FastQCFastQC Report
Thu 26 May 2016
SRR937457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150279
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154791.3456735279006224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120431.0469633888821754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88960.7733775892631266No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31790.2763677333933767No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.23655130624831022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.22490195856831255No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19740.17161053970384577No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.15778780626265454No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA15020.1305770165325108No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.1131899304429621No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.11188589898624594No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.1090170297814704No Hit
TATTAGGGCATTGTCACCACAATATAGATCTTCCTTGTCCAAGAATGCAA12320.10710445031162005No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12210.10614816057669488No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12140.10553961256356066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147000.030.3311041
GGTATCA99050.030.2815151
GTACATG98150.025.909931
TACATGG98750.025.3508762
CGTATAG1151.2755746E-424.8002551
TATCAAC180550.024.6262252
ACATGGG98250.024.5612583
ATCAACG182450.024.3176983
TCAACGC183900.024.125964
CAACGCA186150.023.8598655
GAGTACT107700.023.61806512-13
AACGCAG188950.023.479116
AGTACTT108950.023.02010212-13
CTAAGGG4550.022.9684012
CATGGGA69350.022.3985924
GTACTTT112950.022.39314314-15
CTATTAC3059.094947E-1221.8188041
CATGGGG29200.021.7992424
AACCGTC1353.795538E-421.111457
ACGCAGA210000.021.0586727
Produced by FastQC (version 0.11.5)