##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937460_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 783174 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28724140484746 33.0 31.0 34.0 31.0 34.0 2 32.460822243843644 34.0 31.0 34.0 31.0 34.0 3 32.474304560672344 34.0 31.0 34.0 31.0 34.0 4 36.134826998853384 37.0 35.0 37.0 35.0 37.0 5 36.08754887164283 37.0 35.0 37.0 35.0 37.0 6 36.15977420088001 37.0 36.0 37.0 35.0 37.0 7 36.10650889840572 37.0 35.0 37.0 35.0 37.0 8 36.09584715529372 37.0 35.0 37.0 35.0 37.0 9 37.78798964214849 39.0 38.0 39.0 35.0 39.0 10-11 37.72294917859888 39.0 37.0 39.0 35.0 39.0 12-13 37.62761148863471 39.0 37.0 39.0 35.0 39.0 14-15 38.72924471445681 40.0 38.0 41.0 34.5 41.0 16-17 38.730850998628654 40.0 38.0 41.0 34.5 41.0 18-19 38.84414702224537 40.0 38.0 41.0 35.0 41.0 20-21 38.82609037072221 40.0 38.0 41.0 35.0 41.0 22-23 38.68073952914678 40.0 38.0 41.0 34.5 41.0 24-25 38.60095010814966 40.0 38.0 41.0 34.0 41.0 26-27 38.50074887572877 40.0 38.0 41.0 34.0 41.0 28-29 38.350601526608386 40.0 38.0 41.0 34.0 41.0 30-31 38.246366069353684 40.0 37.5 41.0 34.0 41.0 32-33 38.02895078232934 40.0 36.5 41.0 34.0 41.0 34-35 37.837011953920836 40.0 36.0 41.0 33.5 41.0 36-37 37.61463736028009 40.0 36.0 41.0 33.0 41.0 38-39 37.44413757351495 40.0 36.0 41.0 33.0 41.0 40-41 37.18257883944053 40.0 35.0 41.0 33.0 41.0 42-43 36.82752747154528 40.0 35.0 41.0 32.5 41.0 44-45 36.314412250662045 40.0 35.0 41.0 30.5 41.0 46-47 35.620706254293424 39.0 35.0 41.0 28.0 41.0 48-49 35.03891217022016 39.0 35.0 41.0 24.0 41.0 50-51 34.70688059103085 39.0 35.0 41.0 22.5 41.0 52-53 34.44350872219967 39.0 35.0 41.0 20.5 41.0 54-55 34.21107123065883 38.5 34.5 40.0 18.5 41.0 56-57 33.953947654033456 38.0 34.0 40.0 16.0 41.0 58-59 33.69721543360735 38.0 34.0 40.0 13.0 41.0 60-61 33.379438030373834 38.0 34.0 40.0 8.0 41.0 62-63 33.0323874388067 37.0 33.0 40.0 6.5 41.0 64-65 32.65038675951959 36.5 33.0 40.0 2.0 41.0 66-67 32.245641453878704 36.0 32.5 39.0 2.0 41.0 68-69 31.831877079678335 35.5 32.0 39.0 2.0 40.0 70-71 31.43235922030098 35.0 31.5 38.5 2.0 40.0 72-73 30.9834928125806 35.0 31.0 38.0 2.0 40.0 74-75 30.524658632692095 35.0 31.0 37.0 2.0 39.0 76-77 29.272775909312617 33.5 29.5 35.5 2.0 38.5 78-79 29.79514322487723 34.0 30.0 36.0 2.0 39.0 80-81 29.579721747657608 34.0 30.0 36.0 2.0 37.5 82-83 29.2313604639582 34.0 30.0 35.0 2.0 37.0 84-85 28.8637224933412 34.0 29.5 35.0 2.0 37.0 86-87 28.459286825149967 34.0 29.0 35.0 2.0 36.0 88-89 28.00324385130252 34.0 29.0 35.0 2.0 36.0 90-91 27.516877475503527 34.0 27.0 35.0 2.0 35.5 92-93 27.214581944753018 34.0 27.0 35.0 2.0 35.0 94-95 26.83313222859799 33.5 26.0 35.0 2.0 35.0 96-97 26.435965698554856 33.0 25.0 35.0 2.0 35.0 98-99 25.79895655371603 33.0 23.5 35.0 2.0 35.0 100-101 23.63405833186495 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.036490811083140784 1101 2 -0.060243980758997395 1101 3 -0.01975455624996414 1101 4 -0.01157109996600525 1101 5 -0.08020061504753073 1101 6 0.016986310881740962 1101 7 0.011453343922646297 1101 8 0.0387621394529063 1101 9 -0.08986411967577368 1101 10-11 -0.04994151926997148 1101 12-13 -0.040718474322581244 1101 14-15 -0.2372300211040823 1101 16-17 -0.12031051586361485 1101 18-19 -0.1165883543543913 1101 20-21 -0.09784230195193544 1101 22-23 -0.04991803161509978 1101 24-25 0.1027957039830838 1101 26-27 -0.09473514270756311 1101 28-29 -0.23894932318027884 1101 30-31 -0.15874839526856732 1101 32-33 -0.060264727176821964 1101 34-35 -0.018636823541200442 1101 36-37 -0.0018175308287098346 1101 38-39 -1.716058830609768E-4 1101 40-41 -0.014923644975667116 1101 42-43 -0.21380730254411873 1101 44-45 -0.1561060608772209 1101 46-47 -0.022807407762606147 1101 48-49 0.16821508582216183 1101 50-51 -0.0017415262462847636 1101 52-53 -0.10923663664515004 1101 54-55 -0.11669685722537082 1101 56-57 -0.4205201625870032 1101 58-59 -0.2907236504061146 1101 60-61 -0.13622684938477647 1101 62-63 -0.26267953887833784 1101 64-65 -0.21310517404098306 1101 66-67 -0.03789977597435268 1101 68-69 -0.12243153597290402 1101 70-71 -0.18045905117362082 1101 72-73 -0.1285110855280429 1101 74-75 -0.007330464662850034 1101 76-77 0.03150975283461577 1101 78-79 -0.382202882592928 1101 80-81 -0.37823421119093936 1101 82-83 0.07272246109712555 1101 84-85 -0.07757487791824502 1101 86-87 -0.09571317240997246 1101 88-89 -0.15463857694281558 1101 90-91 -0.241159794470569 1101 92-93 -0.18721410802774585 1101 94-95 -0.17956587850781247 1101 96-97 -0.2812661388996922 1101 98-99 -0.2547337775627163 1101 100-101 0.055858321464992144 1102 1 -0.04971512570450898 1102 2 -0.12019148446232464 1102 3 -0.029441991666033118 1102 4 0.020593710189714898 1102 5 0.012810150282142274 1102 6 0.014351595756444624 1102 7 -0.06375174680734119 1102 8 -0.04715259180986209 1102 9 0.0028409741480572848 1102 10-11 -0.061521147934776366 1102 12-13 -0.2307700424669008 1102 14-15 -0.05930816658144522 1102 16-17 -0.05791392750413138 1102 18-19 -0.13927672906062583 1102 20-21 -0.06101631071617675 1102 22-23 -0.12399510786095647 1102 24-25 -0.1582038294656698 1102 26-27 -0.0943954389490429 1102 28-29 -0.2147499164552613 1102 30-31 -0.055498253261262676 1102 32-33 -0.07091496595948144 1102 34-35 -0.22176482069981063 1102 36-37 -0.14752816757835063 1102 38-39 -0.22022105784004253 1102 40-41 -0.1500409696198517 1102 42-43 -0.09113897592854414 1102 44-45 0.25950880292570844 1102 46-47 0.1520472126416479 1102 48-49 0.31566012992177406 1102 50-51 0.3064465099937337 1102 52-53 0.3422828724983873 1102 54-55 0.47326338072813456 1102 56-57 0.3782137138598287 1102 58-59 0.2557811134904924 1102 60-61 0.3159287534996764 1102 62-63 0.38847625829934174 1102 64-65 0.25667851696100996 1102 66-67 0.20988410560825344 1102 68-69 0.18660297520638736 1102 70-71 0.4362220740440286 1102 72-73 0.36369985998694077 1102 74-75 0.3398220258174014 1102 76-77 0.392377241142988 1102 78-79 0.2729780019238852 1102 80-81 0.22798787462726366 1102 82-83 0.231015749813146 1102 84-85 0.2959173385961833 1102 86-87 0.40554725847547246 1102 88-89 0.3485499357487356 1102 90-91 0.20666566833712707 1102 92-93 0.3235017201873447 1102 94-95 0.4063921665310879 1102 96-97 0.33735668102513827 1102 98-99 0.5112344647766243 1102 100-101 1.0665945690316825 1103 1 0.16379627833676835 1103 2 0.14451702704177904 1103 3 0.05523943565697209 1103 4 0.007018264734988122 1103 5 0.05752306463342194 1103 6 -0.011625202573142701 1103 7 0.057673441481085774 1103 8 -0.11575562176923171 1103 9 0.025379549093187848 1103 10-11 0.06248971242158774 1103 12-13 -0.11226674366701417 1103 14-15 -0.02747157892142127 1103 16-17 -0.12362308609334605 1103 18-19 0.0029765947554381 1103 20-21 -0.0801466816893992 1103 22-23 0.035209177576959405 1103 24-25 0.08383935846251944 1103 26-27 -0.015204228633038497 1103 28-29 -0.18521748548323558 1103 30-31 -0.12933666876995886 1103 32-33 0.08413930246743462 1103 34-35 0.17221528643613482 1103 36-37 -0.1394502772092423 1103 38-39 0.035768482062429996 1103 40-41 0.0703308708369832 1103 42-43 0.029942656860661998 1103 44-45 -0.22218469220113946 1103 46-47 -0.2045954162583712 1103 48-49 -0.05704488598851043 1103 50-51 -0.1515963577273851 1103 52-53 -0.03409585241038826 1103 54-55 -0.13127021766549518 1103 56-57 -0.04633482753026641 1103 58-59 -0.05958549024659732 1103 60-61 0.03187439835001271 1103 62-63 0.06996161883248675 1103 64-65 -0.057578702707708374 1103 66-67 0.14142783722136443 1103 68-69 0.034890868043749634 1103 70-71 0.05606690703602979 1103 72-73 0.15788009653991253 1103 74-75 0.2885768872944894 1103 76-77 0.31005533964668786 1103 78-79 0.2027379133332623 1103 80-81 0.3551707862434412 1103 82-83 0.2255235315119748 1103 84-85 0.10560960626261107 1103 86-87 0.0805584323679227 1103 88-89 0.2561928781848479 1103 90-91 0.4034949512734265 1103 92-93 0.5244883582132687 1103 94-95 0.5487455550556675 1103 96-97 0.604947723232435 1103 98-99 0.7025301693322348 1103 100-101 0.8881818621478992 1104 1 -0.001223575758714901 1104 2 -0.013501683400974684 1104 3 -0.019666266268771437 1104 4 -0.03021082808557196 1104 5 -0.013421763001773002 1104 6 0.12761629284159426 1104 7 0.0495115968615778 1104 8 0.07757283387337566 1104 9 0.026577187912025124 1104 10-11 0.02673174059380301 1104 12-13 -0.03651218148711166 1104 14-15 -0.02936180246646103 1104 16-17 -0.1006541351993846 1104 18-19 -0.011882366580579173 1104 20-21 -0.023370361693942243 1104 22-23 -0.14015895784289256 1104 24-25 0.05355031977011748 1104 26-27 -0.3323050334520019 1104 28-29 -0.2030719645529615 1104 30-31 -0.267818807187453 1104 32-33 -0.2759933896626734 1104 34-35 -0.38336339474047776 1104 36-37 -0.29494158615885624 1104 38-39 -0.3050606306477448 1104 40-41 -0.3194067993790384 1104 42-43 -0.28839059359928854 1104 44-45 -0.3599010805316212 1104 46-47 -0.3893294282063522 1104 48-49 -0.20387711000237374 1104 50-51 -0.328507358814214 1104 52-53 -0.09423919969012928 1104 54-55 -0.0637762378250315 1104 56-57 -0.007945033742927876 1104 58-59 -0.22147202000642352 1104 60-61 -0.19465834492481093 1104 62-63 -0.013736712571081 1104 64-65 0.051923559735733704 1104 66-67 -0.040692111349784454 1104 68-69 -0.05611192360545303 1104 70-71 0.04836749591063594 1104 72-73 0.265902003527561 1104 74-75 0.3258074008016685 1104 76-77 0.3990556735080446 1104 78-79 0.24036337772019678 1104 80-81 0.3343548859188168 1104 82-83 0.2141630109723991 1104 84-85 0.16664443439504595 1104 86-87 -0.03838003059122386 1104 88-89 0.10024339780097691 1104 90-91 -0.14380823035425294 1104 92-93 0.1460884775509399 1104 94-95 0.14641918071895077 1104 96-97 0.023607731614486482 1104 98-99 0.09351946445857351 1104 100-101 0.8581691470026698 1105 1 0.023770819162372447 1105 2 0.08232529987460424 1105 3 0.051042868448945455 1105 4 0.08365457623218475 1105 5 -0.05022939385553826 1105 6 -0.08156099509486125 1105 7 0.040908155485027464 1105 8 0.0769115764626136 1105 9 -0.016976403737395174 1105 10-11 0.017206653963405927 1105 12-13 0.03838302482235889 1105 14-15 0.04020715987024204 1105 16-17 0.08898200294381553 1105 18-19 0.0763983357712874 1105 20-21 0.005226183307037502 1105 22-23 -0.0454565378678069 1105 24-25 -0.016315215096305735 1105 26-27 0.09232581179389143 1105 28-29 0.013105286489292212 1105 30-31 0.04657503545598729 1105 32-33 0.09162457951081393 1105 34-35 0.19164958917649244 1105 36-37 0.17137756561532314 1105 38-39 0.03925652884016273 1105 40-41 0.07408889677176234 1105 42-43 0.08088548562673026 1105 44-45 0.21853809655765133 1105 46-47 0.004662211002525396 1105 48-49 0.33591327423642525 1105 50-51 0.41148154454855046 1105 52-53 0.3193418355608486 1105 54-55 0.24676145242837322 1105 56-57 0.19716608872024466 1105 58-59 0.3071748997543864 1105 60-61 0.13763698455449713 1105 62-63 0.19444159421261986 1105 64-65 0.32577281575019157 1105 66-67 0.3211171513623938 1105 68-69 0.36470817748463347 1105 70-71 0.21317029646347763 1105 72-73 0.4264988663861793 1105 74-75 0.5215346278627067 1105 76-77 0.5208377196598377 1105 78-79 0.21028893659623193 1105 80-81 0.360424888387886 1105 82-83 0.11785640322291613 1105 84-85 0.31528828910004947 1105 86-87 0.25885588505162715 1105 88-89 0.296520490198219 1105 90-91 0.4306229605618306 1105 92-93 0.5056947313999984 1105 94-95 0.9033198133124145 1105 96-97 0.9693839144280396 1105 98-99 0.8323376867829637 1105 100-101 1.1994013845785538 1106 1 -0.05179961141642053 1106 2 0.12457302213666566 1106 3 0.10169763509701824 1106 4 0.07604440809129898 1106 5 0.04875591705901172 1106 6 0.05740094078316815 1106 7 -0.004659884465034736 1106 8 -0.055635505642200656 1106 9 0.13356680023635903 1106 10-11 -0.06595402285014984 1106 12-13 0.00909782281422622 1106 14-15 -0.003468298997660213 1106 16-17 0.08547574746529563 1106 18-19 0.19205164147957987 1106 20-21 0.1671447956798673 1106 22-23 0.09725735699765181 1106 24-25 -0.008977313005836152 1106 26-27 -0.014827480633130108 1106 28-29 -0.03586275877327694 1106 30-31 0.04652191037298081 1106 32-33 0.05276358129049896 1106 34-35 0.12861667818756928 1106 36-37 0.04812073240152159 1106 38-39 3.88889984478169E-4 1106 40-41 0.10230495648436033 1106 42-43 0.1512961425183832 1106 44-45 0.0912171944937441 1106 46-47 0.0489552489603966 1106 48-49 -0.2686236078524544 1106 50-51 -0.25211050793903667 1106 52-53 -0.05390171638219954 1106 54-55 -0.11485434697131325 1106 56-57 -0.2180927632467089 1106 58-59 -0.056824887745285935 1106 60-61 -0.16526231685066506 1106 62-63 -0.18364962155489906 1106 64-65 -0.22471593501347797 1106 66-67 -0.1685035355449287 1106 68-69 -0.19559543236476173 1106 70-71 -0.14557674845377733 1106 72-73 -0.1986306260036521 1106 74-75 -0.06987371808783394 1106 76-77 -0.029099858367626297 1106 78-79 -0.077635732036061 1106 80-81 -0.16677515411218025 1106 82-83 -0.14863229441567327 1106 84-85 -0.21221722262731646 1106 86-87 -0.2208702551454671 1106 88-89 0.2497571358804791 1106 90-91 0.1459256407222682 1106 92-93 0.19062314535064573 1106 94-95 0.023561798065511397 1106 96-97 0.19449481303881555 1106 98-99 0.16388468920015242 1106 100-101 0.6830189371059774 1107 1 -0.03660356934030773 1107 2 -0.10210989896442868 1107 3 -0.040848501539116455 1107 4 -0.13514735715264692 1107 5 -0.10600731207289016 1107 6 -0.12532547792611837 1107 7 -0.18145158048857013 1107 8 -0.09826631366682648 1107 9 -0.0688951594427536 1107 10-11 -0.08732427785317753 1107 12-13 -0.2386764630413154 1107 14-15 -0.052817967110541986 1107 16-17 -0.12296033490699187 1107 18-19 -0.19163644643033706 1107 20-21 0.030198095391860136 1107 22-23 -0.0539019302793875 1107 24-25 -0.05472690200967634 1107 26-27 -0.24899794824168708 1107 28-29 -0.18199893137929735 1107 30-31 -0.11310915104777308 1107 32-33 -0.19965295249770776 1107 34-35 -0.17844380314146235 1107 36-37 -0.24221405950463293 1107 38-39 -0.29675978555723503 1107 40-41 -0.22810196061184485 1107 42-43 -0.39160737536149526 1107 44-45 -0.5829578775078375 1107 46-47 -0.5903407733384611 1107 48-49 -0.40157004856394707 1107 50-51 -0.33781479465984177 1107 52-53 -0.5332375713160076 1107 54-55 -0.596799100435426 1107 56-57 -0.42574779841314836 1107 58-59 -0.7215084207053195 1107 60-61 -0.5226806429529347 1107 62-63 -0.5321079224411704 1107 64-65 -0.45259012692705625 1107 66-67 -0.47631338956403724 1107 68-69 -0.27320494893439573 1107 70-71 -0.23557949872880357 1107 72-73 -0.2527693263905455 1107 74-75 -0.3989765721934688 1107 76-77 -0.15146701081321012 1107 78-79 -0.10773502005475422 1107 80-81 -0.24070038371379 1107 82-83 -0.01556997132943394 1107 84-85 -0.048922733066000035 1107 86-87 -0.15652927048520127 1107 88-89 -0.0523899327589703 1107 90-91 -0.26811513133246834 1107 92-93 -0.4993011139292136 1107 94-95 -0.319303246865946 1107 96-97 -0.38401472330807707 1107 98-99 -0.25258729507692834 1107 100-101 0.4082394226376742 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96-97 0.225444699088456 2316 98-99 0.2523769132552651 2316 100-101 -0.17467279863985397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 11.0 5 18.0 6 56.0 7 102.0 8 233.0 9 459.0 10 833.0 11 1610.0 12 2675.0 13 3917.0 14 4929.0 15 7607.0 16 11181.0 17 17060.0 18 14575.0 19 6888.0 20 4943.0 21 4750.0 22 4694.0 23 5007.0 24 5561.0 25 6167.0 26 7373.0 27 8755.0 28 10729.0 29 12846.0 30 15833.0 31 19645.0 32 25256.0 33 32889.0 34 46322.0 35 68220.0 36 101048.0 37 159360.0 38 153225.0 39 18389.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1657049103241 20.51237164514002 15.807900883725448 18.51402256081043 2 15.333757249346888 22.54301087625483 40.55063114965512 21.57260072474316 3 19.00714272945731 29.40866269819989 31.076235932244945 20.507958640097858 4 11.813977481377064 18.434600740065427 38.07187164027406 31.679550138283446 5 11.577057590967884 40.79442158125852 32.56763296435854 15.060887863415056 6 27.478312609969173 38.67212139320253 18.023580966681735 15.825985030146558 7 25.871645381486108 37.06417730925695 20.362780174009863 16.701397135247085 8 27.54675206276 33.59611018751899 19.45289297142142 19.40424477829959 9 28.837780620909275 16.10702091744619 20.65952138349843 34.39567707814611 10-11 24.42587753543755 29.10038452526803 25.721056700718297 20.752681238576127 12-13 26.77189032920011 27.07726922440911 27.53058098606162 18.620259460329155 14-15 23.502248542469488 26.737098013979015 28.691133771039386 21.06951967251211 16-17 19.497136013197576 30.664769259449372 28.83452463947986 21.003570087873193 18-19 19.54463503640315 27.108088368612847 34.60297456248548 18.744302032498524 20-21 21.47319752698634 25.492674680211547 34.71348640276618 18.32064139003593 22-23 20.63313605689733 26.158919257116953 34.56889672035906 18.639047965626656 24-25 19.854169220397154 26.27967434828798 35.11902937811555 18.747127053199314 26-27 19.759451817263056 26.458831708844638 35.06878140683467 18.712935067057643 28-29 19.28857363578903 26.674115266010762 35.09069201744249 18.946619080757714 30-31 19.538708660049977 26.439245069084528 35.10255103930049 18.91949523156501 32-33 19.364023831230355 26.629778312354595 34.782819654380766 19.223378202034286 34-35 19.582876857505486 26.655826163789907 34.83657526935266 18.924721709351942 36-37 19.812468118494817 26.56007896079221 34.50416797810447 19.1232849426085 38-39 19.710918446825083 26.842064941200505 34.46180267374899 18.985213938225417 40-41 19.659334310129633 27.112545448161164 34.20076675317379 19.02735348853541 42-43 19.62556014011644 27.317597690096456 33.617218537972036 19.439623631815063 44-45 19.889334513152303 27.347028937486193 33.14927391335992 19.61436263600159 46-47 20.24562868532408 27.894886704614812 32.18939852446583 19.670086085595283 48-49 20.296639061051565 28.233381087727633 31.41428341594588 20.05569643527492 50-51 20.38225221981322 28.62058750673541 30.973257539193078 20.023902734258286 52-53 20.449287131595277 29.116454325603254 30.34421469558489 20.09004384721658 54-55 20.06354909636939 29.074254252567115 30.33923495928108 20.522961691782413 56-57 20.42240932410933 29.3284123323808 29.808446143513446 20.440732199996425 58-59 20.566438620281062 28.72803489390608 30.216337621013977 20.489188864798884 60-61 20.38059230771195 29.01009226557572 30.661194064154323 19.948121362558002 62-63 20.55137172582338 28.8566780817545 30.58898788774908 20.002962304673037 64-65 20.40485256149974 28.961061015815133 30.52891183823774 20.10517458444739 66-67 20.408491599567913 29.20034372949051 30.086864477114922 20.30430019382666 68-69 20.615406027268527 29.103877299297476 30.026916113149827 20.253800560284176 70-71 20.486060568915722 29.381274148528934 29.85607285226527 20.276592430290076 72-73 20.457969748740382 29.33537119465151 29.719002418364248 20.487656638243866 74-75 20.281316795501382 29.869160620756052 29.36939939272754 20.480123191015025 76-77 20.070891015278853 29.501936989736638 29.831876441250603 20.59529555373391 78-79 20.13869204033842 29.446266091570966 29.62764341002127 20.787398458069344 80-81 19.991943031743943 29.863133688214482 29.455880118441264 20.68904316160031 82-83 20.046311843293083 29.81604225907818 29.279504168321267 20.858141729307466 84-85 19.914897148767192 29.551566071223363 29.134415261054432 21.399121518955017 86-87 19.69198415677742 29.467525735021848 29.111410746526317 21.729079361674415 88-89 19.813796167901383 29.090406474167935 29.24241611698039 21.853381240950288 90-91 19.704470925956194 29.04907904952599 29.368512994442632 21.877937030075188 92-93 19.70501124627066 28.969665395515666 29.50187608097315 21.823447277240522 94-95 19.284922635327526 28.93667307648109 29.58646482135515 22.19193946683623 96-97 19.345828640889508 28.811796612247086 29.45392722434606 22.388447522517346 98-99 19.268387357087953 28.85380515696384 29.264378030935656 22.61342945501255 100-101 19.220926490644683 28.901497157393074 29.484001716290976 22.393574635671268 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1516.0 1 1052.0 2 771.5 3 969.5 4 818.0 5 776.0 6 1278.5 7 2550.5 8 3898.5 9 4348.0 10 3815.5 11 3143.5 12 3057.5 13 3112.5 14 3188.5 15 3241.5 16 3218.0 17 3300.5 18 3379.5 19 3456.0 20 3692.5 21 4106.0 22 4569.0 23 5034.5 24 5652.5 25 6517.5 26 7583.0 27 8816.0 28 9854.5 29 11198.0 30 12792.0 31 13937.5 32 15488.0 33 17365.5 34 19107.0 35 20493.0 36 22532.5 37 24559.5 38 25051.0 39 24869.5 40 24605.0 41 24603.0 42 24449.0 43 24618.5 44 25685.0 45 26322.0 46 25865.5 47 26510.5 48 29787.0 49 28937.5 50 24792.0 51 21728.0 52 18997.5 53 17800.5 54 16569.5 55 15137.5 56 14119.5 57 13106.5 58 12287.5 59 11462.5 60 10138.0 61 8705.0 62 7027.0 63 4780.0 64 2985.0 65 2200.5 66 1613.5 67 1157.0 68 832.0 69 585.5 70 456.5 71 360.0 72 323.5 73 335.0 74 262.5 75 207.5 76 165.5 77 98.0 78 60.0 79 41.0 80 32.5 81 27.5 82 20.0 83 10.5 84 5.5 85 5.5 86 2.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07380224573338748 2 0.0 3 0.0 4 0.0 5 0.0016599121012699604 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.38427731257677E-5 12-13 0.006192748993199468 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011491699162638187 24-25 7.661132775092125E-4 26-27 5.745849581319094E-4 28-29 0.0037028808412945275 30-31 3.8305663875460623E-4 32-33 0.0 34-35 0.0 36-37 6.38427731257677E-5 38-39 5.107421850061416E-4 40-41 1.9152831937730311E-4 42-43 0.014173095633920432 44-45 3.8305663875460623E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.276855462515354E-4 82-83 6.384277312576771E-4 84-85 5.107421850061416E-4 86-87 0.0 88-89 0.0 90-91 0.00893798823760748 92-93 0.0032559814294141533 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.011491699162638187 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 783174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.852549939657116 #Duplication Level Percentage of deduplicated Percentage of total 1 77.71307997918224 42.62760600522661 2 12.657780749857467 13.886231014135742 3 4.044426242973418 6.655412774098776 4 1.8764773140171145 4.117182623110296 5 0.9780188073803077 2.682341273687611 6 0.5797946720811968 1.908192972304858 7 0.38969572348987097 1.496306289379927 8 0.2875343486860282 1.2617593776533713 9 0.21005460819054997 1.0369827797244524 >10 1.1353115362687345 11.350964699320159 >50 0.07749192092843865 2.938449859084257 >100 0.045417743290966525 4.453995976046247 >500 0.0030434570237136494 1.1070781715654834 >1k 0.0011705603937360188 1.11118749780259 >5k 4.682241574944076E-4 1.9821078271793569 >10k+ 2.341120787472038E-4 1.3842008596803557 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10779 1.3763225030453003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9137 1.166662836100279 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6298 0.80416357029217 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2435 0.31091430512248874 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2063 0.26341528191691754 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1673 0.21361791887881876 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1386 0.1769721671046281 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1096 0.1399433586916828 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 984 0.12564257751151084 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 0.10572363229627132 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 813 0.1038083491024983 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.2768554625153543E-4 0.0 22-23 0.0 0.0 0.0 1.2768554625153543E-4 0.0 24-25 0.0 0.0 0.0 6.384277312576771E-4 0.0 26-27 0.0 0.0 0.0 0.001340698235641122 0.0 28-29 0.0 0.0 0.0 0.002745239244408011 0.0 30-31 0.0 0.0 0.0 0.004979736303809882 0.0 32-33 0.0 0.0 0.0 0.007916503867595195 0.0 34-35 0.0 0.0 0.0 0.01002331538074553 0.0 36-37 0.0 0.0 0.0 0.01308776849078238 0.0 38-39 0.0 0.0 0.0 0.016599121012699603 0.0 40-41 0.0 0.0 0.0 0.025026367065300942 0.0 42-43 0.0 0.0 0.0 0.0349858396729207 0.0 44-45 0.0 0.0 0.0 0.057905395225071314 0.0 46-47 0.0 0.0 0.0 0.1075750727169186 0.0 48-49 0.0 0.0 0.0 0.14549767995362461 0.0 50-51 0.0 0.0 0.0 0.19657189845423878 0.0 52-53 0.0 0.0 0.0 0.2540942370405555 0.0 54-55 0.0 0.0 0.0 0.3155748275606698 0.0 56-57 0.0 0.0 0.0 0.38880248833592534 0.0 58-59 0.0 0.0 0.0 0.5035917944160557 0.0 60-61 0.0 0.0 0.0 0.6506855436978245 0.0 62-63 0.0 0.0 0.0 0.820954219624247 0.0 64-65 0.0 0.0 0.0 0.9575139113402642 0.0 66-67 0.0 0.0 0.0 1.146360834246285 0.0 68-69 0.0 0.0 0.0 1.389282585989831 0.0 70-71 0.0 0.0 0.0 1.5921749189835208 0.0 72-73 0.0 0.0 0.0 1.91560240763866 0.0 74-75 0.0 0.0 0.0 2.1760170792186666 0.0 76-77 0.0 0.0 0.0 2.416576648356559 0.0 78-79 0.0 0.0 0.0 2.7309384632278393 0.0 80-81 0.0 0.0 0.0 3.0377668308702788 0.0 82-83 0.0 0.0 0.0 3.4205042557592567 0.0 84-85 0.0 0.0 0.0 3.8753201715072256 0.0 86-87 0.0 0.0 0.0 4.433050637533933 0.0 88-89 0.0 0.0 0.0 5.120062719140319 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7245 0.0 33.5894 1 TACGGTG 60 0.003950204 31.666645 5 CTTACGG 60 0.003950204 31.666645 3 TTTCGCG 30 0.003952635 31.664623 82-83 GTATCAA 10740 0.0 30.669004 1 TCTTACG 65 0.0058429693 29.230751 2 TAACGCA 70 0.008389993 27.14284 4 ACGGTGT 70 0.008392606 27.141108 6 GTACACG 90 8.911728E-4 26.40573 1 ACCGTGC 95 0.0012252977 25.003176 8 GTACATG 9000 0.0 24.97982 1 TACATGG 9100 0.0 24.532951 2 ATCAACG 13580 0.0 24.239674 3 TATCAAC 13600 0.0 24.20403 2 TCAACGC 13735 0.0 24.035294 4 CAACGCA 13860 0.0 23.852797 5 GAGTACT 7885 0.0 23.647583 12-13 ACATGGG 9035 0.0 23.50026 3 AACGCAG 14210 0.0 23.464355 6 CATGGGG 1755 0.0 23.005682 4 >>END_MODULE