##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937462_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 673410 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.254209174202934 33.0 31.0 34.0 31.0 34.0 2 32.43840750805602 34.0 31.0 34.0 31.0 34.0 3 32.48644362275582 34.0 31.0 34.0 31.0 34.0 4 36.11782420813472 37.0 35.0 37.0 35.0 37.0 5 36.01067403216465 37.0 35.0 37.0 35.0 37.0 6 36.127604282680686 37.0 35.0 37.0 35.0 37.0 7 36.09511590264475 37.0 35.0 37.0 35.0 37.0 8 36.08202432396311 37.0 35.0 37.0 35.0 37.0 9 37.762970552857844 39.0 37.0 39.0 35.0 39.0 10-11 37.69831603332294 39.0 37.0 39.0 35.0 39.0 12-13 37.6164795592581 39.0 37.0 39.0 35.0 39.0 14-15 38.72393564099137 40.0 38.0 41.0 34.5 41.0 16-17 38.736433227899795 40.0 38.0 41.0 34.5 41.0 18-19 38.86108165901902 40.0 38.0 41.0 35.0 41.0 20-21 38.84287209872143 40.0 38.0 41.0 35.0 41.0 22-23 38.71836399815862 40.0 38.0 41.0 35.0 41.0 24-25 38.566997817080235 40.0 38.0 41.0 34.0 41.0 26-27 38.33416640679526 40.0 38.0 41.0 34.0 41.0 28-29 38.26668151646099 40.0 38.0 41.0 34.0 41.0 30-31 38.203790410002824 40.0 37.0 41.0 34.0 41.0 32-33 38.00089321512897 40.0 36.5 41.0 34.0 41.0 34-35 37.82065977636209 40.0 36.0 41.0 33.0 41.0 36-37 37.6064552055954 40.0 36.0 41.0 33.0 41.0 38-39 37.417117357924596 40.0 35.5 41.0 33.0 41.0 40-41 37.167232443830656 40.0 35.0 41.0 33.0 41.0 42-43 36.82814184523544 40.0 35.0 41.0 32.5 41.0 44-45 36.30590353573603 40.0 35.0 41.0 30.5 41.0 46-47 35.6339518272672 39.0 35.0 41.0 28.0 41.0 48-49 35.054701444884984 39.0 35.0 41.0 24.0 41.0 50-51 34.738855229355075 39.0 35.0 41.0 22.5 41.0 52-53 34.47565079223653 39.0 35.0 41.0 20.5 41.0 54-55 34.268214015235884 39.0 35.0 40.0 19.5 41.0 56-57 34.02825990110037 38.0 34.0 40.0 17.0 41.0 58-59 33.77812848042055 38.0 34.0 40.0 13.0 41.0 60-61 33.4476492775575 38.0 34.0 40.0 8.5 41.0 62-63 33.07954069586136 37.0 33.0 40.0 6.5 41.0 64-65 32.75534518346921 37.0 33.0 40.0 2.0 41.0 66-67 32.38180900194533 36.0 33.0 39.0 2.0 41.0 68-69 31.960160229280824 36.0 32.0 39.0 2.0 40.5 70-71 31.569548269256472 35.0 32.0 38.5 2.0 40.0 72-73 31.144607297188934 35.0 31.0 38.0 2.0 40.0 74-75 30.706825707963944 35.0 31.0 37.0 2.0 39.5 76-77 29.41221469832643 34.0 29.5 36.0 2.0 38.5 78-79 29.951803507521422 34.5 31.0 36.0 2.0 39.0 80-81 29.74596679585988 35.0 31.0 36.0 2.0 37.5 82-83 29.396259336808185 34.0 30.0 35.5 2.0 37.0 84-85 29.05311697182994 34.0 30.0 35.0 2.0 37.0 86-87 28.72052761319256 34.0 29.5 35.0 2.0 36.0 88-89 28.352256426248495 34.0 29.0 35.0 2.0 36.0 90-91 27.91555812951991 34.0 29.0 35.0 2.0 35.5 92-93 27.526494260554493 34.0 27.5 35.0 2.0 35.0 94-95 27.160121619815563 34.0 27.0 35.0 2.0 35.0 96-97 26.777639922187078 34.0 26.0 35.0 2.0 35.0 98-99 26.219278745489376 33.0 25.0 35.0 2.0 35.0 100-101 24.815443786103565 32.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09309606822885996 1101 2 0.11793044263985308 1101 3 0.051944394277782635 1101 4 -0.033966501563376994 1101 5 -0.03886591182470056 1101 6 -0.08336426960833165 1101 7 0.0068581108365037835 1101 8 0.019929178270992054 1101 9 -0.010024387329984563 1101 10-11 0.06716879612336868 1101 12-13 0.12464961490054094 1101 14-15 0.1430855334463459 1101 16-17 0.05206420774829468 1101 18-19 0.00422960715800258 1101 20-21 0.1784066200940373 1101 22-23 0.2081113095178324 1101 24-25 0.27978381764145155 1101 26-27 0.26279984763008457 1101 28-29 0.17809636224408365 1101 30-31 0.3125231981960823 1101 32-33 0.3015433126014173 1101 34-35 0.34117141734270007 1101 36-37 0.4393679952788645 1101 38-39 0.22082547637342032 1101 40-41 0.2442732847024729 1101 42-43 0.33304530076647154 1101 44-45 0.2934464383225901 1101 46-47 0.19882253629086222 1101 48-49 0.21998701731187964 1101 50-51 0.1737006716208782 1101 52-53 0.3021259435437287 1101 54-55 0.35512786519234396 1101 56-57 0.3379021067153474 1101 58-59 0.0800560272896007 1101 60-61 0.11261898557417993 1101 62-63 0.3401578148166564 1101 64-65 0.23752608571831502 1101 66-67 0.33648075896569196 1101 68-69 0.22460229874752713 1101 70-71 0.15241261747994983 1101 72-73 0.017784357055699473 1101 74-75 0.2353153944455677 1101 76-77 0.10677257438775811 1101 78-79 0.13959678430869715 1101 80-81 0.008205512407176485 1101 82-83 0.09173318122388352 1101 84-85 0.16759879146735557 1101 86-87 0.14855818747341587 1101 88-89 -0.15288941266700462 1101 90-91 0.1378313406122409 1101 92-93 0.18605863966130798 1101 94-95 0.011304282851114067 1101 96-97 -0.017524717613678575 1101 98-99 -0.05255980721834774 1101 100-101 0.24485489854881237 1102 1 0.22283982965092974 1102 2 0.10191987625143639 1102 3 0.10154385571394187 1102 4 0.044657599534417614 1102 5 0.08698862837459131 1102 6 0.15885591592545012 1102 7 0.018639194879170873 1102 8 0.21980148888182782 1102 9 0.25067600177507643 1102 10-11 0.15312040221075307 1102 12-13 0.23440120598716874 1102 14-15 0.23436515308563344 1102 16-17 0.217217078289913 1102 18-19 0.3021949919915059 1102 20-21 0.18066039269350398 1102 22-23 0.24858043082092252 1102 24-25 -0.05317724075357688 1102 26-27 -0.06115984057364443 1102 28-29 -0.0659312318353713 1102 30-31 0.03636836840896507 1102 32-33 0.14948592189201548 1102 34-35 0.06639684033667237 1102 36-37 0.017126808682228045 1102 38-39 0.11896026002406046 1102 40-41 -0.05586080744975419 1102 42-43 -0.028747399717758526 1102 44-45 -0.13915624827679807 1102 46-47 0.07567179146658276 1102 48-49 0.10112703842270321 1102 50-51 0.09718765297419907 1102 52-53 0.16273321818018616 1102 54-55 -0.05601733300975553 1102 56-57 -0.05572970513855324 1102 58-59 -0.12768162040842412 1102 60-61 -0.13327547127943262 1102 62-63 -0.20193029086185987 1102 64-65 0.050152883294046546 1102 66-67 -0.13982352960500322 1102 68-69 -0.20696496959593347 1102 70-71 -0.11201783845165991 1102 72-73 -0.12496616019608808 1102 74-75 -0.3038137200157145 1102 76-77 -0.25003328834797856 1102 78-79 -0.1730666004845567 1102 80-81 -0.03339598327827886 1102 82-83 0.08225251848302051 1102 84-85 0.14516168032087506 1102 86-87 0.3335312684281213 1102 88-89 0.180333244333319 1102 90-91 0.12625697434858196 1102 92-93 0.1284593831135652 1102 94-95 0.03555977870852445 1102 96-97 0.00473311616693195 1102 98-99 0.0014529873316213582 1102 100-101 0.28391541197723313 1103 1 0.1135490264250052 1103 2 0.039384781870154484 1103 3 0.010870443312370526 1103 4 0.05685093412401443 1103 5 0.0018142081099767893 1103 6 -0.019500629664413793 1103 7 0.10277434560180154 1103 8 0.045249888007013794 1103 9 -0.07725922089699111 1103 10-11 0.01719688940356434 1103 12-13 0.07124350159273263 1103 14-15 0.2341681486944367 1103 16-17 0.05173338968248231 1103 18-19 -0.013973263082554865 1103 20-21 -0.01888697306276299 1103 22-23 0.06294519158907264 1103 24-25 -0.05912353754687416 1103 26-27 -0.015804100132164933 1103 28-29 -0.09310356214035664 1103 30-31 0.06432300600653917 1103 32-33 0.12011323336261626 1103 34-35 0.1309181577345342 1103 36-37 0.008910678685971618 1103 38-39 0.20600955299718748 1103 40-41 0.17505879382097334 1103 42-43 0.36015925446244523 1103 44-45 0.31411469081553633 1103 46-47 0.30351070088072873 1103 48-49 0.3905683644240412 1103 50-51 0.3525472192980601 1103 52-53 0.3792039273388994 1103 54-55 0.4491799664121672 1103 56-57 0.3566747191803543 1103 58-59 0.43405498757568495 1103 60-61 0.44808163201368245 1103 62-63 0.41196108834018474 1103 64-65 0.4270165733861546 1103 66-67 0.5534501504179872 1103 68-69 0.41102352991583757 1103 70-71 0.5932198135605411 1103 72-73 0.8090145276633045 1103 74-75 0.6091503109796399 1103 76-77 0.2580010646496298 1103 78-79 0.6296853700352116 1103 80-81 0.5872211448735563 1103 82-83 0.9067901291909273 1103 84-85 0.571624794817545 1103 86-87 0.8240702990903763 1103 88-89 0.5768926149851872 1103 90-91 0.5209842993334988 1103 92-93 0.6704708074648345 1103 94-95 0.747447994419769 1103 96-97 0.9921924066846444 1103 98-99 0.9727950345403436 1103 100-101 1.2108515273551852 1104 1 0.16796049422229942 1104 2 0.18976679383525408 1104 3 0.19980431645677044 1104 4 0.12704231202364014 1104 5 0.005416490618188163 1104 6 0.03526681590286529 1104 7 -0.0706718379977147 1104 8 0.027578513932823512 1104 9 -0.09971769901232364 1104 10-11 0.1153856532574764 1104 12-13 0.10506670284236463 1104 14-15 0.10673076359024236 1104 16-17 0.05026166069313831 1104 18-19 0.06433918976367892 1104 20-21 -0.089386510822969 1104 22-23 -0.11967140441336 1104 24-25 -0.03437511288133521 1104 26-27 -0.17169314132994629 1104 28-29 -0.16499778892475803 1104 30-31 -0.21939924632325614 1104 32-33 -0.3945615517224468 1104 34-35 -0.3277777947450886 1104 36-37 -0.15208956571667187 1104 38-39 -0.22004536321520618 1104 40-41 -0.23143062779485035 1104 42-43 -0.1323386468626424 1104 44-45 0.02204821329527107 1104 46-47 -0.2947179112310181 1104 48-49 -0.5970467970411022 1104 50-51 -0.3392158801525582 1104 52-53 -0.384246187955398 1104 54-55 -0.24853646275888508 1104 56-57 -0.16055832405765358 1104 58-59 -0.2498075306216876 1104 60-61 -0.14719289591728568 1104 62-63 0.11884792991413917 1104 64-65 0.02850211643820444 1104 66-67 -0.1830306639741366 1104 68-69 -0.1945690402436604 1104 70-71 -0.030538249070293233 1104 72-73 0.03791620341537438 1104 74-75 -0.24853827754193603 1104 76-77 -0.1477312400673405 1104 78-79 0.019579320538358758 1104 80-81 -0.013690583265688616 1104 82-83 -0.10788996899518111 1104 84-85 0.004167007405342815 1104 86-87 1.5443735852116447E-4 1104 88-89 -0.23493594187030808 1104 90-91 -0.01200474505214899 1104 92-93 0.11084187444178539 1104 94-95 -0.1940175713723633 1104 96-97 -0.004125614291478286 1104 98-99 0.28666116564642863 1104 100-101 0.4566541775299875 1105 1 0.04158765446363333 1105 2 0.14458835023161498 1105 3 0.17989089654335544 1105 4 0.07782119193637271 1105 5 -0.07266920321553982 1105 6 0.04656313113618893 1105 7 0.06543273983914588 1105 8 -0.008522655239211474 1105 9 0.040286391385464526 1105 10-11 0.1532064938342188 1105 12-13 0.08793645775936909 1105 14-15 0.1675873610610168 1105 16-17 0.14659905033762044 1105 18-19 0.10340760166673846 1105 20-21 0.03489170440013112 1105 22-23 0.030727409371287706 1105 24-25 0.13672622672394397 1105 26-27 0.08760740064775518 1105 28-29 0.08517605298136033 1105 30-31 -0.03875023654038756 1105 32-33 -0.12211208517849315 1105 34-35 -0.148257053019627 1105 36-37 -0.1553925619330485 1105 38-39 -0.0387623417747065 1105 40-41 0.06880627441055509 1105 42-43 -0.1005221693768732 1105 44-45 0.17406614287225608 1105 46-47 0.1647564726528188 1105 48-49 -0.16090472389115007 1105 50-51 -0.09012940232592825 1105 52-53 -0.2625227670193766 1105 54-55 -0.2909027578810779 1105 56-57 -0.27080306619216543 1105 58-59 -0.3039780320362837 1105 60-61 -0.3451698533430658 1105 62-63 -0.1463700542014834 1105 64-65 -0.21713138391970688 1105 66-67 -0.34517403241672184 1105 68-69 -0.26614929725698744 1105 70-71 -0.355222095407683 1105 72-73 -0.1431333244845483 1105 74-75 -0.1707014162405045 1105 76-77 -0.25887416538349584 1105 78-79 -0.08182504936994306 1105 80-81 -0.11036372639552638 1105 82-83 -0.24731629070496908 1105 84-85 -0.09452747396630201 1105 86-87 0.015140632265975285 1105 88-89 -0.01768298485357178 1105 90-91 -0.2316744454305102 1105 92-93 -0.19039376655763718 1105 94-95 -0.28399950544878294 1105 96-97 -0.2874078176524222 1105 98-99 -0.1569629267965631 1105 100-101 -0.29989386760073344 1106 1 -0.10706916816579337 1106 2 0.03654707643691779 1106 3 0.0016308004750342775 1106 4 -0.08035351007394098 1106 5 -0.22813976005596004 1106 6 -0.04253133604046866 1106 7 0.03453076510155739 1106 8 0.011402624960041408 1106 9 0.0273883305422018 1106 10-11 -0.04829679739509629 1106 12-13 -0.2973152768074385 1106 14-15 -0.032910770943963996 1106 16-17 -0.10483307917670714 1106 18-19 -0.011456301553330661 1106 20-21 -0.3226425421752168 1106 22-23 -0.16450475572388967 1106 24-25 -0.1466930936816837 1106 26-27 0.058244297996985495 1106 28-29 -0.09381345066567093 1106 30-31 -0.1281649314345401 1106 32-33 -0.18448532837365406 1106 34-35 -0.3263036639429444 1106 36-37 -0.08952349948864935 1106 38-39 -0.17532167285458655 1106 40-41 0.003871209475491355 1106 42-43 -0.09876981214506486 1106 44-45 -0.08304113341310426 1106 46-47 0.2022942773139036 1106 48-49 0.15525788062761592 1106 50-51 0.42970692572958313 1106 52-53 0.4761536250816718 1106 54-55 0.47336516597588485 1106 56-57 0.4456797660440941 1106 58-59 0.21504883151112608 1106 60-61 0.26755919842707954 1106 62-63 0.42346627385402513 1106 64-65 0.3696681179482937 1106 66-67 0.6729988905507227 1106 68-69 0.6800435509906535 1106 70-71 0.5578538697230719 1106 72-73 0.5524813348287374 1106 74-75 0.5648349651862858 1106 76-77 0.4362690475679365 1106 78-79 0.5761895387816303 1106 80-81 0.7626837740492327 1106 82-83 0.7714704020543302 1106 84-85 0.6904396137405087 1106 86-87 0.6924044643243263 1106 88-89 0.4395310340042826 1106 90-91 0.5470126209277879 1106 92-93 0.44687718167221746 1106 94-95 0.3287829176076116 1106 96-97 0.393832175231406 1106 98-99 0.7083902132141127 1106 100-101 0.8644185323281057 1107 1 0.03724512199610075 1107 2 0.043046142439408186 1107 3 -0.07765780475534712 1107 4 -0.05651322364803946 1107 5 -0.06572926815060498 1107 6 0.044412157110215844 1107 7 0.0038255969820042424 1107 8 0.07272734476079279 1107 9 0.0986873654114433 1107 10-11 -0.007142532139809532 1107 12-13 0.04008743178533791 1107 14-15 -0.034401275302620604 1107 16-17 0.08196836851943345 1107 18-19 0.020696562705694532 1107 20-21 -0.01894352287259693 1107 22-23 -0.13100148673260748 1107 24-25 0.0236174604901791 1107 26-27 0.1339953876607396 1107 28-29 -0.05309505091474165 1107 30-31 -0.1025286456313026 1107 32-33 -0.04416971504862488 1107 34-35 -0.13626435821442584 1107 36-37 -0.27895181517980205 1107 38-39 -0.27297500411236797 1107 40-41 -0.21715151779723385 1107 42-43 -0.34912200703920604 1107 44-45 -0.11895333764722693 1107 46-47 -0.38067778059393476 1107 48-49 -0.33144043817686253 1107 50-51 -0.331971934793458 1107 52-53 -0.028452150136260457 1107 54-55 -0.10470145917978613 1107 56-57 0.19784547276887565 1107 58-59 0.054747617314362174 1107 60-61 0.0027806336699214285 1107 62-63 -0.03835463212356416 1107 64-65 -0.11372229301061054 1107 66-67 0.010386032518340471 1107 68-69 -0.12962332323101577 1107 70-71 -0.050534595407683014 1107 72-73 0.14172868850246445 1107 74-75 -0.1168256045521936 1107 76-77 -0.1612889381107685 1107 78-79 0.020062125954726895 1107 80-81 -0.12174765496695628 1107 82-83 -0.35153706992574385 1107 84-85 -0.36520523370656477 1107 86-87 -0.2960606664353236 1107 88-89 -0.023912692645779998 1107 90-91 -0.15308272465129136 1107 92-93 -0.27939538993426183 1107 94-95 -0.22338668077345858 1107 96-97 -0.004412687782291869 1107 98-99 -0.25661795926409425 1107 100-101 -0.161521302665669 1108 1 -0.11698496557687577 1108 2 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-0.725845939699262 2316 56-57 -0.3017486830752816 2316 58-59 -0.34318257749082903 2316 60-61 -0.5194393338625503 2316 62-63 -0.5490080412144707 2316 64-65 -0.23314193586775644 2316 66-67 -0.3730352337154237 2316 68-69 -0.3397491349193267 2316 70-71 -0.18872453047261573 2316 72-73 -0.7262704998092246 2316 74-75 -0.8716957344223246 2316 76-77 -0.7704610160328471 2316 78-79 -0.7484037831361796 2316 80-81 -0.8385698952266942 2316 82-83 -0.679459147847826 2316 84-85 -0.6748643246156547 2316 86-87 -0.7449243027989603 2316 88-89 -0.7149638290094167 2316 90-91 -0.9665973350409018 2316 92-93 -0.8440260717524417 2316 94-95 -0.6706269405137206 2316 96-97 -1.0256180449251495 2316 98-99 -1.1989880891342217 2316 100-101 -1.6892201663020323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 23.0 6 35.0 7 83.0 8 208.0 9 402.0 10 783.0 11 1400.0 12 2286.0 13 3447.0 14 4173.0 15 6313.0 16 9666.0 17 14531.0 18 12430.0 19 5876.0 20 4234.0 21 4019.0 22 4061.0 23 4246.0 24 4621.0 25 5100.0 26 5960.0 27 7188.0 28 8545.0 29 10713.0 30 13051.0 31 16441.0 32 20695.0 33 26975.0 34 37993.0 35 57374.0 36 85469.0 37 137660.0 38 138893.0 39 18508.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.250804866489474 20.502070293406707 15.90166589163756 18.34545894846626 2 15.295585156145588 22.72048232131985 40.55449132029521 21.42944120223935 3 19.05629557030635 29.610341396771656 30.98261089083916 20.350752142082833 4 11.820750836415908 18.42149421822399 38.119181656318815 31.638573289041283 5 11.526586162393682 40.9550018638457 32.561161613986414 14.9572503597742 6 27.353618152388588 38.78692030115383 18.017997950728383 15.841463595729198 7 25.915712567380943 37.14572103176371 20.327883458814096 16.61068294204125 8 27.64630759863976 33.55622874623187 19.454418556302997 19.34304509882538 9 28.918786474807323 16.166228597733923 20.619830415348748 34.29515451211001 10-11 24.312974265306426 29.22914717631161 25.684055775827503 20.77382278255446 12-13 26.790209260571068 27.25057954678773 27.365969131429775 18.593242061211427 14-15 23.391767274023252 26.908644065279695 28.71222583567218 20.987362825024874 16-17 19.43741554179475 30.76016097177054 28.764645609658306 21.037777876776406 18-19 19.511516015503187 27.308400528652676 34.571286437682836 18.6087970181613 20-21 21.46827341441321 25.66029610489895 34.729214000386094 18.142216480301748 22-23 20.58108108108108 26.292990792990796 34.56689931689932 18.55902880902881 24-25 19.84137355473518 26.52070222124543 35.002361131323454 18.635563092695936 26-27 19.667875718841536 26.77480072914385 34.982643851839754 18.574679700174862 28-29 19.297698496850245 26.917696536607533 35.03562592628893 18.748979040253285 30-31 19.458019812860393 26.763399519751236 35.007967021136 18.770613646252382 32-33 19.309707310553748 26.95215396266762 34.69869767303722 19.039441053741406 34-35 19.508471807665465 26.94420932270088 34.72913975141444 18.818179118219213 36-37 19.797730350693783 26.798760929481087 34.45667411138567 18.94683460843946 38-39 19.63034214846456 27.18588252415146 34.349015045147326 18.834760282236658 40-41 19.61184093779491 27.343317847461314 34.18343519062324 18.861406024120537 42-43 19.504609937504455 27.651382387187223 33.563137133759476 19.280870541548843 44-45 19.749186973760413 27.676378432158717 33.057943897477024 19.516490696603853 46-47 20.138400083158846 28.29205090509496 32.068799097132505 19.50074991461368 48-49 20.144874701054853 28.754272103378405 31.299825737534142 19.801027458032593 50-51 20.211980814065726 29.013008419833387 31.007707043257447 19.767303722843437 52-53 20.366612539045764 29.46532453926554 30.234842595541934 19.93322032614676 54-55 19.927161758809643 29.452488083040052 30.309989456646026 20.310360701504283 56-57 20.386614395390623 29.651252580151766 29.78089128465571 20.1812417398019 58-59 20.432723006786354 29.235903832731918 30.133573900001487 20.19779926048024 60-61 20.26291560861882 29.452636580983356 30.617974191057456 19.666473619340373 62-63 20.391663325462943 29.351954975423588 30.569192616682262 19.687189082431207 64-65 20.27189973418868 29.401924533345213 30.423813130188147 19.90236260227796 66-67 20.295807903060542 29.50327437964984 30.09674641006222 20.104171307227396 68-69 20.455294694168487 29.491914286987125 29.9530746499161 20.09971636892829 70-71 20.311177440192456 29.875038980710116 29.766709731070222 20.047073848027207 72-73 20.28303707993644 29.740128599218902 29.715552189602175 20.261282131242485 74-75 20.12815372507091 30.287269271320593 29.410760160971773 20.17381684263673 76-77 19.92196433079402 29.86197116169941 29.902733847136215 20.313330660370355 78-79 19.975572088326576 29.83323680967019 29.621849987377676 20.569341114625562 80-81 19.906520544690455 30.28184909639002 29.482261920672396 20.32936843824713 82-83 19.88284945062941 30.173728517820575 29.293725785446245 20.64969624610377 84-85 19.780668537740752 29.93443815803151 29.190166466194444 21.094726838033292 86-87 19.60120877325849 29.906891789548716 29.1031466714186 21.388752765774193 88-89 19.677685214059785 29.556956386154056 29.21318364740648 21.55217475237968 90-91 19.459918231082586 29.515519906855314 29.369387297452633 21.655174564609474 92-93 19.54831892996018 29.48378775919946 29.455424041295196 21.51246926954516 94-95 19.172717957856282 29.40467174529633 29.517530182206976 21.905080114640413 96-97 19.177395643070344 29.367398761527152 29.275924028452206 22.179281566950298 98-99 19.186073707775066 29.519566678175384 28.99484335148741 22.29951626256214 100-101 18.86247154099656 29.78991203513248 29.26729111871173 22.080325305159228 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1427.0 1 976.0 2 633.0 3 787.0 4 741.5 5 722.0 6 1122.5 7 2169.0 8 3346.5 9 3812.0 10 3408.5 11 2941.5 12 2844.5 13 2811.0 14 2885.5 15 2994.5 16 3018.0 17 2992.5 18 3077.0 19 3217.0 20 3345.5 21 3646.5 22 4121.5 23 4559.0 24 5068.5 25 5789.5 26 6611.5 27 7598.0 28 8670.5 29 9989.5 30 11266.5 31 12347.0 32 13695.5 33 15242.5 34 16952.5 35 17992.0 36 19574.5 37 21329.5 38 21655.5 39 21475.0 40 21194.0 41 21163.5 42 20895.5 43 21041.0 44 22033.0 45 22424.5 46 21940.5 47 22431.5 48 25298.5 49 24385.5 50 20992.5 51 18478.5 52 15728.0 53 14699.5 54 13800.0 55 12725.5 56 11796.5 57 10959.5 58 10274.5 59 9609.0 60 8534.0 61 7252.0 62 5915.0 63 4072.5 64 2530.0 65 1813.5 66 1318.0 67 940.5 68 656.0 69 459.0 70 365.5 71 308.0 72 268.0 73 254.5 74 205.0 75 176.0 76 134.5 77 76.0 78 46.5 79 26.5 80 17.5 81 12.5 82 7.5 83 3.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04707384802720482 2 0.0 3 0.0 4 1.4849794330348525E-4 5 0.010543353974547453 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0057914197888359244 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014849794330348525 24-25 5.93991773213941E-4 26-27 0.002598714007810992 28-29 0.0035639506392836457 30-31 0.0013364814897313673 32-33 0.0 34-35 0.0 36-37 2.969958866069705E-4 38-39 5.197428015621984E-4 40-41 7.424897165174262E-5 42-43 0.01217683135088579 44-45 0.0 46-47 0.0 48-49 7.424897165174262E-5 50-51 0.0 52-53 2.2274691495522786E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.454938299104557E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007276399221870777 92-93 0.002153220177900536 94-95 0.0 96-97 0.0 98-99 0.0011137345747761393 100-101 0.010840349861154423 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 673410.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.552708808844976 #Duplication Level Percentage of deduplicated Percentage of total 1 78.75184670535286 44.53630254886618 2 12.251417462281314 13.857016884799872 3 3.840461987308389 6.515655853790683 4 1.7161035693238542 3.8820122176716576 5 0.8688452245804508 2.4567775492826875 6 0.57044259348713 1.9356044328983995 7 0.3506831043952176 1.3882455640931166 8 0.25648248534892837 1.1603823446805435 9 0.2114628015036783 1.0762914813606095 >10 1.0644919594343014 10.898478086894574 >50 0.07261120827130081 2.8144239234785737 >100 0.04092625321911643 4.213716572995336 >500 0.0023763630901290087 0.8996648482304679 >1k 0.0010561613733906705 1.0153893722767104 >5k 7.921210300430028E-4 3.3500383186805887 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9460 1.4047905436509704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7516 1.1161105418689952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5459 0.8106502724937259 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2285 0.3393178004484638 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1768 0.2625443637605619 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1458 0.2165100013364815 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1289 0.1914138489181925 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 893 0.13260866337001231 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 786 0.1167193834365394 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 744 0.11048246981779303 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 708 0.10513654385886756 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.4849794330348525E-4 0.0 16-17 0.0 0.0 0.0 1.4849794330348525E-4 0.0 18-19 0.0 0.0 0.0 2.969958866069705E-4 0.0 20-21 0.0 0.0 0.0 2.969958866069705E-4 0.0 22-23 0.0 0.0 0.0 5.93991773213941E-4 0.0 24-25 0.0 0.0 0.0 0.0012622325180796246 0.0 26-27 0.0 0.0 0.0 0.0020789712062487934 0.0 28-29 0.0 0.0 0.0 0.003341203724328418 0.0 30-31 0.0 0.0 0.0 0.005345925958925469 0.0 32-33 0.0 0.0 0.0 0.007796142023432976 0.0 34-35 0.0 0.0 0.0 0.009726615286378283 0.0 36-37 0.0 0.0 0.0 0.012548076209144502 0.0 38-39 0.0 0.0 0.0 0.01588927993347292 0.0 40-41 0.0 0.0 0.0 0.02413091578681635 0.0 42-43 0.0 0.0 0.0 0.0337832821015429 0.0 44-45 0.0 0.0 0.0 0.05412750033412037 0.0 46-47 0.0 0.0 0.0 0.09852838538186245 0.0 48-49 0.0 0.0 0.0 0.13030694524880831 0.0 50-51 0.0 0.0 0.0 0.17077263479900803 0.0 52-53 0.0 0.0 0.0 0.2193314622592477 0.0 54-55 0.0 0.0 0.0 0.2687070284076566 0.0 56-57 0.0 0.0 0.0 0.3236512674299461 0.0 58-59 0.0 0.0 0.0 0.4175019675977488 0.0 60-61 0.0 0.0 0.0 0.5423144889443281 0.0 62-63 0.0 0.0 0.0 0.7093004261890973 0.0 64-65 0.0 0.0 0.0 0.8392361265796469 0.0 66-67 0.0 0.0 0.0 1.031021220356098 0.0 68-69 0.0 0.0 0.0 1.256366849319137 0.0 70-71 0.0 0.0 0.0 1.436346356602961 0.0 72-73 0.0 0.0 0.0 1.7718032105255341 0.0 74-75 0.0 0.0 0.0 2.03835701875529 0.0 76-77 0.0 0.0 0.0 2.2763992218707774 0.0 78-79 0.0 0.0 0.0 2.5797805200397974 0.0 80-81 0.0 0.0 0.0 2.866678546502131 0.0 82-83 0.0 0.0 0.0 3.252624701147889 0.0 84-85 0.0 0.0 0.0 3.7209872143270815 0.0 86-87 0.0 0.0 0.0 4.310821045128526 0.0 88-89 0.0 0.0 0.0 5.057765699945056 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5970 0.0 33.910378 1 GTATCAA 9050 0.0 31.716534 1 ATGGGCG 100 4.9518203E-5 28.498552 5 GTCTAGG 130 9.976657E-6 25.588923 1 GTACATG 7595 0.0 25.466188 1 GTCTAGC 95 0.0012226788 25.011728 1 ATCAACG 11490 0.0 24.885592 3 TATCAAC 11615 0.0 24.617775 2 TCAACGC 11640 0.0 24.564903 4 TACATGG 7720 0.0 24.548626 2 CAACGCA 11760 0.0 24.35463 5 AACGCAG 12030 0.0 23.8475 6 CTGGTCG 100 0.0016527118 23.750557 9 ACATGGG 7695 0.0 23.517324 3 AGTACTT 6865 0.0 23.387293 12-13 GAGTACT 6805 0.0 23.279387 12-13 GTATAGC 210 3.423702E-8 22.629662 1 ATGGGAG 1890 0.0 22.617899 5 GTACTTT 7265 0.0 21.607643 14-15 CATGGGA 6040 0.0 21.46828 4 >>END_MODULE