##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937462_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 673410 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.875612182771267 33.0 31.0 34.0 30.0 34.0 2 31.92962830964791 33.0 31.0 34.0 30.0 34.0 3 31.920558055270934 33.0 31.0 34.0 30.0 34.0 4 35.58245793795756 37.0 35.0 37.0 33.0 37.0 5 35.61685748652381 37.0 35.0 37.0 33.0 37.0 6 35.7139528667528 37.0 35.0 37.0 35.0 37.0 7 35.67949540398865 37.0 35.0 37.0 33.0 37.0 8 35.68057943897477 37.0 35.0 37.0 33.0 37.0 9 37.28512941595759 39.0 37.0 39.0 34.0 39.0 10-11 37.17794879790915 39.0 37.0 39.0 34.0 39.0 12-13 37.04872811511561 39.0 37.0 39.0 33.0 39.0 14-15 38.12250857575623 40.0 38.0 41.0 33.0 41.0 16-17 38.134254020581814 40.0 38.0 41.0 33.0 41.0 18-19 38.250100978601445 40.0 38.0 41.0 33.5 41.0 20-21 38.27940185028437 40.0 38.0 41.0 34.0 41.0 22-23 38.136547571316136 40.0 38.0 41.0 34.0 41.0 24-25 37.97338025868342 40.0 38.0 41.0 33.0 41.0 26-27 37.811056414368664 40.0 37.0 41.0 33.0 41.0 28-29 37.80676927874549 40.0 37.0 41.0 33.0 41.0 30-31 37.5019690827282 39.5 37.0 41.0 33.0 41.0 32-33 37.13769546041787 39.0 35.5 41.0 32.5 41.0 34-35 36.931632289392795 39.0 35.0 41.0 32.0 41.0 36-37 36.824371482454964 39.0 35.0 41.0 31.5 41.0 38-39 36.685152433138796 39.0 35.0 41.0 31.0 41.0 40-41 36.400420991669264 39.0 35.0 41.0 30.5 41.0 42-43 35.999483227157306 39.0 35.0 41.0 30.0 41.0 44-45 35.49705677076372 39.0 35.0 41.0 27.5 41.0 46-47 34.778726184642345 39.0 35.0 41.0 23.0 41.0 48-49 34.18938536701266 39.0 35.0 41.0 16.0 41.0 50-51 33.24790172406112 38.0 33.5 39.5 8.5 40.5 52-53 33.28902080456186 38.0 33.5 40.0 7.5 40.5 54-55 33.57996391499978 38.0 34.0 40.0 4.0 41.0 56-57 33.38999569355964 38.0 34.0 40.0 2.0 41.0 58-59 33.16851843601966 38.0 34.0 40.0 2.0 41.0 60-61 32.89315572979314 38.0 33.0 40.0 2.0 41.0 62-63 32.57921919781411 37.0 33.0 40.0 2.0 41.0 64-65 32.22673556971236 37.0 32.5 40.0 2.0 41.0 66-67 31.853469654445284 36.0 32.0 39.5 2.0 41.0 68-69 31.4094444691941 36.0 31.5 39.0 2.0 41.0 70-71 30.981779302356664 35.0 31.0 39.0 2.0 40.0 72-73 30.59466001395881 35.0 31.0 38.0 2.0 40.0 74-75 30.150641511115072 35.0 30.0 37.0 2.0 39.5 76-77 29.73539596976582 35.0 30.0 37.0 2.0 39.0 78-79 29.270807531815684 35.0 29.0 36.0 2.0 39.0 80-81 28.80070536523069 34.0 29.0 36.0 2.0 38.0 82-83 28.33660251555516 34.0 28.5 35.5 2.0 37.0 84-85 27.896757547407965 34.0 27.5 35.0 2.0 37.0 86-87 27.477024398212084 34.0 26.5 35.0 2.0 36.0 88-89 27.087284121114923 34.0 26.0 35.0 2.0 36.0 90-91 26.671979923078062 34.0 25.0 35.0 2.0 35.5 92-93 26.254164624819946 33.0 24.5 35.0 2.0 35.0 94-95 25.767444053399863 33.0 21.5 35.0 2.0 35.0 96-97 25.205478089128466 33.0 18.5 35.0 2.0 35.0 98-99 24.545114417665314 32.0 3.5 35.0 2.0 35.0 100-101 22.827122406854663 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21840433290029537 1101 2 0.26092143817923485 1101 3 0.274684749352506 1101 4 0.20724620566002727 1101 5 0.03425512636930961 1101 6 0.28182654730768064 1101 7 0.20880449946317015 1101 8 0.24464630827012002 1101 9 0.390335120016843 1101 10-11 0.3236280612075788 1101 12-13 0.1938964353274102 1101 14-15 0.4105825304869839 1101 16-17 0.47897484069520146 1101 18-19 0.40982913069346694 1101 20-21 0.3668092660466158 1101 22-23 0.3620447145099348 1101 24-25 0.4434380229705468 1101 26-27 0.437047036613869 1101 28-29 0.37600491688400695 1101 30-31 0.32950370124105177 1101 32-33 0.4724232199690803 1101 34-35 0.5497678258301804 1101 36-37 0.6211843352498292 1101 38-39 0.6668792889305806 1101 40-41 0.4383872840265184 1101 42-43 0.49582193091426063 1101 44-45 0.5735790523676414 1101 46-47 0.406895371080239 1101 48-49 0.6869789080181619 1101 50-51 0.5615395340199356 1101 52-53 0.5972438565146376 1101 54-55 0.7848571718225656 1101 56-57 0.9946254155862064 1101 58-59 0.8490614481466281 1101 60-61 0.8307568485020411 1101 62-63 0.7538033944456757 1101 64-65 0.7295651234601515 1101 66-67 0.6683278605861211 1101 68-69 0.9339283694092941 1101 70-71 0.9216186745615751 1101 72-73 0.8918263231623804 1101 74-75 0.7942727987850873 1101 76-77 0.8542676662188349 1101 78-79 0.7593193041026005 1101 80-81 0.7319830522673456 1101 82-83 0.4812911179192483 1101 84-85 0.7164938091553736 1101 86-87 0.9123405196105594 1101 88-89 0.7310224133424761 1101 90-91 0.9214262335741381 1101 92-93 0.9592433198053847 1101 94-95 1.1875912418999057 1101 96-97 1.211379025100868 1101 98-99 1.1124536005126373 1101 100-101 1.3982307799977 1102 1 0.2958448375111935 1102 2 0.31233745496544785 1102 3 0.27154739544983997 1102 4 0.16630510925087805 1102 5 0.25564620896678036 1102 6 0.2636042836199408 1102 7 0.38911911294665913 1102 8 0.26857983661332696 1102 9 0.31311779743283097 1102 10-11 0.47036292644771294 1102 12-13 0.41791813886484164 1102 14-15 0.5778574596449815 1102 16-17 0.5304356768228828 1102 18-19 0.6180232039301004 1102 20-21 0.4149360919774452 1102 22-23 0.417409406747403 1102 24-25 0.5559382287736412 1102 26-27 0.5745463734705538 1102 28-29 0.553598356064164 1102 30-31 0.8576493330016888 1102 32-33 0.7634809381340872 1102 34-35 0.537150723867093 1102 36-37 0.8509237062057977 1102 38-39 0.9605301261375843 1102 40-41 1.045658261765027 1102 42-43 1.1067662652921655 1102 44-45 1.1590861237621084 1102 46-47 1.5252906923068608 1102 48-49 1.2489091764402929 1102 50-51 1.2415523029588513 1102 52-53 1.2748372956947875 1102 54-55 1.2886512662797855 1102 56-57 1.13271929472765 1102 58-59 1.3368074743174674 1102 60-61 1.1374382232301556 1102 62-63 0.9779687028118929 1102 64-65 1.2371661179007312 1102 66-67 1.222800739396451 1102 68-69 1.11505064571962 1102 70-71 1.2549913391537437 1102 72-73 1.6564077013662803 1102 74-75 1.5587435006559751 1102 76-77 1.4459442571682537 1102 78-79 1.354127760872057 1102 80-81 1.3876598334703019 1102 82-83 1.25662182063164 1102 84-85 1.6863871025354626 1102 86-87 1.345541407216455 1102 88-89 1.2331673389698103 1102 90-91 1.3061755836936904 1102 92-93 1.2967055206362765 1102 94-95 1.4207835315096844 1102 96-97 1.5983510486849895 1102 98-99 1.7376898167357773 1102 100-101 1.9721708025720126 1103 1 0.30122962420398025 1103 2 0.13819318684152293 1103 3 0.16701134554967112 1103 4 0.14475834878347626 1103 5 0.05090630234876414 1103 6 0.20900456418465296 1103 7 0.212228203890362 1103 8 0.3243787132124396 1103 9 0.20404779139897755 1103 10-11 0.31894934856244817 1103 12-13 0.30954097919231316 1103 14-15 0.22071133948943356 1103 16-17 0.357399201507441 1103 18-19 0.28924857124335546 1103 20-21 0.19667426076928507 1103 22-23 0.04679085102752367 1103 24-25 0.19377277934667347 1103 26-27 0.25929271366192097 1103 28-29 0.14056485009026431 1103 30-31 0.3361568201202658 1103 32-33 0.42433645111638896 1103 34-35 0.34815784457654075 1103 36-37 0.6581125582317071 1103 38-39 0.3765339253720299 1103 40-41 0.30889039004058816 1103 42-43 0.6182324918871842 1103 44-45 1.0171325635025639 1103 46-47 0.9692624531073619 1103 48-49 0.8624883940949246 1103 50-51 0.8027321594026091 1103 52-53 0.647903654768129 1103 54-55 0.5050848586691288 1103 56-57 0.5041429988289536 1103 58-59 0.6778315926038516 1103 60-61 0.5469884631567581 1103 62-63 0.3835103316480044 1103 64-65 0.6701570252439417 1103 66-67 0.5428139110910131 1103 68-69 0.5936399800731884 1103 70-71 0.7667459344953045 1103 72-73 0.970461252299053 1103 74-75 0.8172646543593736 1103 76-77 0.9920927737347078 1103 78-79 1.087658859966215 1103 80-81 1.034804982879571 1103 82-83 0.8851543377767364 1103 84-85 0.9812979176006849 1103 86-87 0.9382467248122808 1103 88-89 1.059558884721465 1103 90-91 1.169818090198639 1103 92-93 1.057341378480956 1103 94-95 0.8535831447339142 1103 96-97 0.979998043463894 1103 98-99 1.1206111539292536 1103 100-101 1.2701280478605632 1104 1 0.05004745922156317 1104 2 0.0017580651183770613 1104 3 0.09301047420110109 1104 4 0.17195611303055358 1104 5 -0.06311815916843955 1104 6 0.0023143073493443467 1104 7 -0.014343677053673787 1104 8 0.055833334016419656 1104 9 0.234151821917429 1104 10-11 0.1777749407075575 1104 12-13 0.02658381341293392 1104 14-15 0.22218572502929845 1104 16-17 0.3795309239085398 1104 18-19 0.3014235422869618 1104 20-21 0.18462946070127373 1104 22-23 0.3158755099795627 1104 24-25 0.19074696830179505 1104 26-27 0.12456044082355788 1104 28-29 0.19426170579706792 1104 30-31 0.10795381715554697 1104 32-33 0.23759423215286546 1104 34-35 0.5855711082019823 1104 36-37 0.6696871488572711 1104 38-39 0.7970711861496511 1104 40-41 0.7803988856891522 1104 42-43 0.9123073216022917 1104 44-45 0.850484492689553 1104 46-47 1.1602604660075713 1104 48-49 1.226172817145283 1104 50-51 1.1912207946794737 1104 52-53 0.9548707241678542 1104 54-55 0.873317296213628 1104 56-57 1.200485991379992 1104 58-59 1.2697774627304028 1104 60-61 1.232880585953147 1104 62-63 1.0293071374116565 1104 64-65 1.0595435745973454 1104 66-67 1.393850497104804 1104 68-69 1.4654295387670118 1104 70-71 1.395663018240402 1104 72-73 1.3793335997333536 1104 74-75 1.2555078243679816 1104 76-77 1.0010373793432166 1104 78-79 1.1789910669221264 1104 80-81 1.2361396338619635 1104 82-83 1.1267357291716351 1104 84-85 1.3257010969603513 1104 86-87 1.4387302170073362 1104 88-89 1.137956347184403 1104 90-91 1.4234177161563295 1104 92-93 1.6435703518835716 1104 94-95 1.6567513468867787 1104 96-97 1.6814761481744895 1104 98-99 1.9013819786287982 1104 100-101 1.7689299435750137 1105 1 0.21461097969059395 1105 2 0.23256898648048363 1105 3 0.27491046397510743 1105 4 0.17970059951520057 1105 5 0.2389883956940153 1105 6 0.2688412187581477 1105 7 0.3359273117999919 1105 8 0.2938706248096139 1105 9 0.15093949618542268 1105 10-11 0.2762052505551935 1105 12-13 0.3643160287042022 1105 14-15 0.4177169654424304 1105 16-17 0.3660922628320762 1105 18-19 0.3541862651284191 1105 20-21 0.3942521215050334 1105 22-23 0.3613692550796088 1105 24-25 0.425753956064689 1105 26-27 0.6678385537867086 1105 28-29 0.5223269476418366 1105 30-31 0.31560456535748216 1105 32-33 0.38678978725393875 1105 34-35 0.2568070900678876 1105 36-37 0.39953341807362364 1105 38-39 0.4827820631991102 1105 40-41 0.4283025568211727 1105 42-43 0.49955380215585876 1105 44-45 0.37668178620968007 1105 46-47 0.5900215186032725 1105 48-49 0.43652663655421975 1105 50-51 0.39152757451381603 1105 52-53 0.33707238077895596 1105 54-55 0.30194326342227384 1105 56-57 0.26170561165472606 1105 58-59 0.3338501354645018 1105 60-61 0.4774983041929133 1105 62-63 0.5058862013922933 1105 64-65 0.5265988290541692 1105 66-67 0.43367392826681694 1105 68-69 0.49900829596781904 1105 70-71 0.3744645316875861 1105 72-73 0.44218426612733097 1105 74-75 0.3640363037626031 1105 76-77 0.2774766880659918 1105 78-79 0.5220595469984808 1105 80-81 0.45444586830993217 1105 82-83 0.2567353883051524 1105 84-85 0.46176306648688836 1105 86-87 0.5008122294830493 1105 88-89 0.41941832352826935 1105 90-91 0.5400434788450852 1105 92-93 0.7046195389170116 1105 94-95 0.7228726271812889 1105 96-97 1.0381775832223852 1105 98-99 1.0869360570962634 1105 100-101 1.3617189131356398 1106 1 0.013258907105647921 1106 2 0.11148732841251885 1106 3 0.0584187365244766 1106 4 0.14613006046521093 1106 5 0.07730897922007784 1106 6 0.013406242775225508 1106 7 -0.10726784920338872 1106 8 -0.22383085179597373 1106 9 -0.10243179006258174 1106 10-11 -0.33752006522491484 1106 12-13 -0.3668616976790062 1106 14-15 -0.16375430301514626 1106 16-17 -0.5192484238784729 1106 18-19 -0.1862602746331916 1106 20-21 -0.2525456013200795 1106 22-23 -0.08976933236034768 1106 24-25 0.0663339240419134 1106 26-27 0.015250046403679107 1106 28-29 0.2128357539040664 1106 30-31 0.457865728851516 1106 32-33 0.280029424329193 1106 34-35 0.2096713490965314 1106 36-37 0.29855494449240894 1106 38-39 0.1251677596943921 1106 40-41 0.24335325954311315 1106 42-43 0.3380344995406688 1106 44-45 0.3495424871525685 1106 46-47 0.5182549290284584 1106 48-49 0.7845430037486665 1106 50-51 0.3248579427756866 1106 52-53 0.43270483052099706 1106 54-55 0.6374707496346872 1106 56-57 0.48260047022081665 1106 58-59 0.554237966811236 1106 60-61 0.42750719940729454 1106 62-63 0.46100094965773053 1106 64-65 0.7238857886699002 1106 66-67 0.8210338286404131 1106 68-69 0.3216999878375937 1106 70-71 0.6063360847920158 1106 72-73 0.6340184593313865 1106 74-75 0.436647938703004 1106 76-77 0.6340057754169983 1106 78-79 0.48974323317532154 1106 80-81 0.34999826112260024 1106 82-83 0.2638604550192589 1106 84-85 0.08998758169770582 1106 86-87 0.4795348766988461 1106 88-89 0.49767841959658554 1106 90-91 0.4238186078256909 1106 92-93 0.37708885042741613 1106 94-95 0.48941772591816957 1106 96-97 0.21416050441078482 1106 98-99 0.6554825790674421 1106 100-101 0.8808614144699227 1107 1 0.20462721345682766 1107 2 0.17185470076619325 1107 3 0.12503221722186098 1107 4 0.2000333917229895 1107 5 0.186512746343368 1107 6 0.20808634862827802 1107 7 0.2660003637480486 1107 8 0.30925211831610966 1107 9 0.31871547021139435 1107 10-11 0.3342206726331156 1107 12-13 0.4267754442886158 1107 14-15 0.27715284206580293 1107 16-17 0.5136166134814246 1107 18-19 0.31378447941413157 1107 20-21 0.11578215397257452 1107 22-23 0.40629620313155357 1107 24-25 0.3208229495711805 1107 26-27 0.4861015407996945 1107 28-29 0.5631548697197601 1107 30-31 0.507690604318519 1107 32-33 0.5159902742669189 1107 34-35 0.7609264082497091 1107 36-37 0.8002030609307624 1107 38-39 0.87273742034197 1107 40-41 0.7391385957822081 1107 42-43 0.5370538021558602 1107 44-45 0.6241046758200639 1107 46-47 0.695926551070805 1107 48-49 0.7355120261646135 1107 50-51 0.6007199446436857 1107 52-53 0.5194790366231103 1107 54-55 0.6200439127729211 1107 56-57 0.6482721700962841 1107 58-59 0.8835051679320287 1107 60-61 1.0131314210760323 1107 62-63 1.0186500000935936 1107 64-65 1.1265379524307981 1107 66-67 1.2900659737213722 1107 68-69 1.2882940102535372 1107 70-71 1.0023257329862894 1107 72-73 0.6680568310623975 1107 74-75 0.7944137388275365 1107 76-77 0.6573265257283261 1107 78-79 0.7945027288166635 1107 80-81 1.0790806085696687 1107 82-83 0.9822426934999591 1107 84-85 1.1283418002531214 1107 86-87 1.167675054158373 1107 88-89 0.8515408884633366 1107 90-91 0.9753519204035257 1107 92-93 0.7532193765793487 1107 94-95 0.7723450297786911 1107 96-97 0.7021994988067988 1107 98-99 0.8547120311222365 1107 100-101 1.1799819001486256 1108 1 -0.017987614375886807 1108 2 -0.19078380751451718 1108 3 -0.07998051660394623 1108 4 0.03859491620767841 1108 5 -0.09135832180866288 1108 6 -0.04712446693230987 1108 7 0.034592866428852176 1108 8 -0.10106563159452975 1108 9 -0.0561616595395904 1108 10-11 0.009572342179168913 1108 12-13 0.17461032156730028 1108 14-15 0.2466875361561165 1108 16-17 0.18951654497195847 1108 18-19 0.28563509153137545 1108 20-21 0.170935071582484 1108 22-23 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98-99 -3.9789933260206176 2316 100-101 -5.016649593357869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2334.0 3 426.0 4 373.0 5 366.0 6 423.0 7 530.0 8 710.0 9 941.0 10 1273.0 11 1967.0 12 3110.0 13 4735.0 14 5386.0 15 7636.0 16 10675.0 17 15703.0 18 11759.0 19 5771.0 20 4915.0 21 5070.0 22 5146.0 23 5323.0 24 5965.0 25 6457.0 26 7314.0 27 8371.0 28 9780.0 29 11607.0 30 14001.0 31 17363.0 32 21999.0 33 28684.0 34 40156.0 35 58919.0 36 84266.0 37 124949.0 38 119648.0 39 19357.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.97438400154977 19.46741373786444 15.713679002779166 18.84452325780662 2 14.91059659365947 21.644635423260524 42.16102294476426 21.283745038315747 3 18.347514360989937 29.571947926611138 31.276798865716366 20.803738846682563 4 11.248586889011024 17.752248689660718 39.23004627705763 31.769118144270625 5 11.172839046477163 39.87905958400667 33.3597456080905 15.588355761425667 6 26.798339799424674 38.66097591101944 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18.83754757059793 26.140299318108262 36.098745959056735 18.923407152237072 30-31 18.995141719082874 26.02748288365649 36.09020374420247 18.88717165305817 32-33 18.65626276522843 26.202265951280634 35.84092953556704 19.300541747923894 34-35 19.05389799028479 26.16127131155348 35.82565899133251 18.959171706829224 36-37 19.180909105787585 25.954864598258325 35.59583164090455 19.268394655049537 38-39 19.12250984383032 26.339880512675162 35.511659722237724 19.025949921256796 40-41 19.09793822102009 26.431848816760844 35.28047811610116 19.189734846117908 42-43 18.995400090912 26.68475483146686 34.67340496683051 19.646440110790632 44-45 19.24369147718903 26.80439599028073 34.208791682972084 19.743120849558153 46-47 19.541900904413144 27.24116791014794 33.27140727161262 19.945523913826303 48-49 19.60099617023588 27.675421724219778 32.451874962331104 20.271707143213234 50-51 19.732818008777585 27.974265521126174 32.04211989481188 20.25079657528436 52-53 19.807656810089195 28.403332698193424 31.397744459396698 20.391266032320683 54-55 19.61792444055879 28.23899254078387 31.462490001674137 20.680593016983202 56-57 19.92912475619898 28.455354100989783 30.962915465948193 20.652605676863043 58-59 20.02127422174285 27.986313088109494 31.330977795960873 20.66143489418678 60-61 20.090717588322455 28.070012317835868 31.72452677492874 20.114743318912936 62-63 20.16246796576568 28.029369209196105 31.818874998605185 19.989287826433035 64-65 20.142520818378937 28.114611731189076 31.726056539704615 20.01681091072737 66-67 20.061662966144876 28.255399228655055 31.31593530223407 20.367002502966 68-69 20.2812604131956 28.19848740895892 31.310768434331415 20.209483743514067 70-71 20.125523697302956 28.400461805803516 31.1556005855888 20.31841391130472 72-73 20.025648826796775 28.357426220569497 31.026823406190978 20.59010154644275 74-75 19.77002738336035 28.843211106292223 30.892090516103067 20.49467099424436 76-77 19.532411805534373 28.512807083975282 31.22881201472679 20.725969095763556 78-79 19.548980691005504 28.473476541461224 31.054258006730922 20.923284760802353 80-81 19.55444992136739 28.717046091951797 30.871445724156505 20.85705826252431 82-83 19.53065549491462 28.684123025150576 30.769751469677008 21.0154700102578 84-85 19.487005167287876 28.31215565980546 30.708581178933052 21.492257993973602 86-87 19.439279030535282 28.45898429945582 30.5285879626186 21.573148707390295 88-89 19.452095552274443 27.938068796745004 30.720509969168287 21.889325681812267 90-91 19.343037458677625 27.885945780282494 30.98880613668891 21.782210624350967 92-93 19.437326265818783 27.90443383391589 31.06153304863925 21.59670685162608 94-95 19.097699507887487 27.744404972300075 31.163010038619227 21.99488548119321 96-97 19.02254520570362 27.86106916788777 30.997612335522646 22.118773290885965 98-99 18.98299990998946 27.9495375615475 30.70147966914803 22.36598285931501 100-101 18.967860173793518 27.703427896069005 31.46759055061887 21.861121379518604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1557.0 1 998.5 2 622.5 3 817.5 4 695.5 5 641.0 6 1073.0 7 2339.0 8 3944.5 9 4508.5 10 3757.0 11 2950.5 12 2779.0 13 2757.0 14 2829.5 15 2922.0 16 2899.5 17 2835.5 18 2927.0 19 3161.0 20 3329.0 21 3661.0 22 4055.0 23 4498.5 24 5105.0 25 5895.0 26 6855.0 27 7826.0 28 8774.0 29 9982.0 30 11413.5 31 12582.5 32 13767.0 33 15321.0 34 16992.0 35 18174.0 36 19783.5 37 21647.5 38 21956.5 39 21402.0 40 21213.0 41 21224.0 42 20971.5 43 21249.0 44 22132.5 45 22548.0 46 22331.5 47 22624.0 48 25040.0 49 24415.0 50 21032.5 51 18348.5 52 15662.5 53 14524.0 54 13751.0 55 12655.5 56 11602.0 57 10613.0 58 9795.5 59 8901.5 60 7850.0 61 6861.5 62 5644.0 63 3878.5 64 2422.5 65 1776.5 66 1291.5 67 906.5 68 668.5 69 498.0 70 354.5 71 269.5 72 232.0 73 228.0 74 187.5 75 139.0 76 99.0 77 65.5 78 46.5 79 29.5 80 14.0 81 8.5 82 7.0 83 4.0 84 2.5 85 1.0 86 2.0 87 2.5 88 3.5 89 4.0 90 3.0 91 2.5 92 2.5 93 2.0 94 0.5 95 1.0 96 3.0 97 4.0 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3482276770466729 2 0.2992233557565228 3 0.29298644213777636 4 0.2998173475297367 5 0.29773837632348793 6 0.21428253218692922 7 0.2012147131762225 8 0.206263643248541 9 0.19972973374318767 10-11 0.2205194458056756 12-13 0.2099018428594764 14-15 0.2053726555887201 16-17 0.20574390044697882 18-19 0.2015859580344812 20-21 0.19430955881261044 22-23 0.19661127693381447 24-25 0.18681041267578444 26-27 0.18755290239230185 28-29 0.20529840661706833 30-31 0.21747523796795415 32-33 0.2106443325759938 34-35 0.21888596842933725 36-37 0.19289882835122732 38-39 0.19133959994654073 40-41 0.18888938388203325 42-43 0.1977250115085906 44-45 0.1994327378565807 46-47 0.20373917821238174 48-49 0.21331729555545656 50-51 0.2184404745994268 52-53 0.24383362290432276 54-55 0.21123832434920775 56-57 0.18458294352623217 58-59 0.18339495997980426 60-61 0.1802765031704311 62-63 0.1897061225702024 64-65 0.18228122540502814 66-67 0.17931126653895843 68-69 0.1761185607579335 70-71 0.18799839622221232 72-73 0.18629066987422224 74-75 0.19104260405993376 76-77 0.19327007320948605 78-79 0.191265350974889 80-81 0.19260183246462037 82-83 0.18391470278136648 84-85 0.17827178093583404 86-87 0.17879152373739624 88-89 0.18005375625547587 90-91 0.1865134167891775 92-93 0.18213272746172465 94-95 0.1790885196240032 96-97 0.17894002168069972 98-99 0.18807264519386407 100-101 0.1745593323532469 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 673410.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.916923341102745 #Duplication Level Percentage of deduplicated Percentage of total 1 79.74028177575855 46.1831178680454 2 11.685187774387042 13.535402491111304 3 3.6532321405121513 6.347518974878848 4 1.6440389859735394 3.8087071968165507 5 0.8636814370082182 2.5010885789169217 6 0.5167327101264275 1.795654125613954 7 0.35384762970721917 1.4345636230928747 8 0.2461530376883501 1.1405141291180598 9 0.20112831908862028 1.0483860094542425 >10 0.9934789539274446 10.438399817976483 >50 0.06293502606231703 2.5309701361678028 >100 0.03467842037665789 3.665081093114818 >500 0.00308252625566306 1.2739634422614896 >1k 7.70631563915765E-4 0.6940370732395013 >5k 5.137543759438434E-4 2.0117404725283032 >10k+ 2.568771879719217E-4 1.5908549676634482 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10693 1.5878885077441678 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7915 1.1753612212470856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5607 0.8326279681026418 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1766 0.262247367873955 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1578 0.2343297545328997 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1321 0.196165783103904 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 963 0.1430035194012563 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 927 0.1376575934423308 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 920 0.13661810783920642 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 815 0.12102582379234048 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 710 0.10543353974547454 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.1036515644258327 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 695 0.10320607059592225 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.4849794330348525E-4 0.0 16-17 0.0 0.0 0.0 1.4849794330348525E-4 0.0 18-19 0.0 0.0 0.0 1.4849794330348525E-4 0.0 20-21 0.0 0.0 0.0 1.4849794330348525E-4 0.0 22-23 0.0 0.0 0.0 4.454938299104557E-4 0.0 24-25 0.0 0.0 0.0 0.0011137345747761393 0.0 26-27 0.0 0.0 0.0 0.0019304732629453081 0.0 28-29 0.0 0.0 0.0 0.002969958866069705 0.0 30-31 0.0 0.0 0.0 0.004900432129015014 0.0 32-33 0.0 0.0 0.0 0.00735064819352252 0.0 34-35 0.0 0.0 0.0 0.009281121456467829 0.0 36-37 0.0 0.0 0.0 0.012102582379234048 0.0 38-39 0.0 0.0 0.0 0.01529528816025898 0.0 40-41 0.0 0.0 0.0 0.023611172985254156 0.0 42-43 0.0 0.0 0.0 0.03304079238502547 0.0 44-45 0.0 0.0 0.0 0.052791018844389005 0.0 46-47 0.0 0.0 0.0 0.09674641006222065 0.0 48-49 0.0 0.0 0.0 0.12882196581577346 0.0 50-51 0.0 0.0 0.0 0.16891641050771447 0.0 52-53 0.0 0.0 0.0 0.21725249105299893 0.0 54-55 0.0 0.0 0.0 0.26536582468332814 0.0 56-57 0.0 0.0 0.0 0.31927057810249326 0.0 58-59 0.0 0.0 0.0 0.41282428238368896 0.0 60-61 0.0 0.0 0.0 0.5368943140137509 0.0 62-63 0.0 0.0 0.0 0.7023210228538335 0.0 64-65 0.0 0.0 0.0 0.8306974948396965 0.0 66-67 0.0 0.0 0.0 1.0198096256366849 0.0 68-69 0.0 0.0 0.0 1.2420367977903506 0.0 70-71 0.0 0.0 0.0 1.4206798235844433 0.0 72-73 0.0 0.0 0.0 1.7546516980739817 0.0 74-75 0.0 0.0 0.0 2.019126535097489 0.0 76-77 0.0 0.0 0.0 2.255980754666548 0.0 78-79 0.0 0.0 0.0 2.556911836771061 0.0 80-81 0.0 0.0 0.0 2.8422506348287078 0.0 82-83 0.0 0.0 0.0 3.223222108373799 0.0 84-85 0.0 0.0 0.0 3.6849022141043344 0.0 86-87 0.0 0.0 0.0 4.2631532053281065 0.0 88-89 0.0 0.0 0.0 4.996436049360716 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5405 0.0 40.02601 1 GTATCAA 9060 0.0 38.25832 1 ATCAACG 11430 0.0 30.401896 3 TCAACGC 11595 0.0 30.051266 4 TATCAAC 11600 0.0 29.794651 2 CAACGCA 11745 0.0 29.669676 5 ATGGGCG 65 0.005818206 29.255568 5 AACGCAG 11980 0.0 29.066027 6 GCGGGTC 35 0.008375661 27.153748 50-51 GTGTAAG 270 0.0 26.41521 1 ACGCAGA 13300 0.0 26.145561 7 CGCAGAG 13340 0.0 25.994007 8 GTACTTG 240 7.2759576E-12 25.754831 1 GTACATG 7195 0.0 25.376225 1 GCAGAGT 13600 0.0 25.252571 9 TACATGG 7275 0.0 24.897394 2 GAGTACT 7475 0.0 24.62171 12-13 AGTACTT 7520 0.0 24.095415 12-13 GGCTAAC 100 0.0016433677 23.77369 1 ACGCAGG 140 1.7776554E-5 23.750689 8 >>END_MODULE