##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937451_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1058740 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74761225607798 33.0 31.0 34.0 30.0 34.0 2 31.855197687817594 33.0 31.0 34.0 30.0 34.0 3 31.878622702457637 33.0 31.0 34.0 30.0 34.0 4 35.49755558494059 37.0 35.0 37.0 33.0 37.0 5 35.509512250410864 37.0 35.0 37.0 33.0 37.0 6 35.54937283941289 37.0 35.0 37.0 33.0 37.0 7 35.48971513308272 37.0 35.0 37.0 33.0 37.0 8 35.470514007216124 37.0 35.0 37.0 33.0 37.0 9 37.08736233636209 39.0 37.0 39.0 33.0 39.0 10-11 36.97077800026446 39.0 37.0 39.0 33.0 39.0 12-13 36.829625309329955 39.0 37.0 39.0 33.0 39.0 14-15 37.93746009407408 40.0 38.0 41.0 33.0 41.0 16-17 37.869731945520144 40.0 38.0 41.0 33.0 41.0 18-19 37.829772182027696 40.0 38.0 41.0 33.0 41.0 20-21 37.72237470956043 40.0 38.0 41.0 32.5 41.0 22-23 37.78418308555453 40.0 38.0 41.0 33.0 41.0 24-25 37.749834236923135 40.0 38.0 41.0 33.0 41.0 26-27 37.66215454219166 40.0 37.0 41.0 32.5 41.0 28-29 37.514129531329694 40.0 37.0 41.0 32.0 41.0 30-31 37.231140789995656 39.5 37.0 41.0 32.0 41.0 32-33 36.963762585715095 39.0 36.0 41.0 31.0 41.0 34-35 36.929975253603345 39.0 36.0 41.0 31.0 41.0 36-37 36.788937321722045 39.0 36.0 41.0 30.5 41.0 38-39 36.644777754689535 39.0 36.0 41.0 30.0 41.0 40-41 36.39504599807318 39.0 35.0 41.0 30.0 41.0 42-43 36.10488032944821 39.0 35.0 41.0 30.0 41.0 44-45 35.79261480627916 39.0 35.0 40.5 28.5 41.0 46-47 35.31252007102782 39.0 35.0 40.0 26.5 41.0 48-49 34.91660747681206 39.0 35.0 40.0 24.0 41.0 50-51 33.95118348225249 37.5 33.5 39.5 23.5 40.5 52-53 34.05695543759563 38.0 33.5 39.5 23.0 40.5 54-55 34.35262765173697 38.0 34.0 40.0 23.0 41.0 56-57 34.097420518729805 38.0 34.0 40.0 21.5 41.0 58-59 33.83148223359843 38.0 33.5 40.0 19.5 41.0 60-61 33.499501293990974 38.0 33.0 40.0 18.5 41.0 62-63 33.117042900051004 37.0 33.0 40.0 15.5 41.0 64-65 32.69502191283979 37.0 32.0 39.5 11.5 41.0 66-67 32.2526966016208 36.0 31.5 39.0 8.0 40.5 68-69 31.75271218618358 36.0 31.0 39.0 6.5 40.0 70-71 31.287482762529045 35.0 31.0 38.5 2.0 40.0 72-73 30.783747662315584 35.0 30.0 38.0 2.0 40.0 74-75 30.23005317641725 35.0 29.5 37.0 2.0 39.0 76-77 29.700891153635453 34.0 29.0 36.5 2.0 39.0 78-79 29.140590229895913 34.0 28.5 36.0 2.0 38.5 80-81 28.54019731001001 34.0 27.5 35.5 2.0 37.5 82-83 27.972230670419556 34.0 26.5 35.0 2.0 37.0 84-85 27.380884825358443 33.0 26.0 35.0 2.0 36.5 86-87 26.78020760526664 33.0 25.0 35.0 2.0 36.0 88-89 26.21449364338742 32.0 24.0 35.0 2.0 35.5 90-91 25.63467517993086 32.0 21.5 35.0 2.0 35.0 92-93 24.952960122409657 31.5 19.0 34.0 2.0 35.0 94-95 24.17797476245348 31.0 10.0 34.0 2.0 35.0 96-97 23.31583627708408 31.0 2.0 34.0 2.0 35.0 98-99 22.1257924514045 30.5 2.0 34.0 2.0 35.0 100-101 19.06277981374086 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2908757582235104 1101 2 0.4189867115375634 1101 3 0.28712011102208024 1101 4 0.3662082230982051 1101 5 0.40269183208483383 1101 6 0.32512584574956804 1101 7 0.28730923942881503 1101 8 0.32191163829836 1101 9 0.430586419754448 1101 10-11 0.442620051901244 1101 12-13 0.5247745843037919 1101 14-15 0.7201519451307732 1101 16-17 0.6126437312354795 1101 18-19 0.5027174549214095 1101 20-21 0.6571330069677188 1101 22-23 0.6360040001482687 1101 24-25 0.6512205413173859 1101 26-27 0.5153854662579889 1101 28-29 0.5400206784731267 1101 30-31 0.5752952345357301 1101 32-33 0.5197243946202761 1101 34-35 0.7262268828826066 1101 36-37 0.79932764359949 1101 38-39 0.8550370734024568 1101 40-41 0.8245704041377522 1101 42-43 0.9580915829221865 1101 44-45 1.1420299523125195 1101 46-47 0.981497056154744 1101 48-49 1.2614349703437853 1101 50-51 1.1704961809522771 1101 52-53 1.261895321196036 1101 54-55 1.3274700398722956 1101 56-57 1.5308062391343427 1101 58-59 1.4841369279542178 1101 60-61 1.4346839028758254 1101 62-63 1.4908555989729138 1101 64-65 1.4326187890656357 1101 66-67 1.683600180328579 1101 68-69 1.7279091303361085 1101 70-71 1.5474524847996882 1101 72-73 1.4436606778368528 1101 74-75 1.6210530184386265 1101 76-77 1.627864754716196 1101 78-79 1.6947306411864034 1101 80-81 1.975694514927877 1101 82-83 1.7571377072042118 1101 84-85 1.9712966797800462 1101 86-87 2.109650566356585 1101 88-89 2.28341203962162 1101 90-91 2.297225682289298 1101 92-93 2.3182972623375875 1101 94-95 2.6059101594771548 1101 96-97 2.744046430450858 1101 98-99 3.088946458473359 1101 100-101 2.9484190146542772 1102 1 0.31006892410391274 1102 2 0.28141231398560507 1102 3 0.2197739424641334 1102 4 0.17193817337233952 1102 5 0.3035675174093271 1102 6 0.296727018770099 1102 7 0.14825581599390603 1102 8 0.11727463715084241 1102 9 0.24307653833152898 1102 10-11 0.22301454380485808 1102 12-13 0.2361401332008981 1102 14-15 0.40249237608831123 1102 16-17 0.3337410154379512 1102 18-19 0.4424440901375277 1102 20-21 0.4221741901884286 1102 22-23 0.3966710918223768 1102 24-25 0.5105491145674179 1102 26-27 0.4088060961190152 1102 28-29 0.4610590566447428 1102 30-31 0.466681693798769 1102 32-33 0.6864042365175109 1102 34-35 0.6769929162881922 1102 36-37 0.7697124453335178 1102 38-39 0.7117926516250748 1102 40-41 0.8083488052597758 1102 42-43 1.0313283545656944 1102 44-45 1.087660195841778 1102 46-47 1.332714702463548 1102 48-49 1.222721722861948 1102 50-51 1.2888983229897732 1102 52-53 1.1684897365983034 1102 54-55 1.3736931687770522 1102 56-57 1.3091048493600184 1102 58-59 1.453561382882178 1102 60-61 1.3719069042783545 1102 62-63 1.38874951711648 1102 64-65 1.6524104504197084 1102 66-67 1.609108786729191 1102 68-69 1.77279654586588 1102 70-71 1.8947986533576362 1102 72-73 1.83189706697366 1102 74-75 1.769438075177117 1102 76-77 2.0278456293815026 1102 78-79 1.9806602599547354 1102 80-81 2.1419194288638685 1102 82-83 2.225477628152831 1102 84-85 2.0928366517295522 1102 86-87 2.243645721271797 1102 88-89 2.3370871639362925 1102 90-91 2.457895324008028 1102 92-93 2.5616847416184747 1102 94-95 2.5585706210352335 1102 96-97 2.467251581541003 1102 98-99 2.434953343593847 1102 100-101 2.333521908954925 1103 1 0.3316607483870122 1103 2 0.33771676792093075 1103 3 0.3006970663201436 1103 4 0.3650010165694084 1103 5 0.3762862483428222 1103 6 0.32385392542049374 1103 7 0.3685627104417435 1103 8 0.44743876161697926 1103 9 0.3909380392467199 1103 10-11 0.4111509074412183 1103 12-13 0.34534086535465036 1103 14-15 0.5196244688277929 1103 16-17 0.6191174644922555 1103 18-19 0.32497276615949033 1103 20-21 0.2880137264910516 1103 22-23 0.21437182397612986 1103 24-25 0.36393715605613863 1103 26-27 0.23327742009705332 1103 28-29 0.09025094194065275 1103 30-31 0.32555173650774094 1103 32-33 0.3190933762368573 1103 34-35 0.31649322135225333 1103 36-37 0.44181037089788333 1103 38-39 0.5131764222169011 1103 40-41 0.42801659049467133 1103 42-43 0.22154617030699342 1103 44-45 0.32611718180883287 1103 46-47 0.3291378263195597 1103 48-49 0.9406939620321779 1103 50-51 0.8814431674070917 1103 52-53 0.775774666433577 1103 54-55 0.9181446635909651 1103 56-57 0.9830627505192595 1103 58-59 1.1913158063111027 1103 60-61 0.8910679781038553 1103 62-63 1.0476125433519883 1103 64-65 0.8802182600597277 1103 66-67 1.196618243715161 1103 68-69 1.2319890412899213 1103 70-71 1.341761435541894 1103 72-73 1.248874492232968 1103 74-75 1.3731403326511895 1103 76-77 1.0715308032680184 1103 78-79 0.9778411629443617 1103 80-81 0.7979468846295816 1103 82-83 0.8545990436500865 1103 84-85 0.955192356427542 1103 86-87 1.1815258310948593 1103 88-89 1.1102555592993184 1103 90-91 1.1708843417017398 1103 92-93 1.4440526488790013 1103 94-95 1.3836359047448106 1103 96-97 1.5062973411505212 1103 98-99 1.629226680994698 1103 100-101 1.4228211768011718 1104 1 0.02914082879828328 1104 2 0.1184952809406461 1104 3 0.022237706469240948 1104 4 0.10600709976575473 1104 5 -0.004952692489979427 1104 6 -0.012275392235430616 1104 7 0.011888208562339742 1104 8 0.052982480010008715 1104 9 -0.008876376148691634 1104 10-11 0.14584279511672804 1104 12-13 0.15254489848241803 1104 14-15 0.24251903897300764 1104 16-17 0.27541425327655844 1104 18-19 0.17060576933196359 1104 20-21 -0.056080809102454054 1104 22-23 0.09363123931918693 1104 24-25 0.0270435125251538 1104 26-27 0.17177078197917695 1104 28-29 -0.029102338901985547 1104 30-31 0.10982337582968427 1104 32-33 0.16443713965181672 1104 34-35 0.2554348394196708 1104 36-37 0.41646524635464743 1104 38-39 0.35188795725547806 1104 40-41 0.439022752217646 1104 42-43 0.40998783549037654 1104 44-45 0.5078919360204779 1104 46-47 0.547655845563817 1104 48-49 0.6334146742163682 1104 50-51 0.5150653914906869 1104 52-53 0.4445938353077139 1104 54-55 0.5622698245705564 1104 56-57 0.6257731233637074 1104 58-59 0.7872485194585508 1104 60-61 0.7585215688649285 1104 62-63 0.76148047726398 1104 64-65 0.8799551958458878 1104 66-67 1.1476071557548586 1104 68-69 0.9322116642888005 1104 70-71 0.8497763870124366 1104 72-73 0.849098031375874 1104 74-75 0.8003644685964915 1104 76-77 0.7596779935851643 1104 78-79 0.9368548422834806 1104 80-81 0.9787845543779845 1104 82-83 1.1130354213736489 1104 84-85 1.048755528484623 1104 86-87 1.076553393942337 1104 88-89 1.063400027359922 1104 90-91 1.3914119883109635 1104 92-93 1.2789778915262922 1104 94-95 1.1186242304523368 1104 96-97 1.3245291308247893 1104 98-99 1.5121801199376286 1104 100-101 1.190402635093065 1105 1 0.09439065877536734 1105 2 -0.09618112239268584 1105 3 0.11061505300548546 1105 4 0.10777584094248027 1105 5 0.08644232511572625 1105 6 0.08111087343276324 1105 7 0.18134291584006945 1105 8 0.2965124951832223 1105 9 0.12634369824655067 1105 10-11 0.12604816771519012 1105 12-13 0.1972653254944987 1105 14-15 0.09836286176702203 1105 16-17 0.17465289002589657 1105 18-19 0.2565286260689632 1105 20-21 0.24026253536460018 1105 22-23 0.1683812962030018 1105 24-25 0.24278499472271875 1105 26-27 0.3026280871819367 1105 28-29 0.33609180149859696 1105 30-31 0.2997336313857559 1105 32-33 0.41483878343715475 1105 34-35 0.48592588823164107 1105 36-37 0.563530041114042 1105 38-39 0.33976950313778786 1105 40-41 0.3999781364442967 1105 42-43 0.5571673407205466 1105 44-45 0.5960628037093372 1105 46-47 0.6586468686052243 1105 48-49 0.9310138626319286 1105 50-51 0.6266833936805583 1105 52-53 0.364120825895796 1105 54-55 0.8005706136528872 1105 56-57 0.9086673722521184 1105 58-59 0.9582224333546705 1105 60-61 0.9084258406200121 1105 62-63 0.8663318187793649 1105 64-65 0.957037803727161 1105 66-67 0.8343048895787994 1105 68-69 0.8264655811013952 1105 70-71 0.8432162051853389 1105 72-73 1.0541286983559814 1105 74-75 1.0874376356488789 1105 76-77 0.9853505740742001 1105 78-79 1.3507377634343314 1105 80-81 1.4327652277064438 1105 82-83 1.5381961103153081 1105 84-85 1.424540116677175 1105 86-87 1.6791472962479865 1105 88-89 1.478646903149535 1105 90-91 1.4183067224403771 1105 92-93 1.548483071411166 1105 94-95 1.446013885264957 1105 96-97 1.6014955197140281 1105 98-99 1.8129751734964188 1105 100-101 1.4664950244775987 1106 1 0.07400094189791417 1106 2 -0.40874121629841653 1106 3 0.03273436222003667 1106 4 -0.030064792294368203 1106 5 0.047631393307582925 1106 6 0.049596643499192794 1106 7 0.22317585705698662 1106 8 0.10363165779644845 1106 9 0.15013254380660612 1106 10-11 -0.4013490925587817 1106 12-13 -0.19616966490563925 1106 14-15 -0.07718413811266345 1106 16-17 0.07681116486193673 1106 18-19 0.30576746187244197 1106 20-21 0.18242376244803182 1106 22-23 0.14894347690279375 1106 24-25 0.20259702149110836 1106 26-27 0.026165283982599874 1106 28-29 0.2959083779633289 1106 30-31 0.13187662935255418 1106 32-33 0.015264736118290045 1106 34-35 0.02054806628249395 1106 36-37 0.1566370489607536 1106 38-39 0.6498677058055264 1106 40-41 0.7544015028758295 1106 42-43 0.6715674329277235 1106 44-45 0.5951619638353876 1106 46-47 0.7005258123072835 1106 48-49 0.5309722076343917 1106 50-51 0.23603736713257462 1106 52-53 0.43420434821003084 1106 54-55 0.8057643447779625 1106 56-57 0.3716434600592322 1106 58-59 0.29612736896635994 1106 60-61 0.3605392756710728 1106 62-63 0.4560806452104771 1106 64-65 0.31986294272038407 1106 66-67 0.6542193148459923 1106 68-69 0.41931325625711935 1106 70-71 0.44550197262305957 1106 72-73 0.4957419397921896 1106 74-75 0.5010571010696729 1106 76-77 0.828210634514388 1106 78-79 0.8842619481034717 1106 80-81 1.0090247100318877 1106 82-83 0.8469718679155775 1106 84-85 0.5178655099478711 1106 86-87 0.595641158707636 1106 88-89 1.166189935694021 1106 90-91 0.7911897234605192 1106 92-93 1.0171002771494422 1106 94-95 0.734559670881243 1106 96-97 0.3952643567206522 1106 98-99 0.7104856807673414 1106 100-101 0.1741027233726271 1107 1 0.10124875984449844 1107 2 0.15033480997351845 1107 3 0.06379320046470127 1107 4 -0.013735384637669767 1107 5 -0.007729376766071994 1107 6 -0.00835822620300064 1107 7 0.06830641544637928 1107 8 0.06601135007446857 1107 9 0.03526383182722981 1107 10-11 0.129284344616714 1107 12-13 0.1367122091000752 1107 14-15 0.23279578399920098 1107 16-17 0.3894555439001479 1107 18-19 0.2577205934887985 1107 20-21 0.16653669372849578 1107 22-23 0.23893371808500063 1107 24-25 0.1730182923263186 1107 26-27 0.4135877443773026 1107 28-29 0.3046157450238596 1107 30-31 0.0806584709157292 1107 32-33 0.22118947205041906 1107 34-35 0.5447255699273938 1107 36-37 0.49573642343225544 1107 38-39 0.614673421945092 1107 40-41 0.4732667699534332 1107 42-43 0.2551180269908784 1107 44-45 0.4480559288730248 1107 46-47 0.5314213955627665 1107 48-49 0.6326743330762383 1107 50-51 0.4707988233201732 1107 52-53 0.41441440383138684 1107 54-55 0.5826020708822099 1107 56-57 0.7093506720731284 1107 58-59 0.8624449852611136 1107 60-61 0.7662578907788671 1107 62-63 0.802142134882935 1107 64-65 0.6562737563764713 1107 66-67 0.6922519945079166 1107 68-69 0.9125537440156037 1107 70-71 0.8699486580777354 1107 72-73 0.9645612791892759 1107 74-75 1.1178489125225362 1107 76-77 1.2808145711354904 1107 78-79 1.046194480666525 1107 80-81 1.3597564910747657 1107 82-83 1.2829740880791967 1107 84-85 1.0752790193308002 1107 86-87 0.9324096219609288 1107 88-89 0.9627622323777167 1107 90-91 0.9097378615328076 1107 92-93 0.8813875642371833 1107 94-95 0.5945173783277831 1107 96-97 0.6173178517194202 1107 98-99 0.854642572329805 1107 100-101 0.5777764166486854 1108 1 0.08859225628503964 1108 2 0.04743236237827375 1108 3 0.025959908589264558 1108 4 0.041851757886682606 1108 5 0.05905732038202416 1108 6 0.0861953907023505 1108 7 0.15126169224693342 1108 8 0.008138100683510174 1108 9 0.02241978064054706 1108 10-11 0.35055204812562835 1108 12-13 0.002216870152160766 1108 14-15 0.0043084839348424 1108 16-17 0.05836389825855548 1108 18-19 0.13381083745864686 1108 20-21 0.12386171179229422 1108 22-23 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-1.2098402495411236 2316 60-61 -1.132860897579235 2316 62-63 -0.9167590671784787 2316 64-65 -0.7673885926424902 2316 66-67 -0.675958480369033 2316 68-69 -0.6915271242720316 2316 70-71 -0.6789857541852875 2316 72-73 -0.5789664286638256 2316 74-75 -0.28045328423745985 2316 76-77 -0.2624864843252226 2316 78-79 -0.27783002994113204 2316 80-81 -0.6093167096106562 2316 82-83 -0.4357213586189097 2316 84-85 -0.25056272907540134 2316 86-87 0.22536426897795891 2316 88-89 0.5310770316537123 2316 90-91 0.34083312305109104 2316 92-93 0.5017359659359535 2316 94-95 0.23180844822352142 2316 96-97 0.37151333217426696 2316 98-99 0.8534667125778057 2316 100-101 1.782719207012903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4805.0 3 897.0 4 929.0 5 891.0 6 1041.0 7 1224.0 8 1558.0 9 1949.0 10 2488.0 11 3280.0 12 4475.0 13 5715.0 14 6554.0 15 8590.0 16 11228.0 17 14751.0 18 12882.0 19 8446.0 20 8274.0 21 8736.0 22 9430.0 23 10549.0 24 11978.0 25 12961.0 26 15073.0 27 17644.0 28 20584.0 29 24379.0 30 29299.0 31 35590.0 32 44935.0 33 57929.0 34 77086.0 35 104702.0 36 144940.0 37 187487.0 38 132114.0 39 13344.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.796204668931836 19.449934716818802 15.389207145713197 22.36465346853616 2 14.649950563874883 20.176189592275083 41.621239729218836 23.5526201146312 3 18.147002615644244 25.44514249673755 31.105142837661486 25.30271204995672 4 11.746127650225201 16.921666597218803 38.90721891608299 32.42498683647301 5 12.591898098823307 37.23181097142316 35.517105855055995 14.65918507469754 6 29.768152986840864 37.25456783110859 18.48442677269715 14.492852409353404 7 26.774134304253078 33.38511698181797 22.01164800751289 17.82910070641606 8 25.563940382836638 34.445356456143436 20.87661049747556 19.11409266354437 9 25.928226376077728 16.413610136400564 21.47073248787582 36.18743099964588 10-11 23.713826480732312 26.983758755619103 28.53042895727944 20.77198580636914 12-13 24.844382355711943 24.650194637446038 29.10017993844884 21.40524306839318 14-15 21.960938010320728 25.4947596421921 28.044032069796966 24.50027027769021 16-17 20.577915503677467 28.966489344833775 28.573822223463374 21.881772928025388 18-19 20.29669068493475 26.816976064553533 32.19053703900898 20.69579621150274 20-21 20.96659152319122 25.922227212855454 32.451758220069884 20.659423043883443 22-23 20.762301956159167 25.826488132987773 32.34837542050891 21.06283449034415 24-25 20.5978250323377 26.085413177121612 32.377030365017255 20.939731425523426 26-27 20.36592511119546 26.372751598952366 32.20475109160385 21.056572198248322 28-29 20.15651204963852 26.224478155104276 32.599087454847286 21.01992234040992 30-31 20.30882284016458 26.154120738784982 32.46228362825176 21.07477279279868 32-33 20.120969803467858 26.274078927187105 32.21700337242264 21.387947896922405 34-35 20.587366507611907 26.34704233886238 32.052989850791484 21.012601302734225 36-37 20.53065327774812 26.127370796557354 32.00968197531986 21.33229395037467 38-39 20.228008404793005 26.49847615802525 32.18467828951104 21.0888371476707 40-41 20.496385902511648 26.32820567181894 32.170199998674946 21.005208426994475 42-43 20.2845184640717 26.39257338369857 31.84667221687658 21.47623593535315 44-45 20.44281026108966 26.63515883751066 31.530530821415354 21.391500079984326 46-47 20.5621349388922 26.78343888746888 31.061079397536755 21.593346776102166 48-49 20.67288142894404 27.028630099829726 30.715404566255007 21.583083904971225 50-51 20.716520342693524 27.061634081491974 30.533877345412954 21.68796823040155 52-53 20.92116203792268 27.287018745418766 30.2125219473113 21.579297269347254 54-55 20.688545185925754 27.157946359805024 30.445262754777197 21.70824569949202 56-57 20.64885656941958 27.564667727681652 30.177026706627462 21.609448996271304 58-59 20.834474884224264 27.082602859058568 30.433817729456457 21.64910452726071 60-61 20.837371290127198 27.045682540884314 30.689212976983647 21.427733192004844 62-63 20.73008624463189 26.96411797413844 30.8037431826517 21.50205259857797 64-65 20.629820811663684 26.87077421152921 30.631888605980134 21.86751637082697 66-67 20.39613312139858 27.215034433087943 30.396516421078836 21.992316024434643 68-69 20.480134620544494 26.95146079474768 30.71755192009839 21.850852664609437 70-71 20.532455781694832 27.02952745472167 30.52572649766794 21.91229026591556 72-73 20.654212374128498 26.926882730990968 30.332623971508166 22.08628092337236 74-75 20.47540826115084 27.02893026042488 30.264731451785025 22.230930026639257 76-77 20.531705530044217 26.93382103062023 30.371367474036653 22.1631059652989 78-79 20.513138476771605 26.86380455906422 30.24026861012059 22.382788354043587 80-81 20.44994827147207 27.14129129171766 30.1649255024009 22.243834934409364 82-83 20.5747733474166 27.013514447179706 30.189489983181474 22.22222222222222 84-85 20.458420020885452 26.727367274003953 30.061941873719206 22.752270831391392 86-87 20.413324816050345 26.68994724016372 30.396621477748596 22.500106466037334 88-89 20.56213332778065 26.4140291033097 30.27997347963388 22.743864089275768 90-91 20.374895888543954 26.30645680321042 30.79432308624214 22.524324222003482 92-93 20.445178299900473 26.08515405048932 30.70915330085881 22.760514348751396 94-95 20.112087421184835 26.12589036765583 30.57488931331666 23.187132897842673 96-97 20.047591620732266 26.10889754124158 30.727546963360226 23.115963874665933 98-99 20.03848786061071 26.514359114646684 30.40394268659655 23.04321033814605 100-101 20.175292900126486 26.153204783313765 30.711037859461882 22.960464457097864 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1733.0 1 1008.5 2 329.0 3 370.5 4 286.5 5 243.0 6 466.0 7 1131.0 8 2076.5 9 2428.0 10 2068.0 11 1813.5 12 1859.5 13 1941.5 14 2068.0 15 2285.5 16 2399.0 17 2429.5 18 2515.5 19 2647.5 20 2882.0 21 3250.5 22 3755.5 23 4424.0 24 5215.0 25 6269.0 26 7921.5 27 9671.5 28 10824.0 29 12486.5 30 14959.5 31 17200.5 32 19585.0 33 22689.0 34 26276.0 35 28808.5 36 31618.5 37 34789.0 38 36169.5 39 36174.5 40 35850.5 41 36521.0 42 37296.5 43 38197.5 44 39003.0 45 39329.0 46 38513.5 47 37649.5 48 38945.5 49 38919.0 50 36786.5 51 34504.0 52 31867.5 53 29959.5 54 28374.5 55 25459.5 56 22283.5 57 19251.5 58 16604.0 59 14468.0 60 12374.5 61 10084.0 62 7784.5 63 5868.0 64 4325.0 65 3278.5 66 2420.5 67 1811.5 68 1338.5 69 908.0 70 635.5 71 481.0 72 360.0 73 276.0 74 231.0 75 195.5 76 127.5 77 65.0 78 41.5 79 32.5 80 25.0 81 19.5 82 16.0 83 12.5 84 9.5 85 6.0 86 4.0 87 3.0 88 3.5 89 3.5 90 3.0 91 3.0 92 3.0 93 1.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3152804276781835 2 0.2674877684795134 3 0.26314298127963426 4 0.26370969265353156 5 0.2658820862534711 6 0.23046262538489148 7 0.22876249126319967 8 0.2330128265674292 9 0.24425260214972513 10-11 0.248644615297429 12-13 0.24042730037591853 14-15 0.22847913557625102 16-17 0.22427602621984624 18-19 0.21275289495060165 20-21 0.2187978162721726 22-23 0.21553922587226326 24-25 0.2182311048982753 26-27 0.21412244743752007 28-29 0.23268224493265577 30-31 0.24562215463664358 32-33 0.2385854884107524 34-35 0.23612973912386423 36-37 0.22139524340253508 38-39 0.2077941704290005 40-41 0.2061884882029582 42-43 0.21945897954171942 44-45 0.2156336777679128 46-47 0.2276290685154051 48-49 0.2353268980108431 50-51 0.24897519693220244 52-53 0.264654211610027 54-55 0.2359880612803899 56-57 0.20821920395942348 58-59 0.19490148667283752 60-61 0.19834898086404595 62-63 0.20330770538564708 64-65 0.1944292271945898 66-67 0.20075750420310937 68-69 0.20217428263785256 70-71 0.20439390218561687 72-73 0.20368551296824527 74-75 0.209352626707218 76-77 0.22167859908948373 78-79 0.22092298392428736 80-81 0.21374463985492187 82-83 0.20467725787256552 84-85 0.1911234108468557 86-87 0.19504316451631185 88-89 0.20718023310727848 90-91 0.20590513251600961 92-93 0.20963598239416664 94-95 0.2034493832291214 96-97 0.21265844305495213 98-99 0.21752271568090373 100-101 0.19915182197706707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1058740.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.724799631149715 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63825106125199 47.94189935809519 2 10.703075775318748 12.570719606852116 3 3.0952125164240125 5.452972045284805 4 1.3546179120479422 3.1819866184712717 5 0.7458645991491673 2.1900374568501317 6 0.4587838412702485 1.6165193491562722 7 0.31615875224150347 1.2996451563911617 8 0.2344783801778313 1.1015756715023752 9 0.17862382840059085 0.9440683678956211 >10 1.120239918810306 12.683311751587292 >50 0.10739338956397175 4.294700191983996 >100 0.04584712388994957 4.5627967350918395 >500 4.843004845772134E-4 0.1523460047044379 >1k 6.457339794362846E-4 0.7548095142854602 >5k 3.228669897181423E-4 1.252612171848001 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7682 0.72557946237981 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5531 0.5224134348376372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3890 0.3674178740767327 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1725 0.16292951999546632 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1328 0.12543211742259666 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.445189564954568E-5 0.0 4 0.0 0.0 0.0 9.445189564954568E-5 0.0 5 0.0 0.0 0.0 9.445189564954568E-5 0.0 6 0.0 0.0 0.0 1.8890379129909136E-4 0.0 7 0.0 0.0 0.0 1.8890379129909136E-4 0.0 8 0.0 0.0 0.0 1.8890379129909136E-4 0.0 9 0.0 0.0 0.0 1.8890379129909136E-4 0.0 10-11 0.0 0.0 0.0 2.833556869486371E-4 0.0 12-13 0.0 0.0 0.0 3.3058163477340987E-4 0.0 14-15 0.0 0.0 0.0 3.778075825981827E-4 0.0 16-17 0.0 0.0 0.0 5.667113738972742E-4 0.0 18-19 0.0 0.0 0.0 7.556151651963654E-4 0.0 20-21 0.0 0.0 0.0 9.917449043202297E-4 0.0 22-23 0.0 0.0 0.0 0.0010861967999697755 0.0 24-25 0.0 0.0 0.0 0.0013695524869184125 0.0 26-27 0.0 0.0 0.0 0.0022668454955890966 9.445189564954568E-5 28-29 0.0 0.0 0.0 0.00415588340858001 9.445189564954568E-5 30-31 0.0 0.0 0.0 0.008075637078036156 9.445189564954568E-5 32-33 0.0 0.0 0.0 0.011806486956193211 9.445189564954568E-5 34-35 0.0 0.0 0.0 0.015348433043051175 9.445189564954568E-5 36-37 0.0 0.0 0.0 0.020165479721178006 9.445189564954568E-5 38-39 0.0 0.0 0.0 0.027202145947069156 9.445189564954568E-5 40-41 0.0 0.0 0.0 0.035183331129455764 9.445189564954568E-5 42-43 0.0 0.0 0.0 0.0527041577724465 9.445189564954568E-5 44-45 0.0 0.0 0.0 0.07423918998054291 9.445189564954568E-5 46-47 0.0 0.0 0.0 0.09761603415380546 9.445189564954568E-5 48-49 0.0 0.0 0.0 0.125573795266071 9.445189564954568E-5 50-51 0.0 0.0 0.0 0.16250448646504334 9.445189564954568E-5 52-53 0.0 0.0 0.0 0.22847913557625102 9.445189564954568E-5 54-55 0.0 0.0 0.0 0.27764134726183953 9.445189564954568E-5 56-57 0.0 0.0 0.0 0.3734155694504789 9.445189564954568E-5 58-59 0.0 0.0 0.0 0.5039008632903262 9.445189564954568E-5 60-61 0.0 0.0 0.0 0.6332527343823791 9.445189564954568E-5 62-63 0.0 0.0 0.0 0.8205036175076034 9.445189564954568E-5 64-65 0.0 0.0 0.0 0.9488165177475112 9.445189564954568E-5 66-67 0.0 0.0 0.0 1.18400173791488 9.445189564954568E-5 68-69 0.0 0.0 0.0 1.4514895063943933 9.445189564954568E-5 70-71 0.0 0.0 0.0 1.6799214160228195 9.445189564954568E-5 72-73 0.0 0.0 0.0 2.0576345467253527 1.889037912990914E-4 74-75 0.0 0.0 0.0 2.4227383493586716 2.833556869486371E-4 76-77 0.0 0.0 0.0 2.685456297107883 2.833556869486371E-4 78-79 0.0 0.0 0.0 2.990016434629843 2.833556869486371E-4 80-81 0.0 0.0 0.0 3.504590362128568 2.833556869486371E-4 82-83 0.0 0.0 0.0 3.9279709843776565 2.833556869486371E-4 84-85 0.0 0.0 0.0 4.5242458016132385 2.833556869486371E-4 86-87 0.0 0.0 0.0 5.231501596237036 2.833556869486371E-4 88-89 0.0 0.0 0.0 6.181120955097569 2.833556869486371E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2810 0.0 51.602036 1 GTATCAA 5450 0.0 44.575653 1 TCAACGC 6955 0.0 34.706726 4 ATCAACG 6965 0.0 34.653614 3 CAACGCA 7085 0.0 34.00284 5 TATCAAC 7175 0.0 33.640953 2 GGACCGA 85 1.6302485E-5 33.534904 6 AACGCAG 7260 0.0 33.3078 6 CGACCCG 80 4.4841092E-4 29.69799 5 ACGCAGA 8235 0.0 29.018112 7 CGCAGAG 8215 0.0 28.973099 8 GTACATG 4270 0.0 27.72329 1 GCAGAGT 8515 0.0 27.672047 9 TACATGG 4330 0.0 26.774935 2 ACATGGG 4370 0.0 25.44136 3 TATAACG 115 1.2789972E-4 24.790194 2 GAGTACT 5640 0.0 24.045298 12-13 ATGGGCG 140 1.7744456E-5 23.758392 5 AGAGTAC 7815 0.0 23.372293 10-11 GTGGTAT 1475 0.0 23.20672 1 >>END_MODULE