##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937457_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1150279 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.715582045747162 33.0 31.0 34.0 30.0 34.0 2 31.7643823802747 33.0 31.0 34.0 30.0 34.0 3 31.784568787224664 33.0 31.0 34.0 30.0 34.0 4 35.452102489917664 37.0 35.0 37.0 33.0 37.0 5 35.4511375066397 37.0 35.0 37.0 33.0 37.0 6 35.55368567104155 37.0 35.0 37.0 33.0 37.0 7 35.500269065157234 37.0 35.0 37.0 33.0 37.0 8 35.49829041476025 37.0 35.0 37.0 33.0 37.0 9 37.08514282187191 39.0 37.0 39.0 33.0 39.0 10-11 37.00522568872421 39.0 37.0 39.0 33.0 39.0 12-13 36.89320808256084 39.0 37.0 39.0 33.0 39.0 14-15 37.96721621450101 40.0 38.0 41.0 33.0 41.0 16-17 37.93986893614506 40.0 38.0 41.0 33.0 41.0 18-19 37.98799508640947 40.0 38.0 41.0 33.0 41.0 20-21 37.97641615642814 40.0 38.0 41.0 33.0 41.0 22-23 37.849363936923126 40.0 38.0 41.0 33.0 41.0 24-25 37.69714651836641 40.0 37.0 41.0 32.5 41.0 26-27 37.52244977088168 40.0 37.0 41.0 32.0 41.0 28-29 37.323460221389766 39.5 36.5 41.0 32.0 41.0 30-31 37.17930693336139 39.0 36.5 41.0 32.0 41.0 32-33 36.9199655040212 39.0 35.5 41.0 31.5 41.0 34-35 36.664389248173705 39.0 35.0 41.0 31.0 41.0 36-37 36.58472640116007 39.0 35.0 41.0 31.0 41.0 38-39 36.496986817980684 39.0 35.0 41.0 30.5 41.0 40-41 36.25972220652555 39.0 35.0 41.0 30.0 41.0 42-43 35.92018718936884 39.0 35.0 41.0 29.5 41.0 44-45 35.512649974484454 39.0 35.0 41.0 27.5 41.0 46-47 34.95506611874163 39.0 35.0 40.5 24.5 41.0 48-49 34.46462206125644 38.5 34.5 40.0 22.5 41.0 50-51 33.47237930971529 37.5 33.5 39.5 19.0 40.5 52-53 33.559505998110026 38.0 33.5 39.5 19.0 40.5 54-55 33.87935492171899 38.0 34.0 40.0 18.0 41.0 56-57 33.65894578619622 38.0 34.0 40.0 14.0 41.0 58-59 33.41075121774804 38.0 33.5 40.0 11.5 41.0 60-61 33.128155864794536 37.5 33.0 40.0 8.0 41.0 62-63 32.80740846351189 37.0 33.0 40.0 7.0 41.0 64-65 32.44223314517609 36.0 32.5 39.5 2.0 41.0 66-67 32.05112890003208 36.0 32.0 39.0 2.0 41.0 68-69 31.55631112104107 35.0 31.0 39.0 2.0 40.5 70-71 31.13833165692845 35.0 31.0 38.5 2.0 40.0 72-73 30.74423248620552 35.0 31.0 37.5 2.0 40.0 74-75 30.292046103597475 35.0 30.0 37.0 2.0 39.0 76-77 29.86008394485164 35.0 30.0 36.5 2.0 39.0 78-79 29.394234355317273 34.5 29.0 36.0 2.0 38.5 80-81 28.950198603990856 34.0 29.0 35.5 2.0 37.5 82-83 28.51534671153694 34.0 29.0 35.0 2.0 37.0 84-85 28.065874887744627 34.0 27.5 35.0 2.0 36.5 86-87 27.6242063881893 34.0 26.5 35.0 2.0 36.0 88-89 27.234414433367903 34.0 26.0 35.0 2.0 36.0 90-91 26.792033063282908 33.0 25.0 35.0 2.0 35.0 92-93 26.36021565202877 33.0 24.5 35.0 2.0 35.0 94-95 25.850368475821952 33.0 23.5 35.0 2.0 35.0 96-97 25.26126270235308 32.5 19.0 35.0 2.0 35.0 98-99 24.599377629253425 32.0 8.5 35.0 2.0 35.0 100-101 22.89647815877713 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11044496369009238 1101 2 0.13805402198705252 1101 3 0.23896008486108755 1101 4 0.2098666100876585 1101 5 0.19062936147469145 1101 6 0.07456338772602322 1101 7 0.16368311876719588 1101 8 0.17234491512246564 1101 9 0.17211157895776097 1101 10-11 0.1711834401369856 1101 12-13 0.20431800018339885 1101 14-15 0.37287418297175634 1101 16-17 0.29366639479768963 1101 18-19 0.4094972943540327 1101 20-21 0.32660668924840053 1101 22-23 0.3289077892856511 1101 24-25 0.3675339691678303 1101 26-27 0.4059116313150213 1101 28-29 0.3790696781565188 1101 30-31 0.3685928334865878 1101 32-33 0.3156461294249482 1101 34-35 0.304123779133171 1101 36-37 0.4456957323006421 1101 38-39 0.4164143048197886 1101 40-41 0.4393348956769927 1101 42-43 0.3078056181264657 1101 44-45 0.25544261988969197 1101 46-47 0.48123478317391033 1101 48-49 0.4752046785617381 1101 50-51 0.34667771544603454 1101 52-53 0.5018637160549133 1101 54-55 0.7656195213652097 1101 56-57 0.7604729289459513 1101 58-59 0.8293444317007186 1101 60-61 0.9322398972126749 1101 62-63 1.0272416288742576 1101 64-65 0.7765206447251813 1101 66-67 0.9298879416452337 1101 68-69 0.9081299031915613 1101 70-71 0.6483112179159889 1101 72-73 0.8132392988301014 1101 74-75 0.7284249611391544 1101 76-77 0.816414400616214 1101 78-79 0.9991324816142573 1101 80-81 1.0252731876663859 1101 82-83 1.0115945829759134 1101 84-85 1.056974623181258 1101 86-87 1.1358926243261678 1101 88-89 1.2874201444077755 1101 90-91 1.2196013778274484 1101 92-93 0.9094405616694559 1101 94-95 0.8674211083334207 1101 96-97 0.9996184808862232 1101 98-99 1.0913898870067875 1101 100-101 1.1375230347967253 1102 1 0.18408740312200322 1102 2 0.013576160667085446 1102 3 0.11306200633143249 1102 4 -0.07296547011284105 1102 5 0.08127263632849235 1102 6 0.22961769014874278 1102 7 0.046264572401284454 1102 8 0.16536914235722122 1102 9 0.27988100251665315 1102 10-11 0.15741986453130608 1102 12-13 0.35097631263118245 1102 14-15 0.3172977418690053 1102 16-17 0.2903038216982665 1102 18-19 0.2512475032095054 1102 20-21 0.10937611280730408 1102 22-23 0.14413335319542853 1102 24-25 0.2861638772714201 1102 26-27 0.37086568311117674 1102 28-29 0.23832197556671275 1102 30-31 0.2609696087581028 1102 32-33 0.2722877334349718 1102 34-35 0.3515339712801975 1102 36-37 0.4503949804209455 1102 38-39 0.5933984318039123 1102 40-41 0.580750935777246 1102 42-43 0.428461424308594 1102 44-45 0.43006668004006343 1102 46-47 0.6781854932825198 1102 48-49 0.5723642441423635 1102 50-51 0.6217403720876433 1102 52-53 0.635635645879475 1102 54-55 0.7468642999784052 1102 56-57 0.5824862956961638 1102 58-59 0.7340227942738267 1102 60-61 0.696295870479176 1102 62-63 0.777951737479107 1102 64-65 0.7498915720434738 1102 66-67 0.7002304646193238 1102 68-69 0.8999427686886428 1102 70-71 0.6959093799878318 1102 72-73 0.712403876942048 1102 74-75 0.7244567071709014 1102 76-77 0.6033818191625819 1102 78-79 0.7507615542959662 1102 80-81 0.6272155435561118 1102 82-83 0.7835661786317196 1102 84-85 0.659564431034223 1102 86-87 0.8458132592468033 1102 88-89 0.9197509714754446 1102 90-91 0.7896306175935273 1102 92-93 0.42414398689919963 1102 94-95 0.3863434140978299 1102 96-97 0.3405123823064464 1102 98-99 0.37852438993076376 1102 100-101 0.3035840205945526 1103 1 -0.04286415656818221 1103 2 -0.02524971758787231 1103 3 -0.027841098889247462 1103 4 -0.10101582198121406 1103 5 0.03488548200199659 1103 6 -0.0062544761426863715 1103 7 0.007764367085734136 1103 8 0.10337800632505179 1103 9 0.12449253133870997 1103 10-11 0.11738333252331756 1103 12-13 -0.054748451255932196 1103 14-15 0.02433369332957369 1103 16-17 -0.030930053951301772 1103 18-19 0.09555459863167926 1103 20-21 0.012771607192981094 1103 22-23 -0.09204324649589068 1103 24-25 -0.17064197917760993 1103 26-27 -0.09394039989293645 1103 28-29 -0.0664441771030937 1103 30-31 -0.0019546510439525377 1103 32-33 0.043948523829037356 1103 34-35 -0.022685475642184372 1103 36-37 0.20265699138054316 1103 38-39 0.15742856363065272 1103 40-41 0.12248663094737111 1103 42-43 0.21846609700729402 1103 44-45 0.1645157971831992 1103 46-47 0.048157032299549485 1103 48-49 0.10428727205111699 1103 50-51 0.0070893377220713205 1103 52-53 0.020460702872235004 1103 54-55 0.128963616065235 1103 56-57 0.20611457543505907 1103 58-59 0.17099092080484013 1103 60-61 0.24689553347847948 1103 62-63 0.3498404989307602 1103 64-65 0.40071354222315847 1103 66-67 0.5761349150108401 1103 68-69 0.26949659676165183 1103 70-71 0.20587780387240429 1103 72-73 0.3468147629140397 1103 74-75 0.36166413251391916 1103 76-77 0.31480019561217887 1103 78-79 0.3563291455905855 1103 80-81 0.3986993377874519 1103 82-83 0.4216161057093011 1103 84-85 0.28264048274542475 1103 86-87 0.6096012603229362 1103 88-89 0.49226275941988007 1103 90-91 0.47944130249787875 1103 92-93 0.5443881000068238 1103 94-95 0.38050962057447535 1103 96-97 0.5370276818547488 1103 98-99 0.6199613155782195 1103 100-101 0.972948108781118 1104 1 0.2136574208637434 1104 2 0.28150143059209753 1104 3 0.3549460211380513 1104 4 0.08143697361437319 1104 5 0.1900429452860095 1104 6 0.16841307527013072 1104 7 0.14438848633594858 1104 8 0.27811732918105747 1104 9 0.42552806277036126 1104 10-11 0.3362278383867192 1104 12-13 0.3822978798205128 1104 14-15 0.6948567701321977 1104 16-17 0.5493146605712553 1104 18-19 0.4715743240341439 1104 20-21 0.5135467387794037 1104 22-23 0.5853907678179411 1104 24-25 0.5015794709578785 1104 26-27 0.5275852913476129 1104 28-29 0.4666930244500378 1104 30-31 0.6284768975945738 1104 32-33 0.6869335503485843 1104 34-35 0.7107110165611559 1104 36-37 0.5045988003993429 1104 38-39 0.6370203537605121 1104 40-41 0.4714315581091455 1104 42-43 0.6442028798066701 1104 44-45 0.8611460626788272 1104 46-47 1.0072417637079738 1104 48-49 1.103124248437652 1104 50-51 0.9249296306017527 1104 52-53 0.9805163061666775 1104 54-55 1.1475041372433594 1104 56-57 0.9297361233101995 1104 58-59 0.8048188898752642 1104 60-61 0.7235838424976038 1104 62-63 1.0064578586159527 1104 64-65 1.0394503517737235 1104 66-67 0.86406432844894 1104 68-69 0.7646516981127185 1104 70-71 0.8061421837703975 1104 72-73 0.9660783487588844 1104 74-75 0.8965320045493499 1104 76-77 0.9143856829043919 1104 78-79 0.7575739234025072 1104 80-81 0.8268101241871548 1104 82-83 0.8502935602763451 1104 84-85 0.9492562569341949 1104 86-87 0.8520174655318478 1104 88-89 0.8498561454856493 1104 90-91 1.0646627141377714 1104 92-93 0.7765383789693736 1104 94-95 0.8310613798556616 1104 96-97 1.0919520836887067 1104 98-99 1.0886259034829031 1104 100-101 1.2813852334083151 1105 1 0.10260021943757991 1105 2 0.07793830167715754 1105 3 0.2333295772888313 1105 4 0.22801130250419988 1105 5 0.20459285213721046 1105 6 0.06998501033211113 1105 7 0.11474925866829011 1105 8 0.1228702797255039 1105 9 0.17408694986517048 1105 10-11 0.20005292555986642 1105 12-13 0.19255473647839239 1105 14-15 0.3629598524263642 1105 16-17 0.1848013924860865 1105 18-19 0.29346211594022265 1105 20-21 0.18119032028834425 1105 22-23 0.16016453146472287 1105 24-25 0.11930549860926476 1105 26-27 0.19365522536929092 1105 28-29 0.16171828629056506 1105 30-31 0.2158059493890221 1105 32-33 0.05651809456875867 1105 34-35 0.26214014248933637 1105 36-37 0.20584052278523757 1105 38-39 0.1614156357434453 1105 40-41 0.2188488283462675 1105 42-43 0.4393215401763726 1105 44-45 0.37085016272441607 1105 46-47 0.49392408955254297 1105 48-49 0.45711252260551305 1105 50-51 0.47313297539644594 1105 52-53 0.4359111950053034 1105 54-55 0.7238754509803655 1105 56-57 0.6499383179259084 1105 58-59 0.8663973464235397 1105 60-61 0.6989363345401784 1105 62-63 0.8617475760015694 1105 64-65 0.813532581008964 1105 66-67 0.9573708147591731 1105 68-69 0.9158898886214537 1105 70-71 0.6749272394349219 1105 72-73 0.7740344906932535 1105 74-75 0.8033874851308056 1105 76-77 0.8809291738688536 1105 78-79 0.7208559577066715 1105 80-81 0.7719308180618825 1105 82-83 0.8042485848532159 1105 84-85 0.9684275715274104 1105 86-87 0.8183822221070294 1105 88-89 0.944760538641372 1105 90-91 0.8807660761280651 1105 92-93 0.7690169076535689 1105 94-95 0.6427548899778799 1105 96-97 0.8189329851521876 1105 98-99 0.7851149321881472 1105 100-101 1.2169431023235866 1106 1 -0.06935610928147184 1106 2 -0.06688671136843638 1106 3 -0.2622331607987576 1106 4 -0.11307488289371292 1106 5 -0.11395371469362914 1106 6 -0.11440621141306906 1106 7 0.07603574343288244 1106 8 0.024614297451670097 1106 9 -0.10486700908762714 1106 10-11 -0.09711602654233786 1106 12-13 -0.19641867866893392 1106 14-15 -0.4581474347148671 1106 16-17 -0.3016179901427236 1106 18-19 -0.23286490476289856 1106 20-21 -0.1690401005409612 1106 22-23 0.0030049422711329044 1106 24-25 -0.15371656494411923 1106 26-27 0.179358750275604 1106 28-29 0.22907403017981665 1106 30-31 0.0973189567042354 1106 32-33 0.23133873415045514 1106 34-35 0.0565292610999677 1106 36-37 -0.28663703131328333 1106 38-39 -0.42074168752065333 1106 40-41 -0.09277235400254114 1106 42-43 0.06601867736146971 1106 44-45 0.09455900090537028 1106 46-47 -0.14151205567461744 1106 48-49 -0.040540150443305834 1106 50-51 -0.14148873757584823 1106 52-53 -0.21934369346256943 1106 54-55 -0.13894693798689417 1106 56-57 -0.2802833735750596 1106 58-59 -0.39547634406721244 1106 60-61 -0.40835300661552765 1106 62-63 -0.32981102269484097 1106 64-65 -0.44140296539683277 1106 66-67 -0.446392740119002 1106 68-69 -0.28104226284999 1106 70-71 -0.34976479214905254 1106 72-73 -0.1268357142259653 1106 74-75 -0.3342243923875685 1106 76-77 -0.38849468166076306 1106 78-79 -0.3300165791399863 1106 80-81 -0.27216900955148304 1106 82-83 -0.5391682080161893 1106 84-85 -0.6373614163192904 1106 86-87 -0.6386302356652855 1106 88-89 -0.5556174095968771 1106 90-91 -0.4727734817208926 1106 92-93 -0.6913570455090081 1106 94-95 -0.6332111219598282 1106 96-97 -0.3965762681998477 1106 98-99 -0.434280720219153 1106 100-101 -0.37268166855354323 1107 1 0.0675275435574818 1107 2 0.06773053394204354 1107 3 0.0411099743527501 1107 4 0.10572757050558579 1107 5 0.15860203577648235 1107 6 0.044143456040266926 1107 7 -0.02800866343091002 1107 8 0.08406884520083224 1107 9 0.08696992736040698 1107 10-11 0.1212336711998745 1107 12-13 0.24549240243374726 1107 14-15 0.3490596360559479 1107 16-17 0.18370557502674245 1107 18-19 0.14971730640949232 1107 20-21 0.10244554799865568 1107 22-23 0.13759274005920474 1107 24-25 0.15901980400809634 1107 26-27 0.05043644546008608 1107 28-29 0.15918822346493755 1107 30-31 0.32033082826255566 1107 32-33 0.11825814469519003 1107 34-35 0.09943219785930779 1107 36-37 0.026542628020955306 1107 38-39 -0.018616838439207584 1107 40-41 0.1687200674672269 1107 42-43 0.12547690605092043 1107 44-45 0.21958266409302496 1107 46-47 0.39380761821509935 1107 48-49 0.45758362170018074 1107 50-51 0.1862648161090874 1107 52-53 0.15117555049332765 1107 54-55 0.17852890452775938 1107 56-57 0.17572307963914113 1107 58-59 0.047126227963531164 1107 60-61 0.18310889460065738 1107 62-63 0.33854361802435307 1107 64-65 0.3156255271844941 1107 66-67 0.1684804672630662 1107 68-69 0.13262759256267387 1107 70-71 0.20554449097204497 1107 72-73 0.36973417526168717 1107 74-75 0.43723548956993596 1107 76-77 0.31083875263550453 1107 78-79 0.5619614950761864 1107 80-81 0.57525811834752 1107 82-83 0.7434712154854566 1107 84-85 0.7081048220962671 1107 86-87 0.6873091402996465 1107 88-89 0.7822513680364658 1107 90-91 1.0685214099753289 1107 92-93 0.9657696755935063 1107 94-95 0.7986346908128219 1107 96-97 0.8816859914347468 1107 98-99 1.216630996108659 1107 100-101 1.4682996070289924 1108 1 -0.016227730704123644 1108 2 -0.047037398547406184 1108 3 -0.02218116488775479 1108 4 -0.020221796583022922 1108 5 0.12442482058660431 1108 6 0.15553586110355866 1108 7 0.07958627327794687 1108 8 0.1279507017409145 1108 9 0.30110494845745706 1108 10-11 0.1647990720437562 1108 12-13 0.2814628665687664 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-2.3829589129222697 2316 98-99 -2.360317404981277 2316 100-101 -2.6312666048892446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4338.0 3 781.0 4 614.0 5 689.0 6 826.0 7 1014.0 8 1369.0 9 1802.0 10 2285.0 11 3408.0 12 5094.0 13 6911.0 14 8417.0 15 11274.0 16 15390.0 17 20897.0 18 16270.0 19 9237.0 20 8614.0 21 8640.0 22 9118.0 23 9985.0 24 10941.0 25 12073.0 26 14100.0 27 16136.0 28 19063.0 29 22700.0 30 27734.0 31 34128.0 32 42896.0 33 55470.0 34 75687.0 35 108555.0 36 152076.0 37 208925.0 38 177973.0 39 24849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.174349379146115 19.284982183434302 15.306719702025026 20.23394873539456 2 15.049331420823398 21.49489682473939 40.60296380909198 22.852807945345234 3 18.781680739244138 27.550394883819486 30.97036692759722 22.697557449339158 4 11.879872338199132 17.31352133800729 38.1546591325276 32.651947191265975 5 12.236652588921709 39.157113911160245 33.22702369036866 15.379209809549385 6 29.083042631268974 37.140746265015736 18.030294581964043 15.745916521751251 7 26.469765630377523 35.850039687483935 20.623899445595036 17.0562952365435 8 27.08988808099642 32.922397305927916 20.14829594712927 19.83941866594639 9 27.9038382120522 16.161021246342923 20.64883853441531 35.28630200718957 10-11 24.238150754399356 27.814751304384195 26.65156648089866 21.295531460317793 12-13 26.439748149723002 25.756404737963834 27.984056336948203 19.81979077536496 14-15 23.23463625097735 25.772287427392847 28.233623507837326 22.75945281379247 16-17 20.064075670980056 29.779634235437573 28.389382119367728 21.76690797421464 18-19 20.03227710394665 26.730015746522373 33.28000278776606 19.957704361764918 20-21 21.44000620309292 25.486304625006916 33.21708342245197 19.856605749448192 22-23 20.93111996539598 26.006681224400364 33.30610836756056 19.756090442643096 24-25 20.497514214754073 26.0726945180312 33.572997237629224 19.856794029585505 26-27 20.19974089269176 26.3257904123326 33.395569038506274 20.07889965646936 28-29 19.88109919857183 26.666469181150994 33.58971599985537 19.862715620421802 30-31 20.04873422160957 26.36335792989783 33.53978356707839 20.048124281414207 32-33 19.769054888675118 26.592104437659458 33.13204929803282 20.50679137563261 34-35 20.33792428394631 26.417764744165257 33.1659263383085 20.078384633579933 36-37 20.514874515671657 26.245837335371924 32.93184446607905 20.307443682877373 38-39 20.301115058671137 26.441494590652255 32.904923619286805 20.35246673138981 40-41 20.062607310951968 26.80031952639011 32.8209267136432 20.31614644901472 42-43 20.14579038537845 27.06835100324535 32.161606412128755 20.624252199247444 44-45 20.109685550372884 27.121971917745856 31.885133813496232 20.88320871838503 46-47 20.60087519460143 27.559090445777745 31.0374357826179 20.802598577002925 48-49 20.648291989729373 27.790159609458247 30.395499325195015 21.166049075617366 50-51 20.68493341663334 28.154138119423976 30.13386380449245 21.027064659450236 52-53 20.866471263080705 28.3182408971543 29.718497707486325 21.09679013227867 54-55 20.43760001847333 28.199011764111294 29.812116673005978 21.5512715444094 56-57 20.836066273471708 28.539081261742933 29.281352158608957 21.343500306176402 58-59 21.089406458035484 27.816871507747354 29.55006961098412 21.54365242323304 60-61 20.70607254442024 27.879465432826773 30.25806811263086 21.156393910122127 62-63 20.914135446604295 27.962325567823253 30.269984708807527 20.85355427676492 64-65 20.75406866096742 28.212619925877856 30.139839126212326 20.893472286942398 66-67 20.711403227949134 28.268489057596526 29.90145412335548 21.118653591098855 68-69 20.611383836025933 28.281380108075716 30.001158451820597 21.10607760407775 70-71 20.517558946850816 28.455942463640167 29.806634033228963 21.21986455628005 72-73 20.409910080615127 28.404254346016483 29.65296771431048 21.53286785905791 74-75 20.267548681246506 28.964927899315146 29.43140025331496 21.336123166123393 76-77 20.278081481707343 28.522256537136336 29.72579528080393 21.473866700352392 78-79 20.305314317723074 28.487828633006913 29.681661888371714 21.525195160898303 80-81 20.257723181019003 28.74970547975334 29.537038125836467 21.45553321339119 82-83 20.14373353497902 28.446764841169177 29.656464742204815 21.753036881646988 84-85 20.203597690962656 28.394626762970915 29.37463934900999 22.027136197056436 86-87 20.568338358527534 28.171936986982693 29.20076982810395 22.05895482638582 88-89 20.4306025657052 28.034203595193 29.101759267284844 22.433434571816964 90-91 20.15179482176347 27.782128350509627 29.52607890090305 22.53999792682385 92-93 20.15293722047317 27.682934903242558 29.58515474047888 22.578973135805395 94-95 19.996123532985905 27.470735940729256 29.663988710334273 22.869151815950573 96-97 19.96892859849425 27.458207789017607 29.46635063906739 23.106512973420752 98-99 19.832331302265796 27.7011826188009 29.452700692601436 23.013785386331868 100-101 19.68452912357104 27.32302667392488 30.198497550353835 22.793946652150247 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2763.0 1 1733.0 2 926.0 3 1156.5 4 1014.5 5 1027.5 6 1800.0 7 3672.0 8 5866.5 9 6659.0 10 5565.5 11 4346.0 12 4165.0 13 4166.0 14 4105.0 15 4163.5 16 4176.0 17 4176.5 18 4309.0 19 4557.0 20 4775.5 21 5100.0 22 5681.0 23 6473.5 24 7461.0 25 8481.5 26 9702.5 27 11222.5 28 12415.5 29 13782.0 30 15782.0 31 17845.5 32 20114.5 33 21717.0 34 23109.0 35 24297.0 36 26119.5 37 29148.5 38 30935.0 39 31617.0 40 32900.5 41 34864.0 42 36105.0 43 37639.5 44 40358.0 45 43074.5 46 43153.0 47 43221.0 48 48190.0 49 46902.5 50 39832.5 51 35162.5 52 31550.0 53 30396.5 54 29533.0 55 27071.0 56 24123.5 57 21965.0 58 20798.0 59 19191.5 60 16173.5 61 13441.5 62 11014.0 63 7917.5 64 5443.5 65 4114.0 66 2978.0 67 2241.5 68 1672.0 69 1138.0 70 776.5 71 582.5 72 472.5 73 411.5 74 347.5 75 254.0 76 179.5 77 118.5 78 70.0 79 46.0 80 28.0 81 14.0 82 10.5 83 9.5 84 8.5 85 5.0 86 4.0 87 7.0 88 5.5 89 4.0 90 4.0 91 3.0 92 2.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33678785755455853 2 0.2994056224620288 3 0.303056910540834 4 0.30418707113665466 5 0.28819095193426986 6 0.21586067380174723 7 0.22272857280711897 8 0.2237717979724919 9 0.21777325327159758 10-11 0.2269884088990584 12-13 0.2271622797599539 14-15 0.20921011337249484 16-17 0.22372833025726802 18-19 0.20921011337249484 20-21 0.21425236833846395 22-23 0.21173124085547942 24-25 0.2044721324130928 26-27 0.21664309267577692 28-29 0.2186860752912989 30-31 0.2282489726405507 32-33 0.23011808439517717 34-35 0.21538252893428464 36-37 0.2109488219814497 38-39 0.20103818291040695 40-41 0.2025595529432425 42-43 0.2025160852280186 44-45 0.2211202673438357 46-47 0.21038374168353938 48-49 0.2208159933372686 50-51 0.2277708277730881 52-53 0.23815961171159342 54-55 0.2328565504542811 56-57 0.1954308476465275 58-59 0.18365109682085826 60-61 0.19212730128951325 62-63 0.19408334847458747 64-65 0.18899762579339446 66-67 0.2031246332411528 68-69 0.1908232698327971 70-71 0.19773463655339268 72-73 0.21016640310742002 74-75 0.2000384254602579 76-77 0.20986212910085292 78-79 0.20690632446562965 80-81 0.19347480046145327 82-83 0.2078626142005548 84-85 0.18964964152175254 86-87 0.19390947761369198 88-89 0.2025160852280186 90-91 0.19838665228175079 92-93 0.20490680956533153 94-95 0.2022552789366754 96-97 0.1980389105599598 98-99 0.20521108357189866 100-101 0.1872589171844396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1150279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.6392362874655 #Duplication Level Percentage of deduplicated Percentage of total 1 71.4453571369434 32.607115360153195 2 14.035624222960127 12.811503407075033 3 5.552306741096199 7.602091178921308 4 2.8111627415279092 5.131972824124461 5 1.618759338798792 3.693946997798974 6 1.021880822237036 2.7982716182223366 7 0.6851829989604877 2.188986015279835 8 0.4815514960924695 1.7582114011797385 9 0.355841940464349 1.461631896165839 >10 1.7954045090069672 14.698144731535987 >50 0.12171540845891396 3.8075890520875038 >100 0.06911946805456956 6.089864157280831 >500 0.0032369994306136354 1.0207562943043869 >1k 0.0022849407745508016 1.5417365415676934 >5k 1.9041173121256682E-4 0.6409925444227105 >10k+ 3.8082346242513364E-4 2.147185979880197 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14360 1.2483927812295974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10348 0.8996078342732502 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7376 0.6412357349825564 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2475 0.21516519035816528 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2307 0.20056003804294437 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.1792608575832472 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1750 0.1521370032835512 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1461 0.1270126638841533 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1276 0.11092960925132077 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1234 0.10727832117251553 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.3467715223871775E-5 0.0 16-17 0.0 0.0 0.0 2.173385761193589E-4 0.0 18-19 0.0 0.0 0.0 2.6080629134323066E-4 0.0 20-21 0.0 0.0 0.0 3.0427400656710243E-4 0.0 22-23 0.0 0.0 0.0 6.085480131342049E-4 0.0 24-25 0.0 0.0 0.0 0.0014344346023877686 0.0 26-27 0.0 0.0 0.0 0.0025211274829845627 0.0 28-29 0.0 0.0 0.0 0.005346528972536228 0.0 30-31 0.0 0.0 0.0 0.010997331951639558 0.0 32-33 0.0 0.0 0.0 0.016387328639399658 0.0 34-35 0.0 0.0 0.0 0.023733372512233987 0.0 36-37 0.0 0.0 0.0 0.032209576980888985 0.0 38-39 0.0 0.0 0.0 0.039816427145066544 0.0 40-41 0.0 0.0 0.0 0.046945132441781515 0.0 42-43 0.0 0.0 0.0 0.055464804625660384 0.0 44-45 0.0 0.0 0.0 0.0643756862465541 0.0 46-47 0.0 0.0 0.0 0.0785461614095363 0.0 48-49 0.0 0.0 0.0 0.09580284435341338 0.0 50-51 0.0 0.0 0.0 0.11175549584057433 0.0 52-53 0.0 0.0 0.0 0.13940096272295677 0.0 54-55 0.0 0.0 0.0 0.18582448258205184 0.0 56-57 0.0 0.0 0.0 0.27984515061128645 0.0 58-59 0.0 0.0 0.0 0.3896445992667866 0.0 60-61 0.0 0.0 0.0 0.4935324386518401 0.0 62-63 0.0 0.0 0.0 0.6314989667724091 0.0 64-65 0.0 0.0 0.0 0.7872872581347656 0.0 66-67 0.0 0.0 0.0 1.026837836733523 0.0 68-69 0.0 0.0 0.0 1.3139855635024198 0.0 70-71 4.3467715223871775E-5 0.0 0.0 1.572531533654009 0.0 72-73 8.693543044774355E-5 0.0 0.0 1.8637217579387262 0.0 74-75 8.693543044774355E-5 0.0 0.0 2.174255115498066 0.0 76-77 8.693543044774355E-5 0.0 0.0 2.522431514441279 0.0 78-79 8.693543044774355E-5 0.0 0.0 2.8323563239874847 0.0 80-81 8.693543044774355E-5 0.0 0.0 3.1252852568811567 0.0 82-83 8.693543044774355E-5 0.0 0.0 3.4022615382876675 0.0 84-85 8.693543044774355E-5 0.0 0.0 3.8484141673454877 0.0 86-87 8.693543044774355E-5 0.0 0.0 4.369200863442695 0.0 88-89 8.693543044774355E-5 0.0 0.0 5.028997312825846 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12300 0.0 34.758053 1 GGTATCA 7750 0.0 33.319542 1 TAAGCGG 65 0.005819485 29.255995 4 TATCAAC 15655 0.0 27.298382 2 ATCAACG 15625 0.0 27.258337 3 TCAACGC 15780 0.0 26.994123 4 CAACGCA 15965 0.0 26.67783 5 AACGCAG 16250 0.0 26.308584 6 GTACATG 9365 0.0 24.526764 1 GAGTACT 9385 0.0 23.964872 12-13 TACATGG 9505 0.0 23.80593 2 ACGCAGA 17945 0.0 23.611835 7 CGCAGAG 17985 0.0 23.480087 8 AGTACTT 9525 0.0 23.388226 12-13 ACATGGG 9395 0.0 22.768116 3 TAAGGTG 420 0.0 22.635603 5 GCAGAGT 18460 0.0 22.514679 9 GTACTTT 10050 0.0 22.143305 14-15 CATGGGG 2710 0.0 21.753073 4 TAGAGCG 155 3.88443E-5 21.467316 5 >>END_MODULE