##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937453_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81675 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.891202938475665 31.0 31.0 34.0 30.0 34.0 2 32.07266605448424 33.0 31.0 34.0 30.0 34.0 3 32.08164064891338 33.0 31.0 34.0 30.0 34.0 4 35.78612794612795 37.0 35.0 37.0 35.0 37.0 5 35.729635751453934 37.0 35.0 37.0 35.0 37.0 6 35.894643403734314 37.0 35.0 37.0 35.0 37.0 7 35.821720232629325 37.0 35.0 37.0 35.0 37.0 8 35.842509947964494 37.0 35.0 37.0 35.0 37.0 9 37.40717477808387 39.0 37.0 39.0 34.0 39.0 10-11 37.358536883991434 39.0 37.0 39.0 34.0 39.0 12-13 37.32906029996939 39.0 37.0 39.0 34.0 39.0 14-15 38.23334557698194 40.0 37.5 41.0 33.0 41.0 16-17 38.30429139883685 40.0 37.5 41.0 33.5 41.0 18-19 38.49038261401898 40.0 38.0 41.0 34.0 41.0 20-21 38.49280073461892 40.0 38.0 41.0 34.0 41.0 22-23 38.367168656259565 40.0 37.5 41.0 34.0 41.0 24-25 38.11679828588919 40.0 37.0 41.0 33.5 41.0 26-27 37.82865625956535 40.0 36.5 41.0 33.0 41.0 28-29 37.68615855524946 39.5 36.0 41.0 33.0 41.0 30-31 37.44711355984083 39.0 35.5 41.0 33.0 41.0 32-33 36.994539332721146 39.0 35.0 41.0 33.0 41.0 34-35 36.61991429445975 39.0 35.0 41.0 33.0 41.0 36-37 36.203501683501685 38.0 35.0 41.0 31.5 41.0 38-39 35.80107131925314 38.0 35.0 40.0 30.5 41.0 40-41 35.42513008876645 38.0 35.0 40.0 30.0 41.0 42-43 34.74688093051729 38.0 35.0 40.0 25.5 41.0 44-45 33.84012243648607 38.0 34.5 40.0 11.0 41.0 46-47 32.71408631772268 38.0 33.0 40.0 2.0 41.0 48-49 31.805668809305175 38.0 33.0 40.0 2.0 41.0 50-51 31.201383532292624 38.0 32.0 40.0 2.0 41.0 52-53 30.85971227425773 38.0 31.0 40.0 2.0 41.0 54-55 30.50077747168656 37.0 30.5 40.0 2.0 41.0 56-57 30.028882767064587 37.0 29.0 40.0 2.0 41.0 58-59 29.641420263238444 36.5 28.0 40.0 2.0 41.0 60-61 29.063709825528008 36.0 26.0 39.5 2.0 41.0 62-63 28.619736761554943 35.5 25.5 39.0 2.0 41.0 64-65 28.144744413835323 35.0 22.0 39.0 2.0 40.0 66-67 27.52221610039792 35.0 18.0 38.5 2.0 40.0 68-69 26.986091215182125 35.0 10.5 37.0 2.0 39.5 70-71 26.444983164983164 34.0 3.5 37.0 2.0 39.0 72-73 25.93224977043159 34.0 2.0 36.5 2.0 39.0 74-75 25.516541169268443 34.0 2.0 36.0 2.0 38.5 76-77 24.36258953168044 31.5 2.0 35.0 2.0 36.5 78-79 24.75540863177227 33.0 2.0 35.0 2.0 37.0 80-81 24.519779614325067 33.0 2.0 35.0 2.0 36.5 82-83 24.21362718089991 33.0 2.0 35.0 2.0 36.0 84-85 23.905766758494032 33.0 2.0 35.0 2.0 36.0 86-87 23.641824303642487 33.0 2.0 35.0 2.0 35.0 88-89 23.244977043158862 32.0 2.0 35.0 2.0 35.0 90-91 22.870958065503523 32.0 2.0 35.0 2.0 35.0 92-93 22.550523415977963 31.5 2.0 34.5 2.0 35.0 94-95 22.213265993265992 31.0 2.0 34.0 2.0 35.0 96-97 21.787566574839303 31.0 2.0 34.0 2.0 35.0 98-99 21.431888582797676 31.0 2.0 34.0 2.0 35.0 100-101 20.03245791245791 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24638285400198612 1101 2 0.5659310808509161 1101 3 0.18576613919488238 1101 4 0.11239488322348734 1101 5 0.12687803460723757 1101 6 0.06472394524666214 1101 7 0.251336409557247 1101 8 0.33269746349540696 1101 9 0.38614119219048604 1101 10-11 0.1937807500147315 1101 12-13 0.24633994765209621 1101 14-15 0.5161820854391337 1101 16-17 0.16273792702758527 1101 18-19 0.17700067991247437 1101 20-21 0.12669153882335848 1101 22-23 0.06280573825269897 1101 24-25 0.3609644041155349 1101 26-27 0.10045512964165937 1101 28-29 0.6424464283574451 1101 30-31 0.8620898515077826 1101 32-33 1.064106742059188 1101 34-35 1.0563856202050133 1101 36-37 0.9220172719300095 1101 38-39 0.7570493122747877 1101 40-41 0.05539215292032651 1101 42-43 0.18719564550012535 1101 44-45 0.606465126124796 1101 46-47 0.9651835736720358 1101 48-49 0.9365362071869541 1101 50-51 0.8507545737160491 1101 52-53 1.1281159385303567 1101 54-55 0.2964085027125485 1101 56-57 0.6904042208847656 1101 58-59 0.8957222653384243 1101 60-61 0.6400381158342725 1101 62-63 0.8638208365351581 1101 64-65 0.9601322284334515 1101 66-67 1.6699274964447355 1101 68-69 1.3878998619747627 1101 70-71 1.3993587771944966 1101 72-73 1.9364572809147518 1101 74-75 2.011563338205974 1101 76-77 2.4296395198973784 1101 78-79 2.4035862063265974 1101 80-81 2.3853123925678545 1101 82-83 2.1564343479600687 1101 84-85 2.1900393301069094 1101 86-87 2.060647981218988 1101 88-89 1.5558908099833388 1101 90-91 1.560001818685322 1101 92-93 2.237485046227718 1101 94-95 2.164627885586089 1101 96-97 2.2839435725744046 1101 98-99 2.2675189680508367 1101 100-101 2.557963035348031 1102 1 0.07929821450355234 1102 2 0.07345459182270275 1102 3 0.26162820816039556 1102 4 0.32493406817646786 1102 5 0.199291827710681 1102 6 0.11550764430622706 1102 7 0.10823296128138082 1102 8 0.13301094311923833 1102 9 0.04382144297418478 1102 10-11 -0.1382725415213173 1102 12-13 -0.13406757585887874 1102 14-15 -0.8418900148743447 1102 16-17 -0.0831084679567411 1102 18-19 0.0771574197243794 1102 20-21 0.17206771437194135 1102 22-23 -0.2610187131579593 1102 24-25 0.2404314887550285 1102 26-27 -0.5402972214555248 1102 28-29 -0.13608021741057996 1102 30-31 -0.2035057597774781 1102 32-33 -0.10078354007247725 1102 34-35 -0.27433538292978454 1102 36-37 -0.2512585901389599 1102 38-39 -0.21465915167505045 1102 40-41 0.19473384571029584 1102 42-43 0.3133711940894628 1102 44-45 1.1211202985385924 1102 46-47 1.6827384326062074 1102 48-49 2.0129468654941647 1102 50-51 1.8409583354715338 1102 52-53 1.9069560639222054 1102 54-55 1.8493865591388783 1102 56-57 1.4008274183769274 1102 58-59 1.737728534930902 1102 60-61 1.7680161722606051 1102 62-63 2.2793380779144705 1102 64-65 2.657624391442859 1102 66-67 2.296338154751947 1102 68-69 2.217758796144043 1102 70-71 1.6129951408308578 1102 72-73 1.7130246790338752 1102 74-75 2.1262185106197684 1102 76-77 1.9177272941920478 1102 78-79 2.3201222564833373 1102 80-81 2.495422110436195 1102 82-83 2.181355978054114 1102 84-85 2.4068888598874736 1102 86-87 2.8663689843537874 1102 88-89 2.5942920639018325 1102 90-91 2.4956601258953555 1102 92-93 1.9254160807104732 1102 94-95 2.198797164582956 1102 96-97 2.3133322873079436 1102 98-99 1.225277588740493 1102 100-101 0.8333548848778136 1103 1 0.1969886321939711 1103 2 0.3604324788005897 1103 3 0.5111777577099446 1103 4 0.18881613205853398 1103 5 -0.14214961373075852 1103 6 0.01657234537092478 1103 7 0.03706179011020794 1103 8 0.36749087759916677 1103 9 0.4367780359307787 1103 10-11 0.3107037074549268 1103 12-13 0.2159733741820773 1103 14-15 0.12813455515022554 1103 16-17 0.2532551684068949 1103 18-19 -0.12992693735997562 1103 20-21 0.022272464576694517 1103 22-23 -0.027439679578932896 1103 24-25 -0.023860075536532577 1103 26-27 -0.13214816330646784 1103 28-29 -0.6724029037332713 1103 30-31 0.15927065299893428 1103 32-33 0.23349164420270796 1103 34-35 0.25473914614474324 1103 36-37 0.9658585269781597 1103 38-39 0.7901729531570538 1103 40-41 0.721515172491614 1103 42-43 1.317670948389221 1103 44-45 0.48643561385390655 1103 46-47 0.29199314186092096 1103 48-49 0.8757642283115246 1103 50-51 0.44476668927988783 1103 52-53 1.0408217477878914 1103 54-55 1.6494684592207811 1103 56-57 1.8619248794743903 1103 58-59 2.1774091246114935 1103 60-61 1.964944919189353 1103 62-63 1.0793380779144677 1103 64-65 1.503856987675455 1103 66-67 1.0393356977494896 1103 68-69 1.3359815643668114 1103 70-71 1.4128722907080125 1103 72-73 1.1340730000821928 1103 74-75 1.5874797718810285 1103 76-77 1.4948771713419227 1103 78-79 1.3954703318314117 1103 80-81 1.6571747721888563 1103 82-83 1.3707089674071042 1103 84-85 1.9351853381839526 1103 86-87 1.217024185008988 1103 88-89 1.4284443980541681 1103 90-91 2.361221141456369 1103 92-93 1.906947612242007 1103 94-95 0.8962992120850046 1103 96-97 0.9546099285855867 1103 98-99 1.1950974085603114 1103 100-101 1.8559593074822374 1104 1 -0.022113965549404924 1104 2 0.199844706562331 1104 3 -0.16458538407261258 1104 4 0.1867169454933233 1104 5 0.3672529927592265 1104 6 -0.0313590812012805 1104 7 0.14998053409691892 1104 8 0.4127108548579841 1104 9 0.6134595718356124 1104 10-11 0.09614934726950963 1104 12-13 0.2350409854826978 1104 14-15 0.21918677241603035 1104 16-17 -0.3244147345057229 1104 18-19 0.2218617445257891 1104 20-21 0.3263483851574662 1104 22-23 -0.027920743166788498 1104 24-25 0.24872804656614989 1104 26-27 0.37104434959478283 1104 28-29 0.3272472318392019 1104 30-31 0.380210038986867 1104 32-33 0.48434443874482724 1104 34-35 0.40924802395458926 1104 36-37 0.7273821865600709 1104 38-39 0.685782154591493 1104 40-41 1.4732863611560063 1104 42-43 1.232391494177726 1104 44-45 0.38471253154829554 1104 46-47 0.7177781501701972 1104 48-49 0.5769362741437689 1104 50-51 0.7520351227619138 1104 52-53 0.7709013419451516 1104 54-55 0.9157590216278493 1104 56-57 1.3739077361174417 1104 58-59 1.4112060283113053 1104 60-61 1.1562332949437497 1104 62-63 0.04050312645815879 1104 64-65 0.8175979130668622 1104 66-67 1.1282887284500944 1104 68-69 0.8841753892596635 1104 70-71 1.0741160587478937 1104 72-73 0.8814271503489657 1104 74-75 0.8601990931440398 1104 76-77 1.1221403568575354 1104 78-79 0.9798751249740683 1104 80-81 1.1975403805156262 1104 82-83 0.966528087498066 1104 84-85 0.7565358148742334 1104 86-87 0.8085137328092138 1104 88-89 1.2046186305390805 1104 90-91 0.7379813968574069 1104 92-93 0.5259015176036819 1104 94-95 1.2040487091548897 1104 96-97 1.5970922166989432 1104 98-99 1.7296465207793297 1104 100-101 2.2113778328036737 1105 1 0.2629345781399124 1105 2 0.061333379701487445 1105 3 0.33496154149373325 1105 4 -0.14415684091444092 1105 5 -0.15404150562265073 1105 6 -0.02631053751195367 1105 7 -0.20510037205195175 1105 8 -0.11971632960803902 1105 9 0.16866992782266976 1105 10-11 0.19142442817565097 1105 12-13 -0.13952212131341923 1105 14-15 -0.3424960754804047 1105 16-17 0.15992183507356117 1105 18-19 0.370793783360746 1105 20-21 0.264794987099215 1105 22-23 0.5729206807814293 1105 24-25 0.2107345190580574 1105 26-27 0.36424823308992416 1105 28-29 0.25210160077124044 1105 30-31 0.3446760584043389 1105 32-33 0.7237619144729805 1105 34-35 1.0529373443429364 1105 36-37 1.6154080765277072 1105 38-39 0.6017044846885824 1105 40-41 1.0874611184375667 1105 42-43 0.32397725469552086 1105 44-45 0.17112029853858957 1105 46-47 0.36334449321226714 1105 48-49 1.4656741382214378 1105 50-51 1.3036856081988049 1105 52-53 1.3193803063464493 1105 54-55 1.01847746822979 1105 56-57 1.5893122668617785 1105 58-59 1.022273989476357 1105 60-61 0.8137737480181819 1105 62-63 1.1660047445811337 1105 64-65 1.1361092399277055 1105 66-67 1.5157320941458856 1105 68-69 2.2004860688713137 1105 70-71 1.1993587771944973 1105 72-73 0.7093883153975078 1105 74-75 0.8662185106197668 1105 76-77 1.2880303244950753 1105 78-79 0.8970919534530317 1105 80-81 1.2099675649816461 1105 82-83 0.8662044629025978 1105 84-85 1.2877979507965662 1105 86-87 0.8357629237477262 1105 88-89 0.771867821477592 1105 90-91 0.13293285316808579 1105 92-93 -0.04458391928952565 1105 94-95 1.0615244373102257 1105 96-97 0.6351504691261276 1105 98-99 0.941944255407158 1105 100-101 0.6091124606353873 1106 1 -0.25389081868548047 1106 2 -0.13549201712390868 1106 3 -0.5463082997761113 1106 4 -0.4508235075811058 1106 5 -0.5578510294321717 1106 6 -0.5329772041786214 1106 7 -1.5854178323694086 1106 8 -0.4905099804016899 1106 9 -0.06815546900272551 1106 10-11 -0.5955596988084793 1106 12-13 -0.6525379943292933 1106 14-15 -0.5739246469089778 1106 16-17 -0.34991943476771326 1106 18-19 -0.0838093912424327 1106 20-21 -1.4710780287737961 1106 22-23 -0.9051745573138064 1106 24-25 -0.6038686555451136 1106 26-27 -0.6008311319894375 1106 28-29 -1.0012317325620899 1106 30-31 -0.7686572749289979 1106 32-33 0.1466190573301276 1106 34-35 -0.6343642429586396 1106 36-37 0.07890014001976908 1106 38-39 -0.2179780549939636 1106 40-41 -0.015078564102118719 1106 42-43 -0.7172925865743167 1106 44-45 -1.4241177966995053 1106 46-47 0.6115984614662366 1106 48-49 0.40472175726905846 1106 50-51 1.554161798674997 1106 52-53 2.48033268729883 1106 54-55 1.4918108015631262 1106 56-57 1.3683598859093955 1106 58-59 1.7168771640795306 1106 60-61 1.9128213670657992 1106 62-63 2.5079095064858983 1106 64-65 2.962458446276912 1106 66-67 1.9357320941458873 1106 68-69 1.8960416244268714 1106 70-71 2.0104698883056074 1106 72-73 1.7430391090483006 1106 74-75 2.1039962883975463 1106 76-77 1.433744610209363 1106 78-79 2.1916951280562067 1106 80-81 2.045205660219743 1106 82-83 1.6122362089343412 1106 84-85 1.7700201730187857 1106 86-87 1.4795724475572491 1106 88-89 1.534407504017274 1106 90-91 1.8269011071363366 1106 92-93 2.319860525154919 1106 94-95 1.7643815801673703 1106 96-97 0.8364203103959689 1106 98-99 1.3355950490579502 1106 100-101 0.818636270159196 1107 1 -0.35706542186008505 1107 2 -0.2643076459395388 1107 3 -0.17298717645498485 1107 4 -0.6495414562990547 1107 5 -0.3160927876739308 1107 6 -0.4816951528965703 1107 7 -0.40715165410323095 1107 8 -0.3589470988388044 1107 9 -0.6456890465363045 1107 10-11 0.0057834025346252815 1107 12-13 -0.2987528905441863 1107 14-15 -0.14993197291629912 1107 16-17 -0.17943713928541172 1107 18-19 0.38887070643766464 1107 20-21 0.23710267940690954 1107 22-23 0.14702324488398943 1107 24-25 -0.49016291683938107 1107 26-27 0.15847900232069634 1107 28-29 -0.5332830146133745 1107 30-31 -0.6564777877495089 1107 32-33 -0.467391931680865 1107 34-35 -0.09513957873397771 1107 36-37 -1.0551047439851118 1107 38-39 -0.47637243838833854 1107 40-41 -0.14984657387012845 1107 42-43 -0.8728176170993436 1107 44-45 -1.3673412399229505 1107 46-47 -1.2572965324287608 1107 48-49 -1.0508643233170218 1107 50-51 -1.5401605456473462 1107 52-53 -1.4839530269868852 1107 54-55 -2.3126763779240562 1107 56-57 -2.6096620921125826 1107 58-59 -2.1115721643697967 1107 60-61 -2.7478929186484855 1107 62-63 -3.2572003836239922 1107 64-65 -3.362480503662038 1107 66-67 -3.048242264828474 1107 68-69 -3.7652831618979157 1107 70-71 -3.8987181458824267 1107 72-73 -4.492919376910184 1107 74-75 -5.087243027841772 1107 76-77 -5.259020957556203 1107 78-79 -4.316241379880299 1107 80-81 -3.7423401273260453 1107 82-83 -3.3485391268409934 1107 84-85 -3.3848943568957424 1107 86-87 -3.508980665995864 1107 88-89 -3.314798845189074 1107 90-91 -3.321297916062683 1107 92-93 -2.759199303904907 1107 94-95 -3.2727063319205385 1107 96-97 -3.860618761643103 1107 98-99 -4.701645488182585 1107 100-101 -3.705631129108202 1108 1 -0.02990492803292355 1108 2 0.34775313278790776 1108 3 0.14681339334558174 1108 4 0.3269542701966728 1108 5 0.5462054079575935 1108 6 0.4546771168090302 1108 7 0.22057864029373064 1108 8 0.33312317656480417 1108 9 0.40274400189674253 1108 10-11 0.6116713417559012 1108 12-13 -0.2424850842763817 1108 14-15 0.5530594800751487 1108 16-17 0.2409094893945536 1108 18-19 0.33770736360765596 1108 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2316 88-89 1.2407567103664832 2316 90-91 1.9776947579299886 2316 92-93 1.3833525886469822 2316 94-95 0.8834291992149907 2316 96-97 1.098325072300728 2316 98-99 -0.299325585862686 2316 100-101 -0.08612563460270906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 7.0 7 23.0 8 57.0 9 107.0 10 234.0 11 425.0 12 640.0 13 925.0 14 1288.0 15 1867.0 16 2569.0 17 3379.0 18 2652.0 19 1382.0 20 1181.0 21 1120.0 22 1077.0 23 1043.0 24 1066.0 25 1098.0 26 1056.0 27 1124.0 28 1258.0 29 1529.0 30 1723.0 31 1995.0 32 2315.0 33 3163.0 34 4309.0 35 6427.0 36 9282.0 37 14189.0 38 10359.0 39 803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.01919425764647 22.895919842232264 15.25496392655471 14.829921973566554 2 16.05019895928987 27.544536271809 36.1505968778696 20.254667891031527 3 23.752678298132842 28.412610958065503 33.24273033363943 14.591980410162227 4 12.254667891031527 21.638200183654728 38.33119069482706 27.775941230486684 5 9.142346270192155 44.7674917026931 32.80099935091179 13.289162676202956 6 22.98989898989899 42.817263544536274 16.607284970921334 17.5855524946434 7 22.16712580348944 42.817263544536274 19.178451178451176 15.83715947352311 8 26.96541169268442 31.33272115090297 18.90174471992654 22.800122436486074 9 25.34312825221916 20.580348943985307 22.416896235078056 31.65962656871748 10-11 24.12549739822467 29.490664217936946 25.813896541169267 20.569941842669117 12-13 28.219412899078637 30.297223667697203 25.64571918332364 15.837644249900517 14-15 22.47566574839302 30.888276706458523 31.243954698500154 15.392102846648301 16-17 16.02020202020202 33.646158555249464 30.40648913376186 19.927150290786656 18-19 15.493112947658402 33.16253443526171 36.07529843893481 15.269054178145087 20-21 18.477502295684115 27.951637588001226 36.69543924089378 16.875420875420875 22-23 17.665352498959276 29.53449078041972 36.38979356955702 16.410363151063986 24-25 19.13682277318641 30.63973063973064 36.32690541781451 13.896541169268442 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33.05234159779614 17.643709825528006 52-53 18.443220079583718 35.000918273645546 29.524334251606977 17.03152739516376 54-55 16.981940618304257 33.078665442301805 29.75329048056321 20.186103458830733 56-57 18.688093051729414 33.700642791551886 29.82124273033364 17.790021426385064 58-59 21.004591368227732 32.26691153963881 28.576063666972757 18.152433425160698 60-61 17.453321089684724 32.36363636363636 32.23018059381696 17.952861952861955 62-63 16.855218855218858 30.98989898989899 32.11998775635139 20.03489439853076 64-65 16.121824303642484 33.94245485154576 30.818487909397003 19.117232935414755 66-67 17.093357820630548 31.749005203550656 29.565962656871747 21.591674318947046 68-69 16.19161310070401 30.899295990205083 30.503826140189776 22.40526476890113 70-71 18.05203550658096 32.083256810529534 29.653504744413834 20.211202938475665 72-73 17.627793082338535 29.37128864401592 32.63054790327517 20.37037037037037 74-75 15.58677685950413 33.902662993572086 29.671870217324763 20.83868992959902 76-77 16.454851545760636 30.07652280379553 30.60238751147842 22.86623813896541 78-79 16.100397918579738 31.898377716559533 29.401285583103764 22.599938781756965 80-81 16.478114478114477 29.06887052341598 30.61952861952862 23.833486378940925 82-83 15.62228344046526 29.094582185491273 31.368839914294462 23.914294459749005 84-85 14.461762617234372 30.389107916840118 30.347479001885546 24.801650464039966 86-87 14.637281910009184 29.92776247321702 29.50596877869605 25.928986838077744 88-89 14.317110498928681 30.08937863483318 28.719314355677994 26.874196510560143 90-91 15.721676309425414 27.646248507668293 29.254599442862826 27.377475740043465 92-93 15.103949849401276 28.51764331366163 29.749369444376423 26.62903739256067 94-95 14.483011937557391 29.004591368227732 29.2500765228038 27.26232017141108 96-97 13.503520048974593 29.278849097030918 29.984695439240895 27.232935414753594 98-99 15.103152739516377 29.951025405570856 27.152739516375878 27.793082338536884 100-101 13.647244653691448 28.570379032307436 27.48121980935122 30.301156504649896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 525.0 1 312.0 2 107.5 3 122.0 4 116.0 5 108.5 6 146.5 7 208.0 8 249.5 9 265.5 10 247.5 11 246.0 12 258.0 13 250.0 14 258.5 15 271.0 16 292.0 17 324.5 18 367.0 19 404.0 20 478.5 21 563.5 22 627.5 23 741.5 24 847.0 25 1047.0 26 1447.0 27 1963.0 28 2107.0 29 2283.5 30 2560.0 31 2721.5 32 2772.0 33 2641.5 34 2954.5 35 3340.0 36 3682.5 37 3684.5 38 2941.5 39 2293.5 40 2118.5 41 1907.5 42 1585.5 43 1446.5 44 1433.0 45 1420.0 46 1752.0 47 2964.0 48 5014.0 49 4720.5 50 2647.5 51 1559.0 52 931.0 53 744.5 54 676.0 55 635.5 56 599.0 57 518.0 58 459.5 59 441.5 60 396.5 61 327.0 62 248.0 63 168.5 64 95.0 65 68.5 66 60.0 67 41.0 68 32.5 69 25.5 70 16.5 71 16.5 72 14.5 73 13.5 74 14.0 75 13.0 76 10.0 77 7.0 78 6.5 79 6.5 80 4.0 81 2.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0440771349862259 2 0.0 3 0.0 4 0.0 5 0.026936026936026935 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003060912151821243 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001224364860728497 24-25 0.0 26-27 0.0018365472910927456 28-29 0.002448729721456994 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009794918885827977 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.001224364860728497 86-87 0.0 88-89 0.0 90-91 0.009182736455463728 92-93 0.001224364860728497 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.00795837159473523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 81675.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.812672176308546 #Duplication Level Percentage of deduplicated Percentage of total 1 85.41247484909456 37.421487603305785 2 6.955622624636709 6.094888276706459 3 2.4088978314330425 3.1662075298438936 4 1.2184216409568522 2.135292317110499 5 0.6595126313436173 1.4447505356596266 6 0.49183992845964675 1.2929292929292928 7 0.3549072211044042 1.088460361187634 8 0.2934272300469483 1.0284664830119374 9 0.2012072434607646 0.7933884297520661 >10 1.5900961323496534 14.267523722069175 >50 0.22635814889336017 6.703397612488522 >100 0.16487815783590432 14.27976737067646 >500 0.0111781801922647 2.9788797061524335 >1k 0.0111781801922647 7.304560759106214 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2406 2.945821854912764 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1307 1.6002448729721457 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 1.490052035506581 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1036 1.268441995714723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 715 0.8754208754208754 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 628 0.7689011325374963 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 583 0.7138047138047138 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 507 0.620752984389348 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 495 0.6060606060606061 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 474 0.5803489439853076 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 453 0.5546372819100092 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 358 0.4383226201408019 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 349 0.4273033363942455 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 346 0.42363024181206 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 334 0.408937863483318 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 321 0.39302112029384756 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 317 0.3881236608509336 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 315 0.3856749311294766 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 285 0.34894398530762166 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 263 0.32200795837159474 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 260 0.31833486378940923 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 251 0.3073155800428528 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 250 0.3060912151821243 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 250 0.3060912151821243 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 243 0.2975206611570248 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 241 0.29507193143556776 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 232 0.28405264768901134 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 232 0.28405264768901134 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 201 0.2460973370064279 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 197 0.24119987756351394 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 194 0.23752678298132845 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 186 0.22773186409550047 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 182 0.2228344046525865 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 179 0.21916131007040096 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 177 0.21671258034894397 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 169 0.206917661463116 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 167 0.204468931741659 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 161 0.19712274257728804 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 158 0.19344964799510256 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 155 0.18977655341291705 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 151 0.18487909397000304 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 145 0.17753290480563208 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 144 0.1763085399449036 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 138 0.1689623507805326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 138 0.1689623507805326 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 137 0.1677379859198041 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 137 0.1677379859198041 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 132 0.1616161616161616 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 128 0.15671870217324763 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 123 0.15059687786960513 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 121 0.14814814814814814 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 119 0.14569941842669115 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 116 0.14202632384450567 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116 0.14202632384450567 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 115 0.14080195898377718 No Hit GTATCAACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAA 114 0.13957759412304868 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 0.13957759412304868 No Hit ACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTA 113 0.1383532292623202 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 113 0.1383532292623202 No Hit GGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTAT 112 0.13712886440159167 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 111 0.13590449954086317 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 109 0.13345576981940618 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 107 0.13100704009794917 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 107 0.13100704009794917 No Hit GTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGC 104 0.12733394551576369 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 103 0.1261095806550352 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 101 0.1236608509335782 No Hit CCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAA 98 0.11998775635139272 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 98 0.11998775635139272 No Hit TAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAA 97 0.11876339149066423 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 95 0.11631466176920723 No Hit GTACATGGGGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGT 95 0.11631466176920723 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAAAAAAAA 94 0.11509029690847873 No Hit GGTATCAACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAA 90 0.11019283746556473 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 89 0.10896847260483623 No Hit AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATT 89 0.10896847260483623 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 88 0.10774410774410774 No Hit TATCAACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAA 87 0.10651974288337925 No Hit GTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATT 86 0.10529537802265075 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 86 0.10529537802265075 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 85 0.10407101316192224 No Hit GATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTA 83 0.10162228344046526 No Hit ACCATTGTTAGTTAATCAGACTATAGGCAATAATCACACTATAAATAATC 82 0.10039791857973676 No Hit CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA 82 0.10039791857973676 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.003060912151821243 0.0 24-25 0.0 0.0 0.0 0.009794918885827977 0.0 26-27 0.0 0.0 0.0 0.017141108050198958 0.0 28-29 0.0 0.0 0.0 0.041016222834404654 0.0 30-31 0.0 0.0 0.0 0.07223752678298133 0.0 32-33 0.0 0.0 0.0 0.10039791857973676 0.0 34-35 0.0 0.0 0.0 0.12794612794612795 0.0 36-37 0.0 0.0 0.0 0.15365779002142638 0.0 38-39 0.0 0.0 0.0 0.19528619528619529 0.0 40-41 0.0 0.0 0.0 0.2895622895622896 0.0 42-43 0.0 0.0 0.0 0.4928068564432201 0.0 44-45 0.0 0.0 0.0 0.6789103152739516 0.0 46-47 0.0 0.0 0.0 1.0700948882767065 0.0 48-49 0.0 0.0 0.0 1.4661769207223752 0.0 50-51 0.0 0.0 0.0 1.7704315886134068 0.0 52-53 0.0 0.0 0.0 2.0532598714416896 0.0 54-55 0.0 0.0 0.0 2.3146617692072238 0.0 56-57 0.0 0.0 0.0 2.9054178145087235 0.0 58-59 0.0 0.0 0.0 3.47842056932966 0.0 60-61 0.0 0.0 0.0 4.116926844199572 0.0 62-63 0.0 0.0 0.0 4.767676767676768 0.0 64-65 0.0 0.0 0.0 5.49433731251913 0.0 66-67 0.0 0.0 0.0 6.4468931741659015 0.0 68-69 0.0 0.0 0.0 7.4998469543924084 0.0 70-71 0.0 0.0 0.0 8.396082032445669 0.0 72-73 0.0 0.0 0.0 9.203550658096113 0.0 74-75 0.0 0.0 0.0 10.02020202020202 0.0 76-77 0.0 0.0 0.0 11.022956841138658 0.0 78-79 0.0 0.0 0.0 12.190388735843282 0.0 80-81 0.0 0.0 0.0 13.562901744719927 0.0 82-83 0.0 0.0 0.0 14.677073767982858 0.0 84-85 0.0 0.0 0.0 16.217324762779306 0.0 86-87 0.0 0.0 0.0 17.591674318947046 0.0 88-89 0.0 0.0 0.0 19.50535659626569 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAGTT 40 5.3433166E-4 47.527576 3 CTAGTTC 40 5.3433166E-4 47.527576 4 TACTAGT 40 5.3433166E-4 47.527576 2 ATGGGTA 50 2.7941867E-5 47.527573 5 CTTTAGG 75 1.3359568E-7 44.35907 2 CCTTTAG 65 2.5495701E-6 43.87161 1 TAGGCAT 65 2.5495701E-6 43.87161 5 AGGCATT 65 2.5603586E-6 43.84474 6 CATGGGT 105 9.968062E-10 40.73792 4 TTAGGCA 70 4.2441116E-6 40.73792 4 CATTCCG 85 3.586556E-7 39.116386 9 GCATTCC 85 3.586556E-7 39.116386 8 GGCATTC 85 3.586556E-7 39.116386 7 TAGTTCT 50 0.001602457 38.022057 5 GTACAAA 55 0.0025583722 34.56551 1 AGTTCTA 55 0.0025660784 34.544342 6 ATGGGAG 100 1.273902E-6 33.2693 5 TTCCAGA 115 1.01128535E-7 33.06266 1 GAAGAGC 120 1.4773468E-7 31.665648 6 TCTATAG 60 0.003930298 31.665648 9 >>END_MODULE