FastQCFastQC Report
Thu 26 May 2016
SRR937451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058740
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51910.49029979031679166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.40878780437123374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.26285962559268566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.1093752951621739No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32150.043.005921
GTATCAA54300.037.8007281
TAACGCA550.002580144234.543794
GGACCGC1001.2995552E-633.2483986
TGGACCG1456.6393113E-1032.7570425
GACGCAT600.003949918331.668138
TATCAAC69300.029.67752
TCAACGC69000.029.6688584
ATCAACG69200.029.5144733
CAACGCA69850.029.239825
AACGCAG71800.028.5118566
GTATACG700.00839325127.1415484
GGACCGT1257.3929477E-626.5987176
ACGCAGA82350.024.8038187
CGCAGAG82250.024.602968
GTACATG46700.023.197131
GCAGAGT86150.022.882669
CCTATCC2103.4473487E-822.6179583
GAGTACT47450.022.32453212-13
AGTACTT49750.021.57888812-13
Produced by FastQC (version 0.11.5)