SRR937452_2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR937452_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	98255
Sequences flagged as poor quality	0
Sequence length	101
%GC	36

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2105	2.1423846114701544	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1707	1.7373161671161774	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	1624	1.6528420945498956	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	1253	1.2752531677777212	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	1175	1.1958678947636252	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1085	1.1042695028242837	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	675	0.6869879395450613	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	667	0.6788458602615643	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	643	0.6544196224110732	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	635	0.6462775431275762	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	481	0.4895425169202585	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	476	0.4844537173680729	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	465	0.47325835835326446	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	452	0.4600274795175818	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	440	0.4478143605923363	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.44577884077146196	No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT	432	0.43967228130883923	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	407	0.414228283547911	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	402	0.40913948399572536	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	391	0.397944124980917	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	362	0.3684290875782403	No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA	356	0.3623225281156175	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	356	0.3623225281156175	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	344	0.350109409190372	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT	315	0.3205943717876953	No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA	312	0.3175410920563839	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	293	0.29820365375807845	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	266	0.27072413617627605	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	262	0.2666530965345275	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	257	0.26156429698234185	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	252	0.25647549743015624	No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT	234	0.23815581904228791	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	233	0.2371380591318508	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG	229	0.23306701949010228	No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT	223	0.22696046002747952	No Hit
GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT	220	0.22390718029616816	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	219	0.22288942038573098	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	217	0.22085390056485674	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	215	0.2188183807439825	No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC	210	0.21372958119179686	No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG	205	0.20864078163961125	No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT	199	0.20253422217698844	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	193	0.19642766271436568	No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA	185	0.18828558343086865	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	185	0.18828558343086865	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	171	0.17403694468474887	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA	163	0.16589486540125187	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	155	0.1577527861177548	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	153	0.15571726629688057	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	147	0.14961070683425778	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA	142	0.14452190728207218	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	142	0.14452190728207218	No Hit
CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA	141	0.14350414737163503	No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA	138	0.14045086764032363	No Hit
GCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTA	134	0.13637982799857515	No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT	132	0.13434430817770088	No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC	131	0.13332654826726376	No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT	128	0.13027326853595236	No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC	128	0.13027326853595236	No Hit
ATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATTG	127	0.12925550862551524	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	125	0.12721998880464097	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	123	0.12518446898376673	No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT	123	0.12518446898376673	No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA	120	0.12213118925245535	No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC	118	0.12009566943158109	No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA	117	0.11907790952114396	No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT	116	0.11806014961070682	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	114	0.11602462978983258	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	113	0.11500686987939546	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	110	0.11195359014808408	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	107	0.10890031041677269	No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG	106	0.10788255050633555	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106	0.10788255050633555	No Hit
GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG	105	0.10686479059589843	No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT	105	0.10686479059589843	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	103	0.10482927077502419	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	101	0.10279375095414992	No Hit
GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA	99	0.10075823113327566	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGGA	30	1.3569297E-6	79.177284	3
CATGGGT	75	1.3443787E-7	44.339275	4
ATGGGTA	55	4.9261434E-5	43.18761	5
TGGGTAT	45	9.575694E-4	42.227886	6
ATGGGAT	75	6.851511E-6	38.005096	5
TACATGG	595	0.0	35.929188	2
GTACATG	615	0.0	34.76076	1
GGGCTTG	60	0.0039309305	31.670916	9
ACATGGG	610	0.0	31.151718	3
CATGGGA	230	0.0	28.916922	4
CTCTGTG	70	0.0083494	27.146498	7
ATGGGCT	90	8.882145E-4	26.39243	7
CATGGGG	240	7.2759576E-12	25.732618	4
AGTACAT	400	0.0	23.753185	2
GAGTACT	1765	0.0	23.147581	12-13
GTACTTT	1750	0.0	22.667326	14-15
ATCTCGG	85	3.1233358E-6	22.35594	94-95
AGTACTT	1695	0.0	21.160656	12-13
GATCTCG	95	8.184641E-6	20.002682	94-95
CATGGGC	145	6.0857757E-4	19.657806	4

Summary