FastQCFastQC Report
Thu 26 May 2016
SRR937451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058740
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76820.72557946237981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55310.5224134348376372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38900.3674178740767327No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17250.16292951999546632No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.12543211742259666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.051.6020361
GTATCAA54500.044.5756531
TCAACGC69550.034.7067264
ATCAACG69650.034.6536143
CAACGCA70850.034.002845
TATCAAC71750.033.6409532
GGACCGA851.6302485E-533.5349046
AACGCAG72600.033.30786
CGACCCG804.4841092E-429.697995
ACGCAGA82350.029.0181127
CGCAGAG82150.028.9730998
GTACATG42700.027.723291
GCAGAGT85150.027.6720479
TACATGG43300.026.7749352
ACATGGG43700.025.441363
TATAACG1151.2789972E-424.7901942
GAGTACT56400.024.04529812-13
ATGGGCG1401.7744456E-523.7583925
AGAGTAC78150.023.37229310-11
GTGGTAT14750.023.206721
Produced by FastQC (version 0.11.5)