FastQCFastQC Report
Thu 26 May 2016
SRR937461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721690
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110081.5253086505286202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81841.1340049051531822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56710.7857944546827585No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18660.2585597694300877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18160.25163158696947446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.18609098089207277No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10920.1513115049397941No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10070.1395335947567515No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.12581579348473723No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.12318308414970416No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.10780251908714268No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7720.10697113719186908No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.10129002757416619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG405.364856E-447.53942
GGTATCA57800.038.33561
GTATCAA96200.037.5649451
GTACACG1051.8850405E-631.6995411
GTACGTG600.003929968531.6995371
TATCAAC121550.029.8025672
ATCAACG122800.029.4992053
TCAACGC123900.029.2393364
CAACGCA125300.028.9066145
AACGCAG126350.028.6880686
ACGACTA1004.943698E-528.5058148
GCCGTTC856.397204E-427.9468758
CTGTGCG700.00838402727.1465059
TCGACCG350.00839670927.13991218-19
ACGCAGA138550.026.0608887
CGCAGAG138900.025.9268138
GTACATG76250.025.8169351
GAGTACT76150.024.89172412-13
TACATGG77350.024.8913462
GCAGAGT142750.024.8264549
Produced by FastQC (version 0.11.5)