FastQCFastQC Report
Thu 26 May 2016
SRR937462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673410
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106931.5878885077441678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79151.1753612212470856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56070.8326279681026418No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.262247367873955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15780.2343297545328997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.196165783103904No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9630.1430035194012563No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9270.1376575934423308No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.13661810783920642No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.12102582379234048No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7100.10543353974547454No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.1036515644258327No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6950.10320607059592225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54050.040.026011
GTATCAA90600.038.258321
ATCAACG114300.030.4018963
TCAACGC115950.030.0512664
TATCAAC116000.029.7946512
CAACGCA117450.029.6696765
ATGGGCG650.00581820629.2555685
AACGCAG119800.029.0660276
GCGGGTC350.00837566127.15374850-51
GTGTAAG2700.026.415211
ACGCAGA133000.026.1455617
CGCAGAG133400.025.9940078
GTACTTG2407.2759576E-1225.7548311
GTACATG71950.025.3762251
GCAGAGT136000.025.2525719
TACATGG72750.024.8973942
GAGTACT74750.024.6217112-13
AGTACTT75200.024.09541512-13
GGCTAAC1000.001643367723.773691
ACGCAGG1401.7776554E-523.7506898
Produced by FastQC (version 0.11.5)