FastQCFastQC Report
Thu 26 May 2016
SRR937450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017986
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51740.5082584632794558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41910.41169524924704265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.28418858412591136No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.10520773370164226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.044.59791
GTATCAA54150.034.8480031
GACGCGT650.005839983529.2346388
TTGCGTA2150.028.71908860-61
ATCAACG67600.027.5480253
TCAACGC68100.027.3457624
TATCAAC68500.027.3247852
CAACGCA69000.026.989085
AACGCAG70750.026.3834676
GCGTAAG2350.026.27491262-63
TGCGTAA2400.024.73799760-61
CGTAAGT2550.024.21413262-63
GTATAGG3000.023.7659851
GTACATG48700.023.0339761
ACGCAGA81800.022.6496987
TATAGGG2103.4404366E-822.6220422
GAGTACT45500.022.5002312-13
CGCAGAG81900.022.3900188
GTACTTT47500.021.49862314-15
TAATTCG1551.0913936E-1121.45023758-59
Produced by FastQC (version 0.11.5)