##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937459_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1067360 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775362576825064 33.0 31.0 34.0 30.0 34.0 2 31.853375618348075 33.0 31.0 34.0 30.0 34.0 3 31.857536351371607 33.0 31.0 34.0 30.0 34.0 4 35.51425479688203 37.0 35.0 37.0 33.0 37.0 5 35.54838761055314 37.0 35.0 37.0 33.0 37.0 6 35.61165398740818 37.0 35.0 37.0 33.0 37.0 7 35.55959938539949 37.0 35.0 37.0 33.0 37.0 8 35.55641770349273 37.0 35.0 37.0 33.0 37.0 9 37.131515140158896 39.0 37.0 39.0 34.0 39.0 10-11 37.02586990331285 39.0 37.0 39.0 33.0 39.0 12-13 36.88135399490331 39.0 37.0 39.0 33.0 39.0 14-15 37.89798334207765 40.0 37.5 41.0 33.0 41.0 16-17 37.85806616324389 40.0 37.5 41.0 33.0 41.0 18-19 37.888773234897315 40.0 38.0 41.0 33.0 41.0 20-21 37.823915548643384 40.0 38.0 41.0 33.0 41.0 22-23 37.872504590766 40.0 38.0 41.0 33.0 41.0 24-25 37.826784308949186 40.0 38.0 41.0 33.0 41.0 26-27 37.71947702743217 40.0 37.0 41.0 33.0 41.0 28-29 37.55690676060561 40.0 37.0 41.0 33.0 41.0 30-31 37.19635549392895 39.0 36.5 41.0 32.0 41.0 32-33 36.84140402488383 39.0 35.0 41.0 31.0 41.0 34-35 36.73535592489881 39.0 35.0 41.0 31.0 41.0 36-37 36.54228563933443 39.0 35.0 41.0 30.5 41.0 38-39 36.3364567718483 39.0 35.0 41.0 30.0 41.0 40-41 36.01972061909758 39.0 35.0 40.5 30.0 41.0 42-43 35.63594569779643 38.5 35.0 40.0 28.5 41.0 44-45 35.20239516189477 38.0 35.0 40.0 26.5 41.0 46-47 34.54827893119472 38.0 34.5 40.0 23.5 41.0 48-49 34.016822815170144 38.0 34.0 40.0 19.5 41.0 50-51 32.98413328211662 37.5 32.0 39.5 15.5 40.5 52-53 33.048649471593464 37.5 33.0 39.5 14.0 40.0 54-55 33.30498191800329 38.0 33.0 40.0 10.5 41.0 56-57 33.0314476840054 38.0 33.0 40.0 8.0 41.0 58-59 32.746537250786986 37.0 33.0 40.0 6.5 41.0 60-61 32.39462505621346 37.0 32.0 40.0 2.0 41.0 62-63 32.01029081097287 36.0 31.5 39.5 2.0 41.0 64-65 31.561173830760005 36.0 31.0 39.0 2.0 41.0 66-67 31.112872414180785 35.0 31.0 39.0 2.0 40.0 68-69 30.605978301603955 35.0 30.0 38.0 2.0 40.0 70-71 30.120122076900014 35.0 29.0 38.0 2.0 40.0 72-73 29.63165286313896 34.5 29.0 37.0 2.0 39.0 74-75 29.081547462899117 34.0 28.5 36.5 2.0 39.0 76-77 28.551126143007046 34.0 27.5 36.0 2.0 39.0 78-79 28.0044155673812 34.0 26.0 35.5 2.0 37.5 80-81 27.399308105981113 33.5 26.0 35.0 2.0 37.0 82-83 26.82760736771099 33.0 25.0 35.0 2.0 36.5 84-85 26.264369097586567 33.0 23.5 35.0 2.0 36.0 86-87 25.650601015589867 32.0 20.5 35.0 2.0 36.0 88-89 25.059422781442063 32.0 18.5 35.0 2.0 35.0 90-91 24.46692165717284 31.0 13.0 34.0 2.0 35.0 92-93 23.77925676435317 31.0 4.5 34.0 2.0 35.0 94-95 22.977301004347176 31.0 2.0 34.0 2.0 35.0 96-97 22.10369462974067 30.0 2.0 34.0 2.0 35.0 98-99 20.933709338929695 29.5 2.0 34.0 2.0 35.0 100-101 18.023509874831362 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2949691811805799 1101 2 0.3962535859595633 1101 3 0.407683333343261 1101 4 0.11393777340535394 1101 5 0.1797122492488583 1101 6 0.16333348870189468 1101 7 0.3436061980228331 1101 8 0.2879219445368406 1101 9 0.4132290878865632 1101 10-11 0.3265967820285596 1101 12-13 0.4727914985147237 1101 14-15 0.5868984686568695 1101 16-17 0.40896644564480766 1101 18-19 0.5575809733113388 1101 20-21 0.4891965435756589 1101 22-23 0.3694505395757872 1101 24-25 0.3637683929995603 1101 26-27 0.4089344972544069 1101 28-29 0.4114788191911103 1101 30-31 0.5338794849228563 1101 32-33 0.620181811109731 1101 34-35 0.7087764607438629 1101 36-37 0.5235014702962744 1101 38-39 0.4453100669011434 1101 40-41 0.7524110203670276 1101 42-43 0.8018978149583873 1101 44-45 0.7752651616472761 1101 46-47 0.9743114984778458 1101 48-49 1.244810243227171 1101 50-51 1.2579307473993637 1101 52-53 1.1245112524048295 1101 54-55 1.029060101828975 1101 56-57 1.0326206232009696 1101 58-59 1.032571387457466 1101 60-61 1.061238208419958 1101 62-63 1.175640493520202 1101 64-65 1.073201296780173 1101 66-67 1.1890784550737727 1101 68-69 1.4076899310864128 1101 70-71 1.4421975365895179 1101 72-73 1.558713580270684 1101 74-75 1.6392938623914297 1101 76-77 1.5680631648034158 1101 78-79 1.5167860890071658 1101 80-81 1.7948470544647002 1101 82-83 1.7418667503226537 1101 84-85 2.1134144533606474 1101 86-87 2.0926347164120394 1101 88-89 1.875530613183539 1101 90-91 1.8357054248708948 1101 92-93 2.3331502163658513 1101 94-95 2.3340509547730157 1101 96-97 2.268183141781684 1101 98-99 2.4350673394114786 1101 100-101 2.6142922477113366 1102 1 0.30667777336579505 1102 2 0.22088440376177942 1102 3 0.17767518587406883 1102 4 0.17475693353706845 1102 5 0.2510323089969617 1102 6 0.14623144165025792 1102 7 0.12508004125194816 1102 8 0.024269841471351583 1102 9 0.22540531085563487 1102 10-11 0.3536527449575715 1102 12-13 0.39148612118505355 1102 14-15 0.5216915568871769 1102 16-17 0.5980564751793835 1102 18-19 0.6175849621764655 1102 20-21 0.3211957288287337 1102 22-23 0.3350694926259976 1102 24-25 0.26816038811107745 1102 26-27 0.36907979378834455 1102 28-29 0.2718562337275543 1102 30-31 0.6316397795896549 1102 32-33 0.5253927966140282 1102 34-35 0.5711894695363355 1102 36-37 0.6267655501414708 1102 38-39 0.5226113026006374 1102 40-41 0.7419928684846226 1102 42-43 1.1185343529629321 1102 44-45 1.1383847087837822 1102 46-47 0.8766373463238608 1102 48-49 1.010604451734487 1102 50-51 0.7660934930964842 1102 52-53 0.8922383631085609 1102 54-55 0.840971532049295 1102 56-57 0.8412263875354142 1102 58-59 0.90270030418354 1102 60-61 0.9211878808238012 1102 62-63 1.0701774456877722 1102 64-65 0.88254457681548 1102 66-67 0.82895055678134 1102 68-69 1.0692096886992069 1102 70-71 1.1074761630620067 1102 72-73 1.0832209129929566 1102 74-75 1.1297753268990185 1102 76-77 0.8594582491636693 1102 78-79 0.9998355339608267 1102 80-81 1.1483716835851112 1102 82-83 1.1760521731423559 1102 84-85 1.2509055420662172 1102 86-87 1.2704154646212977 1102 88-89 1.4290089884423907 1102 90-91 1.460482642510165 1102 92-93 1.7130569448174953 1102 94-95 1.568551243329214 1102 96-97 1.2822842043474587 1102 98-99 1.8050935640779429 1102 100-101 1.9523307445032714 1103 1 0.34167325665395865 1103 2 0.32952939021164696 1103 3 0.3574854839770545 1103 4 0.3433115857502571 1103 5 0.34571613916860144 1103 6 0.41814201075595037 1103 7 0.26160668985176727 1103 8 0.1596627954008909 1103 9 0.49606023407153543 1103 10-11 0.26300911352125667 1103 12-13 0.4104901862257009 1103 14-15 0.480517211810394 1103 16-17 0.3887746323609562 1103 18-19 0.3905818004781807 1103 20-21 0.2866157830292835 1103 22-23 0.2374610915419808 1103 24-25 0.4320221767289638 1103 26-27 0.3769750060737991 1103 28-29 0.29610871791906135 1103 30-31 0.28313481120664363 1103 32-33 0.5014180902002892 1103 34-35 0.541885043158743 1103 36-37 0.6080392628786058 1103 38-39 0.630102269176966 1103 40-41 0.715328460173879 1103 42-43 0.4182927088798323 1103 44-45 0.6030843474468313 1103 46-47 0.7451129560799643 1103 48-49 0.48173362969293265 1103 50-51 0.5534376665382226 1103 52-53 0.25658118153674536 1103 54-55 0.3458269972706134 1103 56-57 0.48848926016414396 1103 58-59 0.3432400512476761 1103 60-61 0.26413955200718675 1103 62-63 0.37378629844296185 1103 64-65 0.420073935442403 1103 66-67 0.6435869614787215 1103 68-69 0.4980782523848397 1103 70-71 0.527080950776547 1103 72-73 0.6268184739685694 1103 74-75 0.45277668191256737 1103 76-77 0.3034194054419004 1103 78-79 0.35986940929958067 1103 80-81 0.7415198317332603 1103 82-83 0.6662780087340465 1103 84-85 0.868480067811479 1103 86-87 1.018019348993473 1103 88-89 1.1628865855516963 1103 90-91 1.1460043227269665 1103 92-93 1.3670971435528116 1103 94-95 1.500452779011237 1103 96-97 1.3685488836609174 1103 98-99 1.4397209353516587 1103 100-101 1.2006234274300986 1104 1 0.17233750647613988 1104 2 0.15695109133364227 1104 3 0.09497946098643695 1104 4 0.08855262009851117 1104 5 0.12743469915758254 1104 6 0.20351428893781076 1104 7 0.26875534873251894 1104 8 0.3251374070614048 1104 9 0.12880327608333175 1104 10-11 0.24096198505373678 1104 12-13 0.16460073391632335 1104 14-15 0.3408232944572944 1104 16-17 0.3143442351877468 1104 18-19 0.3734127207911513 1104 20-21 0.32744638211607935 1104 22-23 0.35091955011527887 1104 24-25 0.38560316013992946 1104 26-27 0.3964307571694121 1104 28-29 0.391963982586482 1104 30-31 0.29641017086522226 1104 32-33 0.5508309346579949 1104 34-35 0.7494306458019793 1104 36-37 0.8725740585558128 1104 38-39 0.8891730077677025 1104 40-41 1.0066191010200782 1104 42-43 1.0757444675930827 1104 44-45 0.9098957188531145 1104 46-47 1.0744854178196306 1104 48-49 1.0364049464907694 1104 50-51 0.8890212656479619 1104 52-53 0.9162728044172326 1104 54-55 1.1621911759641463 1104 56-57 1.188033119008189 1104 58-59 1.1632648315519276 1104 60-61 1.22919152181192 1104 62-63 1.0529001731188679 1104 64-65 1.166551231635875 1104 66-67 1.331833296058722 1104 68-69 1.1262273013259438 1104 70-71 1.0731755289377567 1104 72-73 1.4199212892864672 1104 74-75 1.3162509723469107 1104 76-77 1.3639485108270257 1104 78-79 1.3619853719455648 1104 80-81 1.6457907630596935 1104 82-83 1.7103750190362312 1104 84-85 1.7421436522365958 1104 86-87 1.833513004776691 1104 88-89 1.5686535130234134 1104 90-91 1.9335214739097673 1104 92-93 2.031378170558753 1104 94-95 2.094359577533595 1104 96-97 2.3339418120963167 1104 98-99 2.5607915362740314 1104 100-101 2.218652475453414 1105 1 0.18525908941292712 1105 2 0.08867028444647573 1105 3 0.2368415993248938 1105 4 0.18900960918445975 1105 5 0.185749044875962 1105 6 0.2119637418629594 1105 7 0.29123605597222735 1105 8 0.24776827153313974 1105 9 0.350006917932177 1105 10-11 0.3443457967148902 1105 12-13 0.3296622074810216 1105 14-15 0.3815484055316247 1105 16-17 0.12320334036726877 1105 18-19 0.33413472752978635 1105 20-21 0.3069346126533148 1105 22-23 0.39582736763865256 1105 24-25 0.2865362617057272 1105 26-27 0.33984180465974134 1105 28-29 0.29357497845222014 1105 30-31 0.3890227094657561 1105 32-33 0.3913538351365986 1105 34-35 0.6377616022889612 1105 36-37 0.6579465488247536 1105 38-39 0.6446212455426661 1105 40-41 0.6769995365017749 1105 42-43 0.5711192646750121 1105 44-45 0.6925176133382891 1105 46-47 0.9356049853393529 1105 48-49 1.0011004171620854 1105 50-51 0.9797013124016587 1105 52-53 0.8046826556235089 1105 54-55 1.0857696346185932 1105 56-57 1.0844756532094806 1105 58-59 1.2700839937071535 1105 60-61 1.3911898221452574 1105 62-63 1.1534643561168814 1105 64-65 1.3562008447516014 1105 66-67 1.4104780391406386 1105 68-69 1.2628197869469524 1105 70-71 1.2646881722452967 1105 72-73 1.5787427908416376 1105 74-75 1.6400546239718423 1105 76-77 1.5278510038144013 1105 78-79 1.6411616253211037 1105 80-81 1.5401160031097412 1105 82-83 1.7152634902023536 1105 84-85 1.7097149211809786 1105 86-87 1.8382616950814743 1105 88-89 1.892598660612279 1105 90-91 1.6196984330500221 1105 92-93 1.8403354653617399 1105 94-95 1.6903067043508564 1105 96-97 1.967414526100388 1105 98-99 1.980292338513273 1105 100-101 1.6746643816956137 1106 1 0.12202168870971164 1106 2 -0.5513378184604427 1106 3 -0.1262930680941814 1106 4 -0.01439650561636796 1106 5 -0.0483063153416623 1106 6 0.025993346412164442 1106 7 -0.13087233970043144 1106 8 0.025328042529551453 1106 9 0.19484975530008342 1106 10-11 -0.25395307514824594 1106 12-13 -0.186490069291132 1106 14-15 0.12645346164907778 1106 16-17 0.10586070798361646 1106 18-19 0.07214845423995797 1106 20-21 0.02701583464884294 1106 22-23 0.08169647675297398 1106 24-25 0.2122080071586936 1106 26-27 0.20294222765077308 1106 28-29 0.30102290039422286 1106 30-31 0.16960274255261965 1106 32-33 0.186768457989686 1106 34-35 -0.06108566273879035 1106 36-37 0.08099835437427316 1106 38-39 0.2505874930768286 1106 40-41 0.22526006425182032 1106 42-43 -0.09740480047621958 1106 44-45 0.08778947068854137 1106 46-47 -0.01681503462852163 1106 48-49 -0.3370145958845612 1106 50-51 -0.2955467185437257 1106 52-53 -0.48441507604487555 1106 54-55 -0.23402846795070076 1106 56-57 -0.48734504103600784 1106 58-59 -0.501730912747675 1106 60-61 -0.3750422779063527 1106 62-63 -0.34820879769847224 1106 64-65 -0.32129140202049555 1106 66-67 -0.27138013104934444 1106 68-69 -0.1786077716182568 1106 70-71 -0.3669285988427582 1106 72-73 -0.02292988065783419 1106 74-75 -0.3700262604025717 1106 76-77 -0.005753391047971235 1106 78-79 0.22715034877564477 1106 80-81 -0.0356230254095955 1106 82-83 -0.11124941415922862 1106 84-85 -0.2129833468226714 1106 86-87 -0.29856601686018536 1106 88-89 0.05142962336302759 1106 90-91 -0.0670967225692003 1106 92-93 0.20849345275399855 1106 94-95 -0.07999373021575806 1106 96-97 0.23426833133158453 1106 98-99 0.24365176513614273 1106 100-101 0.2301085222810464 1107 1 0.16957531271083326 1107 2 0.24613246831308544 1107 3 0.22413140656974306 1107 4 0.28445235024916826 1107 5 0.20280510731048906 1107 6 0.14550360390082773 1107 7 0.21838593301220754 1107 8 0.15404849156404055 1107 9 0.2711845788380458 1107 10-11 0.27394905942709613 1107 12-13 0.2923734290031774 1107 14-15 0.36141096225918545 1107 16-17 0.5132701923312766 1107 18-19 0.389716772593367 1107 20-21 0.3609296727825111 1107 22-23 0.48650199726017007 1107 24-25 0.4527613040417293 1107 26-27 0.465888575091725 1107 28-29 0.36642107580220085 1107 30-31 0.497956611632695 1107 32-33 0.5498103446822142 1107 34-35 0.4593550639825992 1107 36-37 0.6752826148018158 1107 38-39 0.725258687473243 1107 40-41 0.753114429201375 1107 42-43 0.5452430906505725 1107 44-45 0.5836937445123453 1107 46-47 0.5876898487772451 1107 48-49 0.521253962874674 1107 50-51 0.16824702051987828 1107 52-53 0.11638921906756394 1107 54-55 0.3047968146429767 1107 56-57 0.47214995094181234 1107 58-59 0.5098864525497646 1107 60-61 0.38510175790009527 1107 62-63 0.18194733628494575 1107 64-65 -0.02729531705287158 1107 66-67 0.36081140438065873 1107 68-69 0.3773372102344794 1107 70-71 0.4414451050167152 1107 72-73 0.49918018679080234 1107 74-75 0.5249516980690068 1107 76-77 0.3495674702591529 1107 78-79 0.5042448132106401 1107 80-81 0.564503148710692 1107 82-83 0.5698405640848208 1107 84-85 0.4988910398309123 1107 86-87 0.48278825203088616 1107 88-89 1.005379704104076 1107 90-91 0.8754660140072765 1107 92-93 1.123055400093179 1107 94-95 1.203775500952883 1107 96-97 1.4578966421041564 1107 98-99 1.7114737298177758 1107 100-101 1.3422227955336936 1108 1 0.27738721520200116 1108 2 0.3874728231671014 1108 3 0.26836376177391585 1108 4 0.25271728825799045 1108 5 0.32921436429169404 1108 6 0.254095291415517 1108 7 0.21930278512257928 1108 8 0.16832304960907152 1108 9 0.4456583103339895 1108 10-11 0.3803937465225147 1108 12-13 0.35093447799256694 1108 14-15 0.5696440868819579 1108 16-17 0.44715663167390574 1108 18-19 0.3890181911801207 1108 20-21 0.4467434596581583 1108 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46-47 -0.5334377114128586 2316 48-49 -0.464932285926956 2316 50-51 -0.30798913456582255 2316 52-53 -0.2782613802837943 2316 54-55 -0.40297038908662586 2316 56-57 -0.2513704818512892 2316 58-59 -0.2710369723539685 2316 60-61 -0.40860533618072736 2316 62-63 -0.31249939974889074 2316 64-65 -0.12962076404500777 2316 66-67 -0.16522338043531803 2316 68-69 -0.07863159947118348 2316 70-71 0.15731471408415487 2316 72-73 -0.038516962295723545 2316 74-75 0.17388297380689366 2316 76-77 0.21728692079141965 2316 78-79 0.41459368125361706 2316 80-81 0.4588177532113207 2316 82-83 0.6329078741089127 2316 84-85 0.6687718494821802 2316 86-87 0.9321499184746891 2316 88-89 0.701873798980408 2316 90-91 0.4321114595489135 2316 92-93 0.5925807237927714 2316 94-95 0.681948300894863 2316 96-97 0.585583751007281 2316 98-99 1.1963913572521818 2316 100-101 1.5917071879212834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4395.0 3 674.0 4 598.0 5 698.0 6 874.0 7 1047.0 8 1365.0 9 1721.0 10 2377.0 11 3374.0 12 5186.0 13 7324.0 14 8448.0 15 11517.0 16 15629.0 17 20958.0 18 16688.0 19 9941.0 20 9380.0 21 9759.0 22 10580.0 23 11851.0 24 13262.0 25 14744.0 26 16774.0 27 19375.0 28 22864.0 29 26951.0 30 32262.0 31 39070.0 32 48564.0 33 61940.0 34 81144.0 35 110042.0 36 141036.0 37 166518.0 38 108656.0 39 9774.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08322844985191 19.209515367819087 15.439443771331138 20.26781241099787 2 15.015248055708486 21.408345374568537 40.86112525577838 22.715281313944597 3 18.665382718369074 27.647978955774242 31.126476736266056 22.560161589590624 4 11.784308383205405 17.27186463608264 38.285590807880574 32.65823617283139 5 12.117716029432765 39.08945200640366 33.41222030981067 15.380611654352908 6 28.995010316520425 37.177039379337096 18.12026142072151 15.707688883420973 7 26.241808151558356 35.99107043909623 20.866415346673204 16.900706062672217 8 26.998780961969395 32.897027913904736 20.206071898825478 19.89811922530039 9 27.844722241268705 16.190155046696226 20.722173640652723 35.24294907138235 10-11 24.133499790050358 27.770936018434234 26.794693675490187 21.300870516025217 12-13 26.385144099696845 25.765913429814653 28.10856379343949 19.740378677049016 14-15 23.208959148865834 25.712565649583375 28.471069102056457 22.60740609949434 16-17 19.946703481178346 29.69686724471085 28.598865042414168 21.757564231696637 18-19 19.809101259434016 26.595678251581628 33.68339260772093 19.911827881263424 20-21 21.331557847743348 25.369034617273613 33.56818136998497 19.73122616499808 22-23 20.794030730918067 25.86327999917365 33.62460689572278 19.718082374185503 24-25 20.321212163897556 25.87328529941378 34.05722281305978 19.748279723628887 26-27 20.095302217614712 26.199893326121575 33.69537658883981 20.009427867423902 28-29 19.72849841563813 26.56435777464307 33.89999079623811 19.807153013480693 30-31 19.93171256343241 26.282585059892405 33.80650797117274 19.979194405502447 32-33 19.658248498902527 26.493231560192765 33.450218414025834 20.398301526878875 34-35 20.15510938193396 26.280544643842468 33.55124409182588 20.01310188239769 36-37 20.44269586105993 26.121178143271145 33.24092699335689 20.195199002312037 38-39 20.153050924643544 26.393960591734313 33.17078323575945 20.282205247862688 40-41 19.985202301718118 26.677780589382184 33.06576666114257 20.27125044775713 42-43 19.984345852425616 26.929594367887493 32.47474982240008 20.611309957286807 44-45 19.991436105080492 26.995659833079173 32.17611199170655 20.836792070133793 46-47 20.497130943548612 27.39070820146317 31.356157436537973 20.756003418450238 48-49 20.49980206835052 27.674131718699446 30.683256297302258 21.142809915647774 50-51 20.655757107236838 28.03128682422484 30.328578345982045 20.98437772255628 52-53 20.744641372409937 28.215012142934313 29.967540550824168 21.072805933831575 54-55 20.260813622040956 28.128425115872812 30.035830175300188 21.574931086786037 56-57 20.806351886115248 28.36625879748152 29.535923020188136 21.291466296215095 58-59 21.087838532461515 27.632107051671134 29.83423158007399 21.44582283579336 60-61 20.640757338050268 27.729112505034742 30.5218575925806 21.108272564334396 62-63 20.84341523736018 27.842392728071687 30.550117508757996 20.764074525810145 64-65 20.63561955584931 28.024032656923918 30.43710932145627 20.903238465770503 66-67 20.555827579747817 28.053899285533547 30.278514694705137 21.1117584400135 68-69 20.572558217268103 28.101462637855825 30.23847437989525 21.087504764980817 70-71 20.51139889902813 28.179190073038207 29.99308019555724 21.316330832376423 72-73 20.361130385712155 28.26119207486829 29.86107264015504 21.516604899264518 74-75 20.200231368454265 28.787249945538267 29.69061042209719 21.321908263910277 76-77 20.173822810604975 28.356407192376587 29.901232770905573 21.568537226112863 78-79 20.264972057543336 28.30142167989054 29.817526508005226 21.6160797545609 80-81 20.179649265139478 28.468567951592732 29.800960408051353 21.550822375216438 82-83 20.025773498365098 28.224609700506313 29.868292259265434 21.881324541863158 84-85 20.072006271211105 28.039964888728246 29.709627905011804 22.178400935048842 86-87 20.488206060304496 27.91931047853132 29.524210095437752 22.068273365726434 88-89 20.457227844927875 27.717419370588726 29.322777732395515 22.50257505208788 90-91 20.066458003433695 27.4571175460678 29.797137836509368 22.679286613989138 92-93 20.14220957719764 27.37372385801578 29.81044200464329 22.673624560143292 94-95 19.90523262746836 27.19893073334153 29.92082844692492 22.975008192265186 96-97 19.960814840968162 27.183260340449827 29.702829444552954 23.153095374029057 98-99 19.924032030499873 27.486788944735412 29.430921029464585 23.158257995300126 100-101 20.159973261115812 26.46149642926049 30.504907683720685 22.87362262590301 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2505.0 1 1592.0 2 852.0 3 1030.0 4 873.0 5 856.0 6 1533.0 7 3188.5 8 5223.0 9 6010.5 10 5063.0 11 3984.0 12 3790.5 13 3892.5 14 3904.5 15 3873.5 16 3889.5 17 3975.0 18 4139.5 19 4354.5 20 4509.0 21 4817.5 22 5323.0 23 6138.5 24 7012.5 25 7981.0 26 9238.5 27 10632.5 28 11689.0 29 13010.5 30 15051.5 31 17008.0 32 19019.5 33 20457.5 34 21755.5 35 22886.0 36 24737.5 37 27464.0 38 28989.5 39 29688.5 40 31213.5 41 33172.5 42 33924.0 43 35099.0 44 37638.5 45 39947.0 46 39970.0 47 40295.5 48 43991.0 49 42833.0 50 36965.0 51 32414.5 52 29039.0 53 27796.5 54 26976.5 55 24643.5 56 21968.5 57 19944.0 58 18650.5 59 17087.5 60 14438.0 61 12142.0 62 9987.0 63 7269.5 64 5023.5 65 3726.5 66 2733.5 67 2037.5 68 1544.5 69 1090.0 70 760.5 71 567.0 72 438.5 73 356.5 74 277.0 75 223.0 76 169.0 77 107.5 78 66.5 79 47.0 80 29.5 81 17.5 82 11.5 83 11.0 84 11.0 85 5.5 86 3.5 87 3.5 88 1.5 89 0.0 90 0.5 91 0.5 92 1.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32613176435317043 2 0.27750712037175834 3 0.27497751461549996 4 0.2782566331884275 5 0.2780692549842602 6 0.24012516864038375 7 0.24190526157997302 8 0.2422800179883076 9 0.2541785339529306 10-11 0.26410957877379704 12-13 0.25600547144356167 14-15 0.245090691050817 16-17 0.24007832408934193 18-19 0.2246196222455404 20-21 0.23300479688202666 22-23 0.2285077199820117 24-25 0.2330516414330685 26-27 0.22785189626742616 28-29 0.24153050517163843 30-31 0.25694236246439817 32-33 0.24724554039874083 34-35 0.2461681157247789 36-37 0.23403537700494675 38-39 0.22063783540698548 40-41 0.2182487633038525 42-43 0.23108417028931197 44-45 0.22710238345075698 46-47 0.23890721031329634 48-49 0.24345113176435315 50-51 0.2584413880977365 52-53 0.27685129665717284 54-55 0.24499700194873333 56-57 0.22049730175385998 58-59 0.20887985309548793 60-61 0.21183105981112277 62-63 0.21857667516114523 64-65 0.20855194123819515 66-67 0.21543809024134314 68-69 0.21529755658821767 70-71 0.21557862389446858 72-73 0.21951356618198173 74-75 0.22410433218408035 76-77 0.23258319592265028 78-79 0.23356693149452853 80-81 0.22447908859241492 82-83 0.21665604856843054 84-85 0.20377379703192924 86-87 0.2075682056663169 88-89 0.21857667516114523 90-91 0.21796769599760155 92-93 0.2232142857142857 94-95 0.21838929695697798 96-97 0.22635287063408785 98-99 0.22836718632888625 100-101 0.21028518962674264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1067360.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.48094161894465 #Duplication Level Percentage of deduplicated Percentage of total 1 71.92502790448052 33.43143022969123 2 14.042448883303694 13.054124934637072 3 5.411115260400481 7.545411975361657 4 2.7092165371731687 5.037077427897018 5 1.6106648721358368 3.7432609944715387 6 0.9344744275197692 2.6061150785965865 7 0.669191103395855 2.177324282620192 8 0.461174333417542 1.7148653814189185 9 0.33598418516489414 1.4055175165984322 >10 1.7156765693764782 14.382203613385421 >50 0.11114467627315573 3.5567840202803973 >100 0.06780881630751183 5.940470495448941 >500 0.0036434586306937546 1.241533841547422 >1k 0.0018217293153468773 1.299301481643672 >5k 4.0482873674375046E-4 1.5949137376410953 >10k+ 2.0241436837187523E-4 1.2696649887604166 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13495 1.2643344326187977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9981 0.9351109278968671 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6971 0.6531067306250937 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2190 0.20517913356318396 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2034 0.19056363363813522 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1945 0.18222530355269076 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1845 0.1728563933443262 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1356 0.1270424224254235 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1224 0.11467546095038225 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1133 0.1061497526607705 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.216009593764053E-4 0.0 24-25 0.0 0.0 0.0 9.83735571878279E-4 0.0 26-27 0.0 0.0 0.0 0.002201693898965672 0.0 28-29 0.0 0.0 0.0 0.004450232348973167 0.0 30-31 0.0 0.0 0.0 0.00946259931044821 0.0 32-33 0.0 0.0 0.0 0.017473017538599908 0.0 34-35 0.0 0.0 0.0 0.024874456603207917 0.0 36-37 0.0 0.0 0.0 0.03227589566781592 0.0 38-39 0.0 0.0 0.0 0.040145780242842155 0.0 40-41 0.0 0.0 0.0 0.04853095487932844 0.0 42-43 0.0 0.0 0.0 0.05579186029081097 0.0 44-45 0.0 0.0 0.0 0.0640833458252136 0.0 46-47 0.0 0.0 0.0 0.07748088742317494 0.0 48-49 0.0 0.0 0.0 0.09584395143156949 0.0 50-51 0.0 0.0 0.0 0.11158372058162194 0.0 52-53 0.0 0.0 0.0 0.14067418677859392 0.0 54-55 0.0 0.0 0.0 0.19093839004646979 0.0 56-57 0.0 0.0 0.0 0.29568280617598564 0.0 58-59 0.0 0.0 0.0 0.41546432318992654 0.0 60-61 0.0 0.0 0.0 0.5254084844850847 0.0 62-63 0.0 0.0 0.0 0.6660826712636786 0.0 64-65 0.0 0.0 0.0 0.8275089941538001 0.0 66-67 9.368910208364563E-5 0.0 0.0 1.0803290361265179 0.0 68-69 9.368910208364563E-5 0.0 0.0 1.388285114675461 0.0 70-71 9.368910208364563E-5 0.0 0.0 1.6760511917253784 0.0 72-73 9.368910208364563E-5 0.0 0.0 1.991315020236846 0.0 74-75 9.368910208364563E-5 0.0 0.0 2.313980287812922 0.0 76-77 9.368910208364563E-5 0.0 0.0 2.688127716983961 0.0 78-79 9.368910208364563E-5 0.0 0.0 3.030561385099685 0.0 80-81 9.368910208364563E-5 0.0 0.0 3.359972268025783 0.0 82-83 9.368910208364563E-5 9.368910208364563E-5 0.0 3.6573883225903163 0.0 84-85 9.368910208364563E-5 9.368910208364563E-5 0.0 4.131408334582521 0.0 86-87 9.368910208364563E-5 9.368910208364563E-5 0.0 4.656020461699895 0.0 88-89 9.368910208364563E-5 9.368910208364563E-5 0.0 5.32894243741568 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12195 0.0 35.35675 1 GGTATCA 7715 0.0 35.245766 1 GAACGTA 60 0.0039478126 31.671598 9 CCGTTGC 130 3.0323827E-7 29.235323 9 ATCAACG 15105 0.0 28.306293 3 TATCAAC 15220 0.0 28.123629 2 TCAACGC 15250 0.0 28.011261 4 CAACGCA 15495 0.0 27.510906 5 AACGCAG 15815 0.0 27.064276 6 GTACATG 9005 0.0 25.867785 1 TACATGG 9155 0.0 25.32344 2 GAGTACT 9275 0.0 25.12032 12-13 ACGCAGA 17425 0.0 24.510273 7 CGCAGAG 17505 0.0 24.343979 8 GTATAGG 295 0.0 24.172197 1 ACATGGG 9185 0.0 24.15455 3 ATGGGAG 2305 0.0 23.709969 5 GCAGAGT 17720 0.0 23.566029 9 AGTACTT 9365 0.0 23.560146 12-13 GTACTTT 9950 0.0 23.366241 14-15 >>END_MODULE