##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937460_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 783174 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93203936800762 33.0 31.0 34.0 30.0 34.0 2 31.970037309716616 33.0 31.0 34.0 30.0 34.0 3 31.982809694908156 33.0 31.0 34.0 30.0 34.0 4 35.621273178118784 37.0 35.0 37.0 33.0 37.0 5 35.6691897330606 37.0 35.0 37.0 35.0 37.0 6 35.736201661444326 37.0 35.0 37.0 35.0 37.0 7 35.64656768483121 37.0 35.0 37.0 33.0 37.0 8 35.6602172697255 37.0 35.0 37.0 33.0 37.0 9 37.28459192976273 39.0 37.0 39.0 34.0 39.0 10-11 37.18667499176428 39.0 37.0 39.0 34.0 39.0 12-13 37.042752951451405 39.0 37.0 39.0 33.5 39.0 14-15 38.13146312313739 40.0 38.0 41.0 33.0 41.0 16-17 38.114214721121996 40.0 38.0 41.0 33.0 41.0 18-19 38.216121194013084 40.0 38.0 41.0 33.5 41.0 20-21 38.18642919708775 40.0 38.0 41.0 34.0 41.0 22-23 38.04780674537204 40.0 38.0 41.0 33.5 41.0 24-25 37.969599348292974 40.0 38.0 41.0 33.0 41.0 26-27 37.95477122580678 40.0 38.0 41.0 33.0 41.0 28-29 37.85186561351628 40.0 37.5 41.0 33.0 41.0 30-31 37.520121965233784 39.5 37.0 41.0 33.0 41.0 32-33 37.148286970711496 39.0 35.5 41.0 32.5 41.0 34-35 37.065593980392606 39.0 35.0 41.0 32.0 41.0 36-37 36.885944885810815 39.5 35.0 41.0 31.5 41.0 38-39 36.72087875746641 39.0 35.0 41.0 31.0 41.0 40-41 36.42221651893449 39.0 35.0 41.0 30.5 41.0 42-43 36.011375505315556 39.0 35.0 41.0 30.0 41.0 44-45 35.5341724827433 39.0 35.0 41.0 28.0 41.0 46-47 34.860382239451255 39.0 35.0 41.0 23.5 41.0 48-49 34.29968116919101 39.0 35.0 40.5 18.5 41.0 50-51 33.25556325925018 38.0 33.5 39.5 13.0 40.5 52-53 33.340850181441155 38.0 33.5 39.5 9.5 40.5 54-55 33.68550411530516 38.0 34.0 40.0 8.0 41.0 56-57 33.48831741094571 38.0 34.0 40.0 6.0 41.0 58-59 33.23916396611736 38.0 33.5 40.0 2.0 41.0 60-61 32.93723744659552 38.0 33.0 40.0 2.0 41.0 62-63 32.645129307152686 37.0 33.0 40.0 2.0 41.0 64-65 32.2450796374752 37.0 32.5 40.0 2.0 41.0 66-67 31.89438936941216 36.0 32.0 39.0 2.0 41.0 68-69 31.439909905078565 35.5 31.0 39.0 2.0 40.5 70-71 30.992645312535913 35.0 31.0 39.0 2.0 40.0 72-73 30.5572197238417 35.0 30.5 38.0 2.0 40.0 74-75 30.08620945026265 35.0 30.0 37.0 2.0 39.5 76-77 29.622827749644394 35.0 29.5 37.0 2.0 39.0 78-79 29.150149264403566 34.0 29.0 36.0 2.0 39.0 80-81 28.666914376626394 34.0 29.0 36.0 2.0 37.5 82-83 28.212621333190327 34.0 27.5 35.0 2.0 37.0 84-85 27.70849134419682 34.0 26.5 35.0 2.0 36.5 86-87 27.257118469203526 34.0 26.0 35.0 2.0 36.0 88-89 26.81276829925406 33.5 25.5 35.0 2.0 36.0 90-91 26.32322319178114 33.0 24.5 35.0 2.0 35.0 92-93 25.867893341709504 33.0 23.0 35.0 2.0 35.0 94-95 25.3905422039036 33.0 19.5 35.0 2.0 35.0 96-97 24.7412950378843 32.0 12.0 35.0 2.0 35.0 98-99 23.932827187827993 32.0 2.0 34.0 2.0 35.0 100-101 21.594651380153067 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.017066321378681693 1101 2 0.0901412617315529 1101 3 0.1007450620620034 1101 4 0.06747056487935055 1101 5 0.09739249399177652 1101 6 -0.10442702573390505 1101 7 -0.029773508878527366 1101 8 -0.15706232598869718 1101 9 0.06081744187126503 1101 10-11 -0.037570406812072576 1101 12-13 -0.07703336944205574 1101 14-15 -0.24651897769080477 1101 16-17 -0.09491759799956867 1101 18-19 0.12843518922723973 1101 20-21 0.08804692642894452 1101 22-23 0.20910516893187747 1101 24-25 -0.037529874366832416 1101 26-27 -0.05383657604309633 1101 28-29 -0.08528379828992882 1101 30-31 -0.01404367267312523 1101 32-33 -0.17340343081465193 1101 34-35 0.15546674137790717 1101 36-37 0.05072593052257446 1101 38-39 -0.12338472068438477 1101 40-41 -0.0773679215763039 1101 42-43 0.17116860548291868 1101 44-45 0.27045614324596556 1101 46-47 0.19539685766271475 1101 48-49 0.3215006945426637 1101 50-51 0.501868177086358 1101 52-53 0.5868769530226956 1101 54-55 0.6590022601761163 1101 56-57 0.6143830164271975 1101 58-59 0.43793572683321713 1101 60-61 0.5730999214793258 1101 62-63 0.6253285061107832 1101 64-65 0.5483134453303009 1101 66-67 0.5261809414627443 1101 68-69 0.2951352818939945 1101 70-71 0.44990218222187295 1101 72-73 0.4647494858783219 1101 74-75 0.4162177036138175 1101 76-77 0.3740326318021907 1101 78-79 0.46262933355008684 1101 80-81 0.39960539173595677 1101 82-83 0.6133239376835569 1101 84-85 0.6040117019122349 1101 86-87 0.5736012957110646 1101 88-89 0.39175737817069844 1101 90-91 0.25806817159046247 1101 92-93 0.389020955775333 1101 94-95 0.301555967266367 1101 96-97 0.5675907196046062 1101 98-99 0.5998070965125741 1101 100-101 0.5896517575609543 1102 1 0.1695158132974619 1102 2 0.038135029497929906 1102 3 0.016905679444192856 1102 4 0.10781054089589048 1102 5 0.17846969522881295 1102 6 0.07820117994960896 1102 7 0.22441400512110476 1102 8 0.13907272389020875 1102 9 0.18448569794726666 1102 10-11 0.18214305399576602 1102 12-13 0.0191510581416523 1102 14-15 0.13757892629369195 1102 16-17 0.0975861486420655 1102 18-19 0.12708132433900232 1102 20-21 0.12827119278639998 1102 22-23 0.08854127306809545 1102 24-25 0.11922082515064858 1102 26-27 0.009295727742404836 1102 28-29 0.03818954579347178 1102 30-31 -0.06314355052828091 1102 32-33 -0.03709250225391969 1102 34-35 0.295567006581976 1102 36-37 0.23892428781667263 1102 38-39 0.4513965138077367 1102 40-41 0.2825924525062149 1102 42-43 0.24344506617814687 1102 44-45 0.3494406703275956 1102 46-47 0.30000643347234757 1102 48-49 0.07641154860370136 1102 50-51 -0.1256420109325589 1102 52-53 0.21646310845479633 1102 54-55 0.13646177107695934 1102 56-57 0.1849201196265966 1102 58-59 0.0027046725882797773 1102 60-61 0.061631052327769 1102 62-63 0.048745418531567 1102 64-65 -0.10589535429645736 1102 66-67 -0.09316336221880661 1102 68-69 -0.030320719353440495 1102 70-71 -0.09705242617827636 1102 72-73 -0.07236732642576271 1102 74-75 -0.05748340905521232 1102 76-77 0.08819141020519439 1102 78-79 0.090768190091147 1102 80-81 0.04996421543279794 1102 82-83 0.033598126261836114 1102 84-85 0.1345479140853847 1102 86-87 0.18636376172521807 1102 88-89 0.14220691329012425 1102 90-91 0.08566634845174903 1102 92-93 0.2666898578453605 1102 94-95 0.36663739097781445 1102 96-97 0.4426199564034974 1102 98-99 -0.26055918952912904 1102 100-101 0.23531227408392041 1103 1 0.03958512478555676 1103 2 -0.13099033823987227 1103 3 -0.22795933326402107 1103 4 0.0013750459424883843 1103 5 -0.09430269758520637 1103 6 -0.07131951009662174 1103 7 0.04777311067603307 1103 8 0.021455331309674364 1103 9 0.082010097058685 1103 10-11 -0.06811944908956491 1103 12-13 -0.03147968307100513 1103 14-15 -0.21970144834858019 1103 16-17 0.03178626471713386 1103 18-19 -0.06600553849896329 1103 20-21 -0.1874062786451276 1103 22-23 -0.14055220738259777 1103 24-25 -0.26773207105556907 1103 26-27 -0.26109045532264474 1103 28-29 -0.16716858579829363 1103 30-31 -0.11095713664730056 1103 32-33 -0.21926564088357026 1103 34-35 -0.23744500785402778 1103 36-37 -0.025826917963058804 1103 38-39 -0.2298520269152604 1103 40-41 -0.1289556819540678 1103 42-43 0.003032932998685567 1103 44-45 -0.30568230919916317 1103 46-47 0.102549411264647 1103 48-49 0.2749519380555441 1103 50-51 0.22298743610986804 1103 52-53 0.23462018336323354 1103 54-55 0.47026629800436837 1103 56-57 0.326285535929145 1103 58-59 0.20271401196107774 1103 60-61 0.3013834255291101 1103 62-63 0.34020389501301906 1103 64-65 0.23910774770950383 1103 66-67 0.32575420447432535 1103 68-69 0.27487664160664593 1103 70-71 0.5563707874999011 1103 72-73 0.402131516159109 1103 74-75 0.3119568289653749 1103 76-77 0.3358809160856673 1103 78-79 0.3610763226835587 1103 80-81 0.416336469490421 1103 82-83 0.22903851104399564 1103 84-85 0.24926076172685896 1103 86-87 0.32322359775251286 1103 88-89 0.3332177669755083 1103 90-91 0.3132088259205119 1103 92-93 0.3679900319579481 1103 94-95 0.15229544985588817 1103 96-97 -0.057805652156705634 1103 98-99 0.18809293884722322 1103 100-101 0.6420806509676389 1104 1 0.05870756666171317 1104 2 0.01700684880062653 1104 3 0.11102359718626076 1104 4 -0.07833968018464788 1104 5 0.1747938167601788 1104 6 0.016787211882110853 1104 7 0.19982919938370003 1104 8 0.09765954080412342 1104 9 0.2075817458365421 1104 10-11 0.20268046851061428 1104 12-13 0.20015829756503223 1104 14-15 0.237284422923004 1104 16-17 0.266355121845784 1104 18-19 0.20118086268873014 1104 20-21 0.08424320955251119 1104 22-23 0.06783197469260216 1104 24-25 0.06300803563799917 1104 26-27 0.21401414272959585 1104 28-29 0.1305838879722785 1104 30-31 0.08269381485756355 1104 32-33 0.14256335106824025 1104 34-35 0.09379091846916765 1104 36-37 0.17110866100225053 1104 38-39 0.12205417935422957 1104 40-41 0.2135093513965458 1104 42-43 0.40373759356113936 1104 44-45 0.37332846670883413 1104 46-47 0.35168527862934695 1104 48-49 0.39769428943396434 1104 50-51 0.15558366986964245 1104 52-53 0.18089194650636387 1104 54-55 0.06779146191608731 1104 56-57 0.17361571060256153 1104 58-59 0.07234470447477293 1104 60-61 0.03235936620123425 1104 62-63 0.4054666865201568 1104 64-65 0.5394053815559445 1104 66-67 0.4916193671074218 1104 68-69 0.49566554888553327 1104 70-71 0.5203002646161927 1104 72-73 0.522824527091295 1104 74-75 0.28123945325769384 1104 76-77 0.5144782721878691 1104 78-79 0.4515456465077605 1104 80-81 0.44230875189450813 1104 82-83 0.5922199966559916 1104 84-85 0.7418007362965682 1104 86-87 0.8109254240451627 1104 88-89 0.8309631373129953 1104 90-91 1.1504855117427688 1104 92-93 1.1599993800758988 1104 94-95 1.1700895497443646 1104 96-97 1.1838758725241725 1104 98-99 0.8783534217386055 1104 100-101 1.0823322545676604 1105 1 0.22044318289402653 1105 2 0.14222856869425726 1105 3 0.06307959689110731 1105 4 0.23112311202443436 1105 5 0.28915303562502714 1105 6 0.1500759883856233 1105 7 0.08238080158486838 1105 8 0.15537198748492642 1105 9 0.28013842808390876 1105 10-11 0.2018198405714955 1105 12-13 0.20074505709154522 1105 14-15 0.16099504192238356 1105 16-17 0.18349077043145456 1105 18-19 0.1902921100245365 1105 20-21 0.10570995227797653 1105 22-23 0.32762312519535186 1105 24-25 0.22965696471317898 1105 26-27 0.2090866398135347 1105 28-29 0.14582118856628057 1105 30-31 0.25967808374242196 1105 32-33 0.2802020945088728 1105 34-35 0.19124692214722927 1105 36-37 0.158762587821208 1105 38-39 0.25008908125824547 1105 40-41 0.27320415130233044 1105 42-43 0.17090261559831532 1105 44-45 0.13855370574061254 1105 46-47 0.2593135781761191 1105 48-49 -0.05126401729297925 1105 50-51 -0.04989994201482517 1105 52-53 0.13417254952771884 1105 54-55 0.22610372299072878 1105 56-57 0.3608771399970152 1105 58-59 0.2720203194015909 1105 60-61 0.5825198559290428 1105 62-63 0.45674606351918356 1105 64-65 0.47678033153594157 1105 66-67 0.5016638104166624 1105 68-69 0.3816617913541549 1105 70-71 0.5564246434457374 1105 72-73 0.8668146053921149 1105 74-75 0.8050702621770078 1105 76-77 0.6988860778633317 1105 78-79 0.716340216818157 1105 80-81 0.844810177996866 1105 82-83 1.0069610703904495 1105 84-85 0.9840139336322409 1105 86-87 0.914805983361088 1105 88-89 1.110294018601678 1105 90-91 1.1287179394656306 1105 92-93 1.4576035281180957 1105 94-95 1.5435658906353318 1105 96-97 1.643009880737388 1105 98-99 1.4013526923688424 1105 100-101 1.5242487374728775 1106 1 -0.2304028340528781 1106 2 0.05215499045429084 1106 3 -0.06145887895025837 1106 4 0.003694631667435999 1106 5 0.11573948341635543 1106 6 -0.10530956690431026 1106 7 -0.3969668223957967 1106 8 -0.3017826330691733 1106 9 -0.43125955847544617 1106 10-11 -0.6657725306665867 1106 12-13 -0.3845804668545938 1106 14-15 -0.3620811660091974 1106 16-17 -0.09936045277358119 1106 18-19 -0.4910038763754372 1106 20-21 -0.373372046287642 1106 22-23 -0.2839461897566977 1106 24-25 -0.17760656226108296 1106 26-27 -0.2908934382803352 1106 28-29 -0.035122844158777866 1106 30-31 -0.1578053810754909 1106 32-33 0.017080199005917507 1106 34-35 0.24366809780532606 1106 36-37 0.032650281395731895 1106 38-39 0.1261681845068523 1106 40-41 -0.018465835682029308 1106 42-43 0.17059714637102275 1106 44-45 -0.12059044917212702 1106 46-47 0.0677312475787204 1106 48-49 0.2538067718801287 1106 50-51 0.12555892588965634 1106 52-53 0.24967976125773816 1106 54-55 -0.046952563034714956 1106 56-57 -0.011589457607044551 1106 58-59 -0.07114115428821322 1106 60-61 -0.052577230160153476 1106 62-63 0.02164499304876699 1106 64-65 0.20462053128624547 1106 66-67 0.2645067992338319 1106 68-69 -0.19699653467448286 1106 70-71 0.11192043328291135 1106 72-73 0.03650940179023365 1106 74-75 0.25246314872839193 1106 76-77 0.4900580171944888 1106 78-79 0.6402983011276611 1106 80-81 0.5155807573258961 1106 82-83 0.37461984798229153 1106 84-85 0.158184518273778 1106 86-87 0.031010461805585976 1106 88-89 0.619252235723895 1106 90-91 0.6365406079385494 1106 92-93 0.5330677085308757 1106 94-95 0.35560270631785684 1106 96-97 0.7260178229372976 1106 98-99 0.9085909849286757 1106 100-101 1.400102059118698 1107 1 -0.008953160995684328 1107 2 -0.0756663590963953 1107 3 0.08998547896867848 1107 4 -0.001810553682560112 1107 5 0.003554528965850068 1107 6 0.015163294988617793 1107 7 0.08578060965078294 1107 8 0.040193980322506206 1107 9 0.10080586716824058 1107 10-11 0.02081663386784527 1107 12-13 0.0030514845428015747 1107 14-15 0.07168278622872748 1107 16-17 0.05820403963071641 1107 18-19 0.16836451832919863 1107 20-21 0.06739042883627633 1107 22-23 0.17959103475227067 1107 24-25 0.22203080316084822 1107 26-27 0.14920232691416402 1107 28-29 0.1859181503901226 1107 30-31 0.12465713483902618 1107 32-33 0.004324639742129932 1107 34-35 -0.04055737530379133 1107 36-37 -0.02585254922441038 1107 38-39 0.11653835916477107 1107 40-41 0.15086226285816196 1107 42-43 0.3735071845658595 1107 44-45 0.3032586070818084 1107 46-47 0.43728709361276685 1107 48-49 0.5450160894410985 1107 50-51 0.4091516812326077 1107 52-53 0.11235760207551948 1107 54-55 0.0974654602282854 1107 56-57 0.10130237932945363 1107 58-59 0.1025580274277118 1107 60-61 0.39651641515943226 1107 62-63 0.553071520532967 1107 64-65 0.41716812031481965 1107 66-67 0.32659271288138214 1107 68-69 0.4109054072050995 1107 70-71 0.43918539292494074 1107 72-73 0.44296065084772707 1107 74-75 0.3770104349176826 1107 76-77 0.19257946315930852 1107 78-79 0.26643472094941956 1107 80-81 0.3227918857421095 1107 82-83 0.6145187676495389 1107 84-85 0.38138478773892714 1107 86-87 0.5878129117134954 1107 88-89 0.12400882093297838 1107 90-91 0.298275320735673 1107 92-93 0.4557602586847658 1107 94-95 0.5816923096602125 1107 96-97 0.6421599287209112 1107 98-99 0.834804814176767 1107 100-101 0.926578864102563 1108 1 0.0869151956329155 1108 2 0.10599299136971041 1108 3 0.034824542655805146 1108 4 -8.231680986909851E-4 1108 5 0.07295039973441675 1108 6 0.06849266872989546 1108 7 0.18580653794397506 1108 8 0.07743123023507081 1108 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78-79 -0.5081069125768138 2316 80-81 -0.21955987367205765 2316 82-83 -0.27590010457009484 2316 84-85 -0.34484666504624073 2316 86-87 -0.5180227757333604 2316 88-89 -0.61068886975022 2316 90-91 -0.29378194593282103 2316 92-93 -0.12924152404198352 2316 94-95 -0.21241303577737725 2316 96-97 -0.07645208137206794 2316 98-99 -0.2875073824168517 2316 100-101 -0.45923063097436767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2933.0 3 560.0 4 408.0 5 406.0 6 481.0 7 608.0 8 858.0 9 1105.0 10 1412.0 11 2257.0 12 3552.0 13 5341.0 14 5925.0 15 8542.0 16 11933.0 17 17482.0 18 13276.0 19 6543.0 20 5755.0 21 5733.0 22 5950.0 23 6421.0 24 6967.0 25 7890.0 26 8936.0 27 10184.0 28 12115.0 29 14578.0 30 17663.0 31 21719.0 32 27398.0 33 35708.0 34 49493.0 35 71500.0 36 101106.0 37 144901.0 38 128032.0 39 17501.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.91228784375776 19.594544425753956 15.632032936224471 18.861134794263812 2 14.945347183917828 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21.444089710387264 25.31495712794679 34.78398871676928 18.456964444896663 22-23 20.444769238842053 25.940200857747307 34.67336619103627 18.941663712374368 24-25 19.53107603473738 26.104483456743356 35.304502396961375 19.059938111557884 26-27 19.576719576719576 26.17198198959205 35.210462726185995 19.040835707502374 28-29 19.16575466082983 26.413417045869092 35.33270612931923 19.08812216398185 30-31 19.35071991828318 26.249736794778006 35.24921134435307 19.15033194258574 32-33 19.04637863888151 26.472976994684682 34.953998969576915 19.52664539685689 34-35 19.424915383551895 26.39322709979364 34.987045106583444 19.194812410071023 36-37 19.535278981711794 26.313031091680365 34.72161341165073 19.430076514957115 38-39 19.43030247183764 26.610329143214027 34.66613426299206 19.293234121956278 40-41 19.465105683732027 26.744715813398724 34.403458973007005 19.386719529862244 42-43 19.273329033050743 26.959828193471765 33.88622680919032 19.880615964287177 44-45 19.581169257157853 27.049656147896407 33.39778360198279 19.971390992962952 46-47 19.858164199261378 27.515825316666348 32.47073356503261 20.155276919039665 48-49 19.937759110347002 27.839356545438605 31.716866679425493 20.5060176647889 50-51 20.041668800245787 28.125160018434126 31.41936351067643 20.413807670643656 52-53 20.13419209444665 28.616784255477164 30.752909842887693 20.496113807188497 54-55 19.862306985270585 28.461655644568708 30.828270337114898 20.847767033045812 56-57 20.306433974446346 28.66771481829159 30.2329673761207 20.792883831141364 58-59 20.377463509195078 28.147325926944816 30.660657106390328 20.814553457469778 60-61 20.39412529020726 28.208881413748387 31.09716737241916 20.29982592362519 62-63 20.496102009936116 28.248511661980118 30.990956407131225 20.264429920952537 64-65 20.26984665186711 28.473491948743213 30.999033977135326 20.25762742225435 66-67 20.379133881691956 28.483470870820536 30.60164840261815 20.535746844869358 68-69 20.519430001260382 28.457902996824743 30.5965178970724 20.42614910484248 70-71 20.37899440254224 28.65918846139328 30.4246045024059 20.537212633658577 72-73 20.270661932983273 28.618289514345324 30.35663215786736 20.754416394804036 74-75 20.03387232169364 29.092063102075077 30.1639614876693 20.710103088561983 76-77 19.82864043095223 28.864725257074547 30.407716491998677 20.898917819974542 78-79 19.7916280069773 28.75058709404127 30.391442952375957 21.066341946605476 80-81 19.697803428264304 29.032177568478907 30.14012502479381 21.129893978462977 82-83 19.807241125817264 28.932833180527822 29.999379394224786 21.260546299430132 84-85 19.8169775902156 28.543076102158828 29.944823279662288 21.695123027963287 86-87 19.705685498887267 28.568137812592383 29.967852942569127 21.758323745951223 88-89 19.765978371523254 28.220868709477 30.00184918933203 22.011303729667716 90-91 19.633343849569613 28.208513287893805 30.234605925909808 21.92353693662677 92-93 19.5818755582628 28.183601598071256 30.397521505114906 21.837001338551037 94-95 19.324135142259724 27.96605831663378 30.562551026414724 22.147255514691768 96-97 19.284110919999183 28.044758584679645 30.403241906751028 22.26788858857014 98-99 19.29168349157816 27.956763550426423 30.22007944131268 22.53147351668274 100-101 19.429669616798478 27.695839149788814 30.841779189223423 22.03271204418928 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1801.0 1 1182.5 2 712.5 3 843.5 4 696.5 5 650.5 6 1073.0 7 2384.5 8 4039.5 9 4582.0 10 3806.0 11 3018.0 12 2952.0 13 3047.0 14 3078.5 15 3096.0 16 3088.5 17 3190.0 18 3365.0 19 3421.5 20 3538.5 21 3965.0 22 4475.0 23 4999.0 24 5725.5 25 6665.5 26 7714.0 27 8979.0 28 10081.5 29 11176.0 30 12813.0 31 14284.5 32 15925.5 33 17629.5 34 19333.0 35 20773.0 36 22667.0 37 24747.0 38 25280.5 39 25184.0 40 24857.5 41 24676.5 42 24490.5 43 24853.0 44 25830.0 45 26337.0 46 26313.5 47 26751.5 48 29560.0 49 28971.0 50 24906.5 51 21644.5 52 18837.0 53 17603.0 54 16553.5 55 15211.5 56 13976.5 57 12773.5 58 11813.5 59 10910.0 60 9647.5 61 8463.5 62 6998.0 63 4763.0 64 2972.5 65 2188.0 66 1575.0 67 1106.5 68 814.0 69 576.0 70 440.0 71 326.0 72 261.0 73 281.0 74 229.0 75 165.5 76 128.5 77 84.5 78 56.0 79 41.5 80 33.0 81 20.5 82 13.0 83 11.0 84 7.5 85 4.0 86 2.0 87 2.5 88 2.0 89 1.0 90 2.5 91 1.5 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2589462877981138 2 0.2281740711514938 3 0.2170654286276102 4 0.2239604481251931 5 0.2256203602264631 6 0.2540942370405555 7 0.25473266477181317 8 0.24349633670167803 9 0.238005858212862 10-11 0.24847607300548794 12-13 0.24949755737550022 14-15 0.23404760627906443 16-17 0.2512851550230217 18-19 0.2399849841797608 20-21 0.23462219123719635 22-23 0.23302612190905214 24-25 0.22574804577271462 26-27 0.23596288947283747 28-29 0.24630541871921183 30-31 0.24764611695485295 32-33 0.247901488047356 34-35 0.2556264635955739 36-37 0.23283459358967484 38-39 0.23136620980778216 40-41 0.22804638560524224 42-43 0.23366454964030983 44-45 0.24917834350987136 46-47 0.25396655149430397 48-49 0.25735021846996964 50-51 0.25715869015059234 52-53 0.28141894393838407 54-55 0.2574140612430954 56-57 0.22964245493338645 58-59 0.2095958241718954 60-61 0.20691442770061313 62-63 0.21470324602195678 64-65 0.20672289938123584 66-67 0.20806359761687696 68-69 0.21240490618942917 70-71 0.21176647845817148 72-73 0.21483093156820834 74-75 0.21834228409012557 76-77 0.23423913459844176 78-79 0.22766332896648764 80-81 0.22076830946890472 82-83 0.21444787492945375 84-85 0.21515014543383718 86-87 0.22536498913396003 88-89 0.22351354871331272 90-91 0.235260618968454 92-93 0.22115136610765937 94-95 0.21910839736763477 96-97 0.22753564342023613 98-99 0.235260618968454 100-101 0.20774438375124812 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 783174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.48025140571965 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42523440293631 44.29476955630337 2 12.397501510136923 14.004280041906448 3 3.930180127203278 6.659326849626132 4 1.7543325984204785 3.9634058483215218 5 0.9177628897282855 2.591773937134666 6 0.5444559322554023 1.8450604759872355 7 0.36864045667425743 1.457463396989695 8 0.2726455808458704 1.2319272760666578 9 0.2088719404683712 1.06174257383287 >10 1.0647519079851564 10.936257426753897 >50 0.07195347535652143 2.788653162231285 >100 0.038691344231160184 3.8640137733217506 >500 0.003167712391459805 1.1763542390481105 >1k 0.0011313258540927875 0.9373773322561533 >5k 4.5253034163711506E-4 1.7734131206160382 >10k+ 2.2626517081855753E-4 1.4141809896041708 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11066 1.4129682548194908 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8107 1.0351467234611977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5770 0.7367456018713594 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1864 0.238005858212862 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1864 0.238005858212862 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1333 0.1702048331532967 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1178 0.15041357348430873 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1096 0.1399433586916828 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.10674511666628361 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.10176538036247373 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 3.830566387546063E-4 0.0 26-27 0.0 0.0 0.0 0.001085327143138051 0.0 28-29 0.0 0.0 0.0 0.002489868151904941 0.0 30-31 0.0 0.0 0.0 0.004852050757558346 0.0 32-33 0.0 0.0 0.0 0.007916503867595195 0.0 34-35 0.0 0.0 0.0 0.01002331538074553 0.0 36-37 0.0 0.0 0.0 0.013215454037033916 0.0 38-39 0.0 0.0 0.0 0.016854492105202675 0.0 40-41 0.0 0.0 0.0 0.02540942370405555 0.0 42-43 0.0 0.0 0.0 0.03511352521917224 0.0 44-45 0.0 0.0 0.0 0.05796923799819708 0.0 46-47 0.0 0.0 0.0 0.10744738717066706 0.0 48-49 0.0 0.0 0.0 0.14511462331487002 0.0 50-51 0.0 0.0 0.0 0.19657189845423878 0.0 52-53 0.0 0.0 0.0 0.2528812243511659 0.0 54-55 0.0 0.0 0.0 0.3137233871400225 0.0 56-57 0.0 0.0 0.0 0.38624877741089464 0.0 58-59 0.0 0.0 0.0 0.5000804418941385 0.0 60-61 0.0 0.0 0.0 0.646152706805895 0.0 62-63 0.0 0.0 0.0 0.815719112227934 0.0 64-65 0.0 0.0 0.0 0.9526618605827057 0.0 66-67 0.0 0.0 0.0 1.1407426702112176 0.0 68-69 0.0 0.0 0.0 1.3813022393491101 0.0 70-71 0.0 0.0 0.0 1.5824708174684043 0.0 72-73 0.0 0.0 0.0 1.9046852934341538 0.0 74-75 0.0 0.0 0.0 2.1626100968622555 0.0 76-77 0.0 0.0 0.0 2.4002328984363626 0.0 78-79 0.0 0.0 0.0 2.7103172475082165 0.0 80-81 0.0 0.0 0.0 3.0123574071662236 0.0 82-83 0.0 0.0 0.0 3.3926049639032962 0.0 84-85 0.0 0.0 0.0 3.842760357213084 0.0 86-87 0.0 0.0 0.0 4.394553445339094 0.0 88-89 0.0 0.0 0.0 5.071159154925981 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5915 0.0 36.864677 1 GTATCAA 9665 0.0 36.029198 1 ATCAACG 12225 0.0 28.325365 3 TATCAAC 12300 0.0 28.152649 2 TCAACGC 12375 0.0 28.024002 4 CAACGCA 12470 0.0 27.770632 5 AACGCAG 12750 0.0 27.272537 6 CCGTCGT 70 0.008389646 27.143059 9 ACAGCGT 90 8.9445745E-4 26.389086 8 GTACATG 8665 0.0 25.548761 1 TACATGG 8695 0.0 25.07494 2 ACGCAGA 14370 0.0 24.0988 7 CGCAGAG 14420 0.0 24.046642 8 GAGTACT 7480 0.0 23.939154 12-13 ACATGGG 8815 0.0 23.817533 3 ACCGCTC 100 0.0016531001 23.750175 8 AGTACTT 7555 0.0 23.732937 12-13 GCAGAGT 14710 0.0 23.088207 9 TATATGG 290 3.6379788E-12 22.931204 2 CATGGGG 1735 0.0 22.726425 4 >>END_MODULE