##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937450_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1017986 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.753052596008196 33.0 31.0 34.0 30.0 34.0 2 31.854977376899093 33.0 31.0 34.0 30.0 34.0 3 31.88105828567387 33.0 31.0 34.0 30.0 34.0 4 35.50138803480598 37.0 35.0 37.0 33.0 37.0 5 35.50203932077651 37.0 35.0 37.0 33.0 37.0 6 35.5535616403369 37.0 35.0 37.0 33.0 37.0 7 35.499102148752534 37.0 35.0 37.0 33.0 37.0 8 35.49411092097534 37.0 35.0 37.0 33.0 37.0 9 37.126286609049636 39.0 37.0 39.0 34.0 39.0 10-11 37.00816808875564 39.0 37.0 39.0 33.0 39.0 12-13 36.88033627181513 39.0 37.0 39.0 33.0 39.0 14-15 38.05180719577676 40.0 38.0 41.0 33.0 41.0 16-17 38.02672924774997 40.0 38.0 41.0 33.0 41.0 18-19 38.03867587569967 40.0 38.0 41.0 33.0 41.0 20-21 37.9998786820251 40.0 38.0 41.0 33.0 41.0 22-23 37.85711640435133 40.0 38.0 41.0 33.0 41.0 24-25 37.70600676237198 40.0 37.5 41.0 32.5 41.0 26-27 37.54921629570544 40.0 37.0 41.0 32.0 41.0 28-29 37.581424498961674 40.0 37.0 41.0 32.0 41.0 30-31 37.384747432675894 40.0 37.0 41.0 32.0 41.0 32-33 37.16612114508451 39.5 36.0 41.0 31.5 41.0 34-35 37.02712414512577 39.5 36.0 41.0 31.0 41.0 36-37 37.01230616138139 40.0 36.0 41.0 31.0 41.0 38-39 36.91818404182376 40.0 36.0 41.0 31.0 41.0 40-41 36.72483609794241 40.0 36.0 41.0 30.5 41.0 42-43 36.462132092189876 39.0 36.0 41.0 30.0 41.0 44-45 36.185003526571094 39.0 35.0 41.0 30.0 41.0 46-47 35.75450055305279 39.0 35.0 41.0 27.5 41.0 48-49 35.37907790480419 39.0 35.0 41.0 26.0 41.0 50-51 34.49203623625472 38.0 34.0 40.0 24.5 40.5 52-53 34.572089400050686 38.5 34.5 40.0 24.0 40.5 54-55 34.89894310923726 39.0 35.0 40.0 24.0 41.0 56-57 34.71639934144477 39.0 35.0 40.0 23.5 41.0 58-59 34.50424760261929 38.5 34.0 40.0 23.0 41.0 60-61 34.23844139310364 38.0 34.0 40.0 22.0 41.0 62-63 33.92740420791642 38.0 34.0 40.0 21.5 41.0 64-65 33.570404209881076 37.5 34.0 40.0 19.5 41.0 66-67 33.20409318006338 37.0 33.0 40.0 18.0 41.0 68-69 32.74600731247777 36.0 32.5 39.0 14.0 41.0 70-71 32.321455304886314 36.0 32.0 39.0 10.0 40.5 72-73 31.930873803765472 35.5 32.0 38.5 7.5 40.0 74-75 31.476179436652373 35.0 31.0 37.5 6.5 40.0 76-77 31.04443332226573 35.0 31.0 37.0 2.0 39.0 78-79 30.573275074509866 35.0 31.0 36.5 2.0 39.0 80-81 30.09404746234231 34.5 30.0 36.0 2.0 38.5 82-83 29.62472568385027 34.0 30.0 36.0 2.0 37.0 84-85 29.185658250702858 34.0 29.0 35.0 2.0 37.0 86-87 28.771411885821614 34.0 29.0 35.0 2.0 36.5 88-89 28.388163000277018 34.0 29.0 35.0 2.0 36.0 90-91 27.988333336607774 34.0 28.0 35.0 2.0 36.0 92-93 27.55465743143815 34.0 27.0 35.0 2.0 35.0 94-95 27.0766041969143 33.5 26.0 35.0 2.0 35.0 96-97 26.510478533103598 33.0 25.0 35.0 2.0 35.0 98-99 25.79759397476979 33.0 23.5 35.0 2.0 35.0 100-101 23.933369417654074 30.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19771288568082568 1101 2 0.22888252283541277 1101 3 0.1625055578503236 1101 4 0.16816172288155684 1101 5 0.22633613994508295 1101 6 0.25518737516601675 1101 7 0.16238187955858052 1101 8 0.27292652522437777 1101 9 0.20432235661198916 1101 10-11 0.2147556132913948 1101 12-13 0.23174912386021873 1101 14-15 0.41536992961280106 1101 16-17 0.5371078871497517 1101 18-19 0.33186828745525077 1101 20-21 0.3588346273124827 1101 22-23 0.3128300329280691 1101 24-25 0.24636438649186942 1101 26-27 0.45133740970624814 1101 28-29 0.28821022376057925 1101 30-31 0.2860084427909797 1101 32-33 0.27132367544509606 1101 34-35 0.2741204672259627 1101 36-37 0.3924127190648008 1101 38-39 0.4523806715447307 1101 40-41 0.40978985365011056 1101 42-43 0.563182618583177 1101 44-45 0.7423060524968861 1101 46-47 0.9071556004013175 1101 48-49 1.055452706887884 1101 50-51 1.022431893536357 1101 52-53 0.9888665888872765 1101 54-55 1.0937807944815034 1101 56-57 1.04073396163907 1101 58-59 1.0854167370793917 1101 60-61 1.019183357216015 1101 62-63 1.066445399158475 1101 64-65 0.9191217685572823 1101 66-67 1.044169440810677 1101 68-69 1.150994843329748 1101 70-71 1.3650698457140962 1101 72-73 1.2807594772064554 1101 74-75 1.2784397220660644 1101 76-77 1.3250550978682476 1101 78-79 1.444392209306649 1101 80-81 1.1834479513899332 1101 82-83 1.1173699031007907 1101 84-85 1.2675808603201304 1101 86-87 1.1861785789502157 1101 88-89 1.2630697145060843 1101 90-91 1.295885324577288 1101 92-93 1.3909378366932152 1101 94-95 1.4087630166796643 1101 96-97 1.2793939295684638 1101 98-99 1.4335179666839046 1101 100-101 1.822039633001264 1102 1 0.09135291084381336 1102 2 -0.011574860407606025 1102 3 0.12719816026035602 1102 4 0.034798726547720094 1102 5 -0.07920919788657699 1102 6 -0.03196184615915598 1102 7 0.07482083129173844 1102 8 -0.023334377719045563 1102 9 -0.042099227939992545 1102 10-11 0.10729022820118672 1102 12-13 0.04698339204156099 1102 14-15 0.09262173688588149 1102 16-17 0.2434713591871187 1102 18-19 0.279757895587629 1102 20-21 0.0789302989160987 1102 22-23 0.05871629301780956 1102 24-25 0.14932571461900324 1102 26-27 0.17001397639933202 1102 28-29 0.15998052764201276 1102 30-31 0.31403176195432536 1102 32-33 0.16361453970441886 1102 34-35 0.2573065178628937 1102 36-37 0.40310277568152486 1102 38-39 0.440601005278765 1102 40-41 0.5027321662770632 1102 42-43 0.4696550515854554 1102 44-45 0.6362332273025544 1102 46-47 0.9356864349639338 1102 48-49 0.859119458245793 1102 50-51 0.7243962667558819 1102 52-53 0.8019987447174728 1102 54-55 0.6510969586678996 1102 56-57 0.6534150207598728 1102 58-59 0.6818176965763385 1102 60-61 0.6725039910772566 1102 62-63 0.4723433222006861 1102 64-65 0.49833693781124566 1102 66-67 0.6788818087228918 1102 68-69 0.5138156851269429 1102 70-71 0.6607724606508469 1102 72-73 0.8054296975342901 1102 74-75 0.824973554108599 1102 76-77 0.6413347340077244 1102 78-79 0.7057740163648418 1102 80-81 0.6599387594412498 1102 82-83 0.6319372172551354 1102 84-85 0.6954664824868182 1102 86-87 0.580883775494982 1102 88-89 0.7614070696198105 1102 90-91 0.6908780921141648 1102 92-93 0.8401131926386896 1102 94-95 0.7368435972317755 1102 96-97 1.0385824021219712 1102 98-99 1.003636201549984 1102 100-101 1.2583528759719975 1103 1 -0.019248966300210668 1103 2 -0.04081479918140829 1103 3 -0.06204197538224676 1103 4 -0.1019399448831777 1103 5 -0.0916901877429126 1103 6 -0.04461755707701087 1103 7 -0.03669057971617917 1103 8 -0.10585242812729945 1103 9 -0.015026814672999933 1103 10-11 0.08599862121037916 1103 12-13 0.09231120320500708 1103 14-15 0.10689029546173145 1103 16-17 -0.15433109281381263 1103 18-19 -0.1331512839124187 1103 20-21 -0.21967045607575386 1103 22-23 -0.33702592697323297 1103 24-25 -0.2907624333869592 1103 26-27 -0.09999576860635528 1103 28-29 -0.10290256674181109 1103 30-31 -0.1895099077884126 1103 32-33 0.012098649931267857 1103 34-35 0.021995391330392522 1103 36-37 -0.0152549939918174 1103 38-39 -0.07928618889785355 1103 40-41 0.050951734051608355 1103 42-43 0.2709607839127912 1103 44-45 0.327494467280701 1103 46-47 0.332772776698647 1103 48-49 0.40074588985111603 1103 50-51 0.2558608229889998 1103 52-53 0.32796992312490403 1103 54-55 0.23956487643580004 1103 56-57 0.26305888509758546 1103 58-59 0.2829570747662231 1103 60-61 0.48111478637800076 1103 62-63 0.2900821855786617 1103 64-65 0.4762959989439324 1103 66-67 0.27821909911914133 1103 68-69 0.2537490450375657 1103 70-71 0.44326811723291826 1103 72-73 0.34558010529879724 1103 74-75 0.41160124075252824 1103 76-77 0.450218192598836 1103 78-79 0.39272186089755223 1103 80-81 0.3818098989670915 1103 82-83 0.3839538920426442 1103 84-85 0.3283696574884196 1103 86-87 0.5551692647877822 1103 88-89 0.5818130130889365 1103 90-91 0.5572044218398808 1103 92-93 0.8616188944513574 1103 94-95 0.9277453055608049 1103 96-97 0.6078738220874236 1103 98-99 0.7697262887136453 1103 100-101 1.090917928806661 1104 1 0.21479858139732144 1104 2 0.2328641539701266 1104 3 0.18688920032447243 1104 4 0.2197412058510153 1104 5 0.30369488867302863 1104 6 0.17056574129478008 1104 7 0.27459700394179265 1104 8 0.1996015724720408 1104 9 0.34601104368280744 1104 10-11 0.22817972199950987 1104 12-13 0.3283590505728995 1104 14-15 0.529423009476325 1104 16-17 0.4177346348857185 1104 18-19 0.39497277961769583 1104 20-21 0.3910028033388926 1104 22-23 0.5110237184168369 1104 24-25 0.4451455678117213 1104 26-27 0.4242850225234065 1104 28-29 0.31099214907079187 1104 30-31 0.43001611358807423 1104 32-33 0.25808556432543384 1104 34-35 0.628216048607392 1104 36-37 0.623285111891704 1104 38-39 0.5103452165561464 1104 40-41 0.7377112985306837 1104 42-43 0.837355250000094 1104 44-45 0.6762433435168376 1104 46-47 0.7632018047677747 1104 48-49 0.874227560013928 1104 50-51 0.6190388271843901 1104 52-53 0.6802474178806719 1104 54-55 0.7361386452900618 1104 56-57 0.8584139944772673 1104 58-59 0.8321979098476433 1104 60-61 0.9818739512965919 1104 62-63 0.7269491319376655 1104 64-65 0.7108055384504723 1104 66-67 0.6923160924265019 1104 68-69 0.7209925771522379 1104 70-71 0.6743389393286066 1104 72-73 0.821191932289107 1104 74-75 0.8367760484640421 1104 76-77 0.7727758791436337 1104 78-79 0.8243570621761442 1104 80-81 0.8470606231704672 1104 82-83 0.9135413458434627 1104 84-85 1.1373397903422777 1104 86-87 1.0845319658166481 1104 88-89 1.0434202451336851 1104 90-91 0.974542849569378 1104 92-93 1.0583499902459828 1104 94-95 0.8891402710987109 1104 96-97 1.0279217693454363 1104 98-99 1.2298726277407148 1104 100-101 1.2994535396347509 1105 1 0.07599098371358437 1105 2 0.07361790607850338 1105 3 0.11143287733696994 1105 4 0.2289573724156213 1105 5 0.15329577392351013 1105 6 0.15671973620244728 1105 7 0.2633240266424508 1105 8 0.20248293386695337 1105 9 0.204065318991411 1105 10-11 0.19661500954476452 1105 12-13 0.4155296331837448 1105 14-15 0.38758068934654233 1105 16-17 0.23346448308245016 1105 18-19 0.16077084796676644 1105 20-21 0.16534504289368357 1105 22-23 0.05715262764643825 1105 24-25 0.2533301060096349 1105 26-27 0.2564959463424472 1105 28-29 0.2398267125155371 1105 30-31 0.31289527260010885 1105 32-33 0.37778587910502637 1105 34-35 0.5504088161969136 1105 36-37 0.2836795532922096 1105 38-39 0.40626812241234234 1105 40-41 0.45746130121651163 1105 42-43 0.5010726037027879 1105 44-45 0.5055938554365582 1105 46-47 0.6661661583952565 1105 48-49 0.8660736403095868 1105 50-51 0.6093265873072866 1105 52-53 0.5705580075821146 1105 54-55 0.6802711750222272 1105 56-57 0.9097010455403165 1105 58-59 0.9288551223829558 1105 60-61 0.8756820324302268 1105 62-63 0.9125646173750752 1105 64-65 0.9459488544870496 1105 66-67 1.0613041243556864 1105 68-69 0.951644106465416 1105 70-71 1.0255384322433798 1105 72-73 1.054084251881715 1105 74-75 0.9283385048192621 1105 76-77 0.9826240221588591 1105 78-79 1.0354543860123755 1105 80-81 0.9003112865711635 1105 82-83 1.037012301247895 1105 84-85 0.9129599491287124 1105 86-87 0.85915030898013 1105 88-89 1.0299636857928967 1105 90-91 1.1281856445543887 1105 92-93 1.3332849930929669 1105 94-95 1.2370966209445449 1105 96-97 1.2520888926036768 1105 98-99 1.3538321361258845 1105 100-101 1.5346298914143865 1106 1 0.12178083365526504 1106 2 -0.04208058170852169 1106 3 -0.06224551830117875 1106 4 -0.18644524017942388 1106 5 -0.22996897793468918 1106 6 2.0004938548368045E-4 1106 7 -0.1782483191176354 1106 8 -0.10582380490432541 1106 9 -0.05931012097329358 1106 10-11 -0.4915496407239175 1106 12-13 -0.52701106248211 1106 14-15 -0.4250786642431237 1106 16-17 -0.5133744410948324 1106 18-19 -0.21980332093178845 1106 20-21 -0.29823802278376377 1106 22-23 -0.04917807530512874 1106 24-25 0.05391364713971569 1106 26-27 -0.08799786764270578 1106 28-29 -0.07985292135174404 1106 30-31 -0.09058168847091963 1106 32-33 0.1234064131853998 1106 34-35 0.07573231210767517 1106 36-37 0.05744004146917092 1106 38-39 0.0030819057496032087 1106 40-41 0.0353838811113647 1106 42-43 -0.18734790164519666 1106 44-45 -0.10890950180622383 1106 46-47 0.1243225652048281 1106 48-49 0.09769910678333815 1106 50-51 0.1668235173883872 1106 52-53 0.0036163309104111363 1106 54-55 0.04631359634165477 1106 56-57 0.08711670400798965 1106 58-59 0.13479691193189325 1106 60-61 -0.0687071135679318 1106 62-63 -0.09342129691346912 1106 64-65 0.061179756855075595 1106 66-67 0.2701266779125149 1106 68-69 -0.1247994395217944 1106 70-71 0.016535617153408566 1106 72-73 0.053759986353401956 1106 74-75 -0.09586305972770859 1106 76-77 0.023813269404481474 1106 78-79 0.2698980209331694 1106 80-81 0.1436010766536988 1106 82-83 0.0768521523867598 1106 84-85 0.1945052043509925 1106 86-87 0.13319299025927123 1106 88-89 0.2331789768964576 1106 90-91 0.3519965854375009 1106 92-93 0.3696318067052786 1106 94-95 0.47879005319938983 1106 96-97 0.6107361443849122 1106 98-99 0.8625243677773504 1106 100-101 0.9343648009244596 1107 1 0.00574932336251166 1107 2 0.006162464735034234 1107 3 -0.016387372294516922 1107 4 1.489470463127418E-4 1107 5 0.006366869108703099 1107 6 0.18986740335719077 1107 7 0.14385262340347538 1107 8 0.0026992582652596298 1107 9 0.08311005791827597 1107 10-11 0.1721779557751475 1107 12-13 0.13500692280090476 1107 14-15 0.1562141421242984 1107 16-17 0.3978661204766496 1107 18-19 0.0958355122837915 1107 20-21 -0.18756172423383788 1107 22-23 2.2521895274252302E-4 1107 24-25 0.15469034990190522 1107 26-27 0.07574366721039638 1107 28-29 -0.001825054256350711 1107 30-31 -0.026729127418526843 1107 32-33 -0.08991384693312199 1107 34-35 -0.07098424425374361 1107 36-37 -0.1078332704279319 1107 38-39 0.053839623512359935 1107 40-41 0.08602961140902465 1107 42-43 0.0999931891030954 1107 44-45 0.26687669345569986 1107 46-47 0.34131534129238617 1107 48-49 0.5228708128859338 1107 50-51 0.3114353822920961 1107 52-53 0.1376014024526242 1107 54-55 0.2844441266239315 1107 56-57 0.16870413834151776 1107 58-59 0.17279767133187107 1107 60-61 0.1911134181081806 1107 62-63 0.2830127914941514 1107 64-65 0.4651286367400971 1107 66-67 0.8506245174676437 1107 68-69 0.7029201015699087 1107 70-71 0.7898907712202785 1107 72-73 0.568418809091181 1107 74-75 0.6290284373957071 1107 76-77 0.7094654161513212 1107 78-79 0.9299901101963144 1107 80-81 0.9273162728240152 1107 82-83 0.7791558258639952 1107 84-85 0.9568980832939893 1107 86-87 1.154361985592324 1107 88-89 0.9295040577629443 1107 90-91 1.0480928850448237 1107 92-93 1.391753897073876 1107 94-95 1.4898282683210624 1107 96-97 1.2780478203998875 1107 98-99 1.3580948549949134 1107 100-101 1.4847538732549062 1108 1 -0.04453675803790347 1108 2 -0.053378723671592354 1108 3 0.003197833528247429 1108 4 0.18311382534642462 1108 5 -0.08795889157522652 1108 6 -0.062278604702065365 1108 7 -0.06886853707880647 1108 8 0.030534549165039948 1108 9 -0.07435715960218658 1108 10-11 0.030215427826952634 1108 12-13 0.09834588552921986 1108 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-1.650685737112351 2316 74-75 -1.871615556454607 2316 76-77 -1.738832913243975 2316 78-79 -1.6458976111387784 2316 80-81 -1.7391175885540413 2316 82-83 -1.8216798544187611 2316 84-85 -1.8139504617485436 2316 86-87 -2.028923804063563 2316 88-89 -1.8964762565846662 2316 90-91 -2.1518248772428343 2316 92-93 -2.2455838383958238 2316 94-95 -2.4093882058277245 2316 96-97 -2.5247252836353518 2316 98-99 -2.5791973776229185 2316 100-101 -3.265713626975632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4329.0 3 913.0 4 815.0 5 862.0 6 940.0 7 1070.0 8 1278.0 9 1614.0 10 2018.0 11 2744.0 12 3794.0 13 5014.0 14 5771.0 15 7516.0 16 9708.0 17 13372.0 18 11225.0 19 6802.0 20 6587.0 21 6604.0 22 6889.0 23 7608.0 24 8409.0 25 9357.0 26 10732.0 27 12499.0 28 14393.0 29 17456.0 30 21321.0 31 26652.0 32 33530.0 33 43903.0 34 61284.0 35 87727.0 36 133446.0 37 206121.0 38 193903.0 39 29773.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.674006395974715 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20.249367304169443 26.76138483360912 32.331126075533696 20.658121786687744 20-21 20.904470874498365 25.895065708202626 32.64581945033669 20.554643966962324 22-23 20.67237536241228 25.887568649781407 32.50841219742789 20.931643790378423 24-25 20.47767008255613 26.027375014271147 32.615084504879746 20.879870398292972 26-27 20.316974446672653 26.361289846335552 32.33045853274443 20.991277174247365 28-29 20.06352434607725 26.206869055974863 32.80284811154611 20.926758486401773 30-31 20.175688614206212 26.1645093246892 32.63225257022745 21.02754949087714 32-33 20.014737530198644 26.32027399795624 32.41341085713948 21.25157761470563 34-35 20.501282373153355 26.248824696390155 32.25618911188891 20.99370381856758 36-37 20.48754584306955 26.10604095018928 32.138477075020226 21.267936131720944 38-39 20.219085616028813 26.470884959628265 32.30806632509691 21.00196309924601 40-41 20.439953051989754 26.271594731389964 32.33723828575788 20.951213930862412 42-43 20.215585401181084 26.37456363937923 31.988377169130004 21.42147379030968 44-45 20.35807043974763 26.593098954167754 31.717918139718936 21.33091246636568 46-47 20.477414204098828 26.802608516316518 31.167477910844028 21.552499368740627 48-49 20.620445501568295 26.989015056795246 30.856733027878796 21.53380641375766 50-51 20.702358917811996 27.10683062261253 30.644616626629386 21.546193832946088 52-53 20.957209946301944 27.28906058436641 30.300258101237524 21.453471368094117 54-55 20.57084322534692 27.192285976861676 30.629759672678748 21.607111125112663 56-57 20.628521853638517 27.526003465324365 30.354361648573196 21.491113032463918 58-59 20.74622231167411 27.027713299697016 30.607308886394513 21.618755502234357 60-61 20.78082673746005 27.010857664458065 30.821004770680123 21.387310827401762 62-63 20.61805798199812 26.94607802126958 31.03030989020723 21.405554106525067 64-65 20.532636155800084 26.87489481811284 30.824271698469737 21.768197327617344 66-67 20.368974347366535 27.21161208289621 30.539781953599693 21.87963161613756 68-69 20.41364915253737 26.96429959720553 30.868985022895217 21.753066227361877 70-71 20.481754583499008 27.009771307315884 30.673027333677155 21.835446775507954 72-73 20.570988489683035 26.920953304233574 30.49800943855833 22.010048767525063 74-75 20.40834906916235 26.99293843907466 30.449555041444654 22.14915745031833 76-77 20.437431377595843 26.908594834624623 30.575805641173787 22.078168146605755 78-79 20.338275727838088 26.883614311143 30.440780094202246 22.337329866816667 80-81 20.3591052143337 27.135056926813306 30.333982121018998 22.171855737834 82-83 20.509003620344597 27.084478479937523 30.362570843259306 22.043947056458578 84-85 20.389804654824584 26.760960488116915 30.214584460955567 22.634650396102938 86-87 20.39763196211454 26.74100532862652 30.501453820948505 22.359908888310436 88-89 20.411572651381135 26.423662967072715 30.424095994252546 22.740668387293606 90-91 20.28545825232112 26.416551145833793 30.939062576121955 22.35892802572313 92-93 20.391515041767377 26.210112350704257 30.79822537850456 22.6001472290238 94-95 19.97394006017012 26.20170472159265 30.78000706610151 23.044348152135726 96-97 19.885160862381003 26.23664400851477 30.852106597960667 23.02608853114356 98-99 19.863554022538242 26.692152067924763 30.55881040131141 22.885483508225587 100-101 19.69330919039137 26.890382514736704 30.610527766023406 22.80578052884852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1537.0 1 914.0 2 340.5 3 395.0 4 304.0 5 258.5 6 527.0 7 1191.5 8 2088.5 9 2550.0 10 2270.5 11 1896.5 12 1925.5 13 2050.0 14 2140.5 15 2241.5 16 2302.0 17 2392.5 18 2531.0 19 2736.5 20 2964.5 21 3306.5 22 3792.0 23 4368.5 24 5096.0 25 6073.0 26 7613.0 27 9376.5 28 10545.0 29 12093.5 30 14383.0 31 16543.0 32 18893.5 33 21798.5 34 25230.5 35 27878.0 36 30621.0 37 33177.5 38 34479.5 39 35065.5 40 34854.0 41 35473.0 42 36036.5 43 36809.0 44 37797.0 45 38105.5 46 37268.0 47 36338.5 48 37670.5 49 37332.0 50 34815.0 51 32517.5 52 30184.5 53 28746.5 54 27192.0 55 24189.5 56 20864.0 57 18023.0 58 15819.0 59 13778.0 60 11682.0 61 9457.0 62 7249.5 63 5429.0 64 3967.5 65 3024.5 66 2194.0 67 1640.5 68 1249.5 69 820.0 70 588.5 71 445.0 72 339.0 73 272.5 74 208.0 75 147.5 76 97.5 77 68.5 78 43.0 79 19.5 80 17.0 81 14.0 82 8.0 83 5.5 84 4.5 85 4.5 86 5.5 87 4.5 88 4.0 89 4.0 90 2.5 91 3.5 92 3.5 93 1.5 94 1.0 95 2.5 96 5.5 97 6.5 98 4.0 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3534429746578047 2 0.2995129599031814 3 0.29293133697320006 4 0.2978429958761712 5 0.2999058926154191 6 0.22318578055100954 7 0.2094331356226903 8 0.2109066332935816 9 0.20491440943195682 10-11 0.2286377219333075 12-13 0.21778295575774126 14-15 0.21021900104716568 16-17 0.2133133461560375 18-19 0.20579850803449165 20-21 0.20186918091211473 22-23 0.2014271316108473 24-25 0.19155469721587529 26-27 0.19386317690027172 28-29 0.21169249871805704 30-31 0.22284196442780158 32-33 0.21729178986744416 34-35 0.22505221093413857 36-37 0.1984310196800349 38-39 0.1955331409272819 40-41 0.19337201100997461 42-43 0.201820064323085 44-45 0.20594585780158076 46-47 0.21213454801932444 48-49 0.21925645342863262 50-51 0.222596381482653 52-53 0.24489531290214206 54-55 0.21861793777124636 56-57 0.18855858528506286 58-59 0.18875505164118173 60-61 0.1850221908749236 62-63 0.19459992573571738 64-65 0.18556247335425044 66-67 0.18389250932724024 68-69 0.1802578817390416 70-71 0.19155469721587529 72-73 0.19067059861334046 74-75 0.19572960728340075 76-77 0.19739957131041092 78-79 0.19455080914668768 80-81 0.19533667457116308 82-83 0.18885328481924113 84-85 0.18035611491710102 86-87 0.1837451595601511 88-89 0.1850221908749236 90-91 0.19013031613401363 92-93 0.18487484110783448 94-95 0.18340134343694314 96-97 0.18359780979306198 98-99 0.19445257596862825 100-101 0.17839145135591256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1017986.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.00145261132942 #Duplication Level Percentage of deduplicated Percentage of total 1 81.60188782314914 48.146299173925534 2 10.771591422820823 12.710790817643305 3 3.092404065653097 5.473689958041409 4 1.338570890362823 3.159105078185885 5 0.7677051798314694 2.264786039364928 6 0.4475026835099282 1.5841985024732268 7 0.332549792006977 1.3734644555804973 8 0.2231122820936812 1.0531158991164715 9 0.17091274599530024 0.90756902551625 >10 1.104473141879677 12.51620859528359 >50 0.10269821309801877 4.1220101325152845 >100 0.04542696577983871 4.5354265252812604 >500 1.663991170418752E-4 0.05527415555335564 >1k 6.655964681675008E-4 0.7663766576367569 >5k 3.327982340837504E-4 1.3316849838822662 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7777 0.7639594257681344 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5787 0.5684754014298821 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3881 0.38124296404862146 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1469 0.1443045385692927 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1374 0.1349723866536475 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1082 0.1062882986602959 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.9116589029711605E-5 0.0 12-13 0.0 0.0 0.0 9.823317805942321E-5 0.0 14-15 0.0 0.0 0.0 9.823317805942321E-5 0.0 16-17 0.0 0.0 0.0 9.823317805942321E-5 0.0 18-19 0.0 0.0 0.0 1.4734976708913482E-4 0.0 20-21 0.0 0.0 0.0 2.4558294514855805E-4 0.0 22-23 0.0 0.0 0.0 7.858654244753857E-4 0.0 24-25 0.0 0.0 0.0 0.0010805649586536554 0.0 26-27 0.0 0.0 0.0 0.0015226142599210598 0.0 28-29 0.0 0.0 0.0 0.0027505289856638504 0.0 30-31 0.0 0.0 0.0 0.006237806806773374 0.0 32-33 0.0 0.0 0.0 0.009823317805942321 0.0 34-35 0.0 0.0 0.0 0.01252473020257646 0.0 36-37 0.0 0.0 0.0 0.01689610662622079 0.0 38-39 0.0 0.0 0.0 0.02288833048784561 0.0 40-41 0.0 0.0 0.0 0.03163108333513427 0.0 42-43 0.0 0.0 0.0 0.04754485818076083 0.0 44-45 0.0 0.0 0.0 0.06719149379264548 0.0 46-47 0.0 0.0 0.0 0.08782046118512435 0.0 48-49 0.0 0.0 0.0 0.11449076902825775 0.0 50-51 0.0 0.0 0.0 0.14833209886972906 0.0 52-53 0.0 0.0 0.0 0.21242924755350268 0.0 54-55 0.0 0.0 0.0 0.25756739287180763 0.0 56-57 0.0 0.0 0.0 0.347254284440061 0.0 58-59 0.0 0.0 0.0 0.4578648429349716 0.0 60-61 0.0 0.0 0.0 0.574123809168299 0.0 62-63 0.0 0.0 0.0 0.7569357535368856 0.0 64-65 0.0 0.0 0.0 0.8661219309499344 0.0 66-67 0.0 0.0 0.0 1.1026674237170255 0.0 68-69 0.0 0.0 0.0 1.3581228032605557 0.0 70-71 0.0 0.0 0.0 1.5689312033760778 0.0 72-73 0.0 0.0 0.0 1.9348989082364592 0.0 74-75 0.0 0.0 0.0 2.284952838251214 0.0 76-77 0.0 0.0 0.0 2.5337774782757325 0.0 78-79 0.0 0.0 0.0 2.821797156345961 0.0 80-81 0.0 0.0 0.0 3.3193973198059696 0.0 82-83 0.0 0.0 0.0 3.7290296723137644 0.0 84-85 0.0 0.0 0.0 4.321965135080443 0.0 86-87 0.0 0.0 0.0 5.028359918505755 0.0 88-89 0.0 0.0 0.0 6.02208674775488 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2685 0.0 50.66327 1 GTATCAA 5050 0.0 43.607487 1 ATCAACG 6510 0.0 33.22839 3 TCAACGC 6580 0.0 33.017776 4 TATCAAC 6610 0.0 32.87278 2 CAACGCA 6645 0.0 32.55172 5 AACGCAG 6795 0.0 31.735043 6 ACGCAGA 7470 0.0 28.48591 7 CGCAGAG 7585 0.0 28.054022 8 GCAGAGT 7915 0.0 26.52431 9 GTACATG 4165 0.0 26.15122 1 TACATGG 4295 0.0 24.686718 2 ACATGGG 4205 0.0 24.42118 3 GTGGTAT 1560 0.0 22.866943 1 GAGTACT 5385 0.0 22.800667 12-13 AGAGTAC 7410 0.0 22.595064 10-11 TGGTATC 1405 0.0 21.99676 2 AGTACTT 5630 0.0 21.850636 12-13 CAGAGTA 7855 0.0 21.466187 10-11 GTACTTT 5740 0.0 21.266397 14-15 >>END_MODULE