FastQCFastQC Report
Thu 26 May 2016
SRR937455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74097
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21202.8611144850668717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12401.6734820573032647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10691.4427034832719274No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8751.180884516242223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.9217647138210723No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6050.81649729408748No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4840.653197835269984No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4560.6154095307502329No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4380.5911170492732499No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4370.5897674669689731No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4130.5573774916663293No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG3640.4912479587567648No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3460.4669554772797819No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC3410.4602075657583977No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA3070.4143217674129857No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2820.38058220980606505No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT2770.3738342982846809No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT2750.3711351336761272No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.34549306989486755No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2290.3090543476793932No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG2250.30365601846228596No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2220.29960727154945543No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA2180.2942089423323481No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.2861114485066872No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC2120.2861114485066872No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1970.2658677139425348No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT1970.2658677139425348No Hit
GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG1910.25777022011687384No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC1870.2523718908997665No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.24697356168265922No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA1770.23887606785699825No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1670.22538024481422997No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1660.22403066250995315No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC1640.2213314979013995No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1520.2051365102500776No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC1450.1956894341201398No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1420.19164068720730934No Hit
GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT1360.18354319338164837No Hit
GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA1330.1794944464688179No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1330.1794944464688179No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG1270.17139695264315694No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG1190.1606002942089423No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG1170.15790112960038868No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT1170.15790112960038868No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA1150.15520196499183503No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.15520196499183503No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1130.15250280038328137No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT1070.14440530655762043No Hit
ACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTA1070.14440530655762043No Hit
GGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTAT1060.1430557242533436No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA1050.14170614194906678No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT1050.14170614194906678No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1040.14035655964478994No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT1040.14035655964478994No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA1030.1390069773405131No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.13630781273195947No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG1010.13630781273195947No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA1000.13495823042768265No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC990.1336086481234058No Hit
GTACATGGGGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGT990.1336086481234058No Hit
GTATCAACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAA980.132259065819129No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG980.132259065819129No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC950.1282103189062985No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA940.12686073660202166No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT920.12416157199346801No Hit
GTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGC870.11741366047208389No Hit
TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG870.11741366047208389No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAAAAAAAA870.11741366047208389No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC850.11471449586353023No Hit
GATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTA840.11336491355925342No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT830.11201533125497658No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC830.11201533125497658No Hit
AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT820.11066574895069975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG800.1079665843421461No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC800.1079665843421461No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC800.1079665843421461No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.1079665843421461No Hit
CCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAA790.10661700203786928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG790.10661700203786928No Hit
GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC780.10526741973359245No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC770.10391783742931564No Hit
TAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAA770.10391783742931564No Hit
CGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATA770.10391783742931564No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC760.1025682551250388No Hit
GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG750.10121867282076198No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTGA200.00206645771.249498
TGGGAGT200.00206645771.249496
GGCTTGA250.00500293456.999598
CATGGGC451.5021751E-552.7774054
TGGGATT500.001606126237.999736
AAGAGCT1054.5580236E-836.1902165
GGAAGAT702.0349878E-433.928339
GGGAAGA702.0349878E-433.928338
GAAGAGC1001.2776363E-633.2497634
AGAGCTG1001.2776363E-633.2497636
GGGGAAG753.0510468E-431.6664417
ATGGGGT1551.3133103E-930.6449455
ATGGGAG650.005809494829.230565
CAGAAGA1153.8009675E-628.9128382
GAGCTGT1153.8009675E-628.9128387
CATGGGT1004.8918908E-528.4997964
TATAGGT1503.248897E-828.4997963
ATGGGAT1205.2933465E-627.7081385
GCTGTCC1205.2933465E-627.7081389
GTGGATT1554.467074E-827.5804528
Produced by FastQC (version 0.11.5)