FastQCFastQC Report
Thu 26 May 2016
SRR937450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017986
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77770.7639594257681344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57870.5684754014298821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38810.38124296404862146No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14690.1443045385692927No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.1349723866536475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.1062882986602959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.050.663271
GTATCAA50500.043.6074871
ATCAACG65100.033.228393
TCAACGC65800.033.0177764
TATCAAC66100.032.872782
CAACGCA66450.032.551725
AACGCAG67950.031.7350436
ACGCAGA74700.028.485917
CGCAGAG75850.028.0540228
GCAGAGT79150.026.524319
GTACATG41650.026.151221
TACATGG42950.024.6867182
ACATGGG42050.024.421183
GTGGTAT15600.022.8669431
GAGTACT53850.022.80066712-13
AGAGTAC74100.022.59506410-11
TGGTATC14050.021.996762
AGTACTT56300.021.85063612-13
CAGAGTA78550.021.46618710-11
GTACTTT57400.021.26639714-15
Produced by FastQC (version 0.11.5)