FastQCFastQC Report
Thu 26 May 2016
SRR937454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72737
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21732.9874754251618847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11951.6429052614212851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11151.5329199719537512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9331.282703438415112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7070.9719949956693292No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5530.7602733134443268No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4780.657162104568514No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4640.6379146789116955No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4440.6104183565448121No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4030.5540508956927012No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3830.5265545733258177No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG3470.4770611930654275No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.44544042234351156No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT3090.42481818056834897No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3020.41519446773993984No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2950.40557075491153055No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT2920.4014463065564981No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA2780.3821988808996797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.336829948994322No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2420.3327055006392895No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2380.3272062361659128No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC2240.3079588105090944No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT2210.3038343621540619No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2210.3038343621540619No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA2200.30245954603571773No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT2100.288711384852276No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1990.27358840755049013No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.24746690130195087No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC1760.24196763682857417No Hit
GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG1760.24196763682857417No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC1630.22409502729009995No Hit
GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT1620.22272021117175578No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA1490.20484760163328153No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1470.2020979693965932No Hit
GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA1380.18972462433149567No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC1370.18834980821315148No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA1370.18834980821315148No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1360.1869749920948073No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG1320.18147572762143063No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT1260.1732268309113656No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG1230.1691023825563331No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1230.1691023825563331No Hit
GTATCAACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAA1220.16772756643798892No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA1220.16772756643798892No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1200.16497793420130058No Hit
ACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTA1150.1581038536095797No Hit
GTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGC1130.15535422137289137No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1100.15122977301785887No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA1090.1498549568995147No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT1070.14710532466282636No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT1050.144355692426138No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG1040.14298087630779382No Hit
GGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTAT1040.14298087630779382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1030.14160606018944968No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG1020.1402312440711055No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1020.1402312440711055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG990.13610679571607298No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT970.13335716347938464No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT960.13198234736104045No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.13060753124269628No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.1292327151243521No Hit
GGTATCAACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAA920.12648308288766377No Hit
GTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATT880.12098381841428708No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC880.12098381841428708No Hit
TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG880.12098381841428708No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAAAAAAAA880.12098381841428708No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA870.11960900229594291No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC860.11823418617759876No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA850.11685937005925456No Hit
AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT850.11685937005925456No Hit
TATCAACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAA820.11273492170422204No Hit
ACCATTGTTAGTTAATCAGACTATAGGCAATAATCACACTATAAATAATC820.11273492170422204No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.11273492170422204No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC810.11136010558587789No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG800.10998528946753372No Hit
CCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAA800.10998528946753372No Hit
GTACATGGGGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGT800.10998528946753372No Hit
GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG790.10861047334918955No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA780.10723565723084537No Hit
CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT780.10723565723084537No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC780.10723565723084537No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA770.1058608411125012No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA760.10448602499415703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC760.10448602499415703No Hit
TAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAA760.10448602499415703No Hit
CGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATA750.10311120887581285No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC730.10036157663912453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTAG301.2935871E-463.3282931
TAGGCAT250.005004003656.995465
CTTTAGG250.005004003656.995462
CATTCCG405.3568423E-447.4962169
ACAGCAG205.374681E-447.49621682-83
GGCATTC550.002564829534.54277
GCATTCC600.003928395531.6641468
CTATTAT600.003928395531.6641461
TAAATCA700.00834410827.1406969
ATGGGTA908.8709727E-426.3867875
ATATAAA908.8709727E-426.3867876
GAAGTTA950.001216098424.9980096
GGGGTGA1151.2663721E-424.7806367
TGGGGTG1551.3814897E-624.5141776
GAAGAGC1000.001639602123.7481084
AATCTAA1601.8212304E-623.7481081
CTTACTT1601.8212304E-623.7481089
GAGTACT9800.023.5057812-13
ATGGGGT1652.3799257E-623.028475
AACTTAC1652.3799257E-623.028477
Produced by FastQC (version 0.11.5)