##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937456_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1206133 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.843777593349987 33.0 31.0 34.0 30.0 34.0 2 31.902809225848227 33.0 31.0 34.0 30.0 34.0 3 31.921754897677122 33.0 31.0 34.0 30.0 34.0 4 35.562392372980426 37.0 35.0 37.0 33.0 37.0 5 35.59520799115852 37.0 35.0 37.0 33.0 37.0 6 35.653515822881886 37.0 35.0 37.0 33.0 37.0 7 35.55831322084712 37.0 35.0 37.0 33.0 37.0 8 35.57550867110012 37.0 35.0 37.0 33.0 37.0 9 37.178561568251595 39.0 37.0 39.0 34.0 39.0 10-11 37.080540039945845 39.0 37.0 39.0 33.0 39.0 12-13 36.93608706502516 39.0 37.0 39.0 33.0 39.0 14-15 38.043622054947505 40.0 38.0 41.0 33.0 41.0 16-17 38.014464408154 40.0 38.0 41.0 33.0 41.0 18-19 38.07294220454958 40.0 38.0 41.0 33.5 41.0 20-21 38.01630251390186 40.0 38.0 41.0 33.0 41.0 22-23 37.87402964681341 40.0 38.0 41.0 33.0 41.0 24-25 37.791295404403996 40.0 37.5 41.0 33.0 41.0 26-27 37.7806087720011 40.0 37.0 41.0 33.0 41.0 28-29 37.673119382356674 40.0 37.0 41.0 33.0 41.0 30-31 37.36529180446932 39.5 36.5 41.0 32.5 41.0 32-33 37.04500830339606 39.0 35.5 41.0 32.0 41.0 34-35 36.98157873136711 39.0 35.0 41.0 31.5 41.0 36-37 36.80142405522442 39.0 35.0 41.0 31.0 41.0 38-39 36.63941994788303 39.0 35.0 41.0 31.0 41.0 40-41 36.355484428334194 39.0 35.0 41.0 30.5 41.0 42-43 35.98660388199311 39.0 35.0 41.0 30.0 41.0 44-45 35.5914459682307 39.0 35.0 41.0 28.0 41.0 46-47 35.024358424817166 39.0 35.0 40.5 25.0 41.0 48-49 34.5297960506843 38.5 35.0 40.0 22.5 41.0 50-51 33.488963489101124 37.5 33.0 39.5 19.5 40.5 52-53 33.56371685377981 38.0 33.5 39.5 19.5 40.5 54-55 33.90457395660346 38.0 34.0 40.0 18.5 41.0 56-57 33.693688009531286 38.0 34.0 40.0 15.5 41.0 58-59 33.42302134175916 38.0 33.5 40.0 12.5 41.0 60-61 33.10589172172555 37.5 33.0 40.0 8.0 41.0 62-63 32.79812715513131 37.0 33.0 40.0 7.0 41.0 64-65 32.37058558218704 36.0 32.0 39.5 3.5 41.0 66-67 32.01195514922484 36.0 32.0 39.0 2.0 41.0 68-69 31.533497549606885 35.0 31.0 39.0 2.0 40.0 70-71 31.071103269705745 35.0 31.0 38.0 2.0 40.0 72-73 30.63110328628766 35.0 30.5 37.5 2.0 40.0 74-75 30.152827258685402 35.0 30.0 37.0 2.0 39.0 76-77 29.67718443985862 34.5 29.5 36.5 2.0 39.0 78-79 29.186912637329385 34.0 29.0 36.0 2.0 38.5 80-81 28.701904350515242 34.0 29.0 35.5 2.0 37.0 82-83 28.23020429753601 34.0 27.5 35.0 2.0 37.0 84-85 27.730315810942905 34.0 26.5 35.0 2.0 36.0 86-87 27.263617279354765 33.5 26.0 35.0 2.0 36.0 88-89 26.804640947557193 33.0 25.0 35.0 2.0 35.5 90-91 26.30588707878816 33.0 24.5 35.0 2.0 35.0 92-93 25.823982098159988 33.0 23.0 35.0 2.0 35.0 94-95 25.322003875194525 32.0 19.5 35.0 2.0 35.0 96-97 24.62901562265521 32.0 12.0 34.5 2.0 35.0 98-99 23.760480394782334 31.5 2.0 34.0 2.0 35.0 100-101 21.358252365203505 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11613642649020761 1101 2 0.03860985247253623 1101 3 0.07923172271302903 1101 4 0.04835079782617413 1101 5 0.11090295588439147 1101 6 0.06338032919762782 1101 7 0.0667048059179649 1101 8 0.005342371425186343 1101 9 0.13683472063854651 1101 10-11 0.0043265096225724164 1101 12-13 -0.028972557746449468 1101 14-15 0.059486933151880805 1101 16-17 -0.06095151961053347 1101 18-19 0.26131237866692203 1101 20-21 0.26391608490805396 1101 22-23 0.12030322806106852 1101 24-25 0.18117598717519456 1101 26-27 0.03823043049927577 1101 28-29 0.06813649805642541 1101 30-31 0.18866850706982063 1101 32-33 0.020401351097348197 1101 34-35 0.04272767989133541 1101 36-37 0.034181794962854894 1101 38-39 0.07041850745816447 1101 40-41 -0.12424877233434017 1101 42-43 0.08796534721046356 1101 44-45 0.1527179861805834 1101 46-47 0.0399094029565461 1101 48-49 0.22696626458449032 1101 50-51 0.31908923846855686 1101 52-53 0.2298464473554347 1101 54-55 0.15922657117977934 1101 56-57 0.09423212709999973 1101 58-59 -0.07750059070701099 1101 60-61 0.13114638979029536 1101 62-63 0.06934061508073341 1101 64-65 0.24607159223884167 1101 66-67 0.2436264921872251 1101 68-69 0.14164368303072905 1101 70-71 -0.053240913910233445 1101 72-73 -0.06278387878768754 1101 74-75 0.12108902225187634 1101 76-77 0.1874545335495199 1101 78-79 0.2550265485151222 1101 80-81 0.2507631023589205 1101 82-83 0.3139233049047192 1101 84-85 0.31670685518252384 1101 86-87 0.4428100041457341 1101 88-89 0.3285415077056264 1101 90-91 0.5902240738249951 1101 92-93 0.4744695613265044 1101 94-95 0.3643926682445482 1101 96-97 0.3838668158825662 1101 98-99 0.6741388520550409 1101 100-101 0.7294787486976873 1102 1 0.05729033047379062 1102 2 0.11664087914769183 1102 3 0.0695358867263316 1102 4 0.05000141163782246 1102 5 0.1268220371127171 1102 6 -0.026324395560031633 1102 7 0.08399700325752235 1102 8 0.059782776859272246 1102 9 -0.05464532606067252 1102 10-11 -0.08855893578886764 1102 12-13 0.06427551414203947 1102 14-15 0.19805447012209498 1102 16-17 0.19790408429522444 1102 18-19 0.04790116908650788 1102 20-21 0.11552170208063473 1102 22-23 0.0830770773853402 1102 24-25 0.1476474104398946 1102 26-27 0.09449723514089214 1102 28-29 0.07703964140885944 1102 30-31 0.009427103970686801 1102 32-33 0.06550965259030761 1102 34-35 0.14169541055715484 1102 36-37 -0.016305650200948207 1102 38-39 0.22287198513451756 1102 40-41 0.15025891800033975 1102 42-43 0.23315468687648888 1102 44-45 0.4662953627106887 1102 46-47 0.4261021787295647 1102 48-49 0.15061736042401463 1102 50-51 0.17141015847846575 1102 52-53 0.24994789432352604 1102 54-55 0.20130782604427822 1102 56-57 0.26649518165317687 1102 58-59 0.32362288803069106 1102 60-61 0.3528184074086411 1102 62-63 0.2521535573081479 1102 64-65 0.32287212114282227 1102 66-67 0.12309575295989106 1102 68-69 0.23339813183848435 1102 70-71 0.13648680667279933 1102 72-73 0.13261264442040854 1102 74-75 0.2252678501723473 1102 76-77 0.27204876778854015 1102 78-79 0.2794966495198601 1102 80-81 0.37119378763451394 1102 82-83 0.25369856493443876 1102 84-85 0.4544070736428658 1102 86-87 0.36828860476131453 1102 88-89 0.2981599317840242 1102 90-91 0.23622821749583522 1102 92-93 0.4045694383872842 1102 94-95 0.4539547746621544 1102 96-97 0.5252049075828431 1102 98-99 0.7078100471303976 1102 100-101 1.0193156994512442 1103 1 0.06391357403350995 1103 2 -0.07076154083669195 1103 3 0.08858838477473441 1103 4 0.12610162631385435 1103 5 0.05808911361545199 1103 6 0.04206699788258561 1103 7 0.11691806102489011 1103 8 0.021702175766378673 1103 9 -0.010587949011487297 1103 10-11 -0.0013224018310253882 1103 12-13 0.01812016675718553 1103 14-15 0.14780076208150206 1103 16-17 -0.06382991258221438 1103 18-19 -0.0033649316160691 1103 20-21 -0.04081977918401236 1103 22-23 -0.13694756156860421 1103 24-25 -0.10690225778805029 1103 26-27 -0.20319499748985947 1103 28-29 0.005013392384661586 1103 30-31 -0.15423897721588276 1103 32-33 -0.0970220609147674 1103 34-35 -0.004961254157912265 1103 36-37 -0.17857511741406285 1103 38-39 -0.2891303567858543 1103 40-41 -0.14318248520028476 1103 42-43 -0.03040283654271292 1103 44-45 -0.16729777741420548 1103 46-47 0.02343824430333541 1103 48-49 -0.01067630663296626 1103 50-51 -0.16812560459725745 1103 52-53 -0.17586689880684503 1103 54-55 -0.023943442495919953 1103 56-57 -0.026025310150103564 1103 58-59 0.009012720193062762 1103 60-61 -0.03365944192781711 1103 62-63 0.011393165036480468 1103 64-65 -0.1339853144543639 1103 66-67 0.017995977393923113 1103 68-69 -0.07862616558540125 1103 70-71 -0.02060482096186078 1103 72-73 -0.07865418813228686 1103 74-75 -0.08790301243498888 1103 76-77 0.06651476115309407 1103 78-79 0.025794707677551543 1103 80-81 -0.013552016424814184 1103 82-83 -0.012475322653383358 1103 84-85 -0.09488913049452918 1103 86-87 0.0065533881336783395 1103 88-89 0.0634616979823086 1103 90-91 0.3327775441937284 1103 92-93 0.4151507713302962 1103 94-95 -0.07505088497875434 1103 96-97 0.11075818627136513 1103 98-99 0.43644002371119583 1103 100-101 0.5224748524567069 1104 1 0.01300362882630779 1104 2 0.004603528662478595 1104 3 0.02769290545799663 1104 4 0.0015395802227899935 1104 5 0.008856989719355113 1104 6 0.0013534706101339111 1104 7 0.09405751442881183 1104 8 0.06672957216507314 1104 9 0.027855802352782177 1104 10-11 0.07500459388840142 1104 12-13 0.05239896257354815 1104 14-15 0.07598834509388297 1104 16-17 0.004670333448913766 1104 18-19 0.1353355800546936 1104 20-21 0.038653825190536395 1104 22-23 -0.04065874692068405 1104 24-25 -0.07790480689253343 1104 26-27 0.23404671607070782 1104 28-29 0.19778340692455032 1104 30-31 0.0018455760988729253 1104 32-33 -0.05541042752704328 1104 34-35 0.2714047735677596 1104 36-37 0.22201350743841175 1104 38-39 0.2583323778224056 1104 40-41 0.33391349653791735 1104 42-43 0.43542103476635674 1104 44-45 0.4054192201920799 1104 46-47 0.4571476537916297 1104 48-49 0.5160522932834155 1104 50-51 0.3647232508955298 1104 52-53 0.4941859331409475 1104 54-55 0.5247781205601925 1104 56-57 0.41758269011627647 1104 58-59 0.3785801493712455 1104 60-61 0.38437519481554006 1104 62-63 0.5602756657486765 1104 64-65 0.790539549488571 1104 66-67 0.5281979449030203 1104 68-69 0.4893681442510349 1104 70-71 0.5875665572934246 1104 72-73 0.5063449424343993 1104 74-75 0.591194700770405 1104 76-77 0.4641417631620399 1104 78-79 0.6041676464731474 1104 80-81 0.7237912543463203 1104 82-83 0.838266100479462 1104 84-85 0.8370573642093255 1104 86-87 0.837936046083815 1104 88-89 0.8535703216508708 1104 90-91 0.970700190526756 1104 92-93 0.8841610232439727 1104 94-95 0.9973775347614513 1104 96-97 0.8793927320017083 1104 98-99 0.7785333601522915 1104 100-101 1.2674996590540424 1105 1 0.05013602644448767 1105 2 0.12659967035648734 1105 3 0.0788078647483097 1105 4 0.10500370101511436 1105 5 -0.04264911673343619 1105 6 0.026045109934472066 1105 7 0.08496998860550775 1105 8 0.07603735561385605 1105 9 0.153573538408196 1105 10-11 0.061967620987687155 1105 12-13 0.03308274857427307 1105 14-15 0.13000273019535769 1105 16-17 0.14954098905712954 1105 18-19 0.16082470388504788 1105 20-21 0.3423131398095691 1105 22-23 0.2512604565062233 1105 24-25 0.265664809707296 1105 26-27 0.23174540363906715 1105 28-29 0.017115190859406937 1105 30-31 0.2070003640439495 1105 32-33 0.05337595295660691 1105 34-35 0.23192549297473164 1105 36-37 0.1548539193961247 1105 38-39 0.28474240637994086 1105 40-41 0.26115063045455145 1105 42-43 0.3192066557409561 1105 44-45 0.14426582974366653 1105 46-47 -0.010882711380325816 1105 48-49 0.17365421939837233 1105 50-51 0.03939894052975035 1105 52-53 0.04499139249202244 1105 54-55 0.02805919967065762 1105 56-57 0.07624792890592857 1105 58-59 0.13955695396475676 1105 60-61 0.24687747334269972 1105 62-63 0.21143125877334512 1105 64-65 0.14372834824905567 1105 66-67 0.23507491962656246 1105 68-69 0.2170290842194369 1105 70-71 0.2770545722405622 1105 72-73 0.1916499612702225 1105 74-75 0.3336698648243619 1105 76-77 0.37458425313652555 1105 78-79 0.2938911092268235 1105 80-81 0.6065990074147329 1105 82-83 0.7693235649344388 1105 84-85 0.796583126756417 1105 86-87 0.7431455186807305 1105 88-89 0.6760502706118636 1105 90-91 0.88249080907093 1105 92-93 1.2450284585338025 1105 94-95 1.1614295915119683 1105 96-97 1.2576339368868723 1105 98-99 1.1320316588520107 1105 100-101 1.3568843807699267 1106 1 -0.23861308249206914 1106 2 -0.13328640853233154 1106 3 -0.048384691361683 1106 4 -0.01494761451855453 1106 5 0.020694018297781724 1106 6 -0.09228136491054073 1106 7 -0.45171414830504375 1106 8 -0.24162608702326338 1106 9 -0.46911307187846063 1106 10-11 -0.8066457492842716 1106 12-13 -0.6421823428002327 1106 14-15 -0.5684286606196665 1106 16-17 -0.3093057183284387 1106 18-19 -0.533810554145326 1106 20-21 -0.3891108875811824 1106 22-23 -0.5482222541627735 1106 24-25 -0.3545960153634482 1106 26-27 -0.5881423223670197 1106 28-29 -0.30167558204362166 1106 30-31 -0.24864020256439545 1106 32-33 -0.16537640971161238 1106 34-35 0.08359043151835976 1106 36-37 -0.432315712081639 1106 38-39 -0.2433142787560385 1106 40-41 -0.10036697209875456 1106 42-43 0.09275942019778682 1106 44-45 0.11869568414929432 1106 46-47 0.042795423002843336 1106 48-49 0.18844249732376284 1106 50-51 -0.14275940414439248 1106 52-53 0.037385963485476736 1106 54-55 0.02868564427351572 1106 56-57 -0.13368459328965798 1106 58-59 -0.03496355939579843 1106 60-61 -0.1422641085379226 1106 62-63 -0.34540475045051267 1106 64-65 -0.09345417664671629 1106 66-67 -0.006346907107506183 1106 68-69 -0.02661297559522069 1106 70-71 0.11106194106522693 1106 72-73 0.07788816143007793 1106 74-75 0.40672457882000046 1106 76-77 0.40584100361715514 1106 78-79 0.21212962530916712 1106 80-81 0.08924487180027896 1106 82-83 -0.28497561366707913 1106 84-85 -0.31598653820372746 1106 86-87 0.027719455345017963 1106 88-89 0.24159068708951636 1106 90-91 0.4002499735234366 1106 92-93 0.41807669206803055 1106 94-95 0.3163993628596877 1106 96-97 0.11612664906797932 1106 98-99 0.18041505374973 1106 100-101 0.42008406808766097 1107 1 0.1287533195802837 1107 2 0.036999623991302855 1107 3 0.08305890526932558 1107 4 0.12038305988164666 1107 5 0.1676958456352864 1107 6 0.15319758669796357 1107 7 0.13600336614750574 1107 8 0.23720152610834333 1107 9 0.19746479048384202 1107 10-11 0.12477559903430802 1107 12-13 0.16513558062447942 1107 14-15 0.2953401043084867 1107 16-17 0.31272521249419327 1107 18-19 0.17007078440951062 1107 20-21 0.3474308319016117 1107 22-23 0.347798769783914 1107 24-25 0.3747008316822402 1107 26-27 0.4245827912304492 1107 28-29 0.4575959061771684 1107 30-31 0.4589082105428304 1107 32-33 0.35291290762655336 1107 34-35 0.6541586775081711 1107 36-37 0.5872705968453928 1107 38-39 0.5293770514167093 1107 40-41 0.37735708222398046 1107 42-43 0.18641454159634208 1107 44-45 0.21846023465190711 1107 46-47 0.2504919220112356 1107 48-49 0.3294769588260351 1107 50-51 0.09054957885974346 1107 52-53 0.15460091406800558 1107 54-55 0.13903373116664142 1107 56-57 0.23127275584517548 1107 58-59 0.2327445739212024 1107 60-61 0.17070446111523552 1107 62-63 0.21567926668777915 1107 64-65 0.23325662292504035 1107 66-67 0.24848342116846567 1107 68-69 0.20553045091413935 1107 70-71 0.290773747479399 1107 72-73 0.243662533783219 1107 74-75 0.4053893258021297 1107 76-77 0.3695656261929585 1107 78-79 0.43517250007455033 1107 80-81 0.5580961715906199 1107 82-83 0.7777997003423422 1107 84-85 0.6648345662336759 1107 86-87 0.9158497681038575 1107 88-89 0.6873490888387686 1107 90-91 0.8722015118808741 1107 92-93 0.9488240015455993 1107 94-95 0.872938063940861 1107 96-97 0.8243495969977168 1107 98-99 0.9791815477912174 1107 100-101 1.4550528885657457 1108 1 0.10208648385976815 1108 2 0.06407148455568645 1108 3 0.20454874865502504 1108 4 0.11250515057058408 1108 5 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-0.5035640538839736 2316 94-95 -0.3979135250137382 2316 96-97 -0.537739183666222 2316 98-99 -0.5608885317776036 2316 100-101 -0.6122872362256473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4726.0 3 1023.0 4 629.0 5 742.0 6 853.0 7 1071.0 8 1321.0 9 1750.0 10 2332.0 11 3308.0 12 5249.0 13 7425.0 14 8530.0 15 11780.0 16 15931.0 17 21837.0 18 17309.0 19 9779.0 20 8947.0 21 9176.0 22 9862.0 23 10880.0 24 11935.0 25 13057.0 26 15228.0 27 17669.0 28 20634.0 29 24646.0 30 29578.0 31 36884.0 32 45519.0 33 59543.0 34 81475.0 35 117251.0 36 162972.0 37 219557.0 38 175301.0 39 20423.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1680719421231 19.24267249226288 15.278972739900595 20.310282825713426 2 15.025674650365195 21.44396327847709 40.60076770487158 22.92959436628614 3 18.779295909354165 27.58146766014128 30.922935918561006 22.716300511943547 4 11.931703449977938 17.22678845503445 38.09202101713563 32.74948707785199 5 12.274912433052314 39.226751650579835 33.17222953966586 15.326106376701997 6 29.243073986918105 37.07965756664176 18.04258721884697 15.634681227593166 7 26.588481142708947 35.86052287712088 20.577439079129803 16.973556901040368 8 27.17267356620666 32.92563324279488 20.096960368093672 19.80473282290479 9 28.058279215652384 16.196824911364462 20.57034245812563 35.17455341485753 10-11 24.27454153518544 27.765718838169306 26.65988429236189 21.299855334283365 12-13 26.47088892509118 25.719672915994803 27.898930776417235 19.910507382496785 14-15 23.267952890180137 25.71622915343933 28.214242975245075 22.80157498113546 16-17 20.139379512234047 29.734115855635107 28.321951795252005 21.804552836878845 18-19 20.08537650056077 26.78293982548222 33.128542204355924 20.003141469601086 20-21 21.583669758621436 25.56501165923456 33.02056446318491 19.830754118959096 22-23 21.03039119264957 26.06276516931571 33.011650310360544 19.89519332767417 24-25 20.612371153723906 26.112734493055417 33.386708968311574 19.8881853849091 26-27 20.39484208851121 26.33546535761788 33.10043533849989 20.16925721537102 28-29 19.96594711204996 26.738124813766778 33.37852108066595 19.917406993517318 30-31 20.230551335033365 26.34650357903976 33.26703327027485 20.155911815652022 32-33 19.873776917782944 26.692865478410916 32.9061896355107 20.527167968295448 34-35 20.40902165139811 26.454779940765594 32.957348067400545 20.178850340435755 36-37 20.715682685727604 26.243000377689796 32.66759101638442 20.373725920198176 38-39 20.442225854622905 26.453525815482728 32.68217426603854 20.42207406385583 40-41 20.256123995191963 26.74086744947551 32.589455805065896 20.41355275026664 42-43 20.23092373649697 27.06481371448326 31.929062501817857 20.775200047201917 44-45 20.223104841072505 27.158289152751014 31.629030227040474 20.989575779136015 46-47 20.72574779619878 27.47945820362666 30.885177904217247 20.909616095957308 48-49 20.690968005260274 27.80942144459712 30.22542371895104 21.274186831191567 50-51 20.87397203109946 28.047871159845684 29.923464998142112 21.154691810912745 52-53 21.013890863558245 28.244340181902384 29.57543361660485 21.166335337934523 54-55 20.53324610626601 28.137254616723904 29.669976612450387 21.659522664559695 56-57 21.003843357224472 28.491368027422876 29.082040095564558 21.422748519788097 58-59 21.25440765444654 27.78151659870321 29.328308358009554 21.635767388840698 60-61 20.838039628005053 27.862399921569832 30.00755638102937 21.29200406939574 62-63 20.97039542013909 27.956054273678266 30.11312555565711 20.96042475052554 64-65 20.840641094556545 28.241260388375487 29.916693599107365 21.0014049179606 66-67 20.8266296118592 28.182008893984616 29.752642543654677 21.238718950501507 68-69 20.757864038950462 28.22620847804051 29.814511706741552 21.201415776267467 70-71 20.664775064080462 28.412881730525562 29.532887712753364 21.389455492640614 72-73 20.53285330477313 28.456481901923453 29.406762304020766 21.603902489282653 74-75 20.393386611361215 28.942009043637796 29.23706562038325 21.42753872461774 76-77 20.447026540538786 28.510407378198316 29.465798630299776 21.576767450963118 78-79 20.455388701204864 28.485476497440814 29.390570058537747 21.668564742816567 80-81 20.43326417215566 28.708466940153837 29.260786941749227 21.597481945941276 82-83 20.33156355418183 28.42568589328149 29.36474899047809 21.878001562058593 84-85 20.3060677202666 28.37254247836251 29.12200790262267 22.199381898748218 86-87 20.690049163987847 28.141262226077895 29.008414757422894 22.160273852511363 88-89 20.575370330823606 27.977541254276243 28.90238156767496 22.54470684722519 90-91 20.256337701611645 27.7934772085401 29.28242313338477 22.66776195646348 92-93 20.338568996333127 27.669618945771298 29.290838129852087 22.700973928043496 94-95 20.093177804339074 27.45891065724253 29.459815513929932 22.988096024488463 96-97 20.109483061287662 27.43200935695841 29.281379777419065 23.177127804334866 98-99 20.0440288838786 27.670424939395875 29.149190435310867 23.136355741414658 100-101 20.060169041607363 27.08898820131787 29.98993028466886 22.860912472405907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2718.0 1 1738.5 2 920.0 3 1127.0 4 1009.0 5 1033.0 6 1773.5 7 3598.5 8 5672.0 9 6341.0 10 5338.0 11 4309.0 12 4158.0 13 4238.0 14 4244.0 15 4155.5 16 4278.5 17 4422.0 18 4436.5 19 4607.5 20 4859.5 21 5261.0 22 5832.0 23 6591.0 24 7501.5 25 8511.0 26 9873.0 27 11536.0 28 12712.5 29 14199.0 30 16287.5 31 18480.5 32 20940.0 33 22583.0 34 24089.5 35 25425.0 36 27412.0 37 30480.5 38 32308.5 39 32924.5 40 34595.5 41 37008.5 42 37849.5 43 39292.0 44 42749.0 45 45561.0 46 45476.5 47 46015.0 48 51011.0 49 49643.5 50 42164.5 51 37182.5 52 33671.0 53 32230.5 54 31329.5 55 28727.5 56 25672.0 57 23293.0 58 21826.0 59 20249.0 60 17212.5 61 14319.5 62 11679.5 63 8381.5 64 5809.0 65 4437.5 66 3215.0 67 2457.5 68 1801.5 69 1192.5 70 851.0 71 618.0 72 480.5 73 412.5 74 324.0 75 257.5 76 193.5 77 116.5 78 65.0 79 46.5 80 37.5 81 26.0 82 19.0 83 15.0 84 14.0 85 10.0 86 5.0 87 3.5 88 4.0 89 3.5 90 3.5 91 2.5 92 1.5 93 2.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2624917815862762 2 0.23264432695233442 3 0.22219771783045486 4 0.2266748360255461 5 0.22949376229652949 6 0.2566881098518986 7 0.256771019448104 8 0.24549531436417046 9 0.2396916426297929 10-11 0.249930977761159 12-13 0.25013825175167254 14-15 0.23355633251059377 16-17 0.24997243255926171 18-19 0.23832363429240389 20-21 0.2317323213940751 22-23 0.23003267467186453 24-25 0.22489227970713013 26-27 0.23351487771249108 28-29 0.24910188179910506 30-31 0.25013825175167254 32-33 0.25080152852131565 34-35 0.2583463017760064 36-37 0.22920357870981062 38-39 0.22932794310411872 40-41 0.2264675620350326 42-43 0.23148359260545892 44-45 0.25051134493459676 46-47 0.25685392904430937 48-49 0.2614968664318114 50-51 0.2614968664318114 52-53 0.28189262709833823 54-55 0.2605434060754494 56-57 0.22866466633447557 58-59 0.21100492234272672 60-61 0.2084761796584622 62-63 0.21581367892263953 64-65 0.20760562889830558 66-67 0.20814454127364063 68-69 0.21166819911236986 70-71 0.21291184305545074 72-73 0.21502603775868828 74-75 0.21846678600121214 76-77 0.23247850775992368 78-79 0.22563846607297872 80-81 0.22087116429116857 82-83 0.2149845829605856 84-85 0.21626968170176922 86-87 0.22480937011092475 88-89 0.22236353702286563 90-91 0.232022504980794 92-93 0.22020788752152543 94-95 0.21784496402967168 96-97 0.2280428443629351 98-99 0.23479997645367467 100-101 0.20926382082241346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1206133.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.81826602983931 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6062736541237 31.644507560061502 2 14.273423168524198 12.794201534467788 3 5.78683848696418 7.780682003414202 4 2.8965420919322424 5.19271976171386 5 1.7101132476010628 3.8322155236068447 6 1.0670604307459566 2.869427895305232 7 0.7215373601680269 2.2636637350935027 8 0.5120811422930955 1.836047109132476 9 0.3613783638814014 1.4576716484878247 >10 1.8622544485208967 15.010058343822386 >50 0.12274447453757788 3.7507817283640943 >100 0.07290657670462944 6.167119481025867 >500 0.003885882001720327 1.16702777043329 >1k 0.0024055460010649642 1.5819396475991336 >5k 1.8504200008192032E-4 0.6117109746102862 >10k+ 3.7008400016384064E-4 2.0402252828616665 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14164 1.174331520653195 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10437 0.865327455595693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7376 0.6115411816109831 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2436 0.2019677763563388 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2346 0.1945059126978534 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1894 0.15703077521301548 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1829 0.1516416514596649 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1660 0.1376299297009534 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1287 0.10670465031634155 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1246 0.10330535687192043 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.14547981026968E-5 0.0 18-19 0.0 0.0 0.0 1.243643943080904E-4 0.0 20-21 0.0 0.0 0.0 2.07273990513484E-4 0.0 22-23 0.0 0.0 0.0 2.901835867188776E-4 0.0 24-25 0.0 0.0 0.0 6.21821971540452E-4 0.0 26-27 0.0 0.0 0.0 0.001243643943080904 0.0 28-29 0.0 0.0 0.0 0.00414547981026968 0.0 30-31 0.0 0.0 0.0 0.009741877554133749 0.0 32-33 0.0 0.0 0.0 0.01654046444297602 0.0 34-35 0.0 0.0 0.0 0.024707059669207294 0.0 36-37 0.0 0.0 0.0 0.03473912081005992 0.0 38-39 0.0 0.0 0.0 0.04497845594142603 0.0 40-41 0.0 0.0 0.0 0.05430578551453281 0.0 42-43 0.0 0.0 0.0 0.06529130701174746 0.0 44-45 0.0 0.0 0.0 0.07495027496967581 0.0 46-47 0.0 0.0 0.0 0.09381220810640287 0.0 48-49 0.0 0.0 0.0 0.11843635817940476 0.0 50-51 0.0 0.0 0.0 0.14086340395296373 0.0 52-53 0.0 0.0 0.0 0.17871163462072592 0.0 54-55 0.0 0.0 0.0 0.23936000424497134 0.0 56-57 0.0 0.0 0.0 0.34710102451388036 0.0 58-59 0.0 0.0 0.0 0.46441810314451226 0.0 60-61 0.0 0.0 0.0 0.5844711984499222 0.0 62-63 0.0 0.0 0.0 0.7306822713581338 0.0 64-65 0.0 0.0 0.0 0.8973305597309749 0.0 66-67 0.0 0.0 0.0 1.1351567364461466 0.0 68-69 0.0 0.0 0.0 1.4289468905999587 0.0 70-71 0.0 0.0 0.0 1.7050773007620221 0.0 72-73 8.29095962053936E-5 0.0 0.0 1.9996965508778883 0.0 74-75 8.29095962053936E-5 0.0 0.0 2.31131226821586 0.0 76-77 8.29095962053936E-5 0.0 0.0 2.6647144220413503 0.0 78-79 8.29095962053936E-5 0.0 0.0 2.9969331740363625 0.0 80-81 8.29095962053936E-5 0.0 0.0 3.3271620957224455 0.0 82-83 8.29095962053936E-5 0.0 0.0 3.6440840272175623 0.0 84-85 8.29095962053936E-5 0.0 0.0 4.113227977345782 0.0 86-87 8.29095962053936E-5 0.0 0.0 4.667229899190222 0.0 88-89 8.29095962053936E-5 0.0 0.0 5.314919664746757 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8560 0.0 34.249187 1 GTATCAA 13260 0.0 33.468964 1 ATCAACG 16335 0.0 27.04883 3 TATCAAC 16435 0.0 26.942066 2 TCAACGC 16500 0.0 26.722977 4 CAACGCA 16595 0.0 26.51053 5 GTACATG 10025 0.0 26.495146 1 AACGCAG 16900 0.0 26.11208 6 TACATGG 10045 0.0 26.060715 2 TAGCGCG 130 9.9988265E-6 25.584415 4 TATTCCG 150 1.0582426E-6 25.338648 5 GAGTACT 9550 0.0 25.042717 12-13 ACATGGG 10155 0.0 24.608805 3 AGTACTT 9620 0.0 23.749548 12-13 GTACTTT 10185 0.0 23.365284 14-15 ACGCAGA 18780 0.0 23.346325 7 TTAGGGC 715 0.0 23.256626 3 CGCAGAG 18835 0.0 23.177273 8 ATGGGAT 2625 0.0 22.985773 5 ATGGGAG 2095 0.0 22.677786 5 >>END_MODULE