##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937461_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 721690 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.189671465587715 33.0 31.0 34.0 31.0 34.0 2 32.373397164987736 34.0 31.0 34.0 31.0 34.0 3 32.42307084759384 34.0 31.0 34.0 31.0 34.0 4 36.057325167315604 37.0 35.0 37.0 35.0 37.0 5 35.98659812384819 37.0 35.0 37.0 35.0 37.0 6 36.09581953470327 37.0 35.0 37.0 35.0 37.0 7 36.04744973603625 37.0 35.0 37.0 35.0 37.0 8 36.039830120966066 37.0 35.0 37.0 35.0 37.0 9 37.71182225055079 39.0 37.0 39.0 35.0 39.0 10-11 37.649216422563704 39.0 37.0 39.0 35.0 39.0 12-13 37.57252559963419 39.0 37.0 39.0 35.0 39.0 14-15 38.673078468594554 40.0 38.0 41.0 34.0 41.0 16-17 38.67704554587149 40.0 38.0 41.0 34.5 41.0 18-19 38.79278083387604 40.0 38.0 41.0 35.0 41.0 20-21 38.763038146572626 40.0 38.0 41.0 35.0 41.0 22-23 38.63865302276601 40.0 38.0 41.0 34.0 41.0 24-25 38.49485790297773 40.0 38.0 41.0 34.0 41.0 26-27 38.37945586054954 40.0 38.0 41.0 34.0 41.0 28-29 38.25557510842606 40.0 38.0 41.0 34.0 41.0 30-31 38.15788635009491 40.0 37.0 41.0 34.0 41.0 32-33 37.91934140697529 40.0 36.5 41.0 33.5 41.0 34-35 37.74672643378736 40.0 36.0 41.0 33.0 41.0 36-37 37.53066344275243 40.0 36.0 41.0 33.0 41.0 38-39 37.343932297801 40.0 35.0 41.0 33.0 41.0 40-41 37.096188114010175 40.0 35.0 41.0 33.0 41.0 42-43 36.75028613393562 40.0 35.0 41.0 31.5 41.0 44-45 36.21804029430919 39.5 35.0 41.0 30.5 41.0 46-47 35.53459657193532 39.0 35.0 41.0 27.5 41.0 48-49 34.97703099668833 39.0 35.0 41.0 23.5 41.0 50-51 34.6721272291427 39.0 35.0 41.0 22.5 41.0 52-53 34.408400421233495 39.0 35.0 40.5 20.0 41.0 54-55 34.178306475079324 38.5 34.5 40.0 18.5 41.0 56-57 33.92045268744198 38.0 34.0 40.0 16.0 41.0 58-59 33.674148179966465 38.0 34.0 40.0 12.5 41.0 60-61 33.34767213069323 38.0 34.0 40.0 8.0 41.0 62-63 32.9962005847386 37.0 33.0 40.0 6.5 41.0 64-65 32.654817858083106 36.5 33.0 40.0 2.0 41.0 66-67 32.23531086754701 36.0 32.5 39.0 2.0 41.0 68-69 31.840098934445535 35.5 32.0 39.0 2.0 40.0 70-71 31.45134960994333 35.0 31.5 38.5 2.0 40.0 72-73 31.012881569649018 35.0 31.0 38.0 2.0 40.0 74-75 30.5636408984467 35.0 31.0 37.0 2.0 39.0 76-77 29.253138466654658 33.5 29.5 35.5 2.0 38.5 78-79 29.81838878188696 34.5 30.0 36.0 2.0 39.0 80-81 29.649320345300616 34.5 30.0 36.0 2.0 37.5 82-83 29.306477157782428 34.0 30.0 35.5 2.0 37.0 84-85 28.933783896132688 34.0 29.5 35.0 2.0 37.0 86-87 28.549064695367818 34.0 29.0 35.0 2.0 36.0 88-89 28.142024276351343 34.0 29.0 35.0 2.0 36.0 90-91 27.696209591375798 34.0 27.5 35.0 2.0 35.5 92-93 27.350037412185287 34.0 27.0 35.0 2.0 35.0 94-95 26.956035139741438 33.5 26.0 35.0 2.0 35.0 96-97 26.514116864581744 33.0 25.0 35.0 2.0 35.0 98-99 26.098856849894 33.0 24.5 35.0 2.0 35.0 100-101 24.403766852803834 31.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14005603875335737 1101 2 0.09757795076762221 1101 3 0.08045059039595515 1101 4 0.1539342546776865 1101 5 0.1328558630124732 1101 6 0.06476291383300037 1101 7 0.10333339983587564 1101 8 -0.01619989217839901 1101 9 0.26159031616797535 1101 10-11 0.21758705966971092 1101 12-13 0.15787497035414333 1101 14-15 0.15475397958797998 1101 16-17 0.1656520511547157 1101 18-19 0.17928928070442396 1101 20-21 0.036931087142626495 1101 22-23 0.2305675048714093 1101 24-25 0.20227842106334037 1101 26-27 0.22871190138618402 1101 28-29 0.13916646176374314 1101 30-31 0.10407011589388304 1101 32-33 0.40301935282253254 1101 34-35 0.2590988744039109 1101 36-37 0.11497474873607416 1101 38-39 0.14871397534083286 1101 40-41 0.36052069840045675 1101 42-43 0.534890793544605 1101 44-45 0.19400340987968434 1101 46-47 0.06100024207826493 1101 48-49 0.04535893935356938 1101 50-51 0.07919541955352827 1101 52-53 0.17056397672004664 1101 54-55 0.13910558429778774 1101 56-57 0.21721442899594479 1101 58-59 0.0595479485108541 1101 60-61 0.051756536073703785 1101 62-63 0.2162412876164126 1101 64-65 0.14746509687850562 1101 66-67 0.14141725403685967 1101 68-69 0.23552324787975465 1101 70-71 0.1286020789095197 1101 72-73 -0.019878105425558346 1101 74-75 -0.22785881892554016 1101 76-77 -0.08465332050035457 1101 78-79 -0.056993993627202144 1101 80-81 0.11704808646251408 1101 82-83 -0.012527554444744737 1101 84-85 0.05150072334084399 1101 86-87 0.07041634745188574 1101 88-89 0.0796919399130438 1101 90-91 -0.13525862205087336 1101 92-93 -0.1267957345507753 1101 94-95 -0.15890978901659025 1101 96-97 -0.3000621000736494 1101 98-99 -0.16037660412145271 1101 100-101 0.3499731386798466 1102 1 0.18672787804631952 1102 2 0.15773852444421266 1102 3 0.09427271670906379 1102 4 0.12972242350735996 1102 5 -0.06419846774868887 1102 6 -0.027576042710876436 1102 7 0.12456591949084839 1102 8 0.06218964295201346 1102 9 0.028284159328286762 1102 10-11 0.02721572167697417 1102 12-13 0.1933615104127 1102 14-15 0.1641685130514432 1102 16-17 0.004862427523903534 1102 18-19 0.11121216412030321 1102 20-21 0.06516786815262066 1102 22-23 0.2231161952473002 1102 24-25 0.1949074285858785 1102 26-27 0.07397106814882903 1102 28-29 0.23768074609250078 1102 30-31 0.3264834491463944 1102 32-33 0.24959238687093688 1102 34-35 0.28913066787064423 1102 36-37 0.0687681427265332 1102 38-39 0.11758805017822738 1102 40-41 0.03932788269344911 1102 42-43 -0.022052548012396755 1102 44-45 0.20291558232212026 1102 46-47 0.3430642108970261 1102 48-49 0.11323905161572867 1102 50-51 0.4206524029021139 1102 52-53 0.4143704639208323 1102 54-55 0.3076617244678772 1102 56-57 0.3544499128082492 1102 58-59 0.3358313558976036 1102 60-61 0.16350875313003144 1102 62-63 0.07127338416671591 1102 64-65 0.13823241355840565 1102 66-67 0.12710034379202995 1102 68-69 0.3112911101100835 1102 70-71 0.13301194488596835 1102 72-73 0.18472679482963628 1102 74-75 0.06635046634277941 1102 76-77 0.1213745571266216 1102 78-79 0.21502351247993445 1102 80-81 0.19607637022147983 1102 82-83 0.024787337263497022 1102 84-85 0.058372385633003177 1102 86-87 0.01809048411833203 1102 88-89 0.04822353031378057 1102 90-91 0.21649411024753107 1102 92-93 -0.06512732010217093 1102 94-95 -0.02290785534340145 1102 96-97 -0.16946717732965055 1102 98-99 -0.1129167475406021 1102 100-101 0.27403782277583844 1103 1 0.08966805972394098 1103 2 0.10556660716550681 1103 3 0.024615536577641706 1103 4 -0.04391620231405824 1103 5 -0.03936275070191897 1103 6 -0.0523151227263412 1103 7 0.0764648371560952 1103 8 0.029429689337789 1103 9 0.021392731032968015 1103 10-11 -0.033064646510119644 1103 12-13 -0.005121892177179177 1103 14-15 -0.0734468474046821 1103 16-17 -0.08003320448228379 1103 18-19 0.07916792851921883 1103 20-21 -0.026549734282632187 1103 22-23 -0.010971913565995806 1103 24-25 -0.08619761586715668 1103 26-27 -0.09431326215653968 1103 28-29 -0.4337593872665977 1103 30-31 -0.22673637594057539 1103 32-33 -0.3120016409605242 1103 34-35 -0.22816132091946173 1103 36-37 -0.22803437949998084 1103 38-39 -0.013460824000354421 1103 40-41 -0.12061244393971293 1103 42-43 -0.023257490996542174 1103 44-45 0.024248810965332268 1103 46-47 0.016144153881178624 1103 48-49 -0.1417075564244712 1103 50-51 -0.25043742121848567 1103 52-53 -0.1457679686265294 1103 54-55 -0.1360441848865861 1103 56-57 -0.4274795663181479 1103 58-59 -0.3391541485476992 1103 60-61 -0.6306030317559319 1103 62-63 -0.6360976053964791 1103 64-65 -0.48585557445860417 1103 66-67 -0.23064390630460707 1103 68-69 -0.3686410023754192 1103 70-71 -0.37642526520293984 1103 72-73 -0.40436952813132265 1103 74-75 -0.5823376859571816 1103 76-77 -0.5022121262904626 1103 78-79 -0.3263914952510305 1103 80-81 -0.5051007896161721 1103 82-83 -0.623345152106431 1103 84-85 -0.3533255270071187 1103 86-87 -0.28681142929488956 1103 88-89 -0.3752852394968116 1103 90-91 -0.4316413990296226 1103 92-93 -0.13112135276548997 1103 94-95 -0.15205418796806214 1103 96-97 -0.1402012563787416 1103 98-99 0.050967577932922836 1103 100-101 0.0032669008585592962 1104 1 0.13617800985783646 1104 2 0.11581974688293428 1104 3 0.006093223806374226 1104 4 -0.07033398096398003 1104 5 -0.02246368338268212 1104 6 0.02809396398527042 1104 7 0.0787561382919364 1104 8 0.06417435643540159 1104 9 0.06496063896537407 1104 10-11 0.12888402719013214 1104 12-13 0.02003841232924941 1104 14-15 -0.050709146555107054 1104 16-17 -0.15993439572800838 1104 18-19 -0.08869945882032937 1104 20-21 0.004412410190539617 1104 22-23 -0.13951780307564832 1104 24-25 -0.064447569694849 1104 26-27 -0.1450487005626755 1104 28-29 0.014837840085881737 1104 30-31 -0.05136165874601062 1104 32-33 -0.21486374734153202 1104 34-35 -0.017452518167594633 1104 36-37 -0.14014104909090008 1104 38-39 -0.2041142488318286 1104 40-41 -0.17097633949793334 1104 42-43 -0.17419983908963133 1104 44-45 -0.4928486530202605 1104 46-47 -0.5001366372814431 1104 48-49 -0.3786335768326339 1104 50-51 -0.5384588798019152 1104 52-53 -0.44918500851940735 1104 54-55 -0.28074172736511827 1104 56-57 -0.3434264739273516 1104 58-59 -0.3041497621777651 1104 60-61 -0.2629263937930588 1104 62-63 -0.5050807755876363 1104 64-65 -0.5226794386196474 1104 66-67 -0.30084746849871635 1104 68-69 -0.11047860280020672 1104 70-71 -0.29906138695933393 1104 72-73 -0.29042130461114724 1104 74-75 -0.352991139646349 1104 76-77 -0.14533187418962257 1104 78-79 -0.1006334035528127 1104 80-81 -0.01660996059225539 1104 82-83 -0.05686567570048595 1104 84-85 -0.14988453522579093 1104 86-87 -0.1316060779235677 1104 88-89 -0.18107798928903662 1104 90-91 -0.24464713016823225 1104 92-93 -0.11634981915204534 1104 94-95 0.009083584448596582 1104 96-97 0.13431840725594313 1104 98-99 0.23872441143555534 1104 100-101 0.8198257359310546 1105 1 0.00473631400851815 1105 2 0.11922991924203075 1105 3 0.06832692557723874 1105 4 0.13530734292309177 1105 5 0.0323789790709057 1105 6 0.04892042928641871 1105 7 0.06154355377626075 1105 8 0.08184100858849064 1105 9 0.05309734442475644 1105 10-11 -0.015182148502944415 1105 12-13 -0.039463885999417414 1105 14-15 0.2006071830855518 1105 16-17 -0.02114520129376274 1105 18-19 0.18281044047617456 1105 20-21 0.2416005496950504 1105 22-23 0.1926855445846627 1105 24-25 0.0037411047386584073 1105 26-27 0.17739297040623825 1105 28-29 -0.05408247415380174 1105 30-31 -0.06705809276002839 1105 32-33 0.011247163343981015 1105 34-35 -0.08920531254958775 1105 36-37 -0.10356372386029022 1105 38-39 -0.11079479669864156 1105 40-41 -0.19161309160811157 1105 42-43 -0.46160132818268096 1105 44-45 -0.3876278082056501 1105 46-47 -0.20119529908812694 1105 48-49 -0.0813934372534888 1105 50-51 -0.12179428650345869 1105 52-53 0.0955321011223802 1105 54-55 -0.06935613685948283 1105 56-57 -0.09001008146362466 1105 58-59 -0.03347957700525228 1105 60-61 0.08898457784948732 1105 62-63 -0.045710498979580905 1105 64-65 0.1727671337916945 1105 66-67 0.1473017393709597 1105 68-69 0.2020109007732458 1105 70-71 0.3610790941035553 1105 72-73 0.30918978334735314 1105 74-75 0.2379467899296266 1105 76-77 0.2945819151603146 1105 78-79 0.3518605473954466 1105 80-81 0.33910693756597254 1105 82-83 0.21260791684534297 1105 84-85 0.23219161129499 1105 86-87 0.45779548581073115 1105 88-89 0.47448371725886673 1105 90-91 0.30155347442593694 1105 92-93 0.15413609542103757 1105 94-95 0.059518525264302014 1105 96-97 0.2918029036451735 1105 98-99 0.27818708769776634 1105 100-101 0.6527350654659756 1106 1 -0.1017351811696443 1106 2 0.0355593165305379 1106 3 0.023282932307800763 1106 4 0.10887246556313102 1106 5 -0.045415353321040186 1106 6 -0.22891356005438723 1106 7 -0.10071822656130536 1106 8 -0.03398507901260217 1106 9 -0.24304926027329543 1106 10-11 -0.15628583474606472 1106 12-13 -0.3391490394755081 1106 14-15 -0.2860178623161005 1106 16-17 -0.1599000472246601 1106 18-19 -0.10844297854581697 1106 20-21 -0.02692888169102048 1106 22-23 -0.14263063619172556 1106 24-25 -0.06139914002254443 1106 26-27 0.15236554410515168 1106 28-29 0.01311011036738563 1106 30-31 0.13722708147111007 1106 32-33 0.14535158342596333 1106 34-35 0.04477494792614323 1106 36-37 0.21600827759256447 1106 38-39 0.1300331440308966 1106 40-41 -0.15385189315287562 1106 42-43 -0.18813330947349272 1106 44-45 0.07462211210982161 1106 46-47 0.27184168194324343 1106 48-49 -0.006805017082548659 1106 50-51 -0.05857851563908412 1106 52-53 -0.03093187035426581 1106 54-55 -0.23991252014575082 1106 56-57 -0.14658979934335292 1106 58-59 -0.05818270582732765 1106 60-61 -0.2050337088220715 1106 62-63 -0.08348466933397702 1106 64-65 -0.13581396281560387 1106 66-67 -0.5013413999768837 1106 68-69 -0.4023378357637739 1106 70-71 -0.32573385852306913 1106 72-73 -0.4726237993429443 1106 74-75 -0.5180666118953354 1106 76-77 -0.17375182586127735 1106 78-79 0.03052807391371104 1106 80-81 0.0071875412410982165 1106 82-83 -0.02777249336316956 1106 84-85 -0.20171470935552804 1106 86-87 -0.08659064685844342 1106 88-89 -0.11924381355865776 1106 90-91 -0.28272588098186446 1106 92-93 -0.1976784879586262 1106 94-95 -0.40569176775236926 1106 96-97 -0.13127280777084138 1106 98-99 -0.08192487832327089 1106 100-101 -0.0349831404091816 1107 1 0.19854209970930725 1107 2 0.22963325960973435 1107 3 0.2563847825852008 1107 4 0.10062885742019034 1107 5 0.06608647519104949 1107 6 0.11545886240105574 1107 7 0.19748001453022823 1107 8 0.07157890304009129 1107 9 0.3388166155873975 1107 10-11 0.26003791505272744 1107 12-13 0.27512333060717964 1107 14-15 0.25057618532724746 1107 16-17 0.3171080852897532 1107 18-19 0.22582444899953202 1107 20-21 0.35207984483476196 1107 22-23 0.3675129014407048 1107 24-25 0.3271534447204303 1107 26-27 0.46557050750089957 1107 28-29 0.5781588895833352 1107 30-31 0.4697532848542494 1107 32-33 0.4873819686944856 1107 34-35 0.5830796122757107 1107 36-37 0.3706280763088685 1107 38-39 0.47334564162198234 1107 40-41 0.5055018587544211 1107 42-43 0.937487625119509 1107 44-45 1.0174020399669104 1107 46-47 0.7664479680345408 1107 48-49 0.7707082518164157 1107 50-51 0.38870184185599754 1107 52-53 0.4937332047246912 1107 54-55 0.34358121892908855 1107 56-57 0.14421390710620585 1107 58-59 0.09525247098174106 1107 60-61 0.47050231842082724 1107 62-63 0.43673779019049874 1107 64-65 0.21704466021164137 1107 66-67 0.14715804115363795 1107 68-69 0.34387080732835074 1107 70-71 0.1596029028453323 1107 72-73 0.01767023628413611 1107 74-75 -0.058059220761521146 1107 76-77 -0.016524725339589708 1107 78-79 0.2895283663849568 1107 80-81 0.17321145133226068 1107 82-83 0.14550084935645202 1107 84-85 0.2017686458564647 1107 86-87 0.4572494325849128 1107 88-89 0.3787519648796369 1107 90-91 0.32824541829330656 1107 92-93 0.2971794594373627 1107 94-95 0.18506047282676974 1107 96-97 0.21296698237537015 1107 98-99 0.33592811066484884 1107 100-101 0.7910712864491991 1108 1 0.04989838172751604 1108 2 0.047594782478569186 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50-51 -0.7234146256273064 2316 52-53 -0.44226239979656157 2316 54-55 -0.5608806366362415 2316 56-57 -0.6567445867436135 2316 58-59 -0.6744882783457555 2316 60-61 -0.6595708231078703 2316 62-63 -0.9229260700180362 2316 64-65 -0.7763668855423091 2316 66-67 -0.5939787490670554 2316 68-69 -0.48970283758429645 2316 70-71 -0.2800100790614266 2316 72-73 -0.18790414544286804 2316 74-75 -0.2571054907976347 2316 76-77 -0.37426873951691775 2316 78-79 -0.5775163410965654 2316 80-81 -0.6573704732141366 2316 82-83 -0.7088671576559946 2316 84-85 -0.7426907960066593 2316 86-87 -0.8314150596324872 2316 88-89 -0.5194834917705897 2316 90-91 -0.793671950517215 2316 92-93 -0.7172154158917436 2316 94-95 -0.7689791436486537 2316 96-97 -0.7048004066022635 2316 98-99 -0.8451853047555318 2316 100-101 -1.4956082532959023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 5.0 5 16.0 6 37.0 7 110.0 8 240.0 9 447.0 10 920.0 11 1537.0 12 2524.0 13 3692.0 14 4684.0 15 6967.0 16 10557.0 17 15572.0 18 13115.0 19 6156.0 20 4521.0 21 4345.0 22 4404.0 23 4697.0 24 5121.0 25 5773.0 26 6635.0 27 8081.0 28 9719.0 29 12028.0 30 14514.0 31 18231.0 32 23256.0 33 30330.0 34 42331.0 35 62537.0 36 92575.0 37 145588.0 38 142394.0 39 18027.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.26114974655886 20.530812231723957 15.841596512827339 18.36644150888985 2 15.269159888594826 22.804389696407046 40.47970735357286 21.446743061425266 3 19.199656362149952 29.54190857570425 30.87502944477546 20.38340561737034 4 11.98215300198146 18.381438013551524 38.10278651498566 31.533622469481354 5 11.620548867866477 41.01097902039753 32.45560301458788 14.912869097148116 6 27.50530005958237 38.66313791240006 18.02519087142679 15.806371156590778 7 25.89394338289293 37.11995455112306 20.291260790644184 16.69484127533983 8 27.52857875265003 33.69258268785767 19.47706078787291 19.301777771619395 9 28.922113372777787 16.196982083720158 20.610095747481605 34.270808796020454 10-11 24.412490127339996 29.180465296734052 25.710900802283533 20.696143773642422 12-13 26.664107788803893 27.252187805930824 27.5014705464613 18.582233858803985 14-15 23.475314885892836 26.868669373276617 28.673460904266374 20.982554836564177 16-17 19.441380648200752 30.71214787512644 28.823594618187865 21.022876858484945 18-19 19.493965553076805 27.241613435131427 34.51973839183029 18.744682619961477 20-21 21.448544388865027 25.71013870221286 34.659826241183886 18.181490667738228 22-23 20.529126849245404 26.365304820214515 34.492458217255724 18.61311011328436 24-25 19.83174192761702 26.460774655010677 35.04023201129572 18.66725140607658 26-27 19.711436202458856 26.74130757852365 34.964786902737025 18.582469316280473 28-29 19.31763305524936 26.92490762674345 35.00068245008588 18.756776867921314 30-31 19.481217697349276 26.69283210242625 34.99840651803406 18.827543682190413 32-33 19.292286161648352 26.976610456012967 34.63204422951683 19.099059152821848 34-35 19.575648824287438 26.882040765425597 34.71587523729025 18.826435172996717 36-37 19.774210533608613 26.74029015228145 34.45904751347532 19.02645180063462 38-39 19.727237456525657 27.028363978993752 34.302609153514666 18.941789410965928 40-41 19.641951530435506 27.316714933004477 34.127603264559575 18.913730272000443 42-43 19.5953132784669 27.530158175812343 33.52486001021383 19.349668535506925 44-45 19.82194571076224 27.66236195596447 33.02373595311006 19.491956380163227 46-47 20.19502833626626 28.235253363632584 32.03764774349097 19.53207055661018 48-49 20.196275409109173 28.565866230653054 31.32861755047181 19.909240809765965 50-51 20.275949507406228 28.91927281796893 30.911540966342887 19.89323670828195 52-53 20.297080463911097 29.45696905873713 30.267566406628887 19.97838407072289 54-55 20.012609292078317 29.342030511715556 30.31447020188724 20.33088999431889 56-57 20.454558051240838 29.533802602225336 29.825340520167938 20.186298826365892 58-59 20.424004766589533 29.089706106500017 30.16073383308623 20.32555529382422 60-61 20.332483476284832 29.309468054150674 30.552383987584697 19.805664481979797 62-63 20.449569759869195 29.178456123820478 30.57739472626751 19.794579390042816 64-65 20.27484099821253 29.280231124166882 30.506796546993865 19.938131330626724 66-67 20.308096274023473 29.44914021255664 30.102467818592473 20.14029569482742 68-69 20.53513281325777 29.39260624367803 29.991616899222656 20.080644043841538 70-71 20.319181365960453 29.715459546342615 29.87085867893417 20.094500408762766 72-73 20.319126161597197 29.60691543939603 29.72275473039306 20.351203668613717 74-75 20.17105682495254 30.228699303024843 29.294711025509567 20.305532846513046 76-77 19.94790006789619 29.74732918566143 29.829358865995093 20.475411880447282 78-79 20.07621000706675 29.697792681068048 29.56373235045518 20.662264961410024 80-81 19.935082930344052 30.141889176793363 29.418379082431517 20.50464881043107 82-83 19.867781833096387 30.053444295718634 29.33956041824284 20.73921345294214 84-85 19.795920346857816 29.817966159469794 29.21409657202163 21.17201692165076 86-87 19.641812966786294 29.765203896409815 29.109243581038953 21.48373955576494 88-89 19.69238869874877 29.5537557677119 29.145547257132563 21.608308276406767 90-91 19.56531831318506 29.41137749901782 29.30758160415239 21.715722583644734 92-93 19.626025126112076 29.37162549303396 29.422686940671035 21.579662440182933 94-95 19.21669969100306 29.274550014549185 29.527082265238537 21.981668029209217 96-97 19.2374842383849 29.264642713630508 29.30468760825285 22.193185439731742 98-99 19.182263791460986 29.355434311942496 29.126179960162812 22.336121936433706 100-101 18.949053302614026 29.49531415109048 29.33670552774265 22.218927018552844 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1628.0 1 1119.0 2 771.0 3 935.5 4 811.0 5 770.0 6 1258.5 7 2401.0 8 3503.5 9 3935.0 10 3563.5 11 3000.5 12 2902.5 13 2979.5 14 3119.0 15 3185.0 16 3097.5 17 3143.0 18 3290.5 19 3374.5 20 3555.0 21 3891.0 22 4224.5 23 4737.0 24 5454.0 25 6207.0 26 7143.0 27 8291.5 28 9254.0 29 10469.0 30 11955.0 31 13056.5 32 14475.0 33 16177.5 34 17954.5 35 19099.5 36 20832.0 37 22815.5 38 23185.0 39 23068.5 40 22768.0 41 22611.5 42 22374.5 43 22465.0 44 23504.0 45 24148.5 46 23725.0 47 24129.0 48 26921.5 49 26101.5 50 22578.0 51 19884.0 52 17111.0 53 15906.5 54 14959.0 55 13876.5 56 12828.0 57 11889.0 58 11174.0 59 10375.5 60 9252.5 61 7896.5 62 6428.5 63 4392.0 64 2691.0 65 1982.5 66 1459.0 67 1048.5 68 749.5 69 530.0 70 392.0 71 312.5 72 300.0 73 319.0 74 233.5 75 181.5 76 142.0 77 83.0 78 62.0 79 40.0 80 20.0 81 12.0 82 7.0 83 2.5 84 0.5 85 2.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057919605370727045 2 0.0 3 0.0 4 0.0 5 0.018290401696019067 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0027019911596391803 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014549183167287894 24-25 1.3856364921226565E-4 26-27 0.0017320456151533206 28-29 0.003533373054912774 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.016558356080865744 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.928182460613282E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.542545968490626E-4 84-85 0.0015242001413349221 86-87 0.0 88-89 0.0 90-91 0.01129293741079965 92-93 0.0014549183167287894 94-95 0.0 96-97 0.0 98-99 3.4640912303066416E-4 100-101 0.01420277404425723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 721690.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.78282600536267 #Duplication Level Percentage of deduplicated Percentage of total 1 78.35440252259464 43.708300026720465 2 12.306148002284624 13.729434256153692 3 3.959321688802067 6.625864585971132 4 1.7618465112622796 3.93123109543596 5 0.9558192381174184 2.6659149126241135 6 0.5908097067170146 1.9774221043244746 7 0.3714722172532846 1.4505239042606382 8 0.28351167968357294 1.265206615862149 9 0.2072711086654204 1.040595137155959 >10 1.0868453720011466 11.026328735991635 >50 0.0755321328935224 2.9298422641849364 >100 0.042768027516016854 4.342989627328786 >500 0.0022509488165476984 0.8509073126828989 >1k 0.001250527120304277 1.1526801471365982 >5k 7.503162721825662E-4 3.3027592741666285 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9780 1.355152489295958 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7977 1.105322229766243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5916 0.8197425487397636 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2447 0.339065249622414 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1807 0.25038451412656404 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1590 0.2203162022475024 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1354 0.18761518103340769 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1064 0.14743172276185065 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 926 0.128309939170558 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 846 0.11722484723357673 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 0.10503124610289738 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.928182460613282E-5 0.0 22-23 0.0 0.0 0.0 1.3856364921226565E-4 0.0 24-25 0.0 0.0 0.0 2.771272984245313E-4 0.0 26-27 0.0 0.0 0.0 3.464091230306641E-4 0.0 28-29 0.0 0.0 0.0 0.002078454738183985 0.0 30-31 0.0 0.0 0.0 0.004018345827155705 0.0 32-33 0.0 0.0 0.0 0.006027518740733556 0.0 34-35 0.0 0.0 0.0 0.00734387340825008 0.0 36-37 0.0 0.0 0.0 0.009491609971040198 0.0 38-39 0.0 0.0 0.0 0.013509955798195902 0.0 40-41 0.0 0.0 0.0 0.019468192714323325 0.0 42-43 0.0 0.0 0.0 0.027574166193240865 0.0 44-45 0.0 0.0 0.0 0.048843686347323646 0.0 46-47 0.0 0.0 0.0 0.09429256328894678 0.0 48-49 0.0 0.0 0.0 0.13087336668098493 0.0 50-51 0.0 0.0 0.0 0.17736147099170005 0.0 52-53 0.0 0.0 0.0 0.231054885061453 0.0 54-55 0.0 6.928182460613282E-5 0.0 0.2783743712674417 0.0 56-57 1.3856364921226565E-4 1.3856364921226565E-4 0.0 0.3338691127769541 0.0 58-59 1.3856364921226565E-4 1.3856364921226565E-4 0.0 0.434466322105059 0.0 60-61 1.3856364921226565E-4 1.3856364921226565E-4 0.0 0.5694965982624118 0.0 62-63 1.3856364921226565E-4 1.3856364921226565E-4 0.0 0.731754631489975 0.0 64-65 1.3856364921226565E-4 1.3856364921226565E-4 0.0 0.8605495434327759 0.0 66-67 1.3856364921226565E-4 1.3856364921226565E-4 0.0 1.0509359974504289 0.0 68-69 1.3856364921226565E-4 1.3856364921226565E-4 0.0 1.2849007191453394 0.0 70-71 1.3856364921226565E-4 1.3856364921226565E-4 0.0 1.469328936246865 0.0 72-73 1.3856364921226565E-4 1.3856364921226565E-4 0.0 1.7844919563801631 0.0 74-75 1.3856364921226565E-4 1.3856364921226565E-4 0.0 2.047277917111225 0.0 76-77 1.3856364921226565E-4 1.3856364921226565E-4 0.0 2.281311920630742 0.0 78-79 1.3856364921226565E-4 1.3856364921226565E-4 0.0 2.591694494866217 0.0 80-81 1.3856364921226565E-4 1.3856364921226565E-4 0.0 2.880114730701548 0.0 82-83 1.3856364921226565E-4 1.3856364921226565E-4 0.0 3.2545829926976957 0.0 84-85 1.3856364921226565E-4 2.771272984245313E-4 0.0 3.715930662749934 0.0 86-87 1.3856364921226565E-4 2.771272984245313E-4 0.0 4.294641743684961 0.0 88-89 1.3856364921226565E-4 2.771272984245313E-4 0.0 5.021962338400144 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCGC 25 0.005021796 56.99634 9 GGACCGA 105 4.656067E-8 36.188152 6 GGTATCA 6895 0.0 31.916368 1 GTATCAA 10140 0.0 30.514748 1 AGGACCG 140 5.7937905E-7 27.146759 5 TCGCGGT 35 0.008386388 27.146759 38-39 TACCTGG 605 0.0 25.912815 2 GTACATG 8590 0.0 24.954573 1 ATGGGAG 2085 0.0 24.835703 5 CCCTATA 115 1.2798578E-4 24.78617 2 TATCAAC 12690 0.0 24.371115 2 ATCAACG 12615 0.0 24.365372 3 TCAACGC 12750 0.0 24.107386 4 TACATGG 8730 0.0 24.052711 2 CAACGCA 12945 0.0 23.817636 5 GAGTACT 7185 0.0 23.698896 12-13 AACGCAG 13195 0.0 23.397512 6 AGTACTT 7175 0.0 23.33474 12-13 ACATGGG 8745 0.0 23.087938 3 GTACTTT 7640 0.0 22.163172 14-15 >>END_MODULE