FastQCFastQC Report
Thu 26 May 2016
SRR937458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060013
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137451.2966822105011921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99430.9380073640606295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71400.6735766448147334No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21940.20697859365875704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21580.20358240889498525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.18773354666405034No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15160.14301711394105543No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13650.12877200562634608No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.12141360530484059No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10790.10179120444749262No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.10103649672221No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80450.034.6377681
GTATCAA122400.033.4503061
TATCAAC153100.026.7611562
ATCAACG152100.026.7483393
TCAACGC153500.026.4449544
GTACATG87900.026.3462281
CAACGCA155400.026.1216265
TACATGG88050.026.018992
AACGCAG157150.025.8777626
GAGTACT92350.024.20301612-13
ACATGGG90300.024.1062683
CTAAGGG3000.023.7652722
ACGCAGA174050.023.2285927
CGCAGAG174400.023.1002528
AGTACTT94500.022.8731712-13
CATGGGG24750.022.4700494
GTACTTT98900.022.45598414-15
CATGGGA63850.022.1100064
GCAGAGT179350.022.0918469
GTCGGGA3650.020.8353082
Produced by FastQC (version 0.11.5)