FastQCFastQC Report
Thu 26 May 2016
SRR937461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721690
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97801.355152489295958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79771.105322229766243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59160.8197425487397636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24470.339065249622414No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18070.25038451412656404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.2203162022475024No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13540.18761518103340769No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10640.14743172276185065No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.128309939170558No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.11722484723357673No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.10503124610289738No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGC250.00502179656.996349
GGACCGA1054.656067E-836.1881526
GGTATCA68950.031.9163681
GTATCAA101400.030.5147481
AGGACCG1405.7937905E-727.1467595
TCGCGGT350.00838638827.14675938-39
TACCTGG6050.025.9128152
GTACATG85900.024.9545731
ATGGGAG20850.024.8357035
CCCTATA1151.2798578E-424.786172
TATCAAC126900.024.3711152
ATCAACG126150.024.3653723
TCAACGC127500.024.1073864
TACATGG87300.024.0527112
CAACGCA129450.023.8176365
GAGTACT71850.023.69889612-13
AACGCAG131950.023.3975126
AGTACTT71750.023.3347412-13
ACATGGG87450.023.0879383
GTACTTT76400.022.16317214-15
Produced by FastQC (version 0.11.5)