FastQCFastQC Report
Thu 26 May 2016
SRR937459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067360
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134951.2643344326187977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99810.9351109278968671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69710.6531067306250937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.20517913356318396No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20340.19056363363813522No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.18222530355269076No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18450.1728563933443262No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13560.1270424224254235No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12240.11467546095038225No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11330.1061497526607705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121950.035.356751
GGTATCA77150.035.2457661
GAACGTA600.003947812631.6715989
CCGTTGC1303.0323827E-729.2353239
ATCAACG151050.028.3062933
TATCAAC152200.028.1236292
TCAACGC152500.028.0112614
CAACGCA154950.027.5109065
AACGCAG158150.027.0642766
GTACATG90050.025.8677851
TACATGG91550.025.323442
GAGTACT92750.025.1203212-13
ACGCAGA174250.024.5102737
CGCAGAG175050.024.3439798
GTATAGG2950.024.1721971
ACATGGG91850.024.154553
ATGGGAG23050.023.7099695
GCAGAGT177200.023.5660299
AGTACTT93650.023.56014612-13
GTACTTT99500.023.36624114-15
Produced by FastQC (version 0.11.5)