395888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.20699844825410024No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12530.18606101553973287No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.1400283620542443No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.11226027753235279No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.10335073169645177No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.10142033009867323No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC550.00257732834.5496671
GTATCAA92850.033.4101071
GGTATCA59800.032.8886221
GTATAGG2053.092282E-1127.808271
CGATCCG350.00839620127.14012170-71
ATCAACG116150.026.6202163
TATCAAC117000.026.4674172
TAACGCG458.951678E-426.38720776-77
GGACCGA908.948951E-426.3862296
TCAACGC117700.026.2696534
CAACGCA118700.026.0483445
AACGCAG121550.025.3594346
GAGTACT66450.024.16576612-13
AACGCGA500.001654406523.74848676-77
GTACATG79450.023.3193951
AGTACTT67700.022.8423712-13
GTACTTT71250.022.63105214-15
ACGCAGA135250.022.6218137
CGCAGAG136050.022.4538758
TACATGG81800.022.2960412
Produced by FastQC (version 0.11.5)