FastQCFastQC Report
Thu 26 May 2016
SRR937460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783174
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110661.4129682548194908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81071.0351467234611977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57700.7367456018713594No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.238005858212862No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18640.238005858212862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.1702048331532967No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11780.15041357348430873No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10960.1399433586916828No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.10674511666628361No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.10176538036247373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.036.8646771
GTATCAA96650.036.0291981
ATCAACG122250.028.3253653
TATCAAC123000.028.1526492
TCAACGC123750.028.0240024
CAACGCA124700.027.7706325
AACGCAG127500.027.2725376
CCGTCGT700.00838964627.1430599
ACAGCGT908.9445745E-426.3890868
GTACATG86650.025.5487611
TACATGG86950.025.074942
ACGCAGA143700.024.09887
CGCAGAG144200.024.0466428
GAGTACT74800.023.93915412-13
ACATGGG88150.023.8175333
ACCGCTC1000.001653100123.7501758
AGTACTT75550.023.73293712-13
GCAGAGT147100.023.0882079
TATATGG2903.6379788E-1222.9312042
CATGGGG17350.022.7264254
Produced by FastQC (version 0.11.5)