FastQCFastQC Report
Thu 26 May 2016
SRR937456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206133
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141641.174331520653195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104370.865327455595693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73760.6115411816109831No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24360.2019677763563388No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23460.1945059126978534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.15703077521301548No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18290.1516416514596649No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16600.1376299297009534No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12870.10670465031634155No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.10330535687192043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85600.034.2491871
GTATCAA132600.033.4689641
ATCAACG163350.027.048833
TATCAAC164350.026.9420662
TCAACGC165000.026.7229774
CAACGCA165950.026.510535
GTACATG100250.026.4951461
AACGCAG169000.026.112086
TACATGG100450.026.0607152
TAGCGCG1309.9988265E-625.5844154
TATTCCG1501.0582426E-625.3386485
GAGTACT95500.025.04271712-13
ACATGGG101550.024.6088053
AGTACTT96200.023.74954812-13
GTACTTT101850.023.36528414-15
ACGCAGA187800.023.3463257
TTAGGGC7150.023.2566263
CGCAGAG188350.023.1772738
ATGGGAT26250.022.9857735
ATGGGAG20950.022.6777865
Produced by FastQC (version 0.11.5)