##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937452_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98255 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99732329143555 31.0 31.0 34.0 30.0 34.0 2 32.17163503129612 33.0 31.0 34.0 30.0 34.0 3 32.14447101928655 34.0 31.0 34.0 30.0 34.0 4 35.85974250674266 37.0 35.0 37.0 35.0 37.0 5 35.81646735535087 37.0 35.0 37.0 35.0 37.0 6 35.94449137448476 37.0 35.0 37.0 35.0 37.0 7 35.89038725764592 37.0 35.0 37.0 35.0 37.0 8 35.898875375298964 37.0 35.0 37.0 35.0 37.0 9 37.48337489186301 39.0 37.0 39.0 35.0 39.0 10-11 37.426283649687036 39.0 37.0 39.0 34.5 39.0 12-13 37.37091751055925 39.0 37.0 39.0 34.0 39.0 14-15 38.300549590351636 40.0 38.0 41.0 33.5 41.0 16-17 38.35398707444914 40.0 37.5 41.0 33.5 41.0 18-19 38.5356215968653 40.0 38.0 41.0 34.0 41.0 20-21 38.557310060556716 40.0 38.0 41.0 34.0 41.0 22-23 38.41256424609435 40.0 38.0 41.0 34.0 41.0 24-25 38.21631978016386 40.0 37.0 41.0 34.0 41.0 26-27 37.982056892779 40.0 37.0 41.0 33.5 41.0 28-29 37.81599409699252 40.0 37.0 41.0 33.0 41.0 30-31 37.58782250267162 39.0 36.0 41.0 33.0 41.0 32-33 37.14710701745459 39.0 35.0 41.0 33.0 41.0 34-35 36.74179939952165 39.0 35.0 41.0 33.0 41.0 36-37 36.34705104065951 38.5 35.0 41.0 31.5 41.0 38-39 35.97806727393008 38.0 35.0 41.0 31.0 41.0 40-41 35.60287008294743 38.0 35.0 41.0 30.0 41.0 42-43 34.977746679558294 38.0 35.0 40.5 27.0 41.0 44-45 34.125250623377944 38.0 35.0 40.0 15.5 41.0 46-47 33.05326955371228 38.0 33.5 40.0 2.0 41.0 48-49 32.14858276932472 38.0 33.0 40.0 2.0 41.0 50-51 31.562281817719203 38.0 33.0 40.0 2.0 41.0 52-53 31.22466541142944 38.0 31.5 40.0 2.0 41.0 54-55 30.840267670856445 38.0 31.0 40.0 2.0 41.0 56-57 30.39423439010737 37.5 30.5 40.0 2.0 41.0 58-59 30.05518803114345 37.0 29.0 40.0 2.0 41.0 60-61 29.504788560378607 36.0 28.0 40.0 2.0 41.0 62-63 29.083110274286298 36.0 26.5 39.0 2.0 41.0 64-65 28.61686428171594 35.5 25.5 39.0 2.0 40.0 66-67 28.045839906366087 35.0 23.0 38.5 2.0 40.0 68-69 27.471991247264768 35.0 21.5 37.5 2.0 39.5 70-71 26.942394789069258 34.5 19.0 37.0 2.0 39.0 72-73 26.438568011806012 34.0 13.0 36.5 2.0 39.0 74-75 26.02291486438349 34.0 7.5 36.0 2.0 38.5 76-77 24.90691567859142 32.0 2.0 35.0 2.0 36.5 78-79 25.30705816497888 33.5 2.0 35.0 2.0 37.0 80-81 25.042567808254034 33.0 2.0 35.0 2.0 36.5 82-83 24.711098671823315 33.0 2.0 35.0 2.0 36.0 84-85 24.410320085491833 33.0 2.0 35.0 2.0 36.0 86-87 24.118803114345326 33.0 2.0 35.0 2.0 35.0 88-89 23.649351178057096 32.0 2.0 35.0 2.0 35.0 90-91 23.217866775227726 32.0 2.0 35.0 2.0 35.0 92-93 22.944526996081628 32.0 2.0 35.0 2.0 35.0 94-95 22.55930486998117 31.0 2.0 34.0 2.0 35.0 96-97 22.143483792173427 31.0 2.0 34.0 2.0 35.0 98-99 21.543534680168946 31.0 2.0 34.0 2.0 35.0 100-101 19.70462571879294 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.34647219749338376 1101 2 -0.2490531986454485 1101 3 0.06186901774398734 1101 4 -0.15120137505460463 1101 5 -0.2648930151065727 1101 6 -0.10883838288968661 1101 7 -0.390117969206095 1101 8 -0.05976737385632447 1101 9 -0.3373879503215136 1101 10-11 -0.27254266597409327 1101 12-13 -0.050270773833752 1101 14-15 -0.3139381986857117 1101 16-17 0.044325629394272426 1101 18-19 -0.28586414881806377 1101 20-21 -0.06236523530893834 1101 22-23 -0.3668182392912698 1101 24-25 -0.8024452929213339 1101 26-27 0.053922671800847866 1101 28-29 -0.15435340865065683 1101 30-31 0.17207217265371355 1101 32-33 -0.566920141436583 1101 34-35 -0.8947340819998217 1101 36-37 -0.4805870337969722 1101 38-39 -0.5090533840450675 1101 40-41 -0.1257812021355349 1101 42-43 -0.43705511721794466 1101 44-45 -0.858469268784539 1101 46-47 -1.193562922115305 1101 48-49 -1.2037052914165933 1101 50-51 -0.9085371011694505 1101 52-53 -0.012029151614083133 1101 54-55 -0.28377556680148075 1101 56-57 -0.41010777654395625 1101 58-59 0.049540497570809805 1101 60-61 0.10445057455966733 1101 62-63 0.47712691823648967 1101 64-65 0.372566027860568 1101 66-67 0.5025386102154705 1101 68-69 0.2568663647376681 1101 70-71 0.5971035762358028 1101 72-73 0.625750219826056 1101 74-75 0.39992730164803447 1101 76-77 0.4875475073576041 1101 78-79 0.08372823141042929 1101 80-81 -0.2991202686735548 1101 82-83 -0.1513542871456366 1101 84-85 0.30064587633119544 1101 86-87 0.39192056794342633 1101 88-89 0.8687865535657657 1101 90-91 0.17753734964235335 1101 92-93 0.34440448706663673 1101 94-95 0.0564779128736177 1101 96-97 0.3875356117100388 1101 98-99 0.4509958589934513 1101 100-101 0.7793665386407831 1102 1 0.16548226170965563 1102 2 -0.07351029331127279 1102 3 0.05786311430734514 1102 4 0.08046634579717704 1102 5 0.07603045105408768 1102 6 -0.0063715897208069805 1102 7 -0.024231399524460073 1102 8 0.05551831030981447 1102 9 0.04983532608581953 1102 10-11 0.023641183909951735 1102 12-13 0.1077937422952786 1102 14-15 0.3139766231570107 1102 16-17 0.03648249213937049 1102 18-19 0.11959653007715332 1102 20-21 0.23858353129448062 1102 22-23 -0.026189944962787592 1102 24-25 0.19399156139027696 1102 26-27 0.40766502895876044 1102 28-29 0.37712455888044616 1102 30-31 0.9327721515700205 1102 32-33 0.864874081628983 1102 34-35 0.831451876254448 1102 36-37 0.9748209358424944 1102 38-39 1.146122664869118 1102 40-41 1.573839291223102 1102 42-43 2.0118169047091072 1102 44-45 2.1356272945719823 1102 46-47 2.391404398146136 1102 48-49 3.2543170573078406 1102 50-51 2.604176371316317 1102 52-53 3.184534204911319 1102 54-55 3.3189653144972766 1102 56-57 3.0007714138418784 1102 58-59 3.292298245831404 1102 60-61 3.7948785737163178 1102 62-63 3.63729980459534 1102 64-65 3.554771383120954 1102 66-67 3.614029227967947 1102 68-69 4.292708658644479 1102 70-71 4.352469378470676 1102 72-73 4.46243586182479 1102 74-75 4.377873749044198 1102 76-77 4.5898034635035 1102 78-79 4.473186380261367 1102 80-81 4.360314688104857 1102 82-83 4.247338523311882 1102 84-85 4.1853601921650565 1102 86-87 3.645684008803638 1102 88-89 4.090502766932833 1102 90-91 4.152721832037461 1102 92-93 3.5970294080027543 1102 94-95 3.052640679055358 1102 96-97 3.4768672583471876 1102 98-99 3.222617195700174 1102 100-101 3.1525480693175716 1103 1 0.1556783401410229 1103 2 -0.18566121428572302 1103 3 -0.12410361177703066 1103 4 0.08076343432956179 1103 5 0.20749212663341154 1103 6 0.2569973942364143 1103 7 0.1347109653002576 1103 8 0.03754445410066154 1103 9 0.009728374214162727 1103 10-11 -0.14272839422433492 1103 12-13 0.3237028332043721 1103 14-15 0.38921429398290996 1103 16-17 -0.1094622493936015 1103 18-19 -0.008151538847386064 1103 20-21 -0.08494588047022944 1103 22-23 0.15466269912514718 1103 24-25 0.23528686739146565 1103 26-27 0.14793537952323277 1103 28-29 -0.0018118641736251107 1103 30-31 0.19443287646603835 1103 32-33 -0.15599638777089808 1103 34-35 0.4762825357909861 1103 36-37 0.8170669251473086 1103 38-39 0.3964940255346008 1103 40-41 0.4934025710805017 1103 42-43 0.6787806599081563 1103 44-45 0.3469315132291442 1103 46-47 0.7609825324301482 1103 48-49 0.6022820008610204 1103 50-51 0.7249428597298682 1103 52-53 0.8751164984347923 1103 54-55 0.7504864077830717 1103 56-57 0.676573552879308 1103 58-59 0.5997848768474476 1103 60-61 0.7980722754394343 1103 62-63 0.9435980814818485 1103 64-65 1.354310895895761 1103 66-67 1.1894154430600459 1103 68-69 0.768718759654579 1103 70-71 1.3835894022377566 1103 72-73 1.5283895160137355 1103 74-75 1.8938422576597667 1103 76-77 2.051330498559949 1103 78-79 1.9076040867378978 1103 80-81 1.6972873559598796 1103 82-83 1.3031911673998167 1103 84-85 1.074546169586327 1103 86-87 0.9221991603187938 1103 88-89 1.3014356249245118 1103 90-91 0.2804699009620002 1103 92-93 0.7042040960597973 1103 94-95 0.8348747848188758 1103 96-97 0.8226040379074959 1103 98-99 0.6909029948683276 1103 100-101 1.261282472169622 1104 1 -0.1735899525419029 1104 2 -0.1699553754091525 1104 3 0.039052337964285755 1104 4 0.08556757328004494 1104 5 -0.08131053412046896 1104 6 0.15814299660153353 1104 7 -0.28694461489190815 1104 8 -0.106764488988766 1104 9 -0.30549705076735734 1104 10-11 -0.044428320314501946 1104 12-13 0.2256799211570666 1104 14-15 0.3497649222164725 1104 16-17 0.168715873296712 1104 18-19 0.21468659862489403 1104 20-21 -0.30445807559217997 1104 22-23 -0.2704112107048573 1104 24-25 -0.21482399735354818 1104 26-27 -0.5520830979342719 1104 28-29 -0.2107919085195249 1104 30-31 -0.5418014176950834 1104 32-33 -0.66015381570881 1104 34-35 -0.6573094819473724 1104 36-37 -0.7095406136553564 1104 38-39 -0.5751430772000674 1104 40-41 -0.6040660172417489 1104 42-43 -0.8448704856942086 1104 44-45 -1.122303520030279 1104 46-47 -1.0106360928470153 1104 48-49 -0.4416019607058672 1104 50-51 -0.06201205527382925 1104 52-53 -0.038963324181619186 1104 54-55 0.1837827862014052 1104 56-57 0.3039756223545531 1104 58-59 0.07206869059467635 1104 60-61 1.008253354522953 1104 62-63 0.6934133069068302 1104 64-65 0.43792139109162065 1104 66-67 0.7255388724761538 1104 68-69 1.047451206069951 1104 70-71 1.1713758028290364 1104 72-73 1.0379977939146983 1104 74-75 1.298406189662721 1104 76-77 1.6039912524402133 1104 78-79 1.4893668361835743 1104 80-81 1.4756502532252114 1104 82-83 1.0153123795210313 1104 84-85 1.3557730727644497 1104 86-87 1.5074726266898217 1104 88-89 1.3587157431802446 1104 90-91 2.0332415195872784 1104 92-93 2.042988279356173 1104 94-95 1.8622214219216104 1104 96-97 2.417485782179483 1104 98-99 1.9138335196281204 1104 100-101 2.080572937801552 1105 1 0.014435402287915622 1105 2 0.3473382721035918 1105 3 0.20730368149467182 1105 4 -0.05174812191080491 1105 5 0.2900550439420897 1105 6 0.05245193969096107 1105 7 0.15345775523348948 1105 8 0.06155575353569276 1105 9 0.22647208248329775 1105 10-11 0.056680953490094055 1105 12-13 0.05270899653256578 1105 14-15 0.4005779303465502 1105 16-17 0.2841561378688482 1105 18-19 0.022665102138745397 1105 20-21 -0.1951153719956551 1105 22-23 -0.17488275542030607 1105 24-25 -0.22054261386174545 1105 26-27 -0.17685917620352853 1105 28-29 -0.26157046715257337 1105 30-31 -0.1697812991888199 1105 32-33 0.11692348896254146 1105 34-35 0.02237909459941534 1105 36-37 0.15527955997010423 1105 38-39 0.48438565368045516 1105 40-41 0.340539191522204 1105 42-43 0.567455544345755 1105 44-45 0.9533131259666945 1105 46-47 1.3530472473762245 1105 48-49 1.7660596074352348 1105 50-51 1.4960008977370585 1105 52-53 2.145789328429654 1105 54-55 2.0556995562165667 1105 56-57 1.8271256843636472 1105 58-59 1.4023015691946483 1105 60-61 1.2189120289063062 1105 62-63 1.6264434846662361 1105 64-65 1.3363987233225707 1105 66-67 1.202319911472987 1105 68-69 1.1208630842565306 1105 70-71 0.9980603832341615 1105 72-73 1.5896375899736768 1105 74-75 1.6802444148246352 1105 76-77 1.275136353721738 1105 78-79 1.0189934036356902 1105 80-81 1.0719021479475508 1105 82-83 1.3062728314984327 1105 84-85 1.3403396980917215 1105 86-87 1.4324071726454477 1105 88-89 1.471055553019017 1105 90-91 1.7750769887894293 1105 92-93 1.5736827812164478 1105 94-95 1.913585622004291 1105 96-97 2.697732440578271 1105 98-99 2.2228110585560508 1105 100-101 1.9428438486462518 1106 1 -0.3241196396569528 1106 2 -0.2588935375180448 1106 3 -0.010467248140663798 1106 4 0.08581393938006698 1106 5 -0.2697806006393151 1106 6 -0.09906321182418765 1106 7 -0.11276378217448979 1106 8 -0.20235453579832807 1106 9 -0.24784738336159506 1106 10-11 -0.6477788992748401 1106 12-13 -0.8012971667956279 1106 14-15 -0.4150786353100173 1106 16-17 -0.13850265343400991 1106 18-19 0.027201996506150294 1106 20-21 -0.3071681026924509 1106 22-23 0.09026875973120951 1106 24-25 -0.16875353664893566 1106 26-27 -0.01241815583030359 1106 28-29 -0.23666034902210953 1106 30-31 0.11488742192057799 1106 32-33 0.001831895057392785 1106 34-35 0.0747673842758374 1106 36-37 -0.829397721317342 1106 38-39 -1.3396170855765135 1106 40-41 -0.8260418733639412 1106 42-43 -2.9070779259504285 1106 44-45 -2.7957452544476205 1106 46-47 -3.1279063564587375 1106 48-49 -2.8434250698460524 1106 50-51 -2.3028349180479104 1106 52-53 -2.5617521884338963 1106 54-55 -2.832846925550257 1106 56-57 -2.549436548130789 1106 58-59 -2.7486999716374 1106 60-61 -2.554200451833296 1106 62-63 -2.2887251508413833 1106 64-65 -2.0358406192557545 1106 66-67 -2.3143724357278366 1106 68-69 -2.0254731595373414 1106 70-71 -1.6757540321056759 1106 72-73 -1.537267049642825 1106 74-75 -1.761460772643268 1106 76-77 -2.0471543499248988 1106 78-79 -1.583557529423718 1106 80-81 -1.0110459773734561 1106 82-83 -1.0806472164385639 1106 84-85 -1.0517164566762958 1106 86-87 -1.2028008396812062 1106 88-89 -1.0167461932573012 1106 90-91 -1.078115957623858 1106 92-93 -1.360189843334144 1106 94-95 -0.947953498009408 1106 96-97 -0.8781535378500784 1106 98-99 -0.7699555909902571 1106 100-101 -0.9609397500525994 1107 1 -0.15077327276220132 1107 2 0.01546293038681057 1107 3 0.1909012758084998 1107 4 -0.2178680417212675 1107 5 0.2834940816676266 1107 6 -0.20561257643807096 1107 7 0.06579600928852614 1107 8 0.07786703474582168 1107 9 -0.19115139118173374 1107 10-11 -0.011252343393444164 1107 12-13 0.15618083906947078 1107 14-15 -0.059099489008282546 1107 16-17 -0.1459969512508863 1107 18-19 0.07004982967656304 1107 20-21 0.054839065766330464 1107 22-23 0.02673014780550176 1107 24-25 0.13411384686360606 1107 26-27 -0.018120338951845838 1107 28-29 0.44027024726332087 1107 30-31 0.037663570503184474 1107 32-33 0.056735772541912866 1107 34-35 0.3988143050969484 1107 36-37 0.49790758985894 1107 38-39 0.5178283363850298 1107 40-41 0.47959514195049024 1107 42-43 -0.09834543979858523 1107 44-45 0.07701460218320477 1107 46-47 -0.3763586210400405 1107 48-49 -0.4359633559327243 1107 50-51 -0.2117629076210612 1107 52-53 -1.3367603344097851 1107 54-55 -1.7160336313176074 1107 56-57 -1.2402153034256749 1107 58-59 -1.5321799325367174 1107 60-61 -1.9095279200639865 1107 62-63 -1.6067440495054406 1107 64-65 -1.404853326978138 1107 66-67 -1.5157409596770073 1107 68-69 -1.0280798718214719 1107 70-71 -0.9437566388179626 1107 72-73 -1.0473680597438388 1107 74-75 -1.4291049564164808 1107 76-77 -1.6253557184488479 1107 78-79 -1.4410029513852685 1107 80-81 -1.4797654299638765 1107 82-83 -1.6621069753176805 1107 84-85 -1.4821498225935343 1107 86-87 -1.3005525503361461 1107 88-89 -0.9097080700901508 1107 90-91 -0.3246131879920533 1107 92-93 -0.23946648067529708 1107 94-95 -0.5962102591693927 1107 96-97 -1.0296686893652343 1107 98-99 -0.5941654313291309 1107 100-101 0.34925901175906304 1108 1 -0.2723519628892781 1108 2 -0.23490363852814866 1108 3 -0.2112248238982417 1108 4 0.19439979796592866 1108 5 0.3021890963303804 1108 6 0.006997394236414323 1108 7 -0.08498600439671122 1108 8 -0.2654858489296359 1108 9 -0.38042314093735285 1108 10-11 -0.03856172755767062 1108 12-13 -0.07402443952290128 1108 14-15 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-1.3374797301544277 2316 80-81 -0.8469582920091767 2316 82-83 -0.6726308828903171 2316 84-85 -0.6140418381506159 2316 86-87 -1.3188549982885576 2316 88-89 -0.8159080243340249 2316 90-91 -1.0251393833706892 2316 92-93 -1.130338134759029 2316 94-95 -0.2882715723700322 2316 96-97 0.1972816652446987 2316 98-99 -0.38654703736893836 2316 100-101 -1.013701409197239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 5.0 6 9.0 7 25.0 8 63.0 9 125.0 10 276.0 11 458.0 12 772.0 13 1105.0 14 1392.0 15 2118.0 16 2901.0 17 3868.0 18 3043.0 19 1663.0 20 1321.0 21 1189.0 22 1227.0 23 1183.0 24 1100.0 25 1223.0 26 1150.0 27 1331.0 28 1532.0 29 1783.0 30 2082.0 31 2459.0 32 3112.0 33 3841.0 34 5454.0 35 7967.0 36 11681.0 37 17319.0 38 12510.0 39 959.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.74806268647597 22.862845330590716 15.487306905084367 14.901785077848945 2 16.339117602157653 26.943158109002084 35.66637830135871 21.051345987481554 3 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19.31250318049972 32.79985751361254 32.04264414024732 15.844995165640425 48-49 18.445880616762505 32.04875069970994 32.66144216579309 16.843926517734467 50-51 16.00987227113124 32.80647295303038 33.40237138059132 17.781283395247062 52-53 18.68912523535698 34.22726578800061 29.37153325530507 17.712075721337335 54-55 17.1930181670144 31.906264312248737 30.288534934608926 20.612182586127933 56-57 18.915067935474024 33.06091293063966 29.665156989466183 18.358862144420133 58-59 21.429443794208947 31.534781944939187 28.48811765304565 18.547656607806218 60-61 17.899343544857768 31.56277034247621 32.500636099944025 18.037250012721998 62-63 17.216426644954456 30.55976795074042 32.312859396468376 19.910946007836753 64-65 16.40425423642563 33.23545875527963 30.971960714467457 19.388326293827284 66-67 17.57569589333876 31.082387664749884 29.317083100096685 22.024833341814663 68-69 16.643936695333572 30.453412040099742 30.265126456668874 22.637524807897815 70-71 18.663172357640832 31.454887791969877 29.824436415449597 20.057503434939697 72-73 17.934965141723065 29.18121215205333 32.69248384306142 20.19133886316218 74-75 16.02004987023561 33.70464607399114 29.554730039183756 20.720574016589485 76-77 16.99862602412091 30.069716553864943 30.334843010533813 22.59681441148033 78-79 16.570657981782098 31.65945753396774 29.54862347972113 22.22126100452903 80-81 16.928909470255967 29.284514782962702 30.178107984326495 23.608467762454836 82-83 15.905551880311434 29.33845605821587 31.30018828558343 23.455803775889265 84-85 15.032822757111596 30.53432395297949 30.044272556104012 24.388580733804897 86-87 15.08167523281258 30.281919495191083 29.21225382932166 25.424151442674674 88-89 14.972266042440587 30.304819093175922 28.486082133224773 26.23683273115872 90-91 16.01288562733463 27.62165518224104 29.244063552809695 27.121395637614633 92-93 15.726506165112031 28.43482995689765 29.72535609056074 26.113307787429584 94-95 14.94020660526182 28.566994046104522 29.505877563482773 26.98692178515088 96-97 13.850185741183655 29.002595287771616 30.119077909521142 27.028141061523588 98-99 15.68673349956745 29.585771716452093 27.23932624293929 27.48816854104117 100-101 14.182112430913921 27.718403615377568 28.394251223955948 29.705232729752563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 546.0 1 339.5 2 127.5 3 127.0 4 117.5 5 115.0 6 158.5 7 236.0 8 288.5 9 306.0 10 298.0 11 275.0 12 280.0 13 284.0 14 275.0 15 291.0 16 314.0 17 352.0 18 410.5 19 469.0 20 548.5 21 639.0 22 726.0 23 849.0 24 1022.0 25 1250.5 26 1687.0 27 2419.0 28 2634.0 29 2758.5 30 3029.0 31 3166.0 32 3234.5 33 3148.0 34 3502.0 35 3876.0 36 4233.0 37 4279.0 38 3434.0 39 2608.0 40 2350.5 41 2158.5 42 1892.5 43 1741.0 44 1736.0 45 1757.0 46 2049.0 47 3585.5 48 6431.0 49 6192.5 50 3406.5 51 1935.5 52 1236.0 53 974.5 54 844.5 55 792.5 56 740.0 57 681.0 58 641.0 59 582.0 60 505.5 61 424.0 62 326.5 63 224.5 64 150.0 65 109.0 66 76.0 67 56.0 68 38.5 69 34.5 70 29.0 71 25.0 72 25.5 73 21.5 74 20.5 75 17.5 76 14.0 77 11.0 78 9.5 79 8.0 80 7.0 81 5.0 82 3.5 83 2.5 84 2.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052923515342730654 2 0.0 3 0.0 4 0.0 5 0.0030532797313113837 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005088799552185639 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.088799552185639E-4 24-25 0.0 26-27 0.0 28-29 0.0010177599104371278 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011195359014808406 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0061065594626227675 92-93 5.088799552185639E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.008142079283497023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 98255.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.48435194137703 #Duplication Level Percentage of deduplicated Percentage of total 1 85.00826485877872 36.115210421861484 2 7.481493903169394 6.3569284005903 3 2.4459190762523058 3.1173986056689227 4 1.1355197278585631 1.9296727901887947 5 0.7402438732242531 1.5724390616253625 6 0.41683635579618145 1.0625413464963616 7 0.32101190618786385 0.9546587959900259 8 0.2778909038641209 0.9444811968856546 9 0.18925328797642718 0.7236272963207979 >10 1.533191193733081 13.186097399623428 >50 0.2635172364228733 7.78179227520228 >100 0.16050595309393192 13.870032059437179 >500 0.01676927868145557 5.3208488117653046 >1k 0.009582444960831755 7.064271538344105 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2810 2.8599053483283297 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1503 1.529693145387003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1491 1.5174800264617576 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1137 1.1571930181670143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.9821383135718283 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 847 0.8620426441402472 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 815 0.8294743270062592 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 789 0.803012569334894 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 661 0.6727393007989415 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 577 0.5872474683222227 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 574 0.5841941885909114 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 468 0.47631163808457583 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 447 0.45493867996539616 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 427 0.4345834817566536 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 416 0.42338812274184523 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 394 0.4009974047122284 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 392 0.39896188489135415 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 389 0.3959086051600428 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 371 0.3775889267721744 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.3256831713398809 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 315 0.3205943717876953 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 299 0.30431021322070123 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 295 0.3002391735789527 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 293 0.29820365375807845 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 281 0.28599053483283293 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 276 0.28090173528064727 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 272 0.2768306956388988 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 264 0.26868861635540175 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 263 0.26767085644496463 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 253 0.25749325734059336 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 251 0.2554577375197191 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 249 0.2534222176988448 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 246 0.25036893796753346 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 219 0.22288942038573098 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 215 0.2188183807439825 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 206 0.20965854155004837 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 206 0.20965854155004837 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 200 0.20355198208742556 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 195 0.19846318253523995 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 184 0.18726782352043153 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 184 0.18726782352043153 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 173 0.17607246450562314 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 172 0.175054704595186 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 168 0.17098366495343748 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 168 0.17098366495343748 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 165 0.1679303852221261 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 163 0.16589486540125187 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 160 0.16284158566994045 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 155 0.1577527861177548 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 150 0.15266398656556918 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 146 0.14859294692382066 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 146 0.14859294692382066 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 146 0.14859294692382066 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 136 0.1384153478194494 No Hit GTATCAACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAA 134 0.13637982799857515 No Hit ACGCAGAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTA 134 0.13637982799857515 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 130 0.13230878835682663 No Hit GTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGC 129 0.13129102844638948 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 122 0.1241667090733296 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 121 0.12314894916289247 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.12213118925245535 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 120 0.12213118925245535 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 120 0.12213118925245535 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.11907790952114396 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 117 0.11907790952114396 No Hit GGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTAT 115 0.1170423897002697 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 114 0.11602462978983258 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 113 0.11500686987939546 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 112 0.11398910996895832 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 112 0.11398910996895832 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 111 0.1129713500585212 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 111 0.1129713500585212 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 111 0.1129713500585212 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 109 0.11093583023764693 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 107 0.10890031041677269 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 105 0.10686479059589843 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 103 0.10482927077502419 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAAAAAAAA 103 0.10482927077502419 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 100 0.10177599104371278 No Hit ATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATTG 100 0.10177599104371278 No Hit CTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGCTT 99 0.10075823113327566 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 99 0.10075823113327566 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0010177599104371278 0.0 18-19 0.0 0.0 0.0 0.0010177599104371278 0.0 20-21 0.0 0.0 0.0 0.0010177599104371278 0.0 22-23 0.0 0.0 0.0 0.0030532797313113837 0.0 24-25 0.0 0.0 0.0 0.010686479059589842 0.0 26-27 0.0 0.0 0.0 0.014757518701338353 0.0 28-29 0.0 0.0 0.0 0.04020151646226655 0.0 30-31 0.0 0.0 0.0 0.08243855274540736 0.0 32-33 0.0 0.0 0.0 0.1256933489389853 0.0 34-35 0.0 0.0 0.0 0.15571726629688057 0.0 36-37 0.0 0.0 0.0 0.19948094244567705 0.0 38-39 0.0 0.0 0.0 0.2712330161314946 0.0 40-41 0.0 0.0 0.0 0.369955727443896 0.0 42-43 0.0 0.0 0.0 0.5821586687700371 0.0 44-45 0.0 0.0 0.0 0.7969060098722711 0.0 46-47 0.0 0.0 0.0 1.2233474123454278 0.0 48-49 0.0 0.0 0.0 1.6233270571472191 0.0 50-51 0.0 0.0 0.0 1.8874357539056537 0.0 52-53 0.0 0.0 0.0 2.1713907689176124 0.0 54-55 0.0 0.0 0.0 2.437026105541703 0.0 56-57 0.0 0.0 0.0 2.9586280596407306 0.0 58-59 0.0 0.0 0.0 3.450714976337082 0.0 60-61 0.0 0.0 0.0 3.986056689227011 0.0 62-63 0.0 0.0 0.0 4.588061676250573 0.0 64-65 0.0 0.0 0.0 5.217546180855936 0.0 66-67 0.0 0.0 0.0 6.082642104727494 0.0 68-69 0.0 0.0 0.0 7.076993537224569 0.0 70-71 0.0 0.0 0.0 7.9588824996183405 0.0 72-73 0.0 0.0 0.0 8.719658032670093 0.0 74-75 0.0 0.0 0.0 9.523179481960206 0.0 76-77 0.0 0.0 0.0 10.476311638084574 0.0 78-79 0.0 0.0 0.0 11.597374179431071 0.0 80-81 0.0 0.0 0.0 12.753040557732431 0.0 82-83 0.0 0.0 0.0 13.74586535036385 0.0 84-85 0.0 0.0 0.0 15.10610147066307 0.0 86-87 0.0 0.0 0.0 16.333519922650247 0.0 88-89 0.0 0.0 0.0 18.006208335453664 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTA 80 4.1582098E-9 47.499237 5 CCTTTAG 65 2.538567E-6 43.91249 1 AGGCATT 65 2.5654372E-6 43.845448 6 CATGGGT 115 5.2750693E-11 41.303684 4 TTAGGCA 70 4.2704705E-6 40.71363 4 GGGTAAT 50 0.0016087586 37.99939 7 GGCATTC 80 1.0677379E-5 35.624428 7 TAGGCAT 80 1.0677379E-5 35.624428 5 CTTTAGG 80 1.0677379E-5 35.624428 2 GCATTCC 85 1.6173004E-5 33.528873 8 CATTCCG 90 2.3910015E-5 31.666159 9 GTTGCTG 30 0.003943365 31.666155 80-81 GGGTGGC 85 6.3658407E-4 27.94073 8 ATGGGGT 245 0.0 27.14242 5 TACATGG 1030 0.0 25.824825 2 GTACATG 1040 0.0 25.61562 1 CATGGGG 440 0.0 24.829145 4 GGGGTGG 120 1.695539E-4 23.749617 7 TGGGGTG 265 3.092282E-11 23.301512 6 ACATGGG 1060 0.0 22.853407 3 >>END_MODULE