##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937457_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1150279 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07091931609636 31.0 31.0 34.0 31.0 34.0 2 32.26439324720351 33.0 31.0 34.0 31.0 34.0 3 32.31787766272357 34.0 31.0 34.0 30.0 34.0 4 35.98123064056633 37.0 35.0 37.0 35.0 37.0 5 35.893370217138624 37.0 35.0 37.0 35.0 37.0 6 35.99266873515034 37.0 35.0 37.0 35.0 37.0 7 35.94259827398396 37.0 35.0 37.0 35.0 37.0 8 35.928601669681875 37.0 35.0 37.0 35.0 37.0 9 37.577763307858355 39.0 37.0 39.0 35.0 39.0 10-11 37.51308899840821 39.0 37.0 39.0 35.0 39.0 12-13 37.428356511768015 39.0 37.0 39.0 34.5 39.0 14-15 38.508018054750195 40.0 38.0 41.0 34.0 41.0 16-17 38.52342475173414 40.0 38.0 41.0 34.0 41.0 18-19 38.62323662346266 40.0 38.0 41.0 34.0 41.0 20-21 38.57061938886132 40.0 38.0 41.0 34.0 41.0 22-23 38.44995040333693 40.0 38.0 41.0 34.0 41.0 24-25 38.2965071952109 40.0 38.0 41.0 34.0 41.0 26-27 38.17985897334473 40.0 37.5 41.0 34.0 41.0 28-29 38.043900218990345 40.0 37.0 41.0 33.0 41.0 30-31 37.959009075189584 40.0 37.0 41.0 33.0 41.0 32-33 37.74140273794445 40.0 36.0 41.0 33.0 41.0 34-35 37.5604335991529 40.0 36.0 41.0 33.0 41.0 36-37 37.33580592186765 40.0 35.0 41.0 33.0 41.0 38-39 37.142546286596556 40.0 35.0 41.0 32.5 41.0 40-41 36.88891868842255 39.0 35.0 41.0 32.0 41.0 42-43 36.558264994840385 39.0 35.0 41.0 31.0 41.0 44-45 36.05610595342521 39.0 35.0 41.0 30.0 41.0 46-47 35.42476999058489 39.0 35.0 41.0 27.0 41.0 48-49 34.894215664199734 39.0 35.0 41.0 24.0 41.0 50-51 34.58564922075427 39.0 35.0 40.0 23.0 41.0 52-53 34.326558165453775 38.0 34.0 40.0 22.0 41.0 54-55 34.06829864754551 38.0 34.0 40.0 20.0 41.0 56-57 33.80749148684798 38.0 34.0 40.0 19.0 41.0 58-59 33.54065491937173 38.0 33.5 40.0 17.5 41.0 60-61 33.196532754227455 37.0 33.0 40.0 14.0 41.0 62-63 32.82862809805273 36.5 33.0 40.0 9.0 41.0 64-65 32.45490398416384 36.0 32.5 39.0 7.5 41.0 66-67 32.02269058202401 35.5 32.0 39.0 3.5 40.5 68-69 31.619901345673526 35.0 31.0 39.0 2.0 40.0 70-71 31.209351818124127 35.0 31.0 38.0 2.0 40.0 72-73 30.778575023972444 35.0 31.0 37.0 2.0 39.5 74-75 30.338845184516103 35.0 30.0 37.0 2.0 39.0 76-77 29.02684479156796 33.5 28.5 35.5 2.0 38.0 78-79 29.615371140392895 34.0 30.0 36.0 2.0 38.0 80-81 29.457046073170076 34.0 30.0 35.5 2.0 37.0 82-83 29.115924049730545 34.0 29.5 35.0 2.0 37.0 84-85 28.74802243629589 34.0 29.0 35.0 2.0 36.5 86-87 28.337961485865605 34.0 29.0 35.0 2.0 36.0 88-89 27.92078573980747 34.0 28.5 35.0 2.0 36.0 90-91 27.465179752042765 34.0 27.0 35.0 2.0 35.0 92-93 27.097984054303346 33.0 26.0 35.0 2.0 35.0 94-95 26.68279739089386 33.0 25.5 35.0 2.0 35.0 96-97 26.196130677861632 33.0 24.5 35.0 2.0 35.0 98-99 25.738099626264585 33.0 23.5 35.0 2.0 35.0 100-101 23.98723961751888 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14829138277708154 1101 2 0.21776274767712778 1101 3 0.12349058105426991 1101 4 0.016226322366549084 1101 5 0.05489241957120328 1101 6 0.05968848601217758 1101 7 0.19482426674425568 1101 8 0.07077755064064917 1101 9 0.2744022125793464 1101 10-11 0.1210033118889342 1101 12-13 0.11253090959277756 1101 14-15 0.19949172714245123 1101 16-17 0.18331665090799731 1101 18-19 0.1664142336181058 1101 20-21 0.26527699874985444 1101 22-23 0.17638013823341936 1101 24-25 0.23751711008534926 1101 26-27 0.20633666418437002 1101 28-29 -0.016514618953124227 1101 30-31 0.1249755287750034 1101 32-33 0.10319821298502063 1101 34-35 -0.036970288437849774 1101 36-37 -0.13171151895006972 1101 38-39 -0.35589826551633763 1101 40-41 -0.3686556507420775 1101 42-43 -0.19149110590217333 1101 44-45 -0.051474181138644326 1101 46-47 -0.13315551856985763 1101 48-49 -0.1476564385967052 1101 50-51 -0.18293595032536558 1101 52-53 -0.13958070513213272 1101 54-55 -0.04832415675638657 1101 56-57 0.018089401038018593 1101 58-59 -0.11382425115616712 1101 60-61 -0.17885186487447413 1101 62-63 -0.17124046746013022 1101 64-65 -0.016622675743789728 1101 66-67 -0.062375162086063085 1101 68-69 -0.07969230327766397 1101 70-71 -0.27974939668425236 1101 72-73 -0.07421078095823219 1101 74-75 -0.26953693543952184 1101 76-77 -0.1160998293369282 1101 78-79 0.05612703996470714 1101 80-81 0.04195851635784109 1101 82-83 -0.011450267143484183 1101 84-85 -0.02955535213218141 1101 86-87 0.05043770097635303 1101 88-89 -0.0542857486381223 1101 90-91 -0.2261704085026146 1101 92-93 -0.1355048129366061 1101 94-95 -0.06270002241359052 1101 96-97 -0.0611436021123275 1101 98-99 0.0019700182005166766 1101 100-101 0.45245174238066 1102 1 0.2369296450995506 1102 2 0.1524327560313452 1102 3 0.20310628698577204 1102 4 0.09613442595885857 1102 5 0.1737729542412083 1102 6 0.10647211174316595 1102 7 0.13889277133907996 1102 8 0.12445340694808493 1102 9 0.229331201718864 1102 10-11 0.2642990512373018 1102 12-13 0.09686674919177563 1102 14-15 0.19316340634045304 1102 16-17 0.19208858073255186 1102 18-19 0.19068323946606114 1102 20-21 0.2396713178810188 1102 22-23 0.17214037215154576 1102 24-25 0.2355747541956319 1102 26-27 0.2450793542428542 1102 28-29 0.17318880627662026 1102 30-31 0.2446705997858558 1102 32-33 0.0848815880894449 1102 34-35 0.19089103152874287 1102 36-37 0.11636701070740685 1102 38-39 0.033658960882988254 1102 40-41 0.19370859320110867 1102 42-43 0.22939444129917064 1102 44-45 0.09294937775859324 1102 46-47 0.07991883397817645 1102 48-49 -0.0756013007520906 1102 50-51 -0.22704622601459334 1102 52-53 -0.15518220889153156 1102 54-55 -0.21484462459263653 1102 56-57 -0.46685211942982363 1102 58-59 -0.28805148590971186 1102 60-61 -0.14010917481599705 1102 62-63 -0.1610900915202791 1102 64-65 -0.3318900107479692 1102 66-67 -0.2993634661796314 1102 68-69 -0.2852478588332197 1102 70-71 -0.1714996055397222 1102 72-73 -0.07007544261236376 1102 74-75 -0.09278254947461306 1102 76-77 0.009756478098328358 1102 78-79 -0.10020128083730029 1102 80-81 -0.16370146693371979 1102 82-83 -0.2528036506021287 1102 84-85 -0.26660631286734926 1102 86-87 -0.2132005613461203 1102 88-89 0.002021686616682672 1102 90-91 0.006682557245088816 1102 92-93 0.16906912190048828 1102 94-95 0.16100507365992556 1102 96-97 -0.039610602947750095 1102 98-99 -0.12472171112279185 1102 100-101 0.4021175736254392 1103 1 0.11065350276631847 1103 2 0.13134897312775706 1103 3 0.11227185627622305 1103 4 0.018553467914031785 1103 5 -0.05667604962438588 1103 6 -0.062183991792508664 1103 7 -0.02307995709217181 1103 8 -0.08008335869483574 1103 9 0.027442298670280252 1103 10-11 -0.0640854693862849 1103 12-13 -0.007794621749710018 1103 14-15 -0.0672758273369638 1103 16-17 -0.0036620953927837263 1103 18-19 0.05410592046234086 1103 20-21 0.050143826837015126 1103 22-23 -0.025281147749900867 1103 24-25 -0.12105835399858478 1103 26-27 -0.18352747356112076 1103 28-29 -0.16634647259854063 1103 30-31 -0.2392227494063306 1103 32-33 -0.3245459355218401 1103 34-35 -0.2517268125164023 1103 36-37 -0.15432384071493033 1103 38-39 -0.22883342828201592 1103 40-41 -0.1962989114361875 1103 42-43 -0.07787797703480948 1103 44-45 -0.3424682623869657 1103 46-47 -0.2731743509615754 1103 48-49 -0.1391146037836819 1103 50-51 -0.4157312153240227 1103 52-53 -0.15859469490882816 1103 54-55 -0.22866448497806857 1103 56-57 -0.15785221854767428 1103 58-59 -0.2077306272659314 1103 60-61 -0.3127299923966689 1103 62-63 -0.1658194289882431 1103 64-65 -0.3635086052568397 1103 66-67 -0.12994981906749814 1103 68-69 0.06342042203844755 1103 70-71 -0.060466372740208385 1103 72-73 0.2316676155981341 1103 74-75 0.12192795560163816 1103 76-77 0.06607665707684518 1103 78-79 0.10425052664751533 1103 80-81 0.10311670844823695 1103 82-83 0.01830491176423621 1103 84-85 0.09964830673249381 1103 86-87 0.177401650397929 1103 88-89 0.10845301918539008 1103 90-91 0.22748173031901686 1103 92-93 0.5749000834852396 1103 94-95 0.4875488341911982 1103 96-97 0.5196406324854657 1103 98-99 0.441935043758761 1103 100-101 0.6590027243553713 1104 1 0.18126029117040332 1104 2 0.1990718544510699 1104 3 0.1291224862011333 1104 4 0.17780451333035074 1104 5 0.1281739121736294 1104 6 0.22063855722902304 1104 7 0.18860273744269307 1104 8 0.14441641065784694 1104 9 0.22163951224187173 1104 10-11 0.08780182595907604 1104 12-13 0.1966084910430368 1104 14-15 0.07653391831156142 1104 16-17 0.08933607835022173 1104 18-19 0.10611281313075693 1104 20-21 -0.04789041858086307 1104 22-23 -0.018532348453526026 1104 24-25 0.21269418980972432 1104 26-27 0.0379585476453812 1104 28-29 0.10910580167537631 1104 30-31 0.04730842582495853 1104 32-33 -0.06677618744613056 1104 34-35 -0.14209854291937773 1104 36-37 -0.12980149598102741 1104 38-39 -0.14288418896143895 1104 40-41 -0.0695006289920741 1104 42-43 -0.07022485755427255 1104 44-45 -0.1977943233156978 1104 46-47 -0.22681978567793948 1104 48-49 -0.6055262208399697 1104 50-51 -0.6559492569684267 1104 52-53 -0.5192758842007947 1104 54-55 -0.38574411800415476 1104 56-57 -0.3689956485571386 1104 58-59 -0.21243263549616387 1104 60-61 -0.14111174861778153 1104 62-63 -0.18128845863180487 1104 64-65 -0.21417082731377235 1104 66-67 -0.1834312886595697 1104 68-69 -0.1569420498483467 1104 70-71 -0.18551571560740143 1104 72-73 -0.18180147015765158 1104 74-75 -0.1056968076341569 1104 76-77 -0.20741201575824775 1104 78-79 -0.2555380868782713 1104 80-81 -0.08920270318251866 1104 82-83 -0.2329179757575126 1104 84-85 -0.22804183376336695 1104 86-87 -0.13302876294686072 1104 88-89 -0.12594272724740563 1104 90-91 -0.06161233790169618 1104 92-93 -0.17583247458601292 1104 94-95 -0.0012440549551975266 1104 96-97 0.08670289029762301 1104 98-99 0.11603951557640002 1104 100-101 0.5177434748106329 1105 1 -0.0490537747622497 1105 2 0.02453504765330905 1105 3 0.05114577375800877 1105 4 0.07385601737487946 1105 5 0.0165351996605807 1105 6 -0.04000470105965093 1105 7 -0.042383455414920945 1105 8 0.05710055880831533 1105 9 -0.011601373909677193 1105 10-11 -0.0032884966261761406 1105 12-13 -0.04894865722660313 1105 14-15 -0.11686917732524904 1105 16-17 -0.09275894555594988 1105 18-19 -0.0027132408491681304 1105 20-21 -0.07361889500040775 1105 22-23 -0.024435577873560987 1105 24-25 -0.0953297356255689 1105 26-27 0.05375852495574662 1105 28-29 -0.037108561149004515 1105 30-31 -0.09467696005224724 1105 32-33 0.0065594957152370625 1105 34-35 0.1497197744658152 1105 36-37 0.04930320526854359 1105 38-39 0.02749320816639056 1105 40-41 0.19736586819206536 1105 42-43 0.1090118431444651 1105 44-45 0.34638268152404095 1105 46-47 -0.012758062735514386 1105 48-49 -0.08872804228966658 1105 50-51 0.1244849998140225 1105 52-53 0.06504215313150041 1105 54-55 -0.13713564192709526 1105 56-57 -0.14088347613874674 1105 58-59 -0.09499308232499715 1105 60-61 -0.030767344217139225 1105 62-63 -0.024747101063844923 1105 64-65 0.08065208293873383 1105 66-67 0.04454388973589829 1105 68-69 -0.025885861607143568 1105 70-71 -0.042249221562713046 1105 72-73 0.026948523599131136 1105 74-75 0.04663483777108368 1105 76-77 0.10915619834628743 1105 78-79 0.027373835240627642 1105 80-81 0.2539084596184651 1105 82-83 0.44246755182287956 1105 84-85 0.27864804102267016 1105 86-87 0.23774734386852003 1105 88-89 0.32096887807281504 1105 90-91 0.45714085951739136 1105 92-93 0.2282299690020615 1105 94-95 0.32007257176676873 1105 96-97 0.15041617036977328 1105 98-99 0.24331481148705691 1105 100-101 0.2648300329142934 1106 1 -0.10550753792114165 1106 2 0.021915369177705202 1106 3 0.05107449597617375 1106 4 0.12646782009558422 1106 5 -0.1027283688571643 1106 6 -0.18533833827686408 1106 7 -0.07145863059547253 1106 8 0.05584219796850931 1106 9 -0.011251144808802849 1106 10-11 -0.16390505823878243 1106 12-13 -0.28804909642093435 1106 14-15 -0.09286572431270201 1106 16-17 0.05224162202638638 1106 18-19 -0.24426088388651834 1106 20-21 -0.07464846296025485 1106 22-23 -0.024189185541288794 1106 24-25 -0.03678735746437667 1106 26-27 -0.174625607730043 1106 28-29 -0.09240164042838472 1106 30-31 -0.03602282536891721 1106 32-33 -0.1978071043961691 1106 34-35 -0.4486360846998494 1106 36-37 -0.45997830589082866 1106 38-39 -0.4210899919244184 1106 40-41 -0.5604683080083745 1106 42-43 -0.8225487266905986 1106 44-45 -0.7677163277147869 1106 46-47 -0.583054235118631 1106 48-49 -0.9259920666332278 1106 50-51 -0.9722283904652684 1106 52-53 -0.9384456183290482 1106 54-55 -1.2344328940366083 1106 56-57 -1.4926098504139773 1106 58-59 -1.2265076295922839 1106 60-61 -1.1542767806034746 1106 62-63 -0.9175167180733652 1106 64-65 -1.0751356289476703 1106 66-67 -1.287952002991915 1106 68-69 -1.1932381736031985 1106 70-71 -0.9158525950257328 1106 72-73 -1.053405735776316 1106 74-75 -1.1941001505560784 1106 76-77 -1.0999728135588676 1106 78-79 -1.0083165340750782 1106 80-81 -1.2424418539597788 1106 82-83 -1.0170889067801276 1106 84-85 -1.031078560285497 1106 86-87 -0.9973597795977973 1106 88-89 -1.0763022388500296 1106 90-91 -1.1976476434643963 1106 92-93 -1.1598243147486293 1106 94-95 -1.5669305846949975 1106 96-97 -1.247249174284697 1106 98-99 -0.9680018123396081 1106 100-101 -0.6107356794807401 1107 1 0.01313968496715745 1107 2 0.06647683246715985 1107 3 0.07517834476735175 1107 4 0.1314865192723147 1107 5 0.07881245171908091 1107 6 0.06481205442688065 1107 7 0.0448443591694101 1107 8 0.10267427557535314 1107 9 0.05465135865127735 1107 10-11 0.03687130465566213 1107 12-13 0.039871677123414884 1107 14-15 0.09926568735945551 1107 16-17 -0.06891863139058074 1107 18-19 0.17581748859098667 1107 20-21 0.3325313688523295 1107 22-23 0.19404640305157983 1107 24-25 0.16501861701723897 1107 26-27 0.21766376886590422 1107 28-29 0.06821513792853295 1107 30-31 0.16255338692247534 1107 32-33 0.10456952100189199 1107 34-35 0.14413780880949645 1107 36-37 0.09527260803406534 1107 38-39 0.16799665110009698 1107 40-41 0.20385791164490286 1107 42-43 0.25828787742111814 1107 44-45 -0.018447257288933372 1107 46-47 -0.18292445568057047 1107 48-49 -0.28325519286634204 1107 50-51 -0.5513154962365618 1107 52-53 -0.5139879785900376 1107 54-55 -0.47839247100191784 1107 56-57 -0.21859434419003065 1107 58-59 -0.186669338558211 1107 60-61 -0.08519604811738901 1107 62-63 -0.35033429908560976 1107 64-65 -0.16088628836183005 1107 66-67 -0.07855961054899296 1107 68-69 -0.25209535732629007 1107 70-71 -0.17578114271599787 1107 72-73 -0.09202773896506145 1107 74-75 -0.1285483881218603 1107 76-77 0.13126806296767413 1107 78-79 0.2126219163962908 1107 80-81 0.18980360375989136 1107 82-83 0.21136970472712235 1107 84-85 0.21534619498455854 1107 86-87 0.17650259950960745 1107 88-89 0.11861459293310261 1107 90-91 0.13606587841721662 1107 92-93 0.16775518304490689 1107 94-95 0.07441068684901708 1107 96-97 0.19353290984266636 1107 98-99 0.10220108108980597 1107 100-101 0.27151945385343623 1108 1 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-0.2336525421517841 2316 90-91 -0.12550446177835184 2316 92-93 -0.18934536283547132 2316 94-95 -0.2275265505770534 2316 96-97 -0.40760988480970894 2316 98-99 -0.589412956875762 2316 100-101 -0.6875941968013457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 27.0 6 92.0 7 194.0 8 422.0 9 829.0 10 1510.0 11 2556.0 12 3965.0 13 5757.0 14 7680.0 15 11106.0 16 15742.0 17 22425.0 18 18993.0 19 9788.0 20 7508.0 21 7655.0 22 7699.0 23 8226.0 24 9023.0 25 10261.0 26 11992.0 27 14512.0 28 17845.0 29 21684.0 30 26491.0 31 33065.0 32 41198.0 33 53744.0 34 73550.0 35 106693.0 36 154112.0 37 227850.0 38 195728.0 39 20345.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53752053980656 20.05676771947039 15.862602285844519 19.543109454878536 2 15.562224469020125 22.476460058820514 39.41339448951081 22.54792098264856 3 19.27645379946952 27.64659704297827 31.192867121802625 21.88408203574959 4 12.519745209640442 18.048925521547382 37.310774168701684 32.120555100110494 5 12.279379157427938 39.856788835268034 32.65684100691274 15.206991000391287 6 29.277244911886598 37.65929830936668 17.269288581292017 15.794168197454706 7 26.590331563038184 36.24442417882966 20.15102422977382 17.014220028358338 8 27.801168238314354 33.05997936152881 19.57055636067424 19.568296039482593 9 28.22949910413039 16.191463114600893 20.299857686700356 35.27918009456836 10-11 24.607856007107838 28.15403915050175 26.051592700553517 21.186512141836893 12-13 26.913353242135372 26.048407078491977 27.625201587487886 19.413038091884772 14-15 23.404148037128383 26.045116010985158 28.149561975833688 22.401173976052764 16-17 20.1123814309398 29.867753823202893 28.396067388868268 21.623797356989044 18-19 20.019795197512952 26.611630743497884 33.630275785266015 19.738298273723156 20-21 21.34769042988701 25.338200558299334 33.88669183737163 19.427417174442027 22-23 20.935210175389944 25.874287284109627 33.86965878747124 19.320843753029195 24-25 20.61266032530383 25.935675577697054 34.22210360360556 19.229560493393556 26-27 20.303035440202073 26.310104267594802 33.978404041168034 19.40845625103509 28-29 19.90973160736944 26.487770123796277 34.181926934156586 19.420571334677696 30-31 20.025054791055037 26.266714423196458 34.09551074130711 19.612720044441392 32-33 19.810976293577472 26.41459159038807 33.83639969085761 19.93803242517685 34-35 20.37575231748124 26.21111921542513 33.775371018683295 19.63775744841034 36-37 20.731057421721168 26.045029075554716 33.392681253852324 19.831232248871796 38-39 20.37027538536303 26.367907264237633 33.46370750052813 19.798109849871203 40-41 20.228222892011416 26.665921919812497 33.232415787821914 19.873439400354176 42-43 20.286760259432786 26.971732190732396 32.61103471841527 20.130472831419553 44-45 20.359538859702734 27.04409104226018 32.22513842293913 20.371231675097953 46-47 20.9465268860859 27.493329879098898 31.311664387509463 20.24847884730574 48-49 20.981170655119325 27.81925080784749 30.54437227837768 20.655206258655507 50-51 21.017944342198717 28.19650710827547 30.188762900131184 20.596785649394626 52-53 21.19164133223331 28.489001364016904 29.65850024211517 20.660857061634612 54-55 20.711844691592212 28.40071843439722 29.68610224128233 21.201334632728233 56-57 21.173167553263163 28.626620150415683 29.293979982247787 20.906232314073367 58-59 21.456098911655346 27.99142642784924 29.43090328520298 21.12157137529243 60-61 21.025551192362897 28.2138507266498 30.03301807648405 20.727580004503253 62-63 21.12065855327273 28.18077179536443 30.291911788357435 20.40665786300541 64-65 21.095143004436316 28.332995734078427 30.09921940677001 20.47264185471525 66-67 21.026116272660804 28.41958342280438 29.824677317416036 20.729622987118777 68-69 21.007120881107973 28.570372926915994 29.771212027690673 20.65129416428536 70-71 20.87215362533785 28.786407471578634 29.442422229737307 20.899016673346203 72-73 20.77669852270623 28.719032512981634 29.38734863454866 21.116920329763474 74-75 20.698847844740275 29.327580526115838 28.957061721547557 21.01650990759633 76-77 20.646947392762975 28.802794800218035 29.530183546774303 21.02007426024469 78-79 20.670115684977297 28.772932479859236 29.312497228933154 21.244454606230313 80-81 20.59795927770567 29.10589517847409 29.18804916024721 21.10809638357303 82-83 20.399661298090717 28.92941160106201 29.275720393575792 21.395206707271484 84-85 20.380730319935527 28.899868986483963 28.976720975985494 21.74267971759502 86-87 20.707063242917588 28.586542916979273 28.841959211634745 21.8644346284684 88-89 20.679330840604756 28.448924130580494 28.562548738175696 22.30919629063905 90-91 20.3625293326459 28.378612186556936 28.809418441985486 22.449440038811687 92-93 20.31871730650362 28.437136769115344 28.780274492791673 22.46387143158936 94-95 20.233395550123056 28.191030176157263 28.771019900389383 22.804554373330298 96-97 20.3627554706293 28.266272791209783 28.53429472328018 22.83667701488074 98-99 20.250722108360808 28.5583696108113 28.37980400381647 22.811104277011417 100-101 19.89676012509814 28.568596610664642 28.82017533563393 22.714467928603288 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2341.0 1 1621.5 2 1085.0 3 1322.0 4 1179.0 5 1173.5 6 2019.5 7 3896.0 8 5883.0 9 6631.5 10 5706.5 11 4564.5 12 4373.5 13 4496.0 14 4623.5 15 4547.0 16 4499.0 17 4579.5 18 4583.0 19 4790.0 20 5117.5 21 5396.5 22 5826.5 23 6525.5 24 7382.0 25 8391.5 26 9550.5 27 11018.5 28 12279.0 29 13724.0 30 15644.5 31 17544.0 32 19684.0 33 21180.0 34 22668.0 35 23709.5 36 25710.0 37 28919.5 38 30629.5 39 31438.5 40 32651.0 41 34668.0 42 35806.0 43 37186.5 44 40252.5 45 42940.5 46 42471.5 47 42552.0 48 47822.0 49 46503.0 50 39862.0 51 35460.5 52 31543.5 53 30338.0 54 29443.5 55 26980.0 56 24203.0 57 22244.0 58 21391.0 59 19862.0 60 16643.0 61 13698.5 62 11153.5 63 8050.0 64 5463.5 65 4151.0 66 3137.5 67 2455.0 68 1769.0 69 1127.0 70 797.0 71 578.5 72 473.0 73 428.0 74 330.5 75 252.0 76 181.0 77 110.5 78 82.0 79 55.0 80 28.0 81 16.5 82 12.0 83 8.0 84 4.5 85 2.0 86 3.0 87 3.0 88 1.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05928996356536109 2 0.0 3 0.0 4 0.0 5 0.019908213572533272 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0029558046352232805 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001173628311044538 24-25 4.3467715223871775E-5 26-27 0.001521370032835512 28-29 0.0036947557940291004 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013431524004176378 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.3467715223871774E-4 84-85 0.0013909668871638968 86-87 0.0 88-89 0.0 90-91 0.009562897349251791 92-93 0.0010432251653729224 94-95 0.0 96-97 0.0 98-99 1.304031456716153E-4 100-101 0.012344831123579583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1150279.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.39462698115273 #Duplication Level Percentage of deduplicated Percentage of total 1 70.00577479934924 30.378744839443428 2 14.445515087087774 12.537154775095761 3 5.9297417546088935 7.719567946074571 4 2.9831697849011976 5.178141601489324 5 1.7162012205229338 3.723695589459588 6 1.1161907978256043 2.906200998686242 7 0.7204345414221583 2.1884091732546676 8 0.5185067462671268 1.800032547317854 9 0.38514327676494775 1.50418339465624 >10 1.9610731522723444 15.31122291854905 >50 0.1332628615482078 3.98774977619287 >100 0.07751690162133643 6.41210077188028 >500 0.004441072102261307 1.2977850253021828 >1k 0.0024224029648698036 1.8648159040589178 >5k 2.0186691373915032E-4 0.779284210940818 >10k+ 4.0373382747830065E-4 2.4109105275981557 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15479 1.3456735279006224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12043 1.0469633888821754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8896 0.7733775892631266 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3179 0.2763677333933767 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2721 0.23655130624831022 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2587 0.22490195856831255 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1974 0.17161053970384577 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1815 0.15778780626265454 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 1502 0.1305770165325108 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1302 0.1131899304429621 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1287 0.11188589898624594 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1254 0.1090170297814704 No Hit TATTAGGGCATTGTCACCACAATATAGATCTTCCTTGTCCAAGAATGCAA 1232 0.10710445031162005 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1221 0.10614816057669488 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1214 0.10553961256356066 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.3040314567161533E-4 0.0 18-19 0.0 0.0 0.0 1.738708608954871E-4 0.0 20-21 0.0 0.0 0.0 2.173385761193589E-4 0.0 22-23 0.0 0.0 0.0 5.216125826864613E-4 0.0 24-25 0.0 0.0 0.0 0.001347499171940025 0.0 26-27 0.0 0.0 0.0 0.0024341920525368195 0.0 28-29 0.0 0.0 0.0 0.005216125826864613 0.0 30-31 0.0 0.0 0.0 0.010736525660296328 0.0 32-33 0.0 0.0 0.0 0.016126522348056428 0.0 34-35 0.0 0.0 0.0 0.0235595016513385 0.0 36-37 0.0 0.0 0.0 0.03199223840476963 0.0 38-39 0.0 0.0 0.0 0.039859894860290415 0.0 40-41 0.0 0.0 0.0 0.04716247101790087 0.0 42-43 0.0 0.0 0.0 0.055595207771332 0.0 44-45 0.0 0.0 0.0 0.06450608939222571 0.0 46-47 0.0 0.0 0.0 0.07889390313132727 0.0 48-49 0.0 0.0 0.0 0.09628098922087598 0.0 50-51 0.0 0.0 0.0 0.11275525329072339 0.0 52-53 0.0 0.0 0.0 0.1406180587492252 0.0 54-55 0.0 0.0 0.0 0.18721544946921573 0.0 56-57 0.0 0.0 0.0 0.2818011977963607 0.0 58-59 0.0 0.0 0.0 0.3926873393324576 0.0 60-61 0.0 0.0 0.0 0.49757493616766024 0.0 62-63 0.0 0.0 0.0 0.6354545288577814 0.0 64-65 0.0 0.0 0.0 0.7917644328028244 0.0 66-67 0.0 0.0 0.0 1.0340969451759094 0.0 68-69 0.0 0.0 0.0 1.3248524923083878 0.0 70-71 0.0 0.0 0.0 1.5860934608038573 0.0 72-73 0.0 0.0 0.0 1.8799786834324541 0.0 74-75 0.0 0.0 0.0 2.1955108282425395 0.0 76-77 0.0 0.0 0.0 2.547990530992916 0.0 78-79 0.0 0.0 0.0 2.8636530789486727 0.0 80-81 0.0 0.0 0.0 3.1613199928017464 0.0 82-83 0.0 0.0 0.0 3.44359933546557 0.0 84-85 0.0 0.0 0.0 3.8942291391914483 0.0 86-87 0.0 0.0 0.0 4.421666395717908 0.0 88-89 0.0 0.0 0.0 5.094416224237772 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14700 0.0 30.331104 1 GGTATCA 9905 0.0 30.281515 1 GTACATG 9815 0.0 25.90993 1 TACATGG 9875 0.0 25.350876 2 CGTATAG 115 1.2755746E-4 24.800255 1 TATCAAC 18055 0.0 24.626225 2 ACATGGG 9825 0.0 24.561258 3 ATCAACG 18245 0.0 24.317698 3 TCAACGC 18390 0.0 24.12596 4 CAACGCA 18615 0.0 23.859865 5 GAGTACT 10770 0.0 23.618065 12-13 AACGCAG 18895 0.0 23.47911 6 AGTACTT 10895 0.0 23.020102 12-13 CTAAGGG 455 0.0 22.968401 2 CATGGGA 6935 0.0 22.398592 4 GTACTTT 11295 0.0 22.393143 14-15 CTATTAC 305 9.094947E-12 21.818804 1 CATGGGG 2920 0.0 21.799242 4 AACCGTC 135 3.795538E-4 21.11145 7 ACGCAGA 21000 0.0 21.058672 7 >>END_MODULE