##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937458_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1060013 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771449029398696 33.0 31.0 34.0 30.0 34.0 2 31.842442498346717 33.0 31.0 34.0 30.0 34.0 3 31.84972448451104 33.0 31.0 34.0 30.0 34.0 4 35.50401174325221 37.0 35.0 37.0 33.0 37.0 5 35.52970859791342 37.0 35.0 37.0 33.0 37.0 6 35.611203824858755 37.0 35.0 37.0 33.0 37.0 7 35.56110160913121 37.0 35.0 37.0 33.0 37.0 8 35.559766719842116 37.0 35.0 37.0 33.0 37.0 9 37.1616895264492 39.0 37.0 39.0 34.0 39.0 10-11 37.04833902980435 39.0 37.0 39.0 33.0 39.0 12-13 36.916717059130406 39.0 37.0 39.0 33.0 39.0 14-15 38.0057933251762 40.0 38.0 41.0 33.0 41.0 16-17 38.01022204444662 40.0 38.0 41.0 33.0 41.0 18-19 38.08377585935267 40.0 38.0 41.0 33.5 41.0 20-21 38.0805546724427 40.0 38.0 41.0 33.0 41.0 22-23 37.9381634942213 40.0 38.0 41.0 33.0 41.0 24-25 37.77232637712933 40.0 37.5 41.0 33.0 41.0 26-27 37.59919548156485 40.0 37.0 41.0 32.5 41.0 28-29 37.603052509733374 40.0 37.0 41.0 33.0 41.0 30-31 37.33811330615757 39.0 36.5 41.0 32.5 41.0 32-33 37.03466089566826 39.0 35.5 41.0 32.0 41.0 34-35 36.82768230200951 39.0 35.0 41.0 31.0 41.0 36-37 36.73826217225638 39.0 35.0 41.0 31.0 41.0 38-39 36.59973698435774 39.0 35.0 41.0 31.0 41.0 40-41 36.34215948294974 39.0 35.0 41.0 30.5 41.0 42-43 35.97619227311363 39.0 35.0 41.0 30.0 41.0 44-45 35.564881751450216 39.0 35.0 41.0 28.0 41.0 46-47 34.97183289261547 39.0 35.0 41.0 24.5 41.0 48-49 34.464024026120434 39.0 35.0 40.0 22.0 41.0 50-51 33.517162053672926 37.5 33.5 39.5 19.0 40.5 52-53 33.563868084636695 38.0 33.5 39.5 18.0 40.5 54-55 33.85612865125239 38.0 34.0 40.0 16.0 41.0 56-57 33.65051419180708 38.0 34.0 40.0 13.0 41.0 58-59 33.4052087097045 38.0 33.5 40.0 9.0 41.0 60-61 33.10784584717358 37.5 33.0 40.0 7.5 41.0 62-63 32.779088558347866 37.0 33.0 40.0 4.5 41.0 64-65 32.39817389032021 36.5 32.5 39.5 2.0 41.0 66-67 32.01741865429952 36.0 32.0 39.0 2.0 41.0 68-69 31.552412564751563 35.0 31.5 39.0 2.0 40.5 70-71 31.1065746363488 35.0 31.0 38.5 2.0 40.0 72-73 30.72304820789934 35.0 31.0 37.5 2.0 40.0 74-75 30.27934091374351 35.0 30.5 37.0 2.0 39.0 76-77 29.844848600913387 35.0 30.0 36.5 2.0 39.0 78-79 29.38316841397228 34.5 29.0 36.0 2.0 38.5 80-81 28.90359882378801 34.0 29.0 35.5 2.0 37.5 82-83 28.4374352012664 34.0 29.0 35.0 2.0 37.0 84-85 28.00888007977261 34.0 27.5 35.0 2.0 36.5 86-87 27.56696380138734 34.0 26.5 35.0 2.0 36.0 88-89 27.159194274032487 34.0 26.0 35.0 2.0 36.0 90-91 26.733305157578258 33.0 25.0 35.0 2.0 35.0 92-93 26.293197819272027 33.0 24.5 35.0 2.0 35.0 94-95 25.78571583556051 33.0 22.5 35.0 2.0 35.0 96-97 25.190208516310648 32.5 19.0 35.0 2.0 35.0 98-99 24.484528963324035 32.0 4.5 34.5 2.0 35.0 100-101 22.697461729242942 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18216086131803522 1101 2 0.13485539585646578 1101 3 0.09655417622657936 1101 4 0.06796382812181889 1101 5 0.14996221474675764 1101 6 0.19517315865155638 1101 7 0.12492980567179046 1101 8 0.1440301446690384 1101 9 0.3063567222797161 1101 10-11 0.38626014856933466 1101 12-13 0.2284241730654415 1101 14-15 0.2725425296043369 1101 16-17 0.4764457584116073 1101 18-19 0.42117220553099344 1101 20-21 0.39804429973265343 1101 22-23 0.5525316496977339 1101 24-25 0.5245370062799068 1101 26-27 0.530390292098069 1101 28-29 0.47206928176643714 1101 30-31 0.4032005701551924 1101 32-33 0.4947022382814765 1101 34-35 0.4000506886337831 1101 36-37 0.5387305343907798 1101 38-39 0.5459989958777456 1101 40-41 0.5668183574722718 1101 42-43 0.7423378273440946 1101 44-45 0.7846089108317429 1101 46-47 0.7148831331238412 1101 48-49 0.7075545647292998 1101 50-51 0.647830181926949 1101 52-53 0.7387365829463022 1101 54-55 1.0238743614524424 1101 56-57 1.1329626855163468 1101 58-59 1.1573645364317429 1101 60-61 1.1389546760671294 1101 62-63 0.8256362161974735 1101 64-65 0.7177149975828883 1101 66-67 0.8866814423202349 1101 68-69 1.02934581425178 1101 70-71 0.7912063334904289 1101 72-73 0.8671721098747369 1101 74-75 0.9103987902432422 1101 76-77 0.7461698115674338 1101 78-79 0.7408727885945048 1101 80-81 0.975298900204784 1101 82-83 0.9672789540582727 1101 84-85 0.8761552991487562 1101 86-87 0.9224358444535667 1101 88-89 0.8913364463555205 1101 90-91 0.9547685634327934 1101 92-93 1.329683811152929 1101 94-95 1.3532208194564284 1101 96-97 1.32659253361793 1101 98-99 1.344828741836693 1101 100-101 1.9181666650506095 1102 1 0.2512072708436861 1102 2 0.26808582477532283 1102 3 0.3143159895568246 1102 4 0.11197973029792507 1102 5 0.1803255398639152 1102 6 0.14162503061697151 1102 7 0.11338045499499572 1102 8 0.09213360117718139 1102 9 0.21422256978132737 1102 10-11 0.3431780569901477 1102 12-13 0.3157671649069229 1102 14-15 0.5424000941355018 1102 16-17 0.564347627676554 1102 18-19 0.5314080377143426 1102 20-21 0.3628839433974136 1102 22-23 0.386105154211414 1102 24-25 0.3887548401054488 1102 26-27 0.48405630100096886 1102 28-29 0.5477275086534519 1102 30-31 0.4833724758411009 1102 32-33 0.656750860617322 1102 34-35 0.6531595421445004 1102 36-37 0.7792744301421379 1102 38-39 0.8580291063773657 1102 40-41 0.876905703595078 1102 42-43 0.9615899804702508 1102 44-45 0.8695484949298802 1102 46-47 1.111726414971045 1102 48-49 1.1723081068302221 1102 50-51 1.1106652246372377 1102 52-53 1.0699916071682338 1102 54-55 1.0275845561124228 1102 56-57 0.9554760527841353 1102 58-59 1.0464743323632177 1102 60-61 1.1100527293107731 1102 62-63 0.8807369119763848 1102 64-65 1.0458770188092714 1102 66-67 1.0189220946256832 1102 68-69 0.9326913296518278 1102 70-71 0.9526099768389642 1102 72-73 0.8527230933502814 1102 74-75 1.0578128863216314 1102 76-77 1.2082084148122085 1102 78-79 1.0852375586524552 1102 80-81 0.9347055351912914 1102 82-83 1.2408709782355913 1102 84-85 1.3336774839994234 1102 86-87 1.2878743636319072 1102 88-89 1.2046228569456972 1102 90-91 1.3497958850244842 1102 92-93 1.3464301739489137 1102 94-95 1.1091772865057479 1102 96-97 1.2246099276993085 1102 98-99 1.5701494897305324 1102 100-101 1.8293345075254557 1103 1 -0.02413346163150365 1103 2 0.026258754808928586 1103 3 0.18316802664552512 1103 4 -0.06726680592068135 1103 5 0.0038171510702298406 1103 6 0.043370537331988146 1103 7 0.06232537987444431 1103 8 0.07839969132184166 1103 9 0.05595216900554334 1103 10-11 0.15676318697327218 1103 12-13 0.1041319479141336 1103 14-15 0.18121868896274407 1103 16-17 0.04081807428185158 1103 18-19 0.2071426383333801 1103 20-21 0.06812336939935193 1103 22-23 -0.05599730796263458 1103 24-25 0.12853079023762604 1103 26-27 0.17893676235141953 1103 28-29 -0.004556743926940499 1103 30-31 -0.14476335885382952 1103 32-33 -0.19068874737428132 1103 34-35 0.0018113075770926912 1103 36-37 -2.0133667197796967E-4 1103 38-39 -0.02860511432164259 1103 40-41 -0.05429854991519534 1103 42-43 -0.077514135551489 1103 44-45 0.0767888109542767 1103 46-47 0.2351792537441426 1103 48-49 0.3797136415743054 1103 50-51 0.2851926492842196 1103 52-53 0.25548532055444184 1103 54-55 0.17662803105231006 1103 56-57 0.2957373640395957 1103 58-59 0.3284159263004369 1103 60-61 0.24261584452218443 1103 62-63 0.2518272891067923 1103 64-65 0.3430394910790966 1103 66-67 0.2955683870844048 1103 68-69 0.4533590291430549 1103 70-71 0.29830690094794576 1103 72-73 0.3041662249009143 1103 74-75 0.308462968017718 1103 76-77 0.31015459170084725 1103 78-79 0.26253301953795827 1103 80-81 0.16485106050379983 1103 82-83 0.3216749707168951 1103 84-85 0.24629795252317876 1103 86-87 0.2924818072748856 1103 88-89 0.46581331178702 1103 90-91 0.2914965087043946 1103 92-93 0.3092763030154515 1103 94-95 0.4251145000712846 1103 96-97 0.32997090154328035 1103 98-99 0.35109312939099624 1103 100-101 0.6397601408362021 1104 1 0.1297160546291316 1104 2 0.15807287455870522 1104 3 0.14912414041169342 1104 4 0.1620334233212688 1104 5 0.15700613968584065 1104 6 0.17343620155081396 1104 7 0.24205203799310482 1104 8 0.34809410014960207 1104 9 0.28375892581480855 1104 10-11 0.2208877935707818 1104 12-13 0.38350842924452166 1104 14-15 0.3024303686456946 1104 16-17 0.27612011997482 1104 18-19 0.4517564239338725 1104 20-21 0.3113517468326208 1104 22-23 0.49576547519003356 1104 24-25 0.42597585959303075 1104 26-27 0.32468631552389127 1104 28-29 0.31385036451615633 1104 30-31 0.3475070106531035 1104 32-33 0.16482447289224922 1104 34-35 0.029355553215665964 1104 36-37 0.22698496126484713 1104 38-39 0.36650940951563626 1104 40-41 0.40155780474929514 1104 42-43 0.4503652307305188 1104 44-45 0.09239256236275395 1104 46-47 0.060903162902164354 1104 48-49 0.19274667767020048 1104 50-51 0.2677760894676098 1104 52-53 0.16244927322958347 1104 54-55 0.059737707782311134 1104 56-57 0.030313315005130903 1104 58-59 -0.07434930468997436 1104 60-61 0.16228281231175146 1104 62-63 -0.008233615427535312 1104 64-65 0.1168804107667043 1104 66-67 0.15339588251899627 1104 68-69 0.07157769293447913 1104 70-71 0.23491446010402584 1104 72-73 0.1631314306358469 1104 74-75 0.15924928732455257 1104 76-77 0.177161478672911 1104 78-79 0.12573094470406332 1104 80-81 0.28699524980324753 1104 82-83 0.047209949541151985 1104 84-85 0.09293533255970488 1104 86-87 -0.017107742789221447 1104 88-89 -0.11867958754079311 1104 90-91 0.17988797818413005 1104 92-93 0.31154322417953395 1104 94-95 0.48996034457530513 1104 96-97 0.4910510099475225 1104 98-99 0.6899013141056045 1104 100-101 0.9751636940863868 1105 1 0.27142280435314703 1105 2 0.23336659794618342 1105 3 0.22554068222949297 1105 4 0.19177784454820568 1105 5 0.21988972140270846 1105 6 0.1941857547232928 1105 7 0.2245296637619134 1105 8 0.2510339624215874 1105 9 0.28232152109420383 1105 10-11 0.2058693783628769 1105 12-13 0.1407767688860062 1105 14-15 0.45651440507527496 1105 16-17 0.45047813395789404 1105 18-19 0.32941192648341655 1105 20-21 0.3805767474897195 1105 22-23 0.34515092306891404 1105 24-25 0.4406045327781172 1105 26-27 0.5053767133318061 1105 28-29 0.5255743302418523 1105 30-31 0.43641870636356117 1105 32-33 0.3786979747058439 1105 34-35 0.6382603165284948 1105 36-37 0.47311095019929184 1105 38-39 0.7465707168804059 1105 40-41 0.7812926158989555 1105 42-43 0.9924520302626689 1105 44-45 1.0410431268110543 1105 46-47 0.9641461122266648 1105 48-49 0.8410773990338143 1105 50-51 0.7913400810830282 1105 52-53 0.8729925136490664 1105 54-55 0.6938648170710664 1105 56-57 0.7225542864607419 1105 58-59 0.8737657238281429 1105 60-61 0.7910093867216759 1105 62-63 0.8372519481008212 1105 64-65 0.9365230801669071 1105 66-67 0.7871098406223389 1105 68-69 0.8472875244542273 1105 70-71 0.9184648826188742 1105 72-73 0.8641286609622938 1105 74-75 0.8865280255624768 1105 76-77 0.9487948304050278 1105 78-79 1.1532379428116144 1105 80-81 1.1136335626350835 1105 82-83 1.2506897831636117 1105 84-85 1.169784738541935 1105 86-87 1.043547450923647 1105 88-89 1.043984933696656 1105 90-91 1.012344494637901 1105 92-93 1.0743751414638716 1105 94-95 1.2587542059536396 1105 96-97 1.1862222335978387 1105 98-99 1.183414101258002 1105 100-101 1.0091188897705585 1106 1 0.02782389703276067 1106 2 -0.10062607034345916 1106 3 -0.0503140352590421 1106 4 -0.07035645455160733 1106 5 -0.0733211926549231 1106 6 -0.04423460138835367 1106 7 -0.0035981670142888333 1106 8 0.02923557030246826 1106 9 -0.012993898143747629 1106 10-11 -0.5324638429189008 1106 12-13 -0.521979593283497 1106 14-15 -0.5100762434555506 1106 16-17 -0.26875486723103137 1106 18-19 -0.19625702131707357 1106 20-21 -0.26641086432593397 1106 22-23 -0.10107052147618134 1106 24-25 -0.15729963129366809 1106 26-27 -0.15270223347790335 1106 28-29 0.05243131942829393 1106 30-31 -0.11494528545549798 1106 32-33 -0.1071631827985371 1106 34-35 -0.14342004991548407 1106 36-37 -0.07186610282114714 1106 38-39 -0.36586240603189424 1106 40-41 -0.33512703605711636 1106 42-43 -0.3760579729927258 1106 44-45 -0.04736511003999766 1106 46-47 0.08172859396815824 1106 48-49 -0.03934044122548386 1106 50-51 -0.18790369474780988 1106 52-53 -0.28048022951460894 1106 54-55 -0.3763384326600132 1106 56-57 -0.38725152229202564 1106 58-59 -0.18360659047294092 1106 60-61 -0.1766640337052081 1106 62-63 -0.34278099505718984 1106 64-65 -0.19403585659355826 1106 66-67 0.0880558506432898 1106 68-69 -0.24321681371030124 1106 70-71 -0.12303739261749413 1106 72-73 0.036483565989751554 1106 74-75 -0.2081839960363432 1106 76-77 -0.1553087335422063 1106 78-79 -0.12018761485129659 1106 80-81 -0.19072530194058146 1106 82-83 -0.23634791569841695 1106 84-85 -0.25307130233446884 1106 86-87 -0.09648220145703235 1106 88-89 -0.14804032744244822 1106 90-91 -0.2768161979115007 1106 92-93 -0.17034413399895598 1106 94-95 -0.06568193881225781 1106 96-97 0.09585722269935104 1106 98-99 0.0666392753507381 1106 100-101 0.16827618538851752 1107 1 0.14999825317840987 1107 2 0.1193238513087671 1107 3 0.20234265792654327 1107 4 0.21275339006572835 1107 5 0.17747952253415633 1107 6 0.049834675233597636 1107 7 0.14510581252367416 1107 8 0.3111779905922276 1107 9 0.34690148703543855 1107 10-11 0.28880689279455396 1107 12-13 0.35565998051216496 1107 14-15 0.4637473829082168 1107 16-17 0.5590873529153484 1107 18-19 0.3228482178301846 1107 20-21 0.25983634816513046 1107 22-23 0.3979825991642727 1107 24-25 0.33615344174307893 1107 26-27 0.3709244136435288 1107 28-29 0.3097846286890018 1107 30-31 0.47055910135865275 1107 32-33 0.6086478606955694 1107 34-35 0.5692060786655375 1107 36-37 0.4485180137852751 1107 38-39 0.6176940067262748 1107 40-41 0.6569361786478964 1107 42-43 0.6361433113629431 1107 44-45 0.7042568636060622 1107 46-47 0.708275124233829 1107 48-49 0.6893873590580597 1107 50-51 0.6791459425100257 1107 52-53 0.5567037465475266 1107 54-55 0.643786885996775 1107 56-57 0.661017601129096 1107 58-59 0.6364967209129446 1107 60-61 0.6489538463995572 1107 62-63 0.7698641183369261 1107 64-65 0.8005874743249564 1107 66-67 0.8922473384575902 1107 68-69 0.7942574127530158 1107 70-71 0.902466011897868 1107 72-73 0.9933729279772443 1107 74-75 1.0322090746013295 1107 76-77 0.9238045717893151 1107 78-79 0.9381580995396668 1107 80-81 1.0354598557136505 1107 82-83 1.0459628666364544 1107 84-85 1.213886149960281 1107 86-87 1.3488514542717986 1107 88-89 1.4086685371429404 1107 90-91 1.3875478911338845 1107 92-93 1.3529061226738257 1107 94-95 1.3547636659201565 1107 96-97 1.3645711952384296 1107 98-99 1.5819900551920796 1107 100-101 2.04329897188682 1108 1 0.17875473712625833 1108 2 0.17006827861845508 1108 3 0.06730363741156609 1108 4 0.2766255336238359 1108 5 0.16409665417582175 1108 6 0.20436432615186106 1108 7 0.2537435993361399 1108 8 0.2595073517773301 1108 9 0.2274790841194161 1108 10-11 0.24664931533767032 1108 12-13 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-2.6459703139725086 2316 74-75 -2.590940009065168 2316 76-77 -2.9574370589024177 2316 78-79 -2.895611122676648 2316 80-81 -3.1334551435370415 2316 82-83 -3.0054271032461237 2316 84-85 -2.812041181481245 2316 86-87 -3.16036989187554 2316 88-89 -3.2879727416148476 2316 90-91 -3.4693931357590913 2316 92-93 -3.3458314109846903 2316 94-95 -3.8178042191405375 2316 96-97 -3.4480753431914977 2316 98-99 -3.475950937861093 2316 100-101 -4.012041190640918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4061.0 3 710.0 4 689.0 5 638.0 6 741.0 7 987.0 8 1235.0 9 1547.0 10 2102.0 11 2961.0 12 4644.0 13 6615.0 14 7769.0 15 10508.0 16 14563.0 17 20002.0 18 15387.0 19 8449.0 20 7814.0 21 8045.0 22 8471.0 23 9081.0 24 10173.0 25 10889.0 26 12490.0 27 14925.0 28 17128.0 29 20190.0 30 24190.0 31 30132.0 32 38318.0 33 49727.0 34 68658.0 35 99046.0 36 139873.0 37 196820.0 38 167376.0 39 23057.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.2044314709806 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19.832197713634763 26.64402517506978 33.653764797916416 19.870012313379043 20-21 21.280417138525003 25.40406513441109 33.612604147563886 19.702913579500017 22-23 20.762919957403383 25.88718135845015 33.66905189441976 19.68084678972671 24-25 20.3249427441773 25.960541451514267 34.01397580841063 19.70053999589781 26-27 20.05045614224133 26.254633377711674 33.72536564870596 19.969544831341036 28-29 19.708509495753194 26.56040826822572 33.98820688716277 19.742875348858316 30-31 19.87987093571606 26.27309151508705 33.91795199092295 19.929085558273943 32-33 19.601157244359143 26.522877794428396 33.51801339718347 20.357951564028987 34-35 20.165538852272153 26.299374087831946 33.57492342161537 19.96016363828054 36-37 20.417644047825547 26.15134043207849 33.266375929742935 20.16463959035303 38-39 20.11170174301793 26.357480552392865 33.26456544127482 20.266252263314385 40-41 19.941954354628553 26.651656654478124 33.182949646985875 20.22343934390745 42-43 19.996833161993216 26.928994292601654 32.56251550923464 20.51165703617049 44-45 19.92772701704962 26.9936991572091 32.27265207341687 20.805921752324412 46-47 20.489482391869533 27.43781611912077 31.40373434176318 20.668967147246516 48-49 20.53412086953603 27.70765602241585 30.712304559802465 21.045918548245655 50-51 20.6029535448069 28.052293353744123 30.39252163249136 20.952231468957617 52-53 20.739066390188658 28.26331929663616 30.0082990691329 20.989315244042288 54-55 20.30050342336397 28.202297811628235 30.042258441642556 21.454940323365246 56-57 20.73309395288698 28.450325091752525 29.601827583697304 21.21475337166319 58-59 21.015374232989668 27.78652065587654 29.76765683305545 21.430448278078345 60-61 20.632779073141364 27.82830063314768 30.517217099051365 21.021703194659594 62-63 20.802718072735573 27.9214957528932 30.56106802223723 20.714718152133997 64-65 20.64516342451434 28.16098037696046 30.39770632923835 20.796149869286854 66-67 20.56682691194449 28.13081987835347 30.197941431792263 21.104411777909778 68-69 20.484740745781167 28.21605841347608 30.290980538922156 21.0082203018206 70-71 20.5062429701604 28.357545912532256 29.9798674845698 21.15634363273755 72-73 20.32990117520736 28.388580729283586 29.90278203771609 21.378736057792963 74-75 20.17434284685308 28.848523346146266 29.748595232795054 21.228538574205597 76-77 20.165300896407846 28.42552479513446 29.98618002455834 21.42299428389935 78-79 20.16799295586593 28.40689799570187 29.921831096751443 21.503277951680758 80-81 20.14945477506636 28.60313523242009 29.819351704111497 21.42805828840206 82-83 20.039384832916912 28.262516404439797 30.005911978159233 21.692186784484058 84-85 20.101984496768743 28.22917828337911 29.73801202530569 21.93082519454646 86-87 20.420967472886193 28.125158009293187 29.47599274728484 21.977881770535774 88-89 20.33313752945919 27.91131454970859 29.346337987101517 22.409209933730708 90-91 20.01699483491238 27.607107394974424 29.85315725373676 22.522740516376434 92-93 20.0698451403755 27.584152052587058 29.83129421438394 22.514708592653502 94-95 19.854139947394735 27.391030263621747 29.89809738306292 22.856732405920596 96-97 19.819708704234284 27.322741361475256 29.770652194972808 23.086897739317653 98-99 19.769116359173125 27.639870294550096 29.54178208406845 23.04923126220833 100-101 19.674488408203615 27.247738675895622 30.4005504106816 22.677222505219166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2383.0 1 1530.5 2 856.5 3 1060.0 4 889.5 5 887.0 6 1652.0 7 3470.0 8 5608.0 9 6292.0 10 5211.0 11 4124.0 12 3916.5 13 3988.0 14 4053.5 15 3975.5 16 3928.5 17 3970.5 18 4098.5 19 4345.0 20 4574.0 21 4876.5 22 5344.0 23 6111.0 24 6986.0 25 7934.0 26 9072.0 27 10498.5 28 11640.0 29 12806.0 30 14731.0 31 16772.5 32 18815.0 33 20207.5 34 21386.0 35 22713.0 36 24683.0 37 27347.5 38 28840.0 39 29477.5 40 30911.5 41 32706.5 42 33370.0 43 34664.0 44 37527.0 45 39926.5 46 39734.5 47 39943.5 48 44329.0 49 42972.0 50 36362.5 51 31801.5 52 28447.5 53 27382.5 54 26621.0 55 24511.5 56 21850.0 57 19711.5 58 18577.5 59 17133.5 60 14457.0 61 11980.5 62 9819.0 63 7052.5 64 4702.5 65 3603.0 66 2702.0 67 1978.0 68 1458.5 69 988.5 70 695.5 71 516.0 72 411.0 73 363.5 74 273.0 75 195.0 76 158.0 77 98.0 78 55.5 79 41.5 80 25.0 81 18.0 82 16.0 83 9.0 84 6.0 85 6.5 86 5.5 87 3.0 88 3.5 89 3.5 90 2.0 91 1.0 92 1.0 93 2.5 94 2.5 95 1.0 96 1.5 97 2.5 98 3.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36225970813565495 2 0.30876979810624966 3 0.3019774285787061 4 0.31027921355681487 5 0.31027921355681487 6 0.2245255482715778 7 0.20999742455988746 8 0.21584640943082775 9 0.20980874762856683 10-11 0.2305632100738387 12-13 0.2195256095915805 14-15 0.2132049323923386 16-17 0.2154690555681864 18-19 0.2094785629987557 20-21 0.20178997804743906 22-23 0.20589370130366325 24-25 0.19263914687838735 26-27 0.1947617623557447 28-29 0.21480868630856415 30-31 0.22645948681761452 32-33 0.2196199480572408 34-35 0.22985567158138628 36-37 0.20061074722668493 38-39 0.1987239779134784 40-41 0.19362970076782077 42-43 0.20523333204404098 44-45 0.20924271683460485 46-47 0.21348794778931957 48-49 0.22344065591648402 50-51 0.22664816374893515 52-53 0.2514591802176011 54-55 0.22211991739723946 56-57 0.18905428518329492 58-59 0.18952597751159656 60-61 0.1854694234882025 62-63 0.1941013930961224 64-65 0.18594111581650413 66-67 0.18372416187348647 68-69 0.17933742322028126 70-71 0.19084671603084113 72-73 0.19145991605763324 74-75 0.19787493172253545 76-77 0.20037490106253414 78-79 0.19919567024178006 80-81 0.19820511635234664 82-83 0.18787505436254084 84-85 0.18028080787688452 86-87 0.1817430540946196 88-89 0.18693166970593758 90-91 0.19287499304253816 92-93 0.1847147157629199 94-95 0.18339397724367532 96-97 0.18372416187348647 98-99 0.19598816240932893 100-101 0.17867705396065897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1060013.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.519621527782206 #Duplication Level Percentage of deduplicated Percentage of total 1 71.91753309956142 33.455764210033465 2 14.02254720104034 13.046471772957164 3 5.4868032651650935 7.657320338786394 4 2.715580191031558 5.053110508605223 5 1.541272032377194 3.5849695808771376 6 0.9623678698825695 2.686139344646105 7 0.634885598815758 2.0674246439243906 8 0.4711164011529375 1.7532925341732373 9 0.3302251190513773 1.3825752801513203 >10 1.7332798478769256 14.419923245150802 >50 0.11149348779826318 3.57763429952307 >100 0.06721041346700672 5.9703002526995235 >500 0.0034518941876153816 1.237227920351472 >1k 0.0016244207941719445 1.1958547466521328 >5k 4.061051985429861E-4 1.613648233574912 >10k+ 2.0305259927149306E-4 1.2983430878936466 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13745 1.2966822105011921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9943 0.9380073640606295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7140 0.6735766448147334 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2194 0.20697859365875704 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2158 0.20358240889498525 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.18773354666405034 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1516 0.14301711394105543 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1365 0.12877200562634608 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1287 0.12141360530484059 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1079 0.10179120444749262 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1071 0.10103649672221 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.43384656603268E-5 0.0 10-11 0.0 0.0 0.0 9.43384656603268E-5 0.0 12-13 0.0 0.0 0.0 9.43384656603268E-5 0.0 14-15 0.0 0.0 0.0 9.43384656603268E-5 0.0 16-17 0.0 0.0 0.0 9.43384656603268E-5 0.0 18-19 0.0 0.0 0.0 9.43384656603268E-5 0.0 20-21 0.0 0.0 0.0 1.886769313206536E-4 0.0 22-23 0.0 0.0 0.0 7.547077252826144E-4 0.0 24-25 0.0 0.0 0.0 0.0010377231222635948 0.0 26-27 0.0 0.0 0.0 0.0015565846833953923 0.0 28-29 0.0 0.0 0.0 0.0033490155309416018 0.0 30-31 0.0 0.0 0.0 0.007877261882637288 0.0 32-33 0.0 0.0 0.0 0.013396062123766407 0.0 34-35 0.0 0.0 0.0 0.0202356008841401 0.0 36-37 0.0 0.0 0.0 0.026650616549042324 0.0 38-39 0.0 0.0 0.0 0.032310924488661935 0.0 40-41 0.0 0.0 0.0 0.037782555496960885 0.0 42-43 0.0 0.0 0.0 0.044480586558844085 0.0 44-45 0.0 0.0 0.0 0.05179181764751942 0.0 46-47 0.0 0.0 0.0 0.06584824903090811 0.0 48-49 0.0 0.0 0.0 0.0828763420825971 0.0 50-51 0.0 0.0 0.0 0.09764031195843825 0.0 52-53 0.0 0.0 0.0 0.1238664054120091 0.0 54-55 0.0 0.0 0.0 0.1743846537731141 0.0 56-57 0.0 0.0 0.0 0.2634873345892928 0.0 58-59 0.0 0.0 0.0 0.3599012464941468 0.0 60-61 0.0 0.0 0.0 0.4618339586401299 0.0 62-63 0.0 0.0 0.0 0.5962191029732655 0.0 64-65 0.0 0.0 0.0 0.7537643406260113 0.0 66-67 0.0 0.0 0.0 0.9947991203881461 0.0 68-69 0.0 0.0 0.0 1.2824371021864827 0.0 70-71 0.0 0.0 0.0 1.5341321285682346 0.0 72-73 0.0 0.0 0.0 1.8239870643095886 0.0 74-75 0.0 0.0 0.0 2.1434642782682856 0.0 76-77 0.0 0.0 0.0 2.4953939244141345 0.0 78-79 0.0 0.0 0.0 2.824493661870185 0.0 80-81 0.0 0.0 0.0 3.1344898600300186 0.0 82-83 0.0 0.0 0.0 3.4329767653792924 0.0 84-85 0.0 0.0 0.0 3.8985842626458354 0.0 86-87 0.0 0.0 0.0 4.42518157796178 0.0 88-89 0.0 0.0 0.0 5.119276839057634 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8045 0.0 34.637768 1 GTATCAA 12240 0.0 33.450306 1 TATCAAC 15310 0.0 26.761156 2 ATCAACG 15210 0.0 26.748339 3 TCAACGC 15350 0.0 26.444954 4 GTACATG 8790 0.0 26.346228 1 CAACGCA 15540 0.0 26.121626 5 TACATGG 8805 0.0 26.01899 2 AACGCAG 15715 0.0 25.877762 6 GAGTACT 9235 0.0 24.203016 12-13 ACATGGG 9030 0.0 24.106268 3 CTAAGGG 300 0.0 23.765272 2 ACGCAGA 17405 0.0 23.228592 7 CGCAGAG 17440 0.0 23.100252 8 AGTACTT 9450 0.0 22.87317 12-13 CATGGGG 2475 0.0 22.470049 4 GTACTTT 9890 0.0 22.455984 14-15 CATGGGA 6385 0.0 22.110006 4 GCAGAGT 17935 0.0 22.091846 9 GTCGGGA 365 0.0 20.835308 2 >>END_MODULE