FastQCFastQC Report
Thu 26 May 2016
SRR937460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783174
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107791.3763225030453003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91371.166662836100279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62980.80416357029217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24350.31091430512248874No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.26341528191691754No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.21361791887881876No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13860.1769721671046281No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.1399433586916828No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.12564257751151084No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.10572363229627132No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.1038083491024983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.033.58941
TACGGTG600.00395020431.6666455
CTTACGG600.00395020431.6666453
TTTCGCG300.00395263531.66462382-83
GTATCAA107400.030.6690041
TCTTACG650.005842969329.2307512
TAACGCA700.00838999327.142844
ACGGTGT700.00839260627.1411086
GTACACG908.911728E-426.405731
ACCGTGC950.001225297725.0031768
GTACATG90000.024.979821
TACATGG91000.024.5329512
ATCAACG135800.024.2396743
TATCAAC136000.024.204032
TCAACGC137350.024.0352944
CAACGCA138600.023.8527975
GAGTACT78850.023.64758312-13
ACATGGG90350.023.500263
AACGCAG142100.023.4643556
CATGGGG17550.023.0056824
Produced by FastQC (version 0.11.5)