##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937456_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1206133 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18827940202283 33.0 31.0 34.0 31.0 34.0 2 32.36635180365681 34.0 31.0 34.0 31.0 34.0 3 32.39019991990933 34.0 31.0 34.0 30.0 34.0 4 36.07264373000324 37.0 35.0 37.0 35.0 37.0 5 36.01250939987547 37.0 35.0 37.0 35.0 37.0 6 36.07726013631996 37.0 35.0 37.0 35.0 37.0 7 36.017465735536625 37.0 35.0 37.0 35.0 37.0 8 36.00429305889151 37.0 35.0 37.0 35.0 37.0 9 37.67540727266396 39.0 37.0 39.0 35.0 39.0 10-11 37.606649100886884 39.0 37.0 39.0 35.0 39.0 12-13 37.50619459048049 39.0 37.0 39.0 35.0 39.0 14-15 38.60367554821897 40.0 38.0 41.0 34.0 41.0 16-17 38.60623621109778 40.0 38.0 41.0 34.5 41.0 18-19 38.69777379443229 40.0 38.0 41.0 34.5 41.0 20-21 38.6615800247568 40.0 38.0 41.0 34.0 41.0 22-23 38.5172302722834 40.0 38.0 41.0 34.0 41.0 24-25 38.426343114731125 40.0 38.0 41.0 34.0 41.0 26-27 38.32111052429541 40.0 38.0 41.0 34.0 41.0 28-29 38.167872448560814 40.0 37.5 41.0 34.0 41.0 30-31 38.08183964786636 40.0 37.0 41.0 34.0 41.0 32-33 37.88740711016115 40.0 36.5 41.0 33.5 41.0 34-35 37.69726058403178 40.0 36.0 41.0 33.0 41.0 36-37 37.465000543057855 40.0 36.0 41.0 33.0 41.0 38-39 37.27999068096139 40.0 35.0 41.0 33.0 41.0 40-41 37.013428867297385 40.0 35.0 41.0 32.5 41.0 42-43 36.692962136016504 39.5 35.0 41.0 31.0 41.0 44-45 36.22396991044934 39.0 35.0 41.0 30.5 41.0 46-47 35.60394210257078 39.0 35.0 41.0 28.0 41.0 48-49 35.063136072058384 39.0 35.0 41.0 24.5 41.0 50-51 34.74137222014488 39.0 35.0 40.5 23.5 41.0 52-53 34.46073525888107 38.5 34.5 40.0 22.5 41.0 54-55 34.20805499890974 38.0 34.0 40.0 21.5 41.0 56-57 33.93605224299476 38.0 34.0 40.0 20.0 41.0 58-59 33.6590127291103 38.0 34.0 40.0 18.5 41.0 60-61 33.32518387275698 37.0 33.0 40.0 16.5 41.0 62-63 32.959370566927525 37.0 33.0 40.0 12.0 41.0 64-65 32.551129518883904 36.0 32.5 39.0 8.0 41.0 66-67 32.13722698906339 36.0 32.0 39.0 6.5 40.5 68-69 31.701829317330677 35.0 31.5 39.0 2.0 40.0 70-71 31.29021716510534 35.0 31.0 38.0 2.0 40.0 72-73 30.84308446912571 35.0 31.0 37.0 2.0 39.5 74-75 30.37727389931293 35.0 30.5 37.0 2.0 39.0 76-77 29.118443820043062 33.5 28.5 35.5 2.0 38.0 78-79 29.669014528248542 34.0 30.0 36.0 2.0 38.0 80-81 29.46288717745058 34.0 30.0 35.5 2.0 37.0 82-83 29.114125473724705 34.0 29.5 35.0 2.0 37.0 84-85 28.767497033909194 34.0 29.0 35.0 2.0 36.5 86-87 28.34019050967016 34.0 29.0 35.0 2.0 36.0 88-89 27.87096862452151 34.0 28.0 35.0 2.0 35.5 90-91 27.374999274541032 33.5 27.0 35.0 2.0 35.0 92-93 27.06500526890484 33.0 26.0 35.0 2.0 35.0 94-95 26.665529837920033 33.0 25.0 35.0 2.0 35.0 96-97 26.2145866168988 33.0 25.0 35.0 2.0 35.0 98-99 25.542063769086823 33.0 22.0 35.0 2.0 35.0 100-101 23.30480593765364 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03431753471001997 1101 2 0.04543981225754834 1101 3 -0.005758966277028321 1101 4 0.027245969083345756 1101 5 -0.02812451545000272 1101 6 -0.0949150479826173 1101 7 -0.08322712665529508 1101 8 -0.015893663136523628 1101 9 0.021860690779028857 1101 10-11 -0.09820742383042358 1101 12-13 -0.12039919918950659 1101 14-15 -0.13142477434015376 1101 16-17 -0.07247741005051722 1101 18-19 -0.07820438331866342 1101 20-21 0.011408677347098717 1101 22-23 -0.11469154007073001 1101 24-25 -0.04181547485671189 1101 26-27 -0.12161285346554251 1101 28-29 -0.11363474148712527 1101 30-31 -0.05535005854048336 1101 32-33 0.08188888347472556 1101 34-35 -0.033675487383540315 1101 36-37 0.006967637822512529 1101 38-39 0.021176406348594412 1101 40-41 0.14126730186712422 1101 42-43 -0.015216915166668343 1101 44-45 -0.06699381274488303 1101 46-47 0.021295704085297018 1101 48-49 -0.0014014429825266461 1101 50-51 0.061593048471742406 1101 52-53 0.19285893228438056 1101 54-55 0.07360556299070709 1101 56-57 0.10821366789365783 1101 58-59 0.09838717390709206 1101 60-61 0.0677820343785811 1101 62-63 0.06763175511147779 1101 64-65 0.10877055647571154 1101 66-67 0.2169888514600089 1101 68-69 0.0011505538534422044 1101 70-71 0.06170275773425615 1101 72-73 0.2103341059183741 1101 74-75 0.16870861449817554 1101 76-77 0.2582194695430111 1101 78-79 0.19233684646487248 1101 80-81 0.23211822550183925 1101 82-83 -0.01011513647075546 1101 84-85 0.08340636577250393 1101 86-87 -0.050603050756851786 1101 88-89 0.14643300262433812 1101 90-91 0.15511781968109162 1101 92-93 0.0205407709598866 1101 94-95 0.1344244761872062 1101 96-97 0.2180924840851297 1101 98-99 0.20350107202493817 1101 100-101 0.726613770655792 1102 1 -0.11816606297719545 1102 2 -0.0521608829239355 1102 3 -0.03271051584187035 1102 4 0.0259093367335268 1102 5 0.040322337659745244 1102 6 -0.00861989656394968 1102 7 -0.11233564041740607 1102 8 -0.12974179339902747 1102 9 -0.18544778052925892 1102 10-11 -0.08442651213439234 1102 12-13 -0.05805287321484087 1102 14-15 -0.062269804588517275 1102 16-17 -0.12113624486972441 1102 18-19 -0.06907736233939943 1102 20-21 0.01092886060614262 1102 22-23 0.05890814683227319 1102 24-25 -0.023730032098619347 1102 26-27 -0.06772412672670214 1102 28-29 -0.18049565654407473 1102 30-31 -0.052326881401874914 1102 32-33 0.05469631997086566 1102 34-35 -0.11710776910008747 1102 36-37 -0.19394677790663195 1102 38-39 -0.018895477717066456 1102 40-41 0.03847319852742004 1102 42-43 -0.03877326378337642 1102 44-45 -0.042551350953331735 1102 46-47 0.044921703519243295 1102 48-49 -0.02474994471606351 1102 50-51 0.03302792164091528 1102 52-53 0.08707426607762159 1102 54-55 -0.0323559941804632 1102 56-57 -0.09709538273254026 1102 58-59 -0.07126839727383327 1102 60-61 0.045286478764055005 1102 62-63 -0.06440463578216793 1102 64-65 -0.22135441699064273 1102 66-67 -0.04773787411134833 1102 68-69 -0.04991012859189681 1102 70-71 -0.08027877484905943 1102 72-73 -0.031761631893836295 1102 74-75 -0.016489039043179332 1102 76-77 -0.05933187305940635 1102 78-79 -0.08024273235773904 1102 80-81 -0.1225282579057918 1102 82-83 0.14478828106267727 1102 84-85 0.03966454697960842 1102 86-87 -0.08402051785513365 1102 88-89 0.20412101560813412 1102 90-91 0.10343857164457759 1102 92-93 -0.016155259606872363 1102 94-95 0.21560526759738252 1102 96-97 0.11754047371931975 1102 98-99 0.2633781106933064 1102 100-101 0.9610202793588272 1103 1 0.03515653655442463 1103 2 0.009375514421883224 1103 3 -0.03990704199331674 1103 4 -0.09951757583478127 1103 5 -0.05719669044330544 1103 6 -0.033734553082297225 1103 7 -0.03323484026127943 1103 8 -0.09191733984711448 1103 9 -0.0798052161264522 1103 10-11 -0.06019007575655877 1103 12-13 0.00706958970474858 1103 14-15 -0.04852931864003551 1103 16-17 -0.224442987648807 1103 18-19 -0.006254128537683812 1103 20-21 -0.09394526117372237 1103 22-23 0.00988638648878748 1103 24-25 -0.04437430415170951 1103 26-27 -0.10298710486878093 1103 28-29 -0.24906391376499926 1103 30-31 -0.29379472878672885 1103 32-33 -0.18276464802288928 1103 34-35 0.07868043347602338 1103 36-37 -0.15848254098235515 1103 38-39 -0.15297651792003109 1103 40-41 -0.2555292897629684 1103 42-43 -0.013609817257226098 1103 44-45 0.061340871919419726 1103 46-47 -0.016382941289499797 1103 48-49 -0.028811712865945083 1103 50-51 -0.0919781771100574 1103 52-53 -0.08225755476546226 1103 54-55 -0.14801880643651089 1103 56-57 -0.1485383472134174 1103 58-59 -0.25001668962355694 1103 60-61 -0.15478304699706769 1103 62-63 -0.22791824819434225 1103 64-65 -0.336084120347401 1103 66-67 -0.025020026726494393 1103 68-69 -0.12010675232335899 1103 70-71 -0.03783146805748672 1103 72-73 -0.11284452611709739 1103 74-75 0.008338976179299351 1103 76-77 0.1607232596494157 1103 78-79 0.012183838680510206 1103 80-81 -0.16714672004474096 1103 82-83 -0.32066839341039 1103 84-85 -0.30800085114685416 1103 86-87 -0.1571018507981492 1103 88-89 -0.13728096526618216 1103 90-91 -0.18688027105643457 1103 92-93 -0.3488055328309194 1103 94-95 -0.301776172683649 1103 96-97 -0.20666302940324144 1103 98-99 -0.014743230056108558 1103 100-101 0.23861712752432496 1104 1 0.0875648668354927 1104 2 0.02252245605146186 1104 3 -0.00411228745099379 1104 4 -0.01529538567676525 1104 5 0.034042011548251594 1104 6 -0.014166753932492782 1104 7 0.019558260507892555 1104 8 -0.011529905563328668 1104 9 -0.021506937604414134 1104 10-11 0.05294724661081318 1104 12-13 0.005381594174018289 1104 14-15 -0.012332219957507107 1104 16-17 -0.1774528917891658 1104 18-19 0.01474083614852617 1104 20-21 -0.09901387241124127 1104 22-23 -0.11491336637016758 1104 24-25 -0.04317929580884794 1104 26-27 -0.17330483649876527 1104 28-29 -0.014063488297630045 1104 30-31 -0.13471340532424136 1104 32-33 -0.05821555093863395 1104 34-35 0.009516088381296584 1104 36-37 -0.15429538713706137 1104 38-39 -0.13859017135281704 1104 40-41 -0.07374928228954758 1104 42-43 -0.06568239829025657 1104 44-45 -0.07842608295513998 1104 46-47 4.692661461902503E-4 1104 48-49 0.02686439177682587 1104 50-51 -0.18103034680864027 1104 52-53 -0.1665278412344975 1104 54-55 -0.05435124073879649 1104 56-57 0.12763344541009047 1104 58-59 0.02754712123214631 1104 60-61 -0.11457268113213104 1104 62-63 -0.015524938761181772 1104 64-65 0.057018128710097926 1104 66-67 0.1421793990775484 1104 68-69 0.07696233247783724 1104 70-71 0.2252673772610727 1104 72-73 0.16446770403484834 1104 74-75 0.01568661261558546 1104 76-77 0.18471221969617702 1104 78-79 0.07355473153303294 1104 80-81 0.22201163150969094 1104 82-83 0.10568875499632568 1104 84-85 0.057292480854581385 1104 86-87 0.16936295935091294 1104 88-89 0.011999809898362912 1104 90-91 0.0024508572460248956 1104 92-93 0.36194787738251577 1104 94-95 0.40241764065086727 1104 96-97 0.372771727950866 1104 98-99 0.4104438830923094 1104 100-101 0.8535441876188123 1105 1 0.11958576852463665 1105 2 0.020126204988152097 1105 3 0.01674003360867715 1105 4 -0.004024271325086204 1105 5 -3.7134365894075927E-4 1105 6 -0.01554526286431468 1105 7 0.06022617276940423 1105 8 0.01569089890866593 1105 9 0.009435461228981978 1105 10-11 0.012013506180629463 1105 12-13 -0.07722640801338088 1105 14-15 -0.00700996026616707 1105 16-17 0.017285229489239384 1105 18-19 0.07129008271554937 1105 20-21 0.002515399067682722 1105 22-23 -0.019274087599967515 1105 24-25 0.03152981222371665 1105 26-27 0.028143547265976565 1105 28-29 0.043262356276436265 1105 30-31 0.011231955594460885 1105 32-33 -0.03693600603645564 1105 34-35 -0.07575589181070796 1105 36-37 0.10839411037175495 1105 38-39 -0.010453398962489757 1105 40-41 0.09029897691569744 1105 42-43 0.012165380905265977 1105 44-45 0.09140583769136157 1105 46-47 0.2371721613947031 1105 48-49 0.1980064655403524 1105 50-51 0.004954432629929784 1105 52-53 -0.10926845370259741 1105 54-55 0.052207879445916205 1105 56-57 0.04184120151654014 1105 58-59 -0.08096963353756337 1105 60-61 0.017327458617536706 1105 62-63 0.06732041000537947 1105 64-65 0.09738413612290486 1105 66-67 0.14259523028425747 1105 68-69 0.2534323622505923 1105 70-71 0.26095291013262667 1105 72-73 0.35019235162264906 1105 74-75 0.38635551223887177 1105 76-77 0.33220887785634545 1105 78-79 0.173506122953615 1105 80-81 0.029314690812157096 1105 82-83 0.07828187080626847 1105 84-85 0.13095346639352812 1105 86-87 0.064989326467213 1105 88-89 0.21021648264110127 1105 90-91 0.36914942329293154 1105 92-93 0.35415151694990143 1105 94-95 0.49759931521642997 1105 96-97 0.3723767832431264 1105 98-99 0.4621246766273721 1105 100-101 0.9498309478569951 1106 1 -0.1468259999932826 1106 2 -0.05939513193104062 1106 3 -0.10282650452884212 1106 4 -0.02820179276461232 1106 5 -0.12045024327659348 1106 6 -0.011962782791734128 1106 7 -0.22786611440144355 1106 8 -0.18602426321088217 1106 9 -0.2666780980564383 1106 10-11 -0.4211115073203331 1106 12-13 -0.28966605871650586 1106 14-15 -0.2543647844296473 1106 16-17 -0.07909003242940571 1106 18-19 -0.3400376288175124 1106 20-21 -0.17542153254185422 1106 22-23 -0.13404339667477672 1106 24-25 -0.2169263528552321 1106 26-27 -0.28597454294204994 1106 28-29 -0.30609898075076813 1106 30-31 -0.22298238296644968 1106 32-33 -0.2123158555817426 1106 34-35 -0.19359329433377326 1106 36-37 -0.40904204358241003 1106 38-39 -0.49175591770182336 1106 40-41 -0.45864369530256255 1106 42-43 -0.533286617777037 1106 44-45 -0.1728251880576792 1106 46-47 -0.2886109618636752 1106 48-49 -0.3042579831883998 1106 50-51 -0.23337466140156238 1106 52-53 -0.1989944921965332 1106 54-55 -0.18273854873977058 1106 56-57 -0.30995098358703643 1106 58-59 -0.3368347886366152 1106 60-61 -0.2899307931699866 1106 62-63 -0.017098063089903803 1106 64-65 -0.16140060185043836 1106 66-67 -0.12064482490767148 1106 68-69 -0.004520716508615408 1106 70-71 0.06448894689844664 1106 72-73 0.07284851724164199 1106 74-75 0.1425932938689236 1106 76-77 -0.01808706321466147 1106 78-79 -0.03140606679429325 1106 80-81 -0.13331350081508475 1106 82-83 -0.09299604918163595 1106 84-85 -0.1427737979876973 1106 86-87 -0.06563213628093933 1106 88-89 0.09765356816158999 1106 90-91 -0.06103575589182597 1106 92-93 0.24493211552852046 1106 94-95 0.2075122610181701 1106 96-97 0.14119172286682868 1106 98-99 0.2622557709612856 1106 100-101 0.841918439486399 1107 1 0.028882527274717518 1107 2 -0.05570715530168968 1107 3 -0.03519234330793353 1107 4 -0.058557276867340136 1107 5 0.037827368898476266 1107 6 -0.0776874152871656 1107 7 -0.050060293987833404 1107 8 0.007711774024578233 1107 9 -0.16979024017922484 1107 10-11 -0.07113315336952297 1107 12-13 0.14939623868391294 1107 14-15 0.03268450125072775 1107 16-17 0.05498120326596023 1107 18-19 0.07607418808312616 1107 20-21 0.07438379472444723 1107 22-23 0.17438446246364947 1107 24-25 0.005986503906022733 1107 26-27 0.07853536003688788 1107 28-29 0.17392288494736619 1107 30-31 0.15024900019130172 1107 32-33 0.19906784065651806 1107 34-35 0.1091424686947775 1107 36-37 0.22246425927947655 1107 38-39 0.05902833531109053 1107 40-41 0.05385007835841549 1107 42-43 0.07495213070578899 1107 44-45 0.1323564722474373 1107 46-47 0.03549876908054017 1107 48-49 0.15073453103387635 1107 50-51 0.27039444362818443 1107 52-53 0.24756421110946292 1107 54-55 0.3693430501344892 1107 56-57 0.3816147803697021 1107 58-59 0.4738359696411436 1107 60-61 0.4211284603211851 1107 62-63 0.3362462337961034 1107 64-65 0.3795819941591816 1107 66-67 0.48025339756553365 1107 68-69 0.690716373270412 1107 70-71 0.7005844956063072 1107 72-73 0.5906379560952679 1107 74-75 0.6236344015231232 1107 76-77 0.6272801508502397 1107 78-79 0.6883537647987339 1107 80-81 0.6516955295907039 1107 82-83 0.702189458522728 1107 84-85 0.6728202850948719 1107 86-87 0.671494132811695 1107 88-89 0.7558241900444358 1107 90-91 0.8459358307458622 1107 92-93 0.7465054396602149 1107 94-95 0.5811429092730585 1107 96-97 0.7322730546196361 1107 98-99 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2316 86-87 -0.9527039041677732 2316 88-89 -0.6827389476179917 2316 90-91 -0.4148568191557196 2316 92-93 -0.49904605990605333 2316 94-95 -0.5525752048533796 2316 96-97 -0.5892288013991056 2316 98-99 -0.332952682749724 2316 100-101 -0.6584344233337696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 17.0 5 31.0 6 61.0 7 176.0 8 458.0 9 854.0 10 1466.0 11 2566.0 12 4251.0 13 5927.0 14 7608.0 15 10915.0 16 15694.0 17 23220.0 18 19761.0 19 10201.0 20 8014.0 21 7722.0 22 7919.0 23 8588.0 24 9562.0 25 10772.0 26 12345.0 27 15148.0 28 18357.0 29 22435.0 30 27512.0 31 34093.0 32 42558.0 33 55625.0 34 76901.0 35 112361.0 36 164003.0 37 243260.0 38 205679.0 39 20066.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63286492801338 19.95587542232813 15.721890702475225 19.68936894718326 2 15.603420186662664 22.3045882999636 39.38147783038852 22.71051368298521 3 19.113563761210415 27.666268976970205 31.10751467707127 22.112652584748115 4 12.39523336149496 17.944704273906776 37.32838749955436 32.3316748650439 5 12.349308685457688 39.76186212180004 32.641023855243475 15.247805337498798 6 29.467065406551352 37.43774525694927 17.30207199371877 15.793117342780604 7 26.6916666735758 36.21018577553222 20.14247185011935 16.955675700772634 8 27.66270386433337 33.18216150291883 19.571307641860393 19.583826990887406 9 28.0950774085445 16.226900350127224 20.31790855568996 35.36011368563832 10-11 24.667106917535044 28.068314332487237 26.024712914564386 21.239865835413333 12-13 26.882753296997368 26.016626874544745 27.58827404452268 19.512345783935203 14-15 23.407410293889647 25.957336379984632 28.117462999519955 22.517790326605773 16-17 20.10408470707625 29.882857031521397 28.331286848133665 21.681771413268685 18-19 20.128418673562535 26.58624712200064 33.46471740678682 19.820616797650008 20-21 21.480010910902863 25.305542589415925 33.706730518110355 19.507715981570854 22-23 21.056990918000004 25.785824319596195 33.687802726424025 19.469382035979777 24-25 20.661424255953676 25.96763435283837 33.992427813214974 19.378513577992983 26-27 20.35649601182627 26.297861297531732 33.785579231400305 19.560063459241697 28-29 19.947914809787903 26.4266425448615 34.073721852481675 19.551720792868924 30-31 20.137655030838275 26.25336717155931 33.89996608991892 19.709011707683494 32-33 19.894613612263324 26.406126024244426 33.627676218128514 20.071584145363737 34-35 20.517803592141163 26.131446532015957 33.60939465216523 19.741355223677655 36-37 20.842767754468206 25.97735904746823 33.22423812299307 19.955635075070493 38-39 20.496124789408753 26.231129697677197 33.30770034357879 19.965045169335262 40-41 20.33557672975399 26.54248185833646 33.05893009267224 20.063011319237315 42-43 20.446407989505648 26.85210061178359 32.46997053024009 20.231520868470675 44-45 20.43853445438348 26.937994297468514 32.10725037330108 20.516220874846926 46-47 20.946985116898382 27.390801843577783 31.258161413376467 20.404051626147364 48-49 21.0179142764521 27.69358768891988 30.49186947044812 20.796628564179905 50-51 21.07462444025659 28.101627266644723 30.143524801991155 20.68022349110753 52-53 21.21329073991011 28.42965908403136 29.56921002907639 20.78784014698213 54-55 20.72615540740532 28.23237569986063 29.654109455590717 21.38735943714333 56-57 21.228587560410002 28.522186193396582 29.225508298006936 21.023717948186476 58-59 21.55255680758258 27.838016205509675 29.352525799393597 21.256901187514146 60-61 21.081174298356814 28.025433347731965 30.038146705214103 20.855245648697117 62-63 21.266021243096738 28.02302896944201 30.152396128785135 20.558553658676114 64-65 21.14390417304898 28.263312695744457 30.009804063815544 20.582979067391022 66-67 21.014349163815268 28.360346661603653 29.77971749384189 20.84558668073919 68-69 21.085817235744315 28.380700967472077 29.66986227886974 20.86361951791386 70-71 20.96427176770721 28.63220722756114 29.438959053437724 20.964561951293927 72-73 20.870583923995113 28.60314741409115 29.282840283782967 21.24342837813077 74-75 20.781870656055343 29.159263530638828 28.945066588842195 21.113799224463637 76-77 20.72093210284438 28.636145433380893 29.454297328735716 21.188625135039004 78-79 20.70298217526591 28.638301082882233 29.253780470312975 21.404936271538876 80-81 20.670847528170654 28.95493938883919 29.132324764059625 21.24188831893053 82-83 20.509076799285648 28.688539201384266 29.21199262596855 21.59039137336154 84-85 20.48566215898581 28.71236972241303 28.89112377515957 21.91084434344159 86-87 20.814371217767857 28.445204633319875 28.74210389733139 21.99832025158088 88-89 20.758365785531115 28.324363896850514 28.493292199119004 22.42397811849937 90-91 20.46337328688324 28.24907101881345 28.72600952467423 22.561546169629082 92-93 20.41818551909204 28.23343005070511 28.753705668268122 22.594678761934727 94-95 20.264183137348866 28.07165544761647 28.76830332973229 22.89585808530237 96-97 20.425566666362666 28.07837112490911 28.517667620403387 22.978394588324836 98-99 20.324674786299983 28.403032840572738 28.245131121852534 23.027161251274737 100-101 20.122736674608923 28.27392119297195 28.76583804347691 22.837504088942215 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2126.0 1 1501.0 2 1039.5 3 1284.0 4 1130.0 5 1080.0 6 1930.5 7 3932.0 8 5992.5 9 6679.0 10 5793.0 11 4708.5 12 4433.5 13 4471.0 14 4585.0 15 4559.5 16 4569.0 17 4665.0 18 4795.0 19 4974.0 20 5152.5 21 5446.5 22 5876.5 23 6632.5 24 7625.5 25 8636.5 26 9792.0 27 11259.0 28 12639.0 29 14120.0 30 16000.0 31 18091.0 32 20448.5 33 22026.0 34 23473.5 35 24651.0 36 26704.0 37 29972.0 38 31967.0 39 32858.5 40 34347.0 41 36315.5 42 37555.5 43 39217.0 44 42333.0 45 45413.0 46 45115.5 47 45356.5 48 50881.0 49 49361.0 50 42210.0 51 37441.5 52 33478.0 53 32461.0 54 31453.5 55 28723.0 56 25790.0 57 23570.5 58 22458.5 59 21052.5 60 17747.0 61 14378.5 62 11578.0 63 8515.0 64 5841.0 65 4398.5 66 3348.5 67 2506.5 68 1845.5 69 1190.0 70 824.5 71 645.0 72 526.0 73 460.0 74 353.5 75 279.5 76 207.5 77 125.0 78 91.0 79 63.5 80 39.5 81 22.5 82 17.0 83 16.0 84 12.0 85 5.0 86 2.5 87 2.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07536482295070278 2 0.0 3 0.0 4 0.0 5 0.00207273990513484 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.0727399051348398E-4 12-13 0.006093855321096429 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.120055582593296E-4 24-25 7.047315677458456E-4 26-27 3.7309318292427123E-4 28-29 0.002653107078572595 30-31 1.658191924107872E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.1454798102696797E-4 40-41 4.14547981026968E-5 42-43 0.012519349027014433 44-45 1.658191924107872E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.14547981026968E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.658191924107872E-4 82-83 7.047315677458456E-4 84-85 5.389123753350584E-4 86-87 0.0 88-89 0.0 90-91 0.008912781592079812 92-93 0.0034407482425238348 94-95 0.0 96-97 0.0 98-99 2.487287886161808E-4 100-101 0.011317159882036227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1206133.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.59571056189458 #Duplication Level Percentage of deduplicated Percentage of total 1 69.2858336569604 29.51279316491459 2 14.771468317340705 12.584023780392812 3 5.859702865306856 7.487946216879454 4 3.145076713832331 5.35867109589426 5 1.744896846324379 3.7162560513197946 6 1.1467321707992055 2.930752298362561 7 0.7911137143812905 2.3588635559531554 8 0.5475295641052287 1.8657928669365296 9 0.40814881793044083 1.5646850023270018 >10 2.067905255891485 15.609034025858056 >50 0.14366698735658612 4.1543025746674545 >100 0.08010952766207032 6.504052103851919 >500 0.004689337265414352 1.2884426884616573 >1k 0.0025400576854327736 1.8886783753801688 >5k 1.9538905272559797E-4 0.7657748967467031 >10k+ 3.9077810545119594E-4 2.4099313020538564 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16096 1.3345128605220156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12860 1.0662174072013617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9201 0.7628511946858265 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3009 0.24947497498202936 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2789 0.23123486381684275 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2640 0.2188813339822391 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1980 0.16416100048667934 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1808 0.14990054993935162 No Hit CTGTGGGGATGAACACTACCTATGTAGTGTCTTGCCCCAAAGAATTTGAA 1532 0.12701750138666298 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1446 0.11988727611299914 No Hit TATTAGGGCATTGTCACCACAATATAGATCTTCCTTGTCCAAGAATGCAA 1318 0.10927484779870876 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1315 0.10902611901009258 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1301 0.10786538466321707 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1299 0.10769956547080628 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.14547981026968E-5 0.0 18-19 0.0 0.0 0.0 1.243643943080904E-4 0.0 20-21 0.0 0.0 0.0 2.07273990513484E-4 0.0 22-23 0.0 0.0 0.0 2.901835867188776E-4 0.0 24-25 0.0 0.0 0.0 6.632767696431489E-4 0.0 26-27 0.0 0.0 0.0 0.0013265535392862978 0.0 28-29 0.0 0.0 0.0 0.004228389406475073 0.0 30-31 0.0 0.0 0.0 0.009907696746544535 0.0 32-33 0.0 0.0 0.0 0.016664828837284113 0.0 34-35 0.0 0.0 0.0 0.024582695274899202 0.0 36-37 0.0 0.0 0.0 0.03440748242523835 0.0 38-39 0.0 0.0 0.0 0.04443954356609097 0.0 40-41 0.0 0.0 0.0 0.05343523475437618 0.0 42-43 0.0 0.0 0.0 0.06446221104969352 0.0 44-45 0.0 0.0 0.0 0.07432845299813537 0.0 46-47 0.0 0.0 0.0 0.0933976601253759 0.0 48-49 0.0 0.0 0.0 0.11806326499648048 0.0 50-51 0.0 0.0 0.0 0.14061467516434756 0.0 52-53 0.0 0.0 0.0 0.17858727022641782 0.0 54-55 0.0 0.0 0.0 0.2395258234373821 0.0 56-57 0.0 0.0 0.0 0.34813739446644776 0.0 58-59 0.0 0.0 0.0 0.46570320188569586 0.0 60-61 0.0 0.0 0.0 0.5857148423930031 0.0 62-63 0.0 0.0 0.0 0.7320502796955228 0.0 64-65 0.0 0.0 0.0 0.9001080312038556 0.0 66-67 0.0 0.0 0.0 1.140545860199497 0.0 68-69 0.0 0.0 0.0 1.4369476666337793 0.0 70-71 0.0 0.0 0.0 1.7164773702402636 0.0 72-73 0.0 0.0 0.0 2.0155322837531187 0.0 74-75 0.0 0.0 0.0 2.331708028882387 0.0 76-77 0.0 0.0 0.0 2.690872399644152 0.0 78-79 0.0 0.0 0.0 3.0267391738722016 0.0 80-81 0.0 0.0 0.0 3.3609477561761434 0.0 82-83 0.0 0.0 0.0 3.6829686278378917 0.0 84-85 0.0 0.0 0.0 4.159242803239776 0.0 86-87 0.0 0.0 0.0 4.7204578599540845 0.0 88-89 0.0 0.0 0.0 5.380542610143326 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9835 0.0 35.94886 1 GTATCAA 14620 0.0 31.041492 1 CGACCGT 70 1.7078492E-8 30.535355 18-19 TATCACG 105 6.908616E-5 27.141413 2 TATCAAC 18145 0.0 24.998669 2 ATCAACG 18225 0.0 24.888935 3 TCAACGC 18430 0.0 24.586319 4 CAACGCA 18665 0.0 24.276768 5 GAGTACT 11230 0.0 24.195843 12-13 AACGCAG 18940 0.0 23.899204 6 CGATCCG 70 1.7812674E-5 23.748735 70-71 GCGACAG 145 2.3331835E-5 22.932667 7 GTACTTT 11995 0.0 22.531105 14-15 AGTACTT 11340 0.0 22.453096 12-13 GTACATG 10330 0.0 22.357464 1 TACATGG 10415 0.0 21.844732 2 ACGCAGA 21065 0.0 21.423313 7 CGCAGAG 21130 0.0 21.289968 8 ACATGGG 10550 0.0 20.664778 3 GCAGAGT 21850 0.0 20.414494 9 >>END_MODULE