FastQCFastQC Report
Thu 26 May 2016
SRR937456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206133
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160961.3345128605220156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128601.0662174072013617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92010.7628511946858265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30090.24947497498202936No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.23123486381684275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26400.2188813339822391No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19800.16416100048667934No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18080.14990054993935162No Hit
CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA15320.12701750138666298No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14460.11988727611299914No Hit
TATTAGGGCATTGTCACCACAATATAGATCTTCCTTGTCCAAGAATGCAA13180.10927484779870876No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13150.10902611901009258No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.10786538466321707No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.10769956547080628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98350.035.948861
GTATCAA146200.031.0414921
CGACCGT701.7078492E-830.53535518-19
TATCACG1056.908616E-527.1414132
TATCAAC181450.024.9986692
ATCAACG182250.024.8889353
TCAACGC184300.024.5863194
CAACGCA186650.024.2767685
GAGTACT112300.024.19584312-13
AACGCAG189400.023.8992046
CGATCCG701.7812674E-523.74873570-71
GCGACAG1452.3331835E-522.9326677
GTACTTT119950.022.53110514-15
AGTACTT113400.022.45309612-13
GTACATG103300.022.3574641
TACATGG104150.021.8447322
ACGCAGA210650.021.4233137
CGCAGAG211300.021.2899688
ACATGGG105500.020.6647783
GCAGAGT218500.020.4144949
Produced by FastQC (version 0.11.5)