##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937451_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1058740 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.254141715624236 33.0 31.0 34.0 31.0 34.0 2 32.46168558852976 34.0 31.0 34.0 31.0 34.0 3 32.52116855885298 34.0 31.0 34.0 31.0 34.0 4 36.14042824489488 37.0 35.0 37.0 35.0 37.0 5 36.0668237716531 37.0 35.0 37.0 35.0 37.0 6 36.10787634357821 37.0 35.0 37.0 35.0 37.0 7 36.05040425411338 37.0 35.0 37.0 35.0 37.0 8 36.0307403139581 37.0 35.0 37.0 35.0 37.0 9 37.72612539433666 39.0 37.0 39.0 35.0 39.0 10-11 37.648271530309614 39.0 37.0 39.0 35.0 39.0 12-13 37.54384409770104 39.0 37.0 39.0 35.0 39.0 14-15 38.778212308971035 40.0 38.0 41.0 35.0 41.0 16-17 38.73374813457506 40.0 38.0 41.0 35.0 41.0 18-19 38.723559608591344 40.0 38.0 41.0 35.0 41.0 20-21 38.62365594952491 40.0 38.0 41.0 34.0 41.0 22-23 38.566776073445794 40.0 38.0 41.0 34.0 41.0 24-25 38.60029799573077 40.0 38.0 41.0 34.0 41.0 26-27 38.45153153748796 40.0 38.0 41.0 34.0 41.0 28-29 38.3035258892646 40.0 38.0 41.0 34.0 41.0 30-31 38.342194967603 40.0 38.0 41.0 34.0 41.0 32-33 38.24930719534541 40.0 38.0 41.0 34.0 41.0 34-35 38.128652927064245 40.0 38.0 41.0 34.0 41.0 36-37 37.9700195515424 40.0 37.0 41.0 33.0 41.0 38-39 37.8584303039462 40.0 37.0 41.0 33.0 41.0 40-41 37.66819049058314 40.0 37.0 41.0 33.0 41.0 42-43 37.46594914709938 40.0 37.0 41.0 33.0 41.0 44-45 37.148276725163875 40.0 36.5 41.0 32.0 41.0 46-47 36.77887205546215 40.0 36.0 41.0 31.0 41.0 48-49 36.44012410979089 39.0 36.0 41.0 30.0 41.0 50-51 36.1919626159397 39.0 36.0 41.0 30.0 41.0 52-53 35.95912546989818 39.0 35.0 40.5 29.0 41.0 54-55 35.742855186353594 39.0 35.0 40.0 28.5 41.0 56-57 35.45368126263294 38.5 35.0 40.0 28.0 41.0 58-59 35.203858359937286 38.0 35.0 40.0 28.0 41.0 60-61 34.85528741711846 38.0 34.0 40.0 26.5 41.0 62-63 34.486675198821246 37.5 34.0 40.0 26.0 41.0 64-65 34.06583060997034 37.0 33.5 40.0 26.0 41.0 66-67 33.63684615675237 36.5 33.0 39.0 25.0 40.5 68-69 33.18450516651869 36.0 32.5 39.0 23.0 40.0 70-71 32.74515131193683 35.5 32.0 38.5 22.5 40.0 72-73 32.26758977652681 35.0 31.5 38.0 21.5 40.0 74-75 31.780518824262803 35.0 31.0 37.0 20.0 39.0 76-77 30.463186901411113 33.5 29.5 35.5 19.5 38.5 78-79 31.09756597464911 34.0 31.0 36.0 19.5 38.5 80-81 30.905917411262443 34.0 31.0 36.0 18.0 37.5 82-83 30.50955144794756 34.0 30.5 35.0 16.5 37.0 84-85 30.084116024708614 34.0 30.0 35.0 10.0 36.5 86-87 29.71152690934507 34.0 30.0 35.0 7.0 36.0 88-89 29.280296390048548 34.0 29.0 35.0 2.0 36.0 90-91 28.746610593724615 34.0 29.0 35.0 2.0 35.0 92-93 28.248702230953775 33.5 29.0 35.0 2.0 35.0 94-95 27.772722764795887 33.0 27.5 35.0 2.0 35.0 96-97 27.112512042616693 33.0 26.5 35.0 2.0 35.0 98-99 26.171568090371572 32.5 25.0 34.0 2.0 35.0 100-101 23.24012505430984 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3360593979341715 1101 2 0.3763367820703323 1101 3 0.25323785491583095 1101 4 0.19440319354844604 1101 5 0.1303504004402143 1101 6 0.22074200265667088 1101 7 0.24314045297365539 1101 8 0.2294649995221576 1101 9 0.31763258132063754 1101 10-11 0.28389151191568374 1101 12-13 0.3533038276769531 1101 14-15 0.4385662896973983 1101 16-17 0.3344740219196538 1101 18-19 0.3327871497872721 1101 20-21 0.3182973351045604 1101 22-23 0.36805287825288957 1101 24-25 0.29675972064830347 1101 26-27 0.35722839287066677 1101 28-29 0.2220761529552746 1101 30-31 0.5333053889637185 1101 32-33 0.5438742581774676 1101 34-35 0.43498713685182366 1101 36-37 0.5276629451254777 1101 38-39 0.4320838918487908 1101 40-41 0.45224768741860544 1101 42-43 0.4186484394695995 1101 44-45 0.38036026227945996 1101 46-47 0.41549169020421317 1101 48-49 0.5197754881149592 1101 50-51 0.506044623261495 1101 52-53 0.4283585923925841 1101 54-55 0.6072473957644036 1101 56-57 0.4095831510016126 1101 58-59 0.6750409998085161 1101 60-61 0.6576915153519991 1101 62-63 0.8645542874030525 1101 64-65 0.8827643023602647 1101 66-67 0.8021092046592457 1101 68-69 0.8776595902321915 1101 70-71 0.934274118028469 1101 72-73 1.099355221008672 1101 74-75 0.983287041928687 1101 76-77 0.9336245976145179 1101 78-79 0.9990519139016065 1101 80-81 0.8743468406122723 1101 82-83 0.9292765804074001 1101 84-85 1.0250962059753874 1101 86-87 1.0908046863005048 1101 88-89 1.462593219760965 1101 90-91 1.4724525980867043 1101 92-93 1.5442204391055725 1101 94-95 1.6418066820922341 1101 96-97 1.9657365670542113 1101 98-99 2.2898676514700007 1101 100-101 2.9462999008794775 1102 1 0.22774931250767594 1102 2 0.2609472627537386 1102 3 0.19275487646370948 1102 4 0.18979475290073822 1102 5 0.08480048331666268 1102 6 0.12470528201406239 1102 7 0.11951734956934246 1102 8 0.04251612791690462 1102 9 0.1867234904115449 1102 10-11 0.22211872089184226 1102 12-13 0.19364680867911943 1102 14-15 0.3252369514339861 1102 16-17 0.3020203689807275 1102 18-19 0.3131252857046576 1102 20-21 0.28344664021740584 1102 22-23 0.18393022110638668 1102 24-25 0.07408956892065532 1102 26-27 0.13064468765582404 1102 28-29 0.07308775565832093 1102 30-31 0.20904961948775735 1102 32-33 0.2584834638385587 1102 34-35 0.40149357571450395 1102 36-37 0.33994498006108387 1102 38-39 0.2971860211360564 1102 40-41 0.15723761474233555 1102 42-43 0.2516771274716376 1102 44-45 0.11130530881777645 1102 46-47 0.1922885791497677 1102 48-49 0.4128067261616195 1102 50-51 0.39343898766287566 1102 52-53 0.44567237538378635 1102 54-55 0.447480724847658 1102 56-57 0.5537264635095767 1102 58-59 0.4929119675504552 1102 60-61 0.5249081416429533 1102 62-63 0.4108258671556939 1102 64-65 0.5737802401391718 1102 66-67 0.5901500053732534 1102 68-69 0.5165625610341849 1102 70-71 0.42731402622685977 1102 72-73 0.3290295803099639 1102 74-75 0.35921615068809487 1102 76-77 0.3524163864708214 1102 78-79 0.5176733597769108 1102 80-81 0.4367443925694303 1102 82-83 0.5953576718265019 1102 84-85 0.7457184168640758 1102 86-87 0.8592560441992667 1102 88-89 1.0433911287243731 1102 90-91 1.2057551608815515 1102 92-93 1.0527592635350018 1102 94-95 1.2061040172956012 1102 96-97 1.0587833603730914 1102 98-99 1.4133913031339063 1102 100-101 2.3694836315947647 1103 1 0.19866023380114228 1103 2 0.1279393310880934 1103 3 0.13362674955705955 1103 4 0.1929594874950027 1103 5 0.12648992810006376 1103 6 0.022413030641274645 1103 7 0.006337361771905137 1103 8 0.05312930668444693 1103 9 0.024008420246815376 1103 10-11 0.15634355310660908 1103 12-13 0.15444302588473136 1103 14-15 0.33453591421616835 1103 16-17 0.2589877271259411 1103 18-19 0.27333516978031014 1103 20-21 0.2424121679782303 1103 22-23 0.30135059938583453 1103 24-25 0.17956302834713256 1103 26-27 0.19800008728974916 1103 28-29 0.25795230721414697 1103 30-31 0.2504919623797619 1103 32-33 0.28604447665125576 1103 34-35 0.4192605067700228 1103 36-37 0.4911078842709671 1103 38-39 0.42540719258205684 1103 40-41 0.425928584846055 1103 42-43 0.6201795679841453 1103 44-45 0.6349425876463286 1103 46-47 0.6546580117306107 1103 48-49 0.7775034924825519 1103 50-51 0.6292949974249282 1103 52-53 0.5965091661098469 1103 54-55 0.6982967712174002 1103 56-57 0.741421399446125 1103 58-59 0.9160428400336755 1103 60-61 0.9389776962494238 1103 62-63 0.9847984113899884 1103 64-65 0.8599385683881096 1103 66-67 0.8933733122676983 1103 68-69 1.14060588013119 1103 70-71 1.2256050573433939 1103 72-73 0.8838782685344881 1103 74-75 0.686030671737516 1103 76-77 0.6384173016630115 1103 78-79 0.8201953243894771 1103 80-81 0.9911864303973772 1103 82-83 0.8267399170980063 1103 84-85 0.7329392832460186 1103 86-87 1.100990638464065 1103 88-89 1.1632483587426776 1103 90-91 1.3603671193928406 1103 92-93 1.2536454746393346 1103 94-95 1.5246476414566743 1103 96-97 1.5632873262059164 1103 98-99 2.0086503025237796 1103 100-101 2.583560812802819 1104 1 0.07899480960028171 1104 2 0.12536365759135037 1104 3 0.11531564798548288 1104 4 0.05821171503376377 1104 5 -0.07820674969993036 1104 6 -0.02246363019697384 1104 7 0.026882545995384532 1104 8 -0.031277660198256285 1104 9 -0.04352186485721177 1104 10-11 0.08523572557203352 1104 12-13 0.016910175577436348 1104 14-15 0.007267018091191346 1104 16-17 0.05637265943598635 1104 18-19 0.05651005382264884 1104 20-21 0.09198982558118018 1104 22-23 0.011964258831532959 1104 24-25 -0.026688476746627998 1104 26-27 0.028811188861332937 1104 28-29 -0.02818695714837105 1104 30-31 -0.07940364955209844 1104 32-33 0.10293673287841898 1104 34-35 0.026522649604729054 1104 36-37 -0.012273995970680573 1104 38-39 -0.03156479435111237 1104 40-41 0.061123304715387405 1104 42-43 0.06804246572153261 1104 44-45 -0.08284644270683117 1104 46-47 0.13973206234364 1104 48-49 0.2487863035265363 1104 50-51 0.033484654944111014 1104 52-53 0.07407345324508441 1104 54-55 0.10903227781887637 1104 56-57 0.13716682657864965 1104 58-59 -0.06066681560087517 1104 60-61 -0.061116535707768094 1104 62-63 0.26885933758542535 1104 64-65 0.41722570886701504 1104 66-67 0.30218687957549406 1104 68-69 0.45334996707587294 1104 70-71 0.45270276541279486 1104 72-73 0.3909495916558683 1104 74-75 0.34989350141848874 1104 76-77 0.4842008142115688 1104 78-79 0.45158542898694876 1104 80-81 0.45353094766799984 1104 82-83 0.1993900076653894 1104 84-85 0.19886265172437945 1104 86-87 0.46807777161381736 1104 88-89 0.7463714152085608 1104 90-91 0.6028769875728024 1104 92-93 0.533394634404381 1104 94-95 0.6423629876544155 1104 96-97 0.8839979398653632 1104 98-99 0.9151113428445861 1104 100-101 1.764007812562003 1105 1 0.04049797560927715 1105 2 0.01781489149888671 1105 3 0.01954969003048035 1105 4 0.04064875627044984 1105 5 0.005739315636638764 1105 6 0.09522114165804396 1105 7 0.018501453298007675 1105 8 0.05648433537423614 1105 9 0.08556167076463339 1105 10-11 0.003994627418833829 1105 12-13 -0.02929803052678892 1105 14-15 -0.027876813125395472 1105 16-17 0.04855820503669861 1105 18-19 0.053324245487822 1105 20-21 0.17664772438706677 1105 22-23 0.21248256159426404 1105 24-25 0.1280829759970601 1105 26-27 0.00572138533365063 1105 28-29 0.07104482840024673 1105 30-31 0.07511921145904665 1105 32-33 0.11038002819282156 1105 34-35 0.03918165717373512 1105 36-37 0.057797445081966714 1105 38-39 0.13390333122323028 1105 40-41 -0.16670583262416017 1105 42-43 -0.05160526942236032 1105 44-45 0.035716414231295346 1105 46-47 0.13279286455010464 1105 48-49 0.1704575942299016 1105 50-51 0.008405803280773227 1105 52-53 0.04657751053871806 1105 54-55 0.051399851651559914 1105 56-57 0.16411310185108618 1105 58-59 0.1478321931749491 1105 60-61 0.07244605095362999 1105 62-63 0.21561262270473947 1105 64-65 0.16016834357481713 1105 66-67 0.25883889263425885 1105 68-69 0.12520573735825735 1105 70-71 0.39992526349885793 1105 72-73 0.4271651015171969 1105 74-75 0.5574518843339824 1105 76-77 0.4922531749844836 1105 78-79 0.3416176861998821 1105 80-81 0.33911872412023314 1105 82-83 0.2903054412206849 1105 84-85 0.5628468323647731 1105 86-87 0.6668710174613004 1105 88-89 0.7158593728090565 1105 90-91 0.8340575563104622 1105 92-93 0.761495619845892 1105 94-95 0.622374766691248 1105 96-97 1.1012262583292873 1105 98-99 1.3165433066501322 1105 100-101 1.5206842762361816 1106 1 0.008466091337375303 1106 2 0.053692785737659676 1106 3 0.014813961669595699 1106 4 0.0900726130581404 1106 5 0.04906556829441655 1106 6 -0.02219742130520075 1106 7 -0.056033694117203936 1106 8 -0.05243539483888071 1106 9 -0.03744897451374385 1106 10-11 -0.31133673707553555 1106 12-13 -0.41689727709707824 1106 14-15 -0.17148393370346326 1106 16-17 -0.18584366660726914 1106 18-19 -0.2290268758101206 1106 20-21 0.012555343308541467 1106 22-23 0.023744981001449617 1106 24-25 0.05006629984342936 1106 26-27 0.01137059161385423 1106 28-29 0.11572537399982963 1106 30-31 -0.04093646469154777 1106 32-33 -0.05033597268476342 1106 34-35 -0.4704854364903568 1106 36-37 -0.04203654155406156 1106 38-39 0.08309636675028997 1106 40-41 0.02475398294125597 1106 42-43 0.11640440019890974 1106 44-45 -0.021635035655812374 1106 46-47 0.013585259884344225 1106 48-49 -0.09755440079172217 1106 50-51 0.03121747517285911 1106 52-53 -0.05769799076198723 1106 54-55 -0.020159288840027045 1106 56-57 -0.07628425851620335 1106 58-59 -0.19711061714214395 1106 60-61 -0.16637964209149914 1106 62-63 -0.07791053069989573 1106 64-65 -0.12918223277061003 1106 66-67 -0.13951533054553522 1106 68-69 -0.055971258972668636 1106 70-71 0.03210152480105677 1106 72-73 0.16969525842105782 1106 74-75 0.021720085899683994 1106 76-77 -0.10442130030914853 1106 78-79 0.04876586576527586 1106 80-81 -0.047662588153734475 1106 82-83 -0.30896033889552754 1106 84-85 -0.09424508262263132 1106 86-87 -0.17562658383768692 1106 88-89 -0.06936260687503548 1106 90-91 -0.08431533073473574 1106 92-93 0.07492420022942525 1106 94-95 -0.326563485841163 1106 96-97 -0.14896204854672135 1106 98-99 0.43887048643491866 1106 100-101 1.0851423517955148 1107 1 0.07830873854373266 1107 2 0.047221217251994574 1107 3 0.10197492744077152 1107 4 0.08445575544958928 1107 5 0.09086562067152215 1107 6 0.033533376038420215 1107 7 0.04603259548645866 1107 8 0.038857107809285196 1107 9 0.03206664154909333 1107 10-11 0.025383518399635818 1107 12-13 0.012861384342301108 1107 14-15 0.10491145346362174 1107 16-17 0.2612183298983197 1107 18-19 0.1445430143934061 1107 20-21 0.04074712166742245 1107 22-23 0.10017906468233662 1107 24-25 0.06452211871769009 1107 26-27 0.016191369426806546 1107 28-29 0.05038559385335617 1107 30-31 0.06252719902141024 1107 32-33 0.23618760808476225 1107 34-35 0.19460600845309983 1107 36-37 0.28488711165741876 1107 38-39 0.2752851431977916 1107 40-41 0.32657245850707994 1107 42-43 0.346933995214485 1107 44-45 0.3774667361754638 1107 46-47 0.5860233096321608 1107 48-49 0.6274254366308014 1107 50-51 0.6767313505440242 1107 52-53 0.5637888674068208 1107 54-55 0.5567321172766242 1107 56-57 0.7188339873460734 1107 58-59 0.6768247401361265 1107 60-61 0.7885180727295236 1107 62-63 0.8682120655886223 1107 64-65 0.7976650697436227 1107 66-67 0.870797225235421 1107 68-69 0.8407468336670476 1107 70-71 0.7613164806779125 1107 72-73 0.8129023264630817 1107 74-75 0.9175176705597075 1107 76-77 0.948698459537944 1107 78-79 0.7999779005113545 1107 80-81 0.805583814829415 1107 82-83 0.8809874273254117 1107 84-85 0.7297795893364665 1107 86-87 0.9203375130938234 1107 88-89 1.1628145215309438 1107 90-91 1.2444324005681366 1107 92-93 1.2915282619301678 1107 94-95 1.3620292509404628 1107 96-97 1.3375508483368428 1107 98-99 1.7404661638910106 1107 100-101 2.1555895505978775 1108 1 -0.012286636334359002 1108 2 0.10937450091481793 1108 3 -0.07300108620479762 1108 4 0.08179060495742618 1108 5 0.18678734816284503 1108 6 0.16525695847447963 1108 7 0.07153117604705272 1108 8 0.15113693676585171 1108 9 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0.8540381466698754 2316 86-87 0.9362208626625375 2316 88-89 1.0006784915163784 2316 90-91 1.094713224489773 2316 92-93 0.8545127113255973 2316 94-95 1.3190328098652593 2316 96-97 1.5572252404744127 2316 98-99 1.8736598086222251 2316 100-101 2.168771557291361 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 8.0 5 24.0 6 38.0 7 105.0 8 270.0 9 433.0 10 774.0 11 1275.0 12 2028.0 13 2940.0 14 3882.0 15 5642.0 16 7817.0 17 11370.0 18 10116.0 19 5998.0 20 5362.0 21 5524.0 22 5663.0 23 6157.0 24 7318.0 25 8271.0 26 10108.0 27 12476.0 28 15099.0 29 19017.0 30 23893.0 31 29884.0 32 38500.0 33 51256.0 34 71063.0 35 103309.0 36 159276.0 37 238796.0 38 180844.0 39 14203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57154035006152 20.755348162091977 15.214502926629208 21.458608561217297 2 15.689498838241683 21.73838713942989 39.670929595556984 22.901184426771444 3 19.12943687779814 26.28700153012071 30.215917033454858 24.367644558626292 4 12.868787426563651 17.93518710920528 37.75733418969719 31.43869127453388 5 13.232470254427714 38.59637357624094 34.25928637073321 13.911869798598131 6 30.745131004779264 38.04144549181102 17.365736630334172 13.847686873075544 7 27.72597616034154 34.022611783818505 21.077601677465665 17.173810378374295 8 26.454937000585605 35.64652322572114 19.768403951867313 18.130135821825945 9 26.67132629351871 17.124128681262633 20.765627066135217 35.43891795908344 10-11 24.547197612256078 27.944773976613714 27.421793830402173 20.086234580728036 12-13 25.521993877286924 25.389328796870824 28.12551656259062 20.963160763251633 14-15 22.516340177947374 26.72700568600412 26.60313202485974 24.15352211118877 16-17 21.4612180516463 30.25232823922776 27.135699038479704 21.15075467064624 18-19 21.273683812834125 28.189073804711263 30.217050456202653 20.320191926251958 20-21 22.009720998281917 27.415349769867735 30.530895716508034 20.044033515342306 22-23 21.830573182832048 27.283859837531534 30.328408085736807 20.557158893899615 24-25 21.651208039745356 27.763756918601356 30.25766477133196 20.327370270321328 26-27 21.491629290611364 28.00094264639001 30.15448349731256 20.352944565686066 28-29 21.07750557402939 27.95770680109511 30.524192539089047 20.440595085786452 30-31 21.244359437537632 27.71314891557161 30.451315836078347 20.591175810812405 32-33 21.130174136319653 28.048873211965734 30.127004801464384 20.693947850250225 34-35 21.69564765664847 27.802671099608965 30.0310746736687 20.470606570073862 36-37 21.66636756899711 27.7276290685154 29.824036118404894 20.781967244082587 38-39 21.470616015263428 28.18765702627652 29.91551277934148 20.426214179118574 40-41 21.512363753140527 27.974620775638968 30.159954285282502 20.353061185938 42-43 21.331226228356083 28.2580347152787 29.681086261105634 20.729652795259582 44-45 21.489034134915087 28.386572718514458 29.43064397302454 20.693749173545914 46-47 21.636473544024028 28.606267827795307 28.76343578215615 20.99382284602452 48-49 21.727147363847592 28.848159132553793 28.643859682263823 20.78083382133479 50-51 21.766061544855205 28.898501992935 28.336513213820204 20.998923248389595 52-53 22.070810586168466 28.99696809414965 28.17282807865954 20.759393241022348 54-55 21.604076543816234 29.051844645522035 28.461661975555852 20.88241683510588 56-57 21.539282543400645 29.48042012203185 28.203949978276064 20.776347356291442 58-59 21.745234901864478 28.803341708068082 28.371649319001836 21.079774071065607 60-61 21.767053289759524 28.982280824376144 28.600175680525908 20.65049020533842 62-63 21.653380433345298 28.876305797457352 28.82893817178911 20.641375597408242 64-65 21.49380395564539 28.713659633148836 28.711817821183672 21.0807185900221 66-67 21.377486446152975 29.23125602130835 28.342841490828718 21.04841604170996 68-69 21.435007650603545 28.910639061525966 28.51984434327597 21.13450894459452 70-71 21.391239299185493 29.14149325683986 28.389041874795453 21.0782255691792 72-73 21.52924736230403 29.030074999775675 28.201209649426463 21.239467988493832 74-75 21.44889207926403 29.31758505393203 27.956958271152505 21.276564595651433 76-77 21.5599675085479 28.867427319266298 28.351011579802403 21.2215935923834 78-79 21.558503504165326 29.012741560723125 27.912849235884163 21.515905699227382 80-81 21.3304494021195 29.457137729754233 27.901042748927974 21.31137011919829 82-83 21.47396239674027 29.18507695010475 28.02506380237604 21.315896850778945 84-85 21.18213270692777 29.19713923828252 27.757502085027564 21.863225969762144 86-87 21.039915371101497 29.045421916617865 28.166121994068423 21.748540718212215 88-89 21.142490129776906 28.734769631826513 28.21046715907588 21.912273079320702 90-91 21.016492858762184 28.83775409959949 28.32152573112673 21.8242273105116 92-93 21.116757837607576 28.885816011799093 28.074274209887268 21.923151940706063 94-95 20.913916542305003 28.776753499442737 28.151009690764496 22.15832026748777 96-97 20.862156903489055 29.061337061034813 27.951621739048306 22.12488429642783 98-99 20.66521808327009 29.567252632975848 27.59848895215094 22.169040331603124 100-101 20.53006626182513 29.665261828437185 27.798557250188573 22.006114659549112 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 360.0 1 253.5 2 168.0 3 205.0 4 214.5 5 234.5 6 383.0 7 685.5 8 1057.5 9 1328.5 10 1446.5 11 1537.5 12 1669.5 13 1791.0 14 1878.0 15 1984.5 16 2120.0 17 2255.5 18 2368.5 19 2481.5 20 2680.5 21 3025.0 22 3469.5 23 4016.0 24 4747.0 25 5785.0 26 7360.0 27 9211.0 28 10601.5 29 12559.5 30 14919.5 31 16931.0 32 19715.5 33 22948.0 34 26627.0 35 29182.5 36 32109.0 37 35534.0 38 37037.5 39 37046.5 40 36738.0 41 37269.5 42 37824.5 43 38876.5 44 40155.0 45 40122.5 46 38606.5 47 37718.5 48 39451.5 49 39351.5 50 37388.0 51 35222.5 52 32116.5 53 30202.0 54 28291.0 55 25291.5 56 21934.5 57 18965.5 58 16600.0 59 14505.5 60 12491.0 61 10204.0 62 7876.5 63 5974.0 64 4257.0 65 3112.0 66 2286.0 67 1803.0 68 1280.0 69 756.5 70 587.5 71 445.5 72 337.5 73 286.5 74 221.5 75 166.5 76 116.5 77 59.5 78 32.0 79 26.0 80 13.5 81 7.0 82 6.0 83 3.5 84 2.5 85 2.0 86 2.5 87 1.5 88 0.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049681697111661025 2 0.0 3 0.0 4 0.0 5 0.0010389708521450025 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005572661843323196 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.445189564954568E-5 22-23 0.0010861967999697755 24-25 0.0 26-27 0.0017473600695165953 28-29 0.0033530422955588717 30-31 2.3612973912386423E-4 32-33 4.722594782477284E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009397963617129796 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 4.722594782477284E-5 72-73 3.305816347734099E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8890379129909136E-4 84-85 9.445189564954568E-5 86-87 0.0 88-89 0.0 90-91 0.009445189564954567 92-93 0.0013695524869184125 94-95 0.0 96-97 0.0 98-99 4.250335304229556E-4 100-101 0.012892683756162985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1058740.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.909631399754936 #Duplication Level Percentage of deduplicated Percentage of total 1 79.42469857191371 44.40605621192338 2 11.671012116588454 13.05043971001068 3 3.652939049060735 6.127034272762709 4 1.5949659297565695 3.5669582891142895 5 0.8508779680436143 2.3786136779745464 6 0.5466225874701848 1.8336880428142996 7 0.38361297174230524 1.5013361895193824 8 0.2677161401096443 1.1974328570636288 9 0.19354517298820925 0.9738935352875325 >10 1.2392080570757529 13.120489172471139 >50 0.11291887234301087 4.35228828845617 >100 0.05950246472835128 5.600554048500203 >500 0.0017000701281811128 0.6118387438963295 >1k 5.100210384543339E-4 0.7859708829079927 >5k 1.7000701281811128E-4 0.4934060772977857 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5191 0.49029979031679166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4328 0.40878780437123374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2783 0.26285962559268566 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1158 0.1093752951621739 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 9.445189564954568E-5 0.0 8 0.0 0.0 0.0 9.445189564954568E-5 0.0 9 0.0 0.0 0.0 1.8890379129909136E-4 0.0 10-11 0.0 0.0 0.0 2.833556869486371E-4 0.0 12-13 9.445189564954568E-5 0.0 0.0 2.833556869486371E-4 0.0 14-15 9.445189564954568E-5 0.0 0.0 2.833556869486371E-4 0.0 16-17 9.445189564954568E-5 0.0 0.0 3.778075825981828E-4 0.0 18-19 9.445189564954568E-5 0.0 0.0 6.611632695468199E-4 0.0 20-21 9.445189564954568E-5 0.0 0.0 8.028411130211383E-4 0.0 22-23 9.445189564954568E-5 0.0 0.0 0.0010389708521450025 0.0 24-25 9.445189564954568E-5 0.0 0.0 0.0012751005912688667 0.0 26-27 9.445189564954568E-5 0.0 0.0 0.002077941704290005 0.0 28-29 9.445189564954568E-5 0.0 0.0 0.003966979617280919 0.0 30-31 9.445189564954568E-5 0.0 0.0 0.007886733286737065 0.0 32-33 9.445189564954568E-5 0.0 0.0 0.01180648695619321 0.0 34-35 9.445189564954568E-5 0.0 0.0 0.015537336834350265 0.0 36-37 9.445189564954568E-5 0.0 0.0 0.020448835408126642 0.0 38-39 9.445189564954568E-5 0.0 0.0 0.027485501634017792 0.0 40-41 9.445189564954568E-5 0.0 0.0 0.03556113871205395 0.0 42-43 9.445189564954568E-5 0.0 0.0 0.05341254698981808 0.0 44-45 9.445189564954568E-5 0.0 0.0 0.07518370893703837 0.0 46-47 9.445189564954568E-5 0.0 0.0 0.09870223095377526 0.0 48-49 9.445189564954568E-5 0.0 0.0 0.12661276611821598 0.0 50-51 9.445189564954568E-5 0.0 0.0 0.16387403895196176 0.0 52-53 9.445189564954568E-5 0.0 0.0 0.23050985133271626 0.0 54-55 9.445189564954568E-5 0.0 0.0 0.2800970965487277 0.0 56-57 9.445189564954568E-5 0.0 0.0 0.3765324820069139 0.0 58-59 9.445189564954568E-5 0.0 0.0 0.5078678429076071 0.0 60-61 9.445189564954568E-5 0.0 0.0 0.636983584260536 0.0 62-63 9.445189564954568E-5 0.0 0.0 0.8260290534031018 0.0 64-65 9.445189564954568E-5 0.0 0.0 0.9545780833821336 0.0 66-67 9.445189564954568E-5 0.0 0.0 1.19122730793207 0.0 68-69 9.445189564954568E-5 0.0 0.0 1.460698566220224 0.0 70-71 9.445189564954568E-5 0.0 0.0 1.6890832499008255 0.0 72-73 9.445189564954568E-5 0.0 0.0 2.0726996240814555 0.0 74-75 9.445189564954568E-5 0.0 0.0 2.4426204733929007 4.722594782477284E-5 76-77 9.445189564954568E-5 0.0 0.0 2.709730434289816 9.445189564954568E-5 78-79 9.445189564954568E-5 0.0 0.0 3.017265806524737 9.445189564954568E-5 80-81 9.445189564954568E-5 0.0 0.0 3.537979107240682 9.445189564954568E-5 82-83 9.445189564954568E-5 0.0 0.0 3.967262972967867 9.445189564954568E-5 84-85 9.445189564954568E-5 0.0 0.0 4.571566201333661 9.445189564954568E-5 86-87 9.445189564954568E-5 0.0 0.0 5.2897784158528065 9.445189564954568E-5 88-89 9.445189564954568E-5 0.0 0.0 6.259468802538867 9.445189564954568E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3215 0.0 43.00592 1 GTATCAA 5430 0.0 37.800728 1 TAACGCA 55 0.0025801442 34.54379 4 GGACCGC 100 1.2995552E-6 33.248398 6 TGGACCG 145 6.6393113E-10 32.757042 5 GACGCAT 60 0.0039499183 31.66813 8 TATCAAC 6930 0.0 29.6775 2 TCAACGC 6900 0.0 29.668858 4 ATCAACG 6920 0.0 29.514473 3 CAACGCA 6985 0.0 29.23982 5 AACGCAG 7180 0.0 28.511856 6 GTATACG 70 0.008393251 27.141548 4 GGACCGT 125 7.3929477E-6 26.598717 6 ACGCAGA 8235 0.0 24.803818 7 CGCAGAG 8225 0.0 24.60296 8 GTACATG 4670 0.0 23.19713 1 GCAGAGT 8615 0.0 22.88266 9 CCTATCC 210 3.4473487E-8 22.617958 3 GAGTACT 4745 0.0 22.324532 12-13 AGTACTT 4975 0.0 21.578888 12-13 >>END_MODULE