FastQCFastQC Report
Thu 26 May 2016
SRR937457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150279
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143601.2483927812295974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103480.8996078342732502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73760.6412357349825564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24750.21516519035816528No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23070.20056003804294437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.1792608575832472No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17500.1521370032835512No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14610.1270126638841533No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.11092960925132077No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12340.10727832117251553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA123000.034.7580531
GGTATCA77500.033.3195421
TAAGCGG650.00581948529.2559954
TATCAAC156550.027.2983822
ATCAACG156250.027.2583373
TCAACGC157800.026.9941234
CAACGCA159650.026.677835
AACGCAG162500.026.3085846
GTACATG93650.024.5267641
GAGTACT93850.023.96487212-13
TACATGG95050.023.805932
ACGCAGA179450.023.6118357
CGCAGAG179850.023.4800878
AGTACTT95250.023.38822612-13
ACATGGG93950.022.7681163
TAAGGTG4200.022.6356035
GCAGAGT184600.022.5146799
GTACTTT100500.022.14330514-15
CATGGGG27100.021.7530734
TAGAGCG1553.88443E-521.4673165
Produced by FastQC (version 0.11.5)