FastQCFastQC Report
Thu 26 May 2016
SRR937459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067360
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137991.292815919652226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109551.026364113326338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78610.7364900314795383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29010.27179208514465597No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.22794558536950982No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22900.21454804377154849No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17340.16245690301304153No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.14465597361714885No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA12910.12095263078998651No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12200.11430070454204767No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11980.11223954429620746No Hit
TATTAGGGCATTGTCACCACAATATAGATCTTCCTTGTCCAAGAATGCAA11650.10914780392744716No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.10436965972118123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93550.032.7568131
GTATCAA138600.031.6391051
GCGACAG1457.8849916E-726.207547
ATCAACG173850.025.1906993
TATCAAC175150.025.0037292
TCAACGC175300.024.9823324
CAACGCA176800.024.7972455
AACGCAG179850.024.4031286
GAGTACT102850.024.0161512-13
GTCTAGC1601.8559222E-623.7550371
AGTACTT104450.023.48909212-13
GTACATG94600.023.4537031
TAAGGTG4800.022.7599125
TACATGG95250.022.6897872
GTACTTT109300.022.51093914-15
CTAAGGG3450.022.0284962
ACGCAGA198900.021.971387
CGCAGAG199750.021.8303268
ACATGGG96600.021.0450823
TACTTTT116350.021.00404714-15
Produced by FastQC (version 0.11.5)