##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937463_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 673435 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28295084158085 33.0 31.0 34.0 31.0 34.0 2 32.452346551634534 34.0 31.0 34.0 31.0 34.0 3 32.48026312858702 34.0 31.0 34.0 31.0 34.0 4 36.12371646855301 37.0 35.0 37.0 35.0 37.0 5 36.08034034465093 37.0 35.0 37.0 35.0 37.0 6 36.15267100759539 37.0 35.0 37.0 35.0 37.0 7 36.09774811228998 37.0 35.0 37.0 35.0 37.0 8 36.08860988811095 37.0 35.0 37.0 35.0 37.0 9 37.762345289448874 39.0 37.0 39.0 35.0 39.0 10-11 37.69721428200198 39.0 37.0 39.0 35.0 39.0 12-13 37.58965527482236 39.0 37.0 39.0 35.0 39.0 14-15 38.711701945993305 40.0 38.0 41.0 34.5 41.0 16-17 38.706177285112894 40.0 38.0 41.0 34.5 41.0 18-19 38.80551203902381 40.0 38.0 41.0 35.0 41.0 20-21 38.78131000022274 40.0 38.0 41.0 35.0 41.0 22-23 38.70383927179312 40.0 38.0 41.0 35.0 41.0 24-25 38.69962431415058 40.0 38.0 41.0 35.0 41.0 26-27 38.51210658786668 40.0 38.0 41.0 34.0 41.0 28-29 38.34405250692346 40.0 38.0 41.0 34.0 41.0 30-31 38.215071239243585 40.0 37.5 41.0 34.0 41.0 32-33 37.957383414880425 40.0 36.5 41.0 34.0 41.0 34-35 37.76408042350042 40.0 36.0 41.0 33.0 41.0 36-37 37.53812691648044 40.0 36.0 41.0 33.0 41.0 38-39 37.37216138157358 40.0 35.5 41.0 33.0 41.0 40-41 37.097010847372054 40.0 35.0 41.0 33.0 41.0 42-43 36.7640759687275 40.0 35.0 41.0 31.5 41.0 44-45 36.229071848062546 39.0 35.0 41.0 30.5 41.0 46-47 35.55161745380029 39.0 35.0 41.0 28.0 41.0 48-49 34.96799394150884 39.0 35.0 41.0 23.5 41.0 50-51 34.63298610853312 39.0 35.0 41.0 22.5 41.0 52-53 34.3654042335192 39.0 35.0 40.0 20.0 41.0 54-55 34.14307022949505 38.0 34.5 40.0 18.5 41.0 56-57 33.84456035103611 38.0 34.0 40.0 16.0 41.0 58-59 33.58921202491703 38.0 34.0 40.0 12.5 41.0 60-61 33.24950514897503 37.5 33.5 40.0 8.0 41.0 62-63 32.88489238011093 37.0 33.0 40.0 6.0 41.0 64-65 32.48759939712073 36.5 33.0 39.5 2.0 41.0 66-67 32.0594734458411 36.0 32.0 39.0 2.0 40.5 68-69 31.625433783512886 35.0 31.5 39.0 2.0 40.0 70-71 31.19913354666746 35.0 31.0 38.0 2.0 40.0 72-73 30.737416380200017 35.0 31.0 37.5 2.0 40.0 74-75 30.2622658459985 35.0 30.0 37.0 2.0 39.0 76-77 28.97128973100596 33.5 28.5 35.5 2.0 38.0 78-79 29.5489891377787 34.0 30.0 36.0 2.0 38.5 80-81 29.350034524490113 34.0 30.0 36.0 2.0 37.5 82-83 28.951906271577805 34.0 29.0 35.0 2.0 37.0 84-85 28.519543088791046 34.0 29.0 35.0 2.0 36.5 86-87 28.169062344546987 34.0 29.0 35.0 2.0 36.0 88-89 27.750464410076695 34.0 27.5 35.0 2.0 36.0 90-91 27.223171501332722 33.5 26.5 35.0 2.0 35.0 92-93 26.761827793328237 33.0 25.5 35.0 2.0 35.0 94-95 26.318271993585128 33.0 25.0 35.0 2.0 35.0 96-97 25.740142701225807 33.0 23.5 35.0 2.0 35.0 98-99 24.906447541336583 32.0 10.5 34.5 2.0 35.0 100-101 22.267263358750288 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2859296729679244 1101 2 0.24836599235935353 1101 3 0.15338079784365988 1101 4 0.1886272163428231 1101 5 0.10847916550527259 1101 6 0.17536146633805316 1101 7 0.19785484368188833 1101 8 0.08915431443462296 1101 9 0.24858451500788448 1101 10-11 0.25313004900207403 1101 12-13 0.1744791383282447 1101 14-15 0.24466957240066733 1101 16-17 0.2129304295675567 1101 18-19 0.3078004957567728 1101 20-21 0.3972069951169743 1101 22-23 0.4011383216238613 1101 24-25 0.1831543481724509 1101 26-27 0.3608089677517299 1101 28-29 0.17475077320688825 1101 30-31 0.2769845904249095 1101 32-33 0.5260292368124126 1101 34-35 0.31754590341212463 1101 36-37 0.43252692916750846 1101 38-39 0.17515518256801244 1101 40-41 0.11536331254211518 1101 42-43 0.19612464593585344 1101 44-45 0.22646423934019566 1101 46-47 -0.07921805262857617 1101 48-49 -0.09377878924325245 1101 50-51 -0.10882859219839958 1101 52-53 -0.1842573793315765 1101 54-55 -0.14639876984785616 1101 56-57 0.18097185647157943 1101 58-59 0.21131785933020808 1101 60-61 0.03251277386260654 1101 62-63 0.222406174820712 1101 64-65 0.2225235673995627 1101 66-67 0.11341776724530206 1101 68-69 -0.08172903561949241 1101 70-71 -0.29402576982217 1101 72-73 -0.1853385511750325 1101 74-75 -0.026035427540904266 1101 76-77 -0.23844466325995128 1101 78-79 -0.15542614140739985 1101 80-81 0.18474087477766687 1101 82-83 0.09210531667124755 1101 84-85 0.2574414627072592 1101 86-87 0.36268867698840523 1101 88-89 0.5401233293844143 1101 90-91 0.31244823019854806 1101 92-93 0.41530170640629294 1101 94-95 0.3725268060196427 1101 96-97 0.8237832232617457 1101 98-99 0.9847360935702199 1101 100-101 1.276302828162848 1102 1 0.26277224184331516 1102 2 0.27148865256635446 1102 3 0.2170270308010842 1102 4 0.25164757278336225 1102 5 0.20003099155795923 1102 6 0.16760948342989224 1102 7 0.18476552492143128 1102 8 0.20015467373410445 1102 9 0.34976981695466947 1102 10-11 0.21339365119187192 1102 12-13 0.22539148857183733 1102 14-15 0.3032662954348595 1102 16-17 0.4081227706934385 1102 18-19 0.37232759196017895 1102 20-21 0.14952022699650058 1102 22-23 0.1227565601291758 1102 24-25 0.08141464326163828 1102 26-27 0.1289767722000832 1102 28-29 0.1622757406574422 1102 30-31 0.1288575838532111 1102 32-33 0.2705180463720964 1102 34-35 0.30164883305509704 1102 36-37 0.29274880625617783 1102 38-39 0.2134534165917401 1102 40-41 0.23205547788169412 1102 42-43 0.12136620722731806 1102 44-45 0.007642162125577556 1102 46-47 0.221946386767641 1102 48-49 0.20390179738075886 1102 50-51 0.4364914354637932 1102 52-53 0.55591597300986 1102 54-55 0.6572894586511637 1102 56-57 0.45065412755338485 1102 58-59 0.4984638237788772 1102 60-61 0.6563155918523123 1102 62-63 0.7450005102518844 1102 64-65 0.5829733871703127 1102 66-67 0.4416475025613522 1102 68-69 0.4080347925812653 1102 70-71 0.3422685003169512 1102 72-73 0.4465369148518512 1102 74-75 0.3995837845501029 1102 76-77 0.38731305298617613 1102 78-79 0.4872215480651221 1102 80-81 0.3187663425430962 1102 82-83 0.5687209087233889 1102 84-85 0.7344491934412751 1102 86-87 0.8281611558071198 1102 88-89 0.7383320476124915 1102 90-91 0.9022991788650856 1102 92-93 1.0117658904294409 1102 94-95 1.1484359689291175 1102 96-97 0.947311737345899 1102 98-99 1.197383435333574 1102 100-101 1.9325343561128712 1103 1 0.18594942093449873 1103 2 0.22887104660454582 1103 3 0.12944255960913154 1103 4 0.20587583089954364 1103 5 0.12030326049298878 1103 6 0.12441171257051309 1103 7 0.11719674269572522 1103 8 0.11229646276653682 1103 9 0.29514034621214336 1103 10-11 0.14262783352707942 1103 12-13 0.18064729226365017 1103 14-15 0.2750629272099161 1103 16-17 0.23970743799746685 1103 18-19 0.3154836890029671 1103 20-21 0.3167834012339057 1103 22-23 0.24823969040478744 1103 24-25 0.22335688825464928 1103 26-27 0.021113086437004824 1103 28-29 0.09002273973408847 1103 30-31 -0.16830737091844128 1103 32-33 -0.2824710285508871 1103 34-35 -0.29642763431266417 1103 36-37 -0.24612428471531445 1103 38-39 -0.4049043081813011 1103 40-41 -0.3275033516560484 1103 42-43 -0.2532711897116968 1103 44-45 -0.45728441841519896 1103 46-47 -0.6242588445220392 1103 48-49 -0.8671091603815952 1103 50-51 -0.9876254473347288 1103 52-53 -0.8870011124378081 1103 54-55 -0.9976533828494212 1103 56-57 -0.9388551019712921 1103 58-59 -1.1690526618926853 1103 60-61 -0.9798883320235134 1103 62-63 -0.9304585145808026 1103 64-65 -0.9798498913333553 1103 66-67 -0.7531878041497677 1103 68-69 -0.8854959602439756 1103 70-71 -0.8023662000809928 1103 72-73 -0.5411938232649831 1103 74-75 -0.6338778387676207 1103 76-77 -0.5697384019203007 1103 78-79 -0.6065098110732912 1103 80-81 -0.44383998697422555 1103 82-83 -0.4292934564863309 1103 84-85 -0.5523177355252962 1103 86-87 -0.6987490984031623 1103 88-89 -0.747829793879923 1103 90-91 -0.7238417440056679 1103 92-93 -0.9548276427747844 1103 94-95 -0.6649511317989472 1103 96-97 -0.4271949182077499 1103 98-99 -0.0716721215838767 1103 100-101 0.4915926912847546 1104 1 0.13441881072617434 1104 2 0.20568137388249852 1104 3 0.1853041440671177 1104 4 0.19729172018626429 1104 5 0.21002428188440803 1104 6 0.14420246863487307 1104 7 0.14591688990893203 1104 8 0.18553583192121437 1104 9 0.15013453831579682 1104 10-11 0.17716984796897606 1104 12-13 0.15490992892883781 1104 14-15 0.2830323189974351 1104 16-17 0.3169724177796027 1104 18-19 0.27422766655374886 1104 20-21 0.26439058785698677 1104 22-23 0.31025445164236487 1104 24-25 0.25675827975476295 1104 26-27 0.3144158434913038 1104 28-29 0.16117745331099087 1104 30-31 0.3476613502666055 1104 32-33 0.24942849284652624 1104 34-35 0.06706983695720936 1104 36-37 0.3599326925440849 1104 38-39 0.2914824218732619 1104 40-41 0.4252519063861868 1104 42-43 0.43442964175552845 1104 44-45 0.7292328795358713 1104 46-47 0.7830812587028362 1104 48-49 1.0217444686554913 1104 50-51 0.8996594509372713 1104 52-53 0.812606904454988 1104 54-55 0.9387930627579308 1104 56-57 0.8950809752437934 1104 58-59 0.7080773990602864 1104 60-61 0.9058507876510831 1104 62-63 0.8089794966038895 1104 64-65 0.8828442540676349 1104 66-67 0.9030256470179978 1104 68-69 0.9132394751087496 1104 70-71 0.82949200741459 1104 72-73 0.9969915983164448 1104 74-75 0.9059092449499069 1104 76-77 1.0561522419778164 1104 78-79 1.1018988652452606 1104 80-81 1.1806435892529201 1104 82-83 0.8022709632629166 1104 84-85 0.9400641406447647 1104 86-87 0.94132598512277 1104 88-89 0.9402173159432081 1104 90-91 0.8851660836814297 1104 92-93 1.1711168888213699 1104 94-95 1.2254472779152081 1104 96-97 1.2059555961203898 1104 98-99 1.218346479651153 1104 100-101 2.061710565634609 1105 1 0.014723891301891001 1105 2 0.051321545517865275 1105 3 -0.008341969074884048 1105 4 -0.018967370631024494 1105 5 -0.0415864062177036 1105 6 -0.0680411478258165 1105 7 -0.08681537691539631 1105 8 -0.005053232842307409 1105 9 -0.0034871918817813707 1105 10-11 0.1808658469995308 1105 12-13 0.0924368961348776 1105 14-15 0.09029689414114728 1105 16-17 0.1813367812250135 1105 18-19 0.17056067735165215 1105 20-21 0.12947377078599231 1105 22-23 0.1637346212213444 1105 24-25 0.014464703962914882 1105 26-27 0.09346652439604242 1105 28-29 0.11908198894258959 1105 30-31 0.04321146290919842 1105 32-33 -0.006421669553439813 1105 34-35 -0.026294991012676405 1105 36-37 -0.0188695687536935 1105 38-39 -0.004484844758273709 1105 40-41 -0.025061418121794077 1105 42-43 -0.14024220751088023 1105 44-45 -0.19545999267583625 1105 46-47 -0.07953976093754989 1105 48-49 -0.05890560855105065 1105 50-51 0.03958964374246676 1105 52-53 0.17140156444327914 1105 54-55 0.011744519032966139 1105 56-57 0.18211486716932512 1105 58-59 0.17302416235294515 1105 60-61 -0.040699521450477505 1105 62-63 0.13251803469213996 1105 64-65 0.15397415016470006 1105 66-67 0.045476757194755635 1105 68-69 -0.06892315252072123 1105 70-71 0.01210428738513869 1105 72-73 -0.1285456802311593 1105 74-75 0.16010878483199775 1105 76-77 -0.010916461741867778 1105 78-79 -0.006896253759297366 1105 80-81 0.11698721250704835 1105 82-83 0.15786627982807389 1105 84-85 0.07914882547501279 1105 86-87 0.3861412127122179 1105 88-89 0.3914486729972779 1105 90-91 0.46575832483756585 1105 92-93 0.46562540173716727 1105 94-95 0.4543798080312662 1105 96-97 0.4252083910704769 1105 98-99 0.5235766946727338 1105 100-101 0.7948871602112426 1106 1 0.06161687173917585 1106 2 0.1648097718201882 1106 3 0.1562386090471648 1106 4 0.1435135217998038 1106 5 0.0314109487794596 1106 6 0.11705108376651197 1106 7 0.09763063239364556 1106 8 -0.011191271074622477 1106 9 0.04455224270592595 1106 10-11 -0.10727414922188672 1106 12-13 -0.17662558776323323 1106 14-15 0.13831485955000744 1106 16-17 0.16183739953736875 1106 18-19 0.10130432676854895 1106 20-21 0.0991174253554874 1106 22-23 0.08920436685450994 1106 24-25 0.10206754132959617 1106 26-27 0.1646196769302648 1106 28-29 0.10294468066235396 1106 30-31 0.16956509463549452 1106 32-33 0.09113105874981642 1106 34-35 -0.2400163725972675 1106 36-37 0.3567938288726751 1106 38-39 0.29133789711350033 1106 40-41 0.09356008884280698 1106 42-43 -0.025259726360815193 1106 44-45 0.044220846511421996 1106 46-47 0.010983958211241429 1106 48-49 0.23259538761885068 1106 50-51 0.2744656807038055 1106 52-53 0.24471773674631692 1106 54-55 0.21836496971396713 1106 56-57 0.40236395258231283 1106 58-59 0.3957647459710998 1106 60-61 0.4968232358791056 1106 62-63 0.5296484642474724 1106 64-65 0.5941041245734482 1106 66-67 0.8480826861231847 1106 68-69 0.9738702882157533 1106 70-71 0.9292719015582271 1106 72-73 0.8951369709547983 1106 74-75 1.0253091781473564 1106 76-77 0.9078366512890632 1106 78-79 0.995115408298652 1106 80-81 0.6696639897256738 1106 82-83 0.7242575684940675 1106 84-85 0.9682122110786402 1106 86-87 1.1360918336008723 1106 88-89 1.1448483174676767 1106 90-91 0.9899315467601717 1106 92-93 1.0434640565643 1106 94-95 1.0441896769833967 1106 96-97 1.3978512899248798 1106 98-99 1.6349995859699895 1106 100-101 2.4106607806018054 1107 1 0.02530657649888468 1107 2 0.03328093129965737 1107 3 0.10350413002289116 1107 4 0.03883681258397331 1107 5 0.08284054737338664 1107 6 0.0032402115382694774 1107 7 0.012946555485591205 1107 8 0.07188389713955701 1107 9 0.05357100812925353 1107 10-11 0.09990668767693478 1107 12-13 0.0671762301926293 1107 14-15 0.09215138554997537 1107 16-17 0.19141136051523233 1107 18-19 0.1890475493793744 1107 20-21 0.25718438258736853 1107 22-23 0.23408825912642328 1107 24-25 0.026162557929090724 1107 26-27 0.2191627912448908 1107 28-29 0.08920460041664313 1107 30-31 0.05602829168131507 1107 32-33 0.12418248169085899 1107 34-35 0.12453348803794739 1107 36-37 0.14982825286117674 1107 38-39 0.264606711358887 1107 40-41 0.08520707874107813 1107 42-43 0.17085181872413102 1107 44-45 0.08900509740354323 1107 46-47 0.2602593945028957 1107 48-49 0.09368221098952034 1107 50-51 0.11308326197033836 1107 52-53 -0.026647461116162674 1107 54-55 -0.2443094581802967 1107 56-57 -0.19528127084517877 1107 58-59 0.03087535012186038 1107 60-61 -0.05683997181880329 1107 62-63 0.0345373274924512 1107 64-65 -0.15545092895328594 1107 66-67 -0.21643566209347753 1107 68-69 -0.14931330934846798 1107 70-71 -0.09391857547764815 1107 72-73 0.014140734691551415 1107 74-75 -0.05834630678882391 1107 76-77 -0.09684249902961284 1107 78-79 -0.06761871565611344 1107 80-81 -0.011350443849696745 1107 82-83 -0.08418243363635014 1107 84-85 -0.24216536095608632 1107 86-87 0.05251069134227748 1107 88-89 0.023313573916624364 1107 90-91 0.15045957949758204 1107 92-93 0.6012516184795444 1107 94-95 0.4819726229950767 1107 96-97 0.5364759725815098 1107 98-99 0.6326672231599666 1107 100-101 1.1810162495598568 1108 1 0.1914348208218115 1108 2 0.1884017217561862 1108 3 -0.02726421179387728 1108 4 0.02921612455901368 1108 5 0.046540375603527195 1108 6 0.13874666997994467 1108 7 0.10438642815527288 1108 8 0.11236275158692877 1108 9 -0.06048260297747987 1108 10-11 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86-87 -0.026697119737985275 2316 88-89 0.1445322189449456 2316 90-91 -0.004948371518537442 2316 92-93 -0.09229043667797754 2316 94-95 -0.025016254953623474 2316 96-97 0.07777921231823015 2316 98-99 0.47408222162447444 2316 100-101 0.6903115363808752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 7.0 5 18.0 6 30.0 7 85.0 8 219.0 9 387.0 10 832.0 11 1391.0 12 2319.0 13 3478.0 14 4218.0 15 6498.0 16 9658.0 17 14595.0 18 12502.0 19 5824.0 20 4381.0 21 4280.0 22 4267.0 23 4544.0 24 5047.0 25 5755.0 26 6698.0 27 7862.0 28 9656.0 29 11906.0 30 14471.0 31 18182.0 32 23393.0 33 30486.0 34 42459.0 35 62630.0 36 92120.0 37 137241.0 38 115108.0 39 10884.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.19218484923212 20.601823654022706 15.80559574777837 18.400395748966805 2 15.303184420174182 22.71488710862964 40.598127510450155 21.383800960746026 3 19.15210822128342 29.57880122060778 30.913896664117544 20.355193893991252 4 11.799505520206107 18.45478776719357 38.24303756116032 31.502669151440006 5 11.642670040449758 40.904298603562665 32.454100512601194 14.998930843386377 6 27.417642385679393 38.80953618389303 18.000549421993213 15.77227200843437 7 25.77754348972061 37.23150712392436 20.307973301060976 16.68297608529405 8 27.53569386800508 33.655957887546684 19.45339936296747 19.354948881480766 9 28.884599107560494 16.197257344806847 20.574665706415615 34.343477841217044 10-11 24.36901853927996 29.215514489148912 25.708717248138274 20.70674972343285 12-13 26.712221968119703 27.234353142439645 27.475890786077688 18.577534103362964 14-15 23.425794620119238 26.894503552681403 28.63082554366791 21.04887628353145 16-17 19.445306525499863 30.78849480647724 28.792162569513017 20.97403609850988 18-19 19.5136872897904 27.318003964747895 34.51498659855814 18.653322146903562 20-21 21.501735879091342 25.754194174930284 34.581488312143435 18.162581633834943 22-23 20.61540083542338 26.409758386531063 34.42572438750221 18.549116390543354 24-25 19.842078300058656 26.538789935183054 34.93989768871531 18.679234076042974 26-27 19.6731328799782 26.74995749331587 34.96737194350955 18.609537683196383 28-29 19.26988395688533 26.981713315815618 34.94992304134087 18.79847968595818 30-31 19.518542277342693 26.79973093131621 34.90111874363338 18.78060804770772 32-33 19.38126082140195 26.989504539420345 34.54087557206794 19.08835906710977 34-35 19.495719705688003 26.970680169578355 34.6711263893323 18.862473735401338 36-37 19.794263737405988 26.836814243393942 34.36753361497398 19.001388404226095 38-39 19.68593850928449 27.092295470238405 34.28252169845642 18.939244322020684 40-41 19.648666909204305 27.39499729001314 34.048720366479316 18.90761543430324 42-43 19.567257714811845 27.58464759868128 33.47381864623244 19.374276040274435 44-45 19.779191755700253 27.748186536191316 32.99954709808667 19.473074610021754 46-47 20.149903108689035 28.35032334226763 31.95497709504258 19.544796454000757 48-49 20.18724895498452 28.6678001588869 31.23887234848204 19.906078537646543 50-51 20.266098435632244 28.989954487070023 30.915827065715323 19.82812001158241 52-53 20.342794776036293 29.45570099564175 30.1977176713417 20.00378655698026 54-55 20.00252437132017 29.45028102192491 30.242562385382403 20.304632221372515 56-57 20.448966863914112 29.63715874583293 29.738282091070406 20.175592299182547 58-59 20.37345846295485 29.214623534565327 30.106691811384916 20.305226191094906 60-61 20.236251457081973 29.31396497063562 30.63265200056427 19.817131571718132 62-63 20.359128943402112 29.244470513115594 30.57518542992271 19.82121511355959 64-65 20.163490166088785 29.340767854358624 30.485644494271902 20.01009748528069 66-67 20.27448825796105 29.47032750005568 30.10171731495987 20.153466927023395 68-69 20.457059701381723 29.381529026557875 30.101420330098673 20.059990941961733 70-71 20.300474433315763 29.782829820249912 29.758996785138876 20.15769896129545 72-73 20.29673308500394 29.651925542759606 29.62155877306371 20.429782599172746 74-75 20.164603859318273 30.198905610786493 29.26362603666278 20.372864493232456 76-77 19.894124896983374 29.808890241819924 29.889818616496022 20.407166244700676 78-79 20.030737933133857 29.726254204191942 29.589492675610863 20.65351518706334 80-81 19.82990192074959 30.106246334093118 29.432461930253105 20.631389814904185 82-83 19.84243421394556 30.101064248410754 29.27915620410642 20.777345333537266 84-85 19.761119694817676 29.789578332530237 29.166279966767323 21.28302200588477 86-87 19.53113515038571 29.696332979426376 29.184999294660958 21.587532575526964 88-89 19.677622933170984 29.37558932933394 29.270456688470304 21.676331049024775 90-91 19.551346546960403 29.327242580421924 29.37832886947082 21.743082003146856 92-93 19.486447677328055 29.346462200060287 29.55754669771185 21.609543424899805 94-95 19.225389235783705 29.308025273411687 29.468100113596705 21.998485377207896 96-97 19.155300808541284 29.345222627276573 29.32124109973494 22.178235464447198 98-99 19.178536824403338 29.422844902792782 29.010628385175945 22.387989887627935 100-101 19.008670715525238 29.449691360545682 29.400237763390525 22.141400160538556 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1434.0 1 987.0 2 634.0 3 767.5 4 689.0 5 646.0 6 1086.5 7 2115.0 8 3198.5 9 3633.0 10 3319.5 11 2867.0 12 2732.0 13 2767.0 14 2851.5 15 2853.5 16 2825.0 17 2925.5 18 3022.0 19 3083.5 20 3258.5 21 3591.0 22 3994.0 23 4447.5 24 5038.0 25 5861.5 26 6820.5 27 7729.5 28 8628.5 29 9838.0 30 11230.0 31 12329.0 32 13836.5 33 15491.0 34 16981.5 35 18050.5 36 19621.0 37 21528.5 38 21886.5 39 21475.5 40 21193.5 41 21159.5 42 20872.5 43 21003.0 44 21964.0 45 22606.5 46 22308.5 47 22483.0 48 25081.5 49 24522.0 50 21237.5 51 18585.5 52 15928.5 53 14677.5 54 13778.0 55 12804.5 56 11771.0 57 10930.0 58 10326.0 59 9624.5 60 8450.5 61 7237.0 62 5916.5 63 4020.0 64 2510.5 65 1799.0 66 1285.5 67 977.5 68 714.5 69 467.5 70 345.5 71 283.0 72 247.5 73 266.5 74 227.5 75 168.5 76 116.0 77 65.5 78 47.5 79 34.0 80 15.0 81 11.5 82 8.5 83 4.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041726372998136425 2 0.0 3 0.0 4 0.0 5 0.0010394470141884516 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00608818965453236 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.4849243059835026E-4 22-23 5.93969722393401E-4 24-25 0.0 26-27 0.001707662951881028 28-29 0.0031925872578645303 30-31 1.4849243059835026E-4 32-33 1.4849243059835026E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.008167083682909264 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.2273864589752537E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.969848611967005E-4 84-85 2.2273864589752537E-4 86-87 0.0 88-89 0.0 90-91 0.00957776177359359 92-93 0.001187939444786802 94-95 0.0 96-97 0.0 98-99 3.7123107649587563E-4 100-101 0.011359670940773794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 673435.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.76844332739882 #Duplication Level Percentage of deduplicated Percentage of total 1 78.8481381066611 44.76086059578906 2 12.133187856613334 13.775643744376756 3 3.883125054866679 6.613168938312046 4 1.6885984179141778 3.834364143603853 5 0.8888307798954368 2.52287698780709 6 0.5507242533534924 1.8758255139313063 7 0.3750038807056675 1.4901870584576018 8 0.2545704156656964 1.156125297164036 9 0.20179888765983195 1.0310227845884363 >10 1.0600651340356506 10.919753890796448 >50 0.07089206503509231 2.77521162175921 >100 0.04111206447161411 4.1874976011305005 >500 0.0021083109985317347 0.8054836400288592 >1k 0.0010541554992658673 0.9915946445228971 >5k 7.906166244494005E-4 3.26038353773197 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9106 1.3521720730285773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7354 1.0920133346202678 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5333 0.7919101323810018 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2277 0.3381172644724435 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1704 0.2530311017395888 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1394 0.20699844825410024 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1253 0.18606101553973287 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 943 0.1400283620542443 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 756 0.11226027753235279 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 696 0.10335073169645177 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 683 0.10142033009867323 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4849243059835026E-4 0.0 12-13 0.0 0.0 0.0 1.4849243059835026E-4 0.0 14-15 0.0 0.0 0.0 1.4849243059835026E-4 0.0 16-17 0.0 0.0 0.0 1.4849243059835026E-4 0.0 18-19 0.0 0.0 0.0 1.4849243059835026E-4 0.0 20-21 0.0 0.0 0.0 1.4849243059835026E-4 0.0 22-23 0.0 0.0 0.0 1.4849243059835026E-4 0.0 24-25 0.0 0.0 0.0 3.7123107649587563E-4 0.0 26-27 0.0 0.0 0.0 6.682159376925761E-4 0.0 28-29 0.0 0.0 0.0 0.002375878889573604 0.0 30-31 0.0 0.0 0.0 0.005568466147438134 0.0 32-33 0.0 0.0 0.0 0.008315576113507615 0.0 34-35 0.0 0.0 0.0 0.010245977711286167 0.0 36-37 0.0 0.0 0.0 0.01247336417026142 0.0 38-39 0.0 0.0 0.0 0.014403765768039974 0.0 40-41 0.0 0.0 0.0 0.021754141082658312 0.0 42-43 0.0 0.0 0.0 0.03088642556445685 0.0 44-45 0.0 0.0 0.0 0.05174961206352506 0.0 46-47 0.0 0.0 0.0 0.1011975914527757 0.0 48-49 0.0 0.0 0.0 0.1423299947285187 0.0 50-51 0.0 0.0 0.0 0.19207495897896604 0.0 52-53 0.0 0.0 0.0 0.2480566053145441 0.0 54-55 0.0 0.0 0.0 0.3038155130042246 0.0 56-57 0.0 0.0 0.0 0.3718992924335682 0.0 58-59 0.0 0.0 0.0 0.4806699978468597 0.0 60-61 0.0 0.0 0.0 0.6270092882015339 0.0 62-63 0.0 0.0 0.0 0.8001514622792103 0.0 64-65 0.0 0.0 0.0 0.941664748639438 0.0 66-67 0.0 0.0 0.0 1.1519300303667022 0.0 68-69 0.0 0.0 0.0 1.4051096245368893 0.0 70-71 0.0 0.0 0.0 1.6023818185867975 0.0 72-73 0.0 0.0 0.0 1.9489631516033468 0.0 74-75 0.0 0.0 0.0 2.233474648629786 0.0 76-77 0.0 0.0 0.0 2.5010580085680134 0.0 78-79 0.0 0.0 0.0 2.8433330610972103 0.0 80-81 0.0 0.0 0.0 3.1552414115690453 0.0 82-83 0.0 0.0 0.0 3.550008538314759 0.0 84-85 0.0 0.0 0.0 4.023402407062299 0.0 86-87 0.0 0.0 0.0 4.624277027478524 0.0 88-89 0.0 0.0 0.0 5.36725890397737 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTAC 55 0.002577328 34.549667 1 GTATCAA 9285 0.0 33.410107 1 GGTATCA 5980 0.0 32.888622 1 GTATAGG 205 3.092282E-11 27.80827 1 CGATCCG 35 0.008396201 27.140121 70-71 ATCAACG 11615 0.0 26.620216 3 TATCAAC 11700 0.0 26.467417 2 TAACGCG 45 8.951678E-4 26.387207 76-77 GGACCGA 90 8.948951E-4 26.386229 6 TCAACGC 11770 0.0 26.269653 4 CAACGCA 11870 0.0 26.048344 5 AACGCAG 12155 0.0 25.359434 6 GAGTACT 6645 0.0 24.165766 12-13 AACGCGA 50 0.0016544065 23.748486 76-77 GTACATG 7945 0.0 23.319395 1 AGTACTT 6770 0.0 22.84237 12-13 GTACTTT 7125 0.0 22.631052 14-15 ACGCAGA 13525 0.0 22.621813 7 CGCAGAG 13605 0.0 22.453875 8 TACATGG 8180 0.0 22.296041 2 >>END_MODULE