FastQCFastQC Report
Thu 26 May 2016
SRR937462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673410
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94601.4047905436509704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75161.1161105418689952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54590.8106502724937259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22850.3393178004484638No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.2625443637605619No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.2165100013364815No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12890.1914138489181925No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.13260866337001231No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.1167193834365394No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.11048246981779303No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.10513654385886756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59700.033.9103781
GTATCAA90500.031.7165341
ATGGGCG1004.9518203E-528.4985525
GTCTAGG1309.976657E-625.5889231
GTACATG75950.025.4661881
GTCTAGC950.001222678825.0117281
ATCAACG114900.024.8855923
TATCAAC116150.024.6177752
TCAACGC116400.024.5649034
TACATGG77200.024.5486262
CAACGCA117600.024.354635
AACGCAG120300.023.84756
CTGGTCG1000.001652711823.7505579
ACATGGG76950.023.5173243
AGTACTT68650.023.38729312-13
GAGTACT68050.023.27938712-13
GTATAGC2103.423702E-822.6296621
ATGGGAG18900.022.6178995
GTACTTT72650.021.60764314-15
CATGGGA60400.021.468284
Produced by FastQC (version 0.11.5)