FastQCFastQC Report
Thu 26 May 2016
SRR937458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060013
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141661.3363987045441896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113481.0705529083133885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82310.7764999108501499No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29870.2817889969273962No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.24056308743383337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23850.22499724059987944No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18400.17358277681500134No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15290.1442435139946397No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA13620.1284889902293651No Hit
TATTAGGGCATTGTCACCACAATATAGATCTTCCTTGTCCAAGAATGCAA11930.11254578953276988No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.10990431249428073No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11440.10792320471541388No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.10641378926484864No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10820.1020742198444736No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG405.3884106E-447.4992459
GTATCAA129850.033.053651
GGTATCA85150.032.0406151
TAAGGTG4150.028.6207525
TAATCGA350.00839572427.14114630-31
ATCAACG163900.026.0887173
TATCAAC164700.025.990842
TCAACGC166000.025.7300574
CAACGCA168550.025.3689735
GTACATG93000.024.9919151
AACGCAG172050.024.9010756
TTAGGGC6300.024.8864273
TACATGG93650.024.5035212
GTAAGGT3750.024.0719624
GAGTACT101100.024.00802612-13
AGTACTT103200.023.84167712-13
TATTAGG7400.023.1230131
ATTAGGG7200.023.0953582
ACATGGG94050.023.035373
GGACCGT1050.00219691522.617626
Produced by FastQC (version 0.11.5)