##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937458_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1060013 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13938979993642 33.0 31.0 34.0 31.0 34.0 2 32.32984689810408 34.0 31.0 34.0 31.0 34.0 3 32.38637922365103 34.0 31.0 34.0 30.0 34.0 4 36.041502321197946 37.0 35.0 37.0 35.0 37.0 5 35.9252452564261 37.0 35.0 37.0 35.0 37.0 6 36.033268459915114 37.0 35.0 37.0 35.0 37.0 7 35.98864447888847 37.0 35.0 37.0 35.0 37.0 8 35.9729484449719 37.0 35.0 37.0 35.0 37.0 9 37.63802896756926 39.0 37.0 39.0 35.0 39.0 10-11 37.56883123131509 39.0 37.0 39.0 35.0 39.0 12-13 37.4824667244647 39.0 37.0 39.0 35.0 39.0 14-15 38.57811036279744 40.0 38.0 41.0 34.0 41.0 16-17 38.586410261006236 40.0 38.0 41.0 34.5 41.0 18-19 38.69928717855347 40.0 38.0 41.0 34.5 41.0 20-21 38.66263008095184 40.0 38.0 41.0 34.0 41.0 22-23 38.54107072271755 40.0 38.0 41.0 34.0 41.0 24-25 38.380301468000866 40.0 38.0 41.0 34.0 41.0 26-27 38.13616153764152 40.0 37.5 41.0 33.5 41.0 28-29 38.059229462280186 40.0 37.0 41.0 33.0 41.0 30-31 38.007223024623286 40.0 37.0 41.0 34.0 41.0 32-33 37.82352999444346 40.0 36.5 41.0 33.0 41.0 34-35 37.63428939079049 40.0 36.0 41.0 33.0 41.0 36-37 37.41677413390213 40.0 35.5 41.0 33.0 41.0 38-39 37.21200777726311 40.0 35.0 41.0 33.0 41.0 40-41 36.9652235397113 40.0 35.0 41.0 32.0 41.0 42-43 36.63535022683684 39.0 35.0 41.0 31.0 41.0 44-45 36.14962646684522 39.0 35.0 41.0 30.5 41.0 46-47 35.535050041839106 39.0 35.0 41.0 28.0 41.0 48-49 34.98659591910665 39.0 35.0 41.0 24.5 41.0 50-51 34.680093074330216 39.0 35.0 40.5 23.0 41.0 52-53 34.40982091729064 38.5 34.5 40.0 22.5 41.0 54-55 34.180022792173304 38.0 34.0 40.0 20.5 41.0 56-57 33.92685702911191 38.0 34.0 40.0 19.5 41.0 58-59 33.65060428504179 38.0 34.0 40.0 18.0 41.0 60-61 33.30877451502953 37.0 33.0 40.0 14.0 41.0 62-63 32.93751680403919 37.0 33.0 40.0 10.0 41.0 64-65 32.580827310608456 36.0 33.0 39.5 7.5 41.0 66-67 32.18466754653009 36.0 32.0 39.0 4.0 40.5 68-69 31.74909552996048 35.0 32.0 39.0 2.0 40.0 70-71 31.3486188376935 35.0 31.0 38.0 2.0 40.0 72-73 30.93450221836902 35.0 31.0 37.5 2.0 39.5 74-75 30.50291883212753 35.0 31.0 37.0 2.0 39.0 76-77 29.20444513416345 33.5 29.0 35.5 2.0 38.0 78-79 29.759825587044688 34.0 30.0 36.0 2.0 38.0 80-81 29.56222282179558 34.0 30.0 35.5 2.0 37.0 82-83 29.219783153602833 34.0 30.0 35.0 2.0 37.0 84-85 28.889280603162415 34.0 29.5 35.0 2.0 36.5 86-87 28.526836463326394 34.0 29.0 35.0 2.0 36.0 88-89 28.143824651207108 34.0 29.0 35.0 2.0 36.0 90-91 27.70001830166234 34.0 27.5 35.0 2.0 35.0 92-93 27.295870428004186 34.0 27.0 35.0 2.0 35.0 94-95 26.905643138338867 33.0 26.0 35.0 2.0 35.0 96-97 26.490150592492732 33.0 25.0 35.0 2.0 35.0 98-99 25.901410643077018 33.0 24.5 35.0 2.0 35.0 100-101 24.46231225466103 31.5 17.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26666009578049454 1101 2 0.29355760344645887 1101 3 0.28583781015238685 1101 4 0.20998838906447048 1101 5 0.08062134715841296 1101 6 0.19098909480489823 1101 7 0.09415116290228553 1101 8 0.14404321951641208 1101 9 0.24590788067249747 1101 10-11 0.24829293668176433 1101 12-13 0.16253424871183597 1101 14-15 0.23184412672416954 1101 16-17 0.31346697110706145 1101 18-19 0.36948883837617785 1101 20-21 0.23583214796648377 1101 22-23 0.2168191401727384 1101 24-25 0.14712556488450446 1101 26-27 0.2891397960270794 1101 28-29 0.15694427676873346 1101 30-31 0.27031527880454576 1101 32-33 0.31050767996210027 1101 34-35 0.46476275576385717 1101 36-37 0.343977044970984 1101 38-39 0.25363679355267976 1101 40-41 0.3351355635913933 1101 42-43 0.41958494718956274 1101 44-45 0.41594586983585913 1101 46-47 0.31883979165668563 1101 48-49 0.1847353567664456 1101 50-51 0.277947462908827 1101 52-53 0.23836489634484082 1101 54-55 0.04615552100812437 1101 56-57 0.21459324846914996 1101 58-59 0.1565860275023354 1101 60-61 0.1871519383175979 1101 62-63 0.09111574503776154 1101 64-65 0.07899945575109513 1101 66-67 0.11221158617746596 1101 68-69 0.018506050073998637 1101 70-71 -0.2528756206443319 1101 72-73 -0.2929728928725659 1101 74-75 -0.31651400414713393 1101 76-77 -0.14795874999211733 1101 78-79 -0.02919109421464583 1101 80-81 -0.1196838235390878 1101 82-83 -0.12595933144071836 1101 84-85 -0.2487662839990996 1101 86-87 -0.27227866416576774 1101 88-89 -0.1868851976262249 1101 90-91 0.1916894302105483 1101 92-93 0.28505257164135855 1101 94-95 0.07135963711067816 1101 96-97 0.030221707850749624 1101 98-99 -0.04242892941598697 1101 100-101 0.258373505651047 1102 1 0.23769709518796134 1102 2 0.1886530235477295 1102 3 0.13727817190419955 1102 4 0.08515990729334533 1102 5 0.1220956877182715 1102 6 0.19226887714014396 1102 7 0.11572195286847631 1102 8 0.1788047690627721 1102 9 0.34771280427301576 1102 10-11 0.2143716601243213 1102 12-13 0.16119850036010064 1102 14-15 0.08607613599809127 1102 16-17 0.147359834148439 1102 18-19 0.1084766440465117 1102 20-21 0.25699982606561633 1102 22-23 0.23361246745808728 1102 24-25 0.21867361621043813 1102 26-27 0.29562480926714585 1102 28-29 0.25455722832251126 1102 30-31 0.17284791716780035 1102 32-33 0.20592480681553127 1102 34-35 0.2563006940697363 1102 36-37 0.10323707247651726 1102 38-39 0.18878705252270578 1102 40-41 0.2336175147467614 1102 42-43 0.5142868831440879 1102 44-45 0.46294519119898325 1102 46-47 0.12112790157863174 1102 48-49 0.02344325408015635 1102 50-51 0.17878546594742772 1102 52-53 0.11735736715444745 1102 54-55 0.14303426104480366 1102 56-57 0.14062809142381383 1102 58-59 0.06151268071106841 1102 60-61 -0.07071402659523329 1102 62-63 -0.10636367081792031 1102 64-65 -0.10603554453461328 1102 66-67 -0.1316026035168001 1102 68-69 -0.2500502067309611 1102 70-71 -0.26589574257980075 1102 72-73 -0.35593035635180925 1102 74-75 -0.2467568653490666 1102 76-77 -0.20136559318786595 1102 78-79 -0.06932264174935909 1102 80-81 -0.14540157791637043 1102 82-83 -0.1878604852799448 1102 84-85 -0.22576072888274723 1102 86-87 -0.34970862641414513 1102 88-89 -0.12083590526366805 1102 90-91 0.020999733201950477 1102 92-93 -0.1581927998125252 1102 94-95 -0.056680432991651486 1102 96-97 0.08906038561251606 1102 98-99 -0.17538735566725805 1102 100-101 -0.03813288425910244 1103 1 0.14026620476699492 1103 2 0.1529981596414629 1103 3 0.17214596371864843 1103 4 0.1226628961747096 1103 5 0.05378314371530024 1103 6 0.08839113567173484 1103 7 0.05034183357889788 1103 8 0.06038113830253877 1103 9 -0.06039154753824505 1103 10-11 0.12013347616863967 1103 12-13 0.15973602410510068 1103 14-15 0.14140365097571106 1103 16-17 0.02386174703250532 1103 18-19 0.07695930521364147 1103 20-21 0.0648209813678875 1103 22-23 0.13055376250717643 1103 24-25 -0.031418956087286176 1103 26-27 0.08928738448733498 1103 28-29 0.0659650412583872 1103 30-31 0.1494358434154961 1103 32-33 0.1409232791650652 1103 34-35 0.19588875979855658 1103 36-37 0.12163363725554177 1103 38-39 0.2602171658345185 1103 40-41 0.1734828822463328 1103 42-43 0.2322539622763813 1103 44-45 0.09890566822445379 1103 46-47 -0.12182082914308268 1103 48-49 -0.100154462414487 1103 50-51 -0.1291510933851896 1103 52-53 0.13489130756280332 1103 54-55 0.025968080569505503 1103 56-57 0.015412642892350448 1103 58-59 0.0010654610349263294 1103 60-61 0.027983817425010216 1103 62-63 0.15507461210294338 1103 64-65 0.14380919968356665 1103 66-67 0.2981877430605948 1103 68-69 0.411829683411046 1103 70-71 0.17541092993178609 1103 72-73 0.2878921240212051 1103 74-75 0.11958243578405359 1103 76-77 -0.2320323461999898 1103 78-79 0.2138456389132415 1103 80-81 0.08131379091158664 1103 82-83 0.06785533520119813 1103 84-85 -0.1754574404490583 1103 86-87 -0.09196161197452923 1103 88-89 -0.21480633526406478 1103 90-91 -0.0939356239361615 1103 92-93 0.06405045527853304 1103 94-95 -0.13557361701375825 1103 96-97 -0.03487191954562974 1103 98-99 0.0072046520639794664 1103 100-101 0.4441895429782434 1104 1 -0.033821684264360385 1104 2 0.09418934354012976 1104 3 0.09615857202575029 1104 4 0.0025512864299841453 1104 5 -0.04045860940278345 1104 6 -0.0023464211530708212 1104 7 -0.052768474537820964 1104 8 0.011917350759311773 1104 9 -0.132202073240542 1104 10-11 0.031509850648966164 1104 12-13 0.0736403738739142 1104 14-15 0.13555603858887366 1104 16-17 -0.007797440129941435 1104 18-19 -0.051643241703253295 1104 20-21 -0.18681212466622554 1104 22-23 -0.18779749249534916 1104 24-25 -0.011776758084408812 1104 26-27 -0.01483799920151796 1104 28-29 -0.2601716976777695 1104 30-31 -0.2011270621290535 1104 32-33 -0.2784243526490542 1104 34-35 -0.3179726071051192 1104 36-37 -0.3054606239248585 1104 38-39 -0.38002110979977743 1104 40-41 -0.2762733053435227 1104 42-43 -0.40835707436347235 1104 44-45 -0.3613958917864366 1104 46-47 -0.2940801250983185 1104 48-49 -0.5250995803381002 1104 50-51 -0.40301386876693357 1104 52-53 -0.4909804917922145 1104 54-55 -0.45525059293556325 1104 56-57 -0.48844731553326426 1104 58-59 -0.4369092932099292 1104 60-61 -0.773027372690386 1104 62-63 -0.600789999157108 1104 64-65 -0.4828348341976181 1104 66-67 -0.5841921963262742 1104 68-69 -0.6637072381415869 1104 70-71 -0.5058990518065656 1104 72-73 -0.41258530147516126 1104 74-75 -0.4929051793226016 1104 76-77 -0.5369654192077249 1104 78-79 -0.7489268287403306 1104 80-81 -0.6776264021956102 1104 82-83 -0.6907691645462499 1104 84-85 -0.7495130316212979 1104 86-87 -0.5713711196871607 1104 88-89 -0.42813733732335635 1104 90-91 -0.25966856678109096 1104 92-93 -0.38239823726527433 1104 94-95 -0.18820212849528062 1104 96-97 -0.22560899068834317 1104 98-99 -0.02983953529274075 1104 100-101 0.09035071172362663 1105 1 0.15502536930237198 1105 2 0.13853204710949285 1105 3 0.11384193558565414 1105 4 0.10755739745348336 1105 5 0.01648561600856624 1105 6 0.078866945560911 1105 7 0.20972216179714565 1105 8 0.05509288169554338 1105 9 0.08750647036456627 1105 10-11 0.17560022808680031 1105 12-13 0.12937184038813854 1105 14-15 0.1972367178981287 1105 16-17 0.14704349255679716 1105 18-19 0.12168969737049906 1105 20-21 0.15515426141904243 1105 22-23 0.13320455108348028 1105 24-25 0.08517577963309009 1105 26-27 0.12441366325970904 1105 28-29 0.17675867936581113 1105 30-31 0.18151171724328918 1105 32-33 0.25274925955721983 1105 34-35 0.27659794564680595 1105 36-37 0.3311125375112951 1105 38-39 0.4025471956725397 1105 40-41 0.3259298664748087 1105 42-43 0.362002736786188 1105 44-45 0.38271321298147853 1105 46-47 0.5584261337725209 1105 48-49 0.5210292012683837 1105 50-51 0.4631736082345981 1105 52-53 0.4812521345022702 1105 54-55 0.5375636155226147 1105 56-57 0.6093224894396698 1105 58-59 0.515924529662378 1105 60-61 0.6466299171692569 1105 62-63 0.6131015728872669 1105 64-65 0.6216238309708615 1105 66-67 0.582448186368353 1105 68-69 0.5971184728398562 1105 70-71 0.6586936451918071 1105 72-73 0.7088080447373173 1105 74-75 0.7665480154941982 1105 76-77 0.897656485854565 1105 78-79 0.8004558264665249 1105 80-81 0.8830641106046819 1105 82-83 0.7650926540417764 1105 84-85 0.7523851639840231 1105 86-87 0.8329371518788378 1105 88-89 0.7985808130735279 1105 90-91 0.8270069378711717 1105 92-93 0.9278811777849292 1105 94-95 0.9062928578370553 1105 96-97 0.9773496810511304 1105 98-99 0.6453320497621924 1105 100-101 0.7739463098154111 1106 1 -0.22885265255121823 1106 2 -0.07943948943582768 1106 3 -0.10331486818493829 1106 4 -0.03573826296660343 1106 5 -0.07167890870411497 1106 6 -0.09096104940030614 1106 7 -0.02795600017986999 1106 8 -0.010840319063099457 1106 9 -0.14873684192666303 1106 10-11 -0.1415900142276385 1106 12-13 -0.20842777958015546 1106 14-15 -0.1452292597104332 1106 16-17 -0.026492376916102955 1106 18-19 -0.03668161554350036 1106 20-21 -0.3029984624953599 1106 22-23 -0.1556009675147223 1106 24-25 -0.05138528728646463 1106 26-27 -0.026621516211498886 1106 28-29 -0.12241765322208664 1106 30-31 -0.2310137597425026 1106 32-33 -0.025502771524699597 1106 34-35 -0.19242009347451727 1106 36-37 -0.4027679612714721 1106 38-39 -0.6162518121403977 1106 40-41 -0.4140682050589106 1106 42-43 -0.8370534647367691 1106 44-45 -0.6620802818948093 1106 46-47 -0.6932497741989465 1106 48-49 -1.0946438334652484 1106 50-51 -0.748213719795757 1106 52-53 -0.5779553189314655 1106 54-55 -0.7318798361843406 1106 56-57 -0.9109104834414978 1106 58-59 -1.2032275290196068 1106 60-61 -1.019558761141333 1106 62-63 -0.6530541976500217 1106 64-65 -0.7290522822318763 1106 66-67 -0.5560050775166019 1106 68-69 -0.4265291238311484 1106 70-71 -0.6569547087118366 1106 72-73 -0.5947369716811828 1106 74-75 -0.5881951269504597 1106 76-77 -0.6306972762470018 1106 78-79 -0.4985537557109083 1106 80-81 -0.4833949068328991 1106 82-83 -0.662587206400957 1106 84-85 -0.4905723956038166 1106 86-87 -0.4172840025116962 1106 88-89 -0.5592637985439772 1106 90-91 -0.3783702984420252 1106 92-93 -0.36168290037280215 1106 94-95 -0.4446570445011666 1106 96-97 -0.42847083712245393 1106 98-99 -0.288790922694357 1106 100-101 -0.2820557318819219 1107 1 0.01421907131703648 1107 2 0.03677968031390577 1107 3 0.11473940571207919 1107 4 0.11052130382073955 1107 5 0.038984605790936655 1107 6 0.08613419863485916 1107 7 0.035774411696124275 1107 8 -0.024693565513366877 1107 9 0.0449573665719214 1107 10-11 0.06677633788302018 1107 12-13 0.05957166504322231 1107 14-15 0.03026172439238195 1107 16-17 0.15642724176306189 1107 18-19 0.2051589290278315 1107 20-21 -8.401658952550406E-6 1107 22-23 0.07723218053564551 1107 24-25 0.11720599235606244 1107 26-27 0.1369737351294802 1107 28-29 0.08971354789320429 1107 30-31 0.2692455104661704 1107 32-33 0.217877809750604 1107 34-35 0.1444281064157309 1107 36-37 0.1300124826709137 1107 38-39 0.21548465598541355 1107 40-41 0.26056562890047985 1107 42-43 0.40050510718970145 1107 44-45 0.4055188759728807 1107 46-47 0.28769457385218544 1107 48-49 0.26923741794562517 1107 50-51 0.27048632110188464 1107 52-53 0.028564594815151167 1107 54-55 0.09833406882169982 1107 56-57 0.26776069298409055 1107 58-59 0.30646086502576253 1107 60-61 0.2244626756406518 1107 62-63 0.26672410506990474 1107 64-65 0.1340445469265248 1107 66-67 -0.23092132237724172 1107 68-69 -0.19090539715956467 1107 70-71 -0.4475659157964742 1107 72-73 -0.3201608477755258 1107 74-75 -0.08000804436841236 1107 76-77 -0.095217871935656 1107 78-79 -0.125598046996501 1107 80-81 -0.16130338492149932 1107 82-83 -0.12504166882377987 1107 84-85 -0.13857176466574828 1107 86-87 -0.03494261779153973 1107 88-89 -0.10924674999291284 1107 90-91 0.21281536452936933 1107 92-93 0.3737637291617091 1107 94-95 0.14710619126659807 1107 96-97 0.33342749306406105 1107 98-99 0.13435269788896065 1107 100-101 0.1846462282764172 1108 1 0.060928730646914175 1108 2 0.035384288005850806 1108 3 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-0.6976552729545276 2316 64-65 -0.6583923780989736 2316 66-67 -0.7436529437388586 2316 68-69 -0.5974446972557637 2316 70-71 -0.5138605057789292 2316 72-73 -0.6324200413246928 2316 74-75 -0.5315585115993962 2316 76-77 -0.5881266204914191 2316 78-79 -0.6713367698823589 2316 80-81 -0.6290564703863346 2316 82-83 -0.7178968779779282 2316 84-85 -0.7076400098786273 2316 86-87 -0.7335381451365173 2316 88-89 -0.908075771974282 2316 90-91 -0.8447231765884062 2316 92-93 -0.9065803007720135 2316 94-95 -0.6367426753417327 2316 96-97 -0.5394321394356822 2316 98-99 -0.4067022337788124 2316 100-101 -0.6515304541660427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 7.0 5 26.0 6 63.0 7 147.0 8 373.0 9 740.0 10 1335.0 11 2307.0 12 3682.0 13 5320.0 14 6734.0 15 10028.0 16 14319.0 17 20756.0 18 17572.0 19 8859.0 20 6823.0 21 6872.0 22 6991.0 23 7434.0 24 8243.0 25 8959.0 26 10584.0 27 12769.0 28 15577.0 29 18942.0 30 23165.0 31 29071.0 32 36371.0 33 47060.0 34 65278.0 35 96308.0 36 141622.0 37 215031.0 38 190231.0 39 20408.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.65978824892926 20.093134229188728 15.765620369479002 19.48145715240301 2 15.685562346876877 22.46095095060155 39.34489482676155 22.508591875760015 3 19.23674520972856 27.703622502742892 31.182919454761404 21.876712832767144 4 12.468715006325395 18.097796913811433 37.29878784505473 32.13470023480844 5 12.296955081029264 39.82699667127103 32.71032887238363 15.165719375316078 6 29.31662158860316 37.559445025674215 17.300448202050354 15.823485183672275 7 26.680144488794006 36.30861130948394 20.129564448737895 16.881679752984162 8 27.80814952269453 33.19685701967806 19.529477468672553 19.465515988954852 9 28.169843200036226 16.266121264550527 20.375976521042666 35.18805901437057 10-11 24.64059403045057 28.109560920479275 26.08939701682904 21.160448032241113 12-13 26.830686904718664 26.089850422260284 27.671999777348095 19.407462895672957 14-15 23.409429884350473 26.15562261972259 28.103523258677015 22.33142423724992 16-17 20.077631123391885 29.971943740312618 28.38007647076026 21.57034866553523 18-19 19.99937736612664 26.71372898256908 33.61581414567557 19.671079505628704 20-21 21.31190843885877 25.462282066352017 33.8912352961709 19.334574198618316 22-23 20.924300142829917 25.904762443844447 33.92205863762521 19.248878775700422 24-25 20.62974023321511 26.046489249850357 34.129914995964654 19.19385552096988 26-27 20.25098278950899 26.452721857597172 33.91437490624938 19.38192044664446 28-29 19.82839379802544 26.616320042264753 34.19038948664368 19.364896673066127 30-31 19.9943962502046 26.3561145259289 34.09542129302282 19.55406793084369 32-33 19.83437938968673 26.547174421445774 33.74647292061512 19.871973268252372 34-35 20.340316581023064 26.342129766333056 33.71920910403929 19.598344548604594 36-37 20.671634856447177 26.170872835693608 33.34589610197258 19.811596205886637 38-39 20.350100635890527 26.48957391419605 33.39385165887664 19.76647379103678 40-41 20.233714114826892 26.78344510869206 33.180347788187504 19.80249298829354 42-43 20.264752765487508 27.086799676760283 32.594470487392854 20.05397707035936 44-45 20.279373932206493 27.208534234957494 32.20054848383935 20.311543348996665 46-47 20.828046448486955 27.695933917791578 31.334285522913397 20.141734110808073 48-49 20.92432872253865 27.980377589886913 30.504168582917657 20.591125104656786 50-51 20.958799561892167 28.365737023979893 30.139866209188 20.53559720493994 52-53 21.089572570876555 28.72656158609525 29.609806304079576 20.574059538948617 54-55 20.61974711630895 28.677289806823126 29.64317418748638 21.059788889381544 56-57 21.112193907055858 28.871579876850568 29.217519030426985 20.79870718566659 58-59 21.38407736508892 28.20196544759357 29.38515848390539 21.02879870341213 60-61 20.91144165213068 28.377623670653097 30.080857498917467 20.630077178298755 62-63 21.017572425998548 28.429132472903635 30.263638276134348 20.28965682496347 64-65 20.96318630054537 28.57733820245601 30.046565466649938 20.412910030348684 66-67 20.91082845210389 28.622856512137112 29.82439838001987 20.641916655739127 68-69 20.986063378468 28.71691196240046 29.70972997500974 20.587294684121797 70-71 20.844131156882035 28.902994585915454 29.50176082746155 20.751113429740954 72-73 20.695170719604384 28.9013436627664 29.39157349957029 21.01191211805893 74-75 20.622199916416122 29.49350621171627 29.01851203711653 20.865781834751083 76-77 20.588049391847083 28.947475172474302 29.495251473330985 20.969223962347634 78-79 20.51781440416297 28.967899450289757 29.318461188683536 21.19582495686374 80-81 20.552012097964838 29.250160139545457 29.225301953843964 20.97252580864574 82-83 20.33256321408592 29.09956424898279 29.30239271553355 21.265479821397744 84-85 20.306251538899126 29.02399126046805 29.071160582295846 21.59859661833698 86-87 20.623143301072723 28.789977104054383 28.839127444663415 21.74775215020948 88-89 20.499465572592037 28.723845839626495 28.594177618576378 22.182510969205094 90-91 20.322747576374113 28.528379926797086 28.843919483400093 22.30495301342871 92-93 20.194860788452694 28.541185627188998 28.871991414993104 22.391962169365204 94-95 20.09734786271489 28.413425118371187 28.787760150111367 22.70146686880255 96-97 20.187346758954845 28.479556382799082 28.572196756077524 22.760900102168556 98-99 20.101103868965915 28.87021591529803 28.258988923574456 22.769691292161596 100-101 19.705056524307054 28.916320249684404 28.752625252618646 22.625997973389893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2014.0 1 1388.5 2 919.0 3 1101.0 4 1002.5 5 1097.0 6 1900.5 7 3619.5 8 5426.0 9 6018.0 10 5279.0 11 4424.5 12 4212.5 13 4272.0 14 4292.0 15 4284.0 16 4298.0 17 4334.0 18 4390.5 19 4482.0 20 4745.0 21 5077.5 22 5511.5 23 6190.5 24 6949.5 25 7830.0 26 9029.0 27 10481.5 28 11515.0 29 12815.5 30 14642.0 31 16365.5 32 18326.0 33 19779.0 34 20971.5 35 22067.0 36 24106.5 37 26790.0 38 28334.5 39 29222.0 40 30407.0 41 32030.0 42 33156.0 43 34577.5 44 37447.5 45 39775.0 46 39137.0 47 39357.0 48 44155.0 49 42517.0 50 36158.0 51 32199.5 52 28553.0 53 27545.5 54 26956.0 55 24704.0 56 21938.0 57 19983.0 58 19222.0 59 18181.0 60 15202.0 61 12204.5 62 9962.0 63 7264.5 64 4860.0 65 3601.5 66 2740.5 67 2152.0 68 1586.5 69 996.0 70 700.5 71 516.0 72 397.5 73 367.0 74 311.5 75 246.0 76 169.0 77 95.0 78 57.0 79 35.5 80 22.5 81 14.0 82 7.5 83 4.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044056063463372624 2 0.0 3 0.0 4 0.0 5 0.014056431383388693 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005896154103770425 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010377231222635948 24-25 4.245230954714706E-4 26-27 0.0018867693132065362 28-29 0.0026414770384891504 30-31 8.018769581127779E-4 32-33 0.0 34-35 0.0 36-37 2.3584616415081702E-4 38-39 3.301846298111438E-4 40-41 0.0 42-43 0.011179108180748727 44-45 0.0 46-47 0.0 48-49 4.71692328301634E-5 50-51 0.0 52-53 9.43384656603268E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.773538626413072E-4 84-85 1.886769313206536E-4 86-87 0.0 88-89 0.0 90-91 0.007782923416976961 92-93 0.0024056308743383337 94-95 0.0 96-97 0.0 98-99 0.0013207385192445752 100-101 0.010848923550937584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1060013.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.388710863273545 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6236933197451 31.34894702866669 2 14.410111724241192 12.79292565669621 3 5.756095121656858 7.665169261701769 4 2.8042570943758474 4.979094293941324 5 1.6886933211462074 3.747945978455007 6 1.0576300274012362 2.816810009197773 7 0.7012111192895415 2.178810033977911 8 0.49249094413683775 1.7488830497656558 9 0.3805542874913586 1.5203082811709694 >10 1.8751023173218788 14.851693686721944 >50 0.1302069598235453 3.9917159221577028 >100 0.07247145947082995 6.1167876088102755 >500 0.004275602228321216 1.198134617923738 >1k 0.0025653613369927292 1.8405685925272313 >5k 2.1378011141606076E-4 0.7810744527269353 >10k+ 4.275602228321215E-4 2.4211315255588666 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14166 1.3363987045441896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11348 1.0705529083133885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8231 0.7764999108501499 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2987 0.2817889969273962 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2550 0.24056308743383337 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2385 0.22499724059987944 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1840 0.17358277681500134 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1529 0.1442435139946397 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 1362 0.1284889902293651 No Hit TATTAGGGCATTGTCACCACAATATAGATCTTCCTTGTCCAAGAATGCAA 1193 0.11254578953276988 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1165 0.10990431249428073 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1144 0.10792320471541388 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1128 0.10641378926484864 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1082 0.1020742198444736 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.43384656603268E-5 0.0 10-11 0.0 0.0 0.0 9.43384656603268E-5 0.0 12-13 0.0 0.0 0.0 9.43384656603268E-5 0.0 14-15 0.0 0.0 0.0 9.43384656603268E-5 0.0 16-17 0.0 0.0 0.0 9.43384656603268E-5 0.0 18-19 0.0 0.0 0.0 9.43384656603268E-5 0.0 20-21 0.0 0.0 0.0 2.830153969809804E-4 0.0 22-23 0.0 0.0 0.0 8.490461909429413E-4 0.0 24-25 0.0 0.0 0.0 0.0011320615879239217 0.0 26-27 0.0 0.0 0.0 0.001650923149055719 0.0 28-29 0.0 0.0 0.0 0.003490523229432092 0.0 30-31 0.0 0.0 0.0 0.008113108046788105 0.0 32-33 0.0 0.0 0.0 0.01372624675357755 0.0 34-35 0.0 0.0 0.0 0.020565785513951246 0.0 36-37 0.0 0.0 0.0 0.027075139644513795 0.0 38-39 0.0 0.0 0.0 0.03282978604979373 0.0 40-41 0.0 0.0 0.0 0.03830141705809269 0.0 42-43 0.0 0.0 0.0 0.045235294284126704 0.0 44-45 0.0 0.0 0.0 0.052405017674311535 0.0 46-47 0.0 0.0 0.0 0.06631994135920975 0.0 48-49 0.0 0.0 0.0 0.08334803441089873 0.0 50-51 0.0 0.0 0.0 0.09815917351957004 0.0 52-53 0.0 0.0 0.0 0.12396074387766942 0.0 54-55 0.0 0.0 0.0 0.17433748454028394 0.0 56-57 0.0 0.0 0.0 0.2645250577115564 0.0 58-59 0.0 0.0 0.0 0.36192952350584384 0.0 60-61 0.0 0.0 0.0 0.4646641126099397 0.0 62-63 0.0 0.0 0.0 0.5994737800385467 0.0 64-65 0.0 0.0 0.0 0.7582454177448767 0.0 66-67 0.0 0.0 0.0 1.001119797587388 0.0 68-69 0.0 0.0 0.0 1.2916351025883643 0.0 70-71 0.0 0.0 0.0 1.5450753905848325 0.0 72-73 0.0 0.0 0.0 1.8374302956661852 0.0 74-75 0.0 0.0 0.0 2.158464094308277 0.0 76-77 0.0 0.0 0.0 2.5137899252178983 0.0 78-79 0.0 0.0 0.0 2.8484556321479078 0.0 80-81 0.0 0.0 0.0 3.16368761515189 0.0 82-83 0.0 0.0 0.0 3.4659952283604065 0.0 84-85 0.0 0.0 0.0 3.9369800181695886 0.0 86-87 0.0 0.0 0.0 4.472350810791943 0.0 88-89 0.0 0.0 0.0 5.17597425691949 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGTG 40 5.3884106E-4 47.499245 9 GTATCAA 12985 0.0 33.05365 1 GGTATCA 8515 0.0 32.040615 1 TAAGGTG 415 0.0 28.620752 5 TAATCGA 35 0.008395724 27.141146 30-31 ATCAACG 16390 0.0 26.088717 3 TATCAAC 16470 0.0 25.99084 2 TCAACGC 16600 0.0 25.730057 4 CAACGCA 16855 0.0 25.368973 5 GTACATG 9300 0.0 24.991915 1 AACGCAG 17205 0.0 24.901075 6 TTAGGGC 630 0.0 24.886427 3 TACATGG 9365 0.0 24.503521 2 GTAAGGT 375 0.0 24.071962 4 GAGTACT 10110 0.0 24.008026 12-13 AGTACTT 10320 0.0 23.841677 12-13 TATTAGG 740 0.0 23.123013 1 ATTAGGG 720 0.0 23.095358 2 ACATGGG 9405 0.0 23.03537 3 GGACCGT 105 0.002196915 22.61762 6 >>END_MODULE