##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937450_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1017986 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22643631641299 33.0 31.0 34.0 31.0 34.0 2 32.44960932665086 34.0 31.0 34.0 31.0 34.0 3 32.52727640655176 34.0 31.0 34.0 31.0 34.0 4 36.13283385036729 37.0 35.0 37.0 35.0 37.0 5 35.99991846646221 37.0 35.0 37.0 35.0 37.0 6 36.09034014220235 37.0 35.0 37.0 35.0 37.0 7 36.050068468525104 37.0 35.0 37.0 35.0 37.0 8 36.02844931069779 37.0 35.0 37.0 35.0 37.0 9 37.7266789523628 39.0 37.0 39.0 35.0 39.0 10-11 37.654663227195655 39.0 37.0 39.0 35.0 39.0 12-13 37.57282467538846 39.0 37.0 39.0 35.0 39.0 14-15 38.799624945726165 40.0 38.0 41.0 35.0 41.0 16-17 38.77795028615324 40.0 38.0 41.0 35.0 41.0 18-19 38.79106392425829 40.0 38.0 41.0 35.0 41.0 20-21 38.715092349010696 40.0 38.0 41.0 34.0 41.0 22-23 38.583744275461555 40.0 38.0 41.0 34.0 41.0 24-25 38.44111706840762 40.0 38.0 41.0 34.0 41.0 26-27 38.234777295562026 40.0 38.0 41.0 34.0 41.0 28-29 38.22113958345203 40.0 38.0 41.0 34.0 41.0 30-31 38.345327440652426 40.0 38.0 41.0 34.0 41.0 32-33 38.312715007868476 40.0 38.0 41.0 34.0 41.0 34-35 38.21410608790298 40.0 38.0 41.0 34.0 41.0 36-37 38.06704856451857 40.0 37.5 41.0 33.5 41.0 38-39 37.92730793940191 40.0 37.0 41.0 33.0 41.0 40-41 37.77639034328567 40.0 37.0 41.0 33.0 41.0 42-43 37.57117337566528 40.0 37.0 41.0 33.0 41.0 44-45 37.27571155202527 40.0 37.0 41.0 32.5 41.0 46-47 36.92935610116446 40.0 37.0 41.0 31.5 41.0 48-49 36.609000025540624 40.0 36.0 41.0 31.0 41.0 50-51 36.39101470943608 39.5 36.0 41.0 30.0 41.0 52-53 36.1711079523687 39.0 36.0 41.0 30.0 41.0 54-55 35.970330142064824 39.0 35.0 41.0 29.0 41.0 56-57 35.74167719398892 39.0 35.0 40.0 28.5 41.0 58-59 35.50630951702676 38.5 35.0 40.0 28.0 41.0 60-61 35.187067405642125 38.0 35.0 40.0 28.0 41.0 62-63 34.82554868141605 38.0 34.0 40.0 27.0 41.0 64-65 34.490033752919985 37.5 34.0 40.0 26.0 41.0 66-67 34.11367248665502 37.0 34.0 39.5 26.0 41.0 68-69 33.684183279534295 36.0 33.0 39.0 26.0 40.5 70-71 33.30578907764939 36.0 33.0 39.0 25.0 40.0 72-73 32.86602664476722 35.0 33.0 38.0 24.0 40.0 74-75 32.426316766635296 35.0 32.0 37.5 22.5 39.5 76-77 31.106417475289447 34.0 30.5 36.0 21.5 39.0 78-79 31.696777264127405 35.0 32.0 36.0 22.0 39.0 80-81 31.511032568227854 35.0 32.0 36.0 21.0 38.5 82-83 31.187607688121446 35.0 31.5 36.0 20.0 37.0 84-85 30.855707249412074 34.0 31.0 35.0 19.5 37.0 86-87 30.52921405598898 34.0 31.0 35.0 18.0 36.0 88-89 30.189646517732072 34.0 31.0 35.0 15.0 36.0 90-91 29.779383017055245 34.0 30.5 35.0 7.5 36.0 92-93 29.393011298780138 34.0 30.0 35.0 2.0 35.0 94-95 29.019393685178382 34.0 29.5 35.0 2.0 35.0 96-97 28.63823569282878 34.0 29.0 35.0 2.0 35.0 98-99 28.04727569927288 34.0 29.0 35.0 2.0 35.0 100-101 26.477923566728816 32.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11060618657479893 1101 2 0.15607590679876182 1101 3 0.18023291957121046 1101 4 0.17258471571127387 1101 5 0.06739437910715651 1101 6 0.0915136069605964 1101 7 0.11046120391340963 1101 8 0.10398015977755648 1101 9 0.19450071309790218 1101 10-11 0.1350571527528288 1101 12-13 0.16713439967969634 1101 14-15 0.20484274171877104 1101 16-17 0.30553661220356787 1101 18-19 0.2693772517806323 1101 20-21 0.24572284271583555 1101 22-23 0.09396653605302419 1101 24-25 0.23180415760735684 1101 26-27 0.2023337608884077 1101 28-29 0.1531537602003752 1101 30-31 0.12315951716514206 1101 32-33 0.21477004840827618 1101 34-35 0.3154478186886607 1101 36-37 0.22701671543113378 1101 38-39 0.1825221553943308 1101 40-41 0.12141999708236995 1101 42-43 0.32555828489882543 1101 44-45 0.3457078336885502 1101 46-47 0.3043020238250378 1101 48-49 0.2247195662685897 1101 50-51 0.16669615506596358 1101 52-53 0.3078181776071318 1101 54-55 0.15919019106850385 1101 56-57 0.14968199944583205 1101 58-59 0.33205668069671646 1101 60-61 0.22127499256707495 1101 62-63 0.23994412201241033 1101 64-65 0.2391545488369644 1101 66-67 0.18184254680576828 1101 68-69 0.0382157896082731 1101 70-71 -0.029309754382445874 1101 72-73 -0.021399468139577493 1101 74-75 0.009529748879039346 1101 76-77 0.02824639144094121 1101 78-79 0.13181877806365705 1101 80-81 0.004625039990390434 1101 82-83 0.02692872390055001 1101 84-85 0.12015962086460519 1101 86-87 -0.08099620773053573 1101 88-89 0.0703603884655486 1101 90-91 0.28122461975463864 1101 92-93 0.29548357854369556 1101 94-95 0.0442882260108135 1101 96-97 -0.17494875983484448 1101 98-99 -0.1036286762803762 1101 100-101 0.17901175710238348 1102 1 0.08616876181200439 1102 2 0.11491849359779849 1102 3 -0.030328035577412038 1102 4 0.014037663408750234 1102 5 -0.030366275029415135 1102 6 -0.02315116531899264 1102 7 0.04853547686441573 1102 8 -0.023094196179293647 1102 9 0.05864712023016949 1102 10-11 0.06702670435696945 1102 12-13 0.03811762280908226 1102 14-15 0.09220002539692018 1102 16-17 0.030773168312364874 1102 18-19 0.05520238249375353 1102 20-21 0.04160179239281092 1102 22-23 0.09657953459894486 1102 24-25 0.10130549475979933 1102 26-27 0.0486111596623644 1102 28-29 -0.04589824569683287 1102 30-31 -0.08876724218998078 1102 32-33 -0.109009818420013 1102 34-35 -0.08280429743356166 1102 36-37 -0.09574496047783043 1102 38-39 -0.22951588234762 1102 40-41 -0.18467751918907993 1102 42-43 -0.106970397410862 1102 44-45 -0.04989146636576436 1102 46-47 -0.21476951583215964 1102 48-49 0.0784026027823117 1102 50-51 0.1755425972123703 1102 52-53 0.1908311378945271 1102 54-55 0.0771745227682672 1102 56-57 0.09883038902720642 1102 58-59 0.04048139418963359 1102 60-61 0.01007632504254019 1102 62-63 0.05911948217138274 1102 64-65 0.10158669577668888 1102 66-67 0.1932646980727455 1102 68-69 0.13056547424928766 1102 70-71 -0.07197496655084734 1102 72-73 -0.03803497601522565 1102 74-75 -0.21745498605212532 1102 76-77 -0.16254927219917548 1102 78-79 -0.13329934514482034 1102 80-81 0.07834981537953567 1102 82-83 0.2721243922597729 1102 84-85 -0.016953174801560777 1102 86-87 0.09078795854743404 1102 88-89 0.13027059047310985 1102 90-91 0.17617188155210783 1102 92-93 0.10495505044790221 1102 94-95 0.1618685257335244 1102 96-97 0.15298034559394935 1102 98-99 0.11148643427961247 1102 100-101 0.18578157853870536 1103 1 0.01389133429663758 1103 2 0.02582748607248675 1103 3 0.06238486572733848 1103 4 0.042010751443555705 1103 5 -0.010905301808499246 1103 6 -0.006892263157865841 1103 7 -0.09273609872581545 1103 8 -0.03532663917347634 1103 9 -0.07960694880187447 1103 10-11 0.014534345327341214 1103 12-13 0.04097788041190853 1103 14-15 0.02489919510274774 1103 16-17 -0.029992848195092847 1103 18-19 -0.028048758114863404 1103 20-21 -0.10283740221692028 1103 22-23 -0.026907651702920532 1103 24-25 -0.08741195421426085 1103 26-27 -0.1306824258599022 1103 28-29 -0.07319459722577193 1103 30-31 0.007749164363779926 1103 32-33 0.05581243366064115 1103 34-35 0.16690630542793627 1103 36-37 0.19702084931737573 1103 38-39 0.16127656379645572 1103 40-41 0.06926133336110496 1103 42-43 0.0410306459937857 1103 44-45 -0.07031291325301936 1103 46-47 -0.17799590018896794 1103 48-49 -0.2957367114252136 1103 50-51 -0.2611881994664529 1103 52-53 -0.1354461714995594 1103 54-55 -0.10319273598131673 1103 56-57 -0.1042296904093547 1103 58-59 -0.2089624514455366 1103 60-61 -0.31842904701488806 1103 62-63 -0.061480130981422576 1103 64-65 -0.1850183509550476 1103 66-67 -0.21505768443348927 1103 68-69 -0.2168210924080327 1103 70-71 -0.3581481323488376 1103 72-73 -0.18627176980992033 1103 74-75 -0.2604459645392936 1103 76-77 -0.4502651511445457 1103 78-79 -0.17830084626943332 1103 80-81 -0.13642961773674855 1103 82-83 -0.19157300070511596 1103 84-85 -0.2045248473496848 1103 86-87 -0.13085087579238674 1103 88-89 -0.08898736179604683 1103 90-91 -0.004851934651178169 1103 92-93 -0.10582893033770091 1103 94-95 -0.30225804121909405 1103 96-97 -0.13990309331028072 1103 98-99 -0.2733182472604945 1103 100-101 -0.03618479626996063 1104 1 0.0738304013229083 1104 2 0.07366486495571678 1104 3 0.0529802108477071 1104 4 0.07060929304778796 1104 5 -0.04285515696781772 1104 6 -0.10932958210702282 1104 7 0.014890512002381229 1104 8 0.02771901061176152 1104 9 0.006268588107523954 1104 10-11 0.06788316162940333 1104 12-13 0.0750029528322429 1104 14-15 0.12366804936306153 1104 16-17 0.11337694503235696 1104 18-19 0.17797461617342236 1104 20-21 0.008824769394301768 1104 22-23 0.0037136648488669266 1104 24-25 -0.04510349352102594 1104 26-27 0.04535393414409583 1104 28-29 -0.15630817229319405 1104 30-31 -0.09994736944901916 1104 32-33 -0.03437585921719233 1104 34-35 -0.1549321722466246 1104 36-37 -0.01617213839614351 1104 38-39 -0.21023227647923903 1104 40-41 -0.16739834099710293 1104 42-43 -0.11689064059097376 1104 44-45 0.010917733036059474 1104 46-47 -0.10307331503263839 1104 48-49 -0.11290282869621393 1104 50-51 -0.08727200727786766 1104 52-53 0.09638381551472719 1104 54-55 -0.07827015082499855 1104 56-57 -0.11217844677734945 1104 58-59 -0.09789962055950951 1104 60-61 -0.1361195874204384 1104 62-63 -0.1424810299925099 1104 64-65 -0.08708607645699828 1104 66-67 0.002353163633635802 1104 68-69 -0.09746987874306257 1104 70-71 -0.06960303195925377 1104 72-73 -0.08705341000570854 1104 74-75 -0.18368540115901055 1104 76-77 -0.20293471462472112 1104 78-79 -0.23821743801850914 1104 80-81 0.04379463558460017 1104 82-83 0.0014047237979291083 1104 84-85 -0.2952925029285467 1104 86-87 -0.16942844047123984 1104 88-89 -0.20437793216863653 1104 90-91 -0.2391192406146203 1104 92-93 -0.1856586176816215 1104 94-95 -0.4756323295019804 1104 96-97 -0.3321691007021492 1104 98-99 -0.17174997236287837 1104 100-101 0.3981299575008883 1105 1 0.006016884063747341 1105 2 -0.018183095422877216 1105 3 0.011286954436620533 1105 4 0.05403340752872765 1105 5 -0.07364285800240111 1105 6 -0.011801622587896077 1105 7 -0.031877894009589625 1105 8 -0.05301980826641994 1105 9 -0.037683453662552324 1105 10-11 -0.008103076163486378 1105 12-13 -0.09382339196422151 1105 14-15 -0.030625254318678685 1105 16-17 0.04129429658638628 1105 18-19 0.046530497610014265 1105 20-21 -0.055657240897808435 1105 22-23 -0.06872646793870274 1105 24-25 -0.015384753928628925 1105 26-27 0.03883004634744225 1105 28-29 0.002023280957196505 1105 30-31 -0.060902344148480836 1105 32-33 -0.07319822898116257 1105 34-35 0.034797471826813364 1105 36-37 -0.029014984949142786 1105 38-39 -0.10956914436849985 1105 40-41 4.678185843403071E-4 1105 42-43 -0.20118335338950288 1105 44-45 -0.11462292510040584 1105 46-47 -0.05790566535117847 1105 48-49 0.08414221809098166 1105 50-51 0.0708760035943854 1105 52-53 0.10184142770195592 1105 54-55 0.32040627078628603 1105 56-57 0.18512470853899288 1105 58-59 0.056658237650005105 1105 60-61 0.07206951669317618 1105 62-63 0.16396658015349175 1105 64-65 0.2926079534739259 1105 66-67 0.21552072736844252 1105 68-69 0.30824423047364036 1105 70-71 0.26605703830212946 1105 72-73 0.3160457724268042 1105 74-75 0.1929865297398905 1105 76-77 0.28228917639492934 1105 78-79 0.4017200895112758 1105 80-81 0.31861038745662285 1105 82-83 0.19844923341858234 1105 84-85 0.20231092849139642 1105 86-87 0.27420130136651366 1105 88-89 0.326772087706523 1105 90-91 0.15909145759446375 1105 92-93 0.36014799161565847 1105 94-95 0.21970667631016028 1105 96-97 0.3656465115065721 1105 98-99 0.39796565166350817 1105 100-101 0.3252974245457203 1106 1 -0.1980795336230905 1106 2 -0.05929161833866914 1106 3 -0.07307612718046386 1106 4 -0.05215329142767189 1106 5 -0.19173092277341652 1106 6 -0.2736193566285934 1106 7 -0.15235827218254627 1106 8 -0.10930176877306508 1106 9 -0.28438384668057637 1106 10-11 -0.2112790312588615 1106 12-13 -0.45167708253692496 1106 14-15 -0.18714323087441898 1106 16-17 -0.11475575249811243 1106 18-19 -0.22048745671232695 1106 20-21 -0.38492562535084573 1106 22-23 -0.2694878762362052 1106 24-25 -0.3069647958642321 1106 26-27 -0.09517849811764023 1106 28-29 -0.24213840029799627 1106 30-31 -0.15287164153895816 1106 32-33 -0.1890417469200898 1106 34-35 -0.34671357817096293 1106 36-37 -0.30284080510490696 1106 38-39 -0.3936514010923915 1106 40-41 -0.5204931429929331 1106 42-43 -0.7393974302075961 1106 44-45 -0.5227808711450805 1106 46-47 -0.43405607256437406 1106 48-49 -0.5584374715308016 1106 50-51 -0.6128467562199447 1106 52-53 -0.6108794952918188 1106 54-55 -0.4794322805540432 1106 56-57 -0.4598589242610842 1106 58-59 -0.4884888961232079 1106 60-61 -0.4791404931237224 1106 62-63 -0.4606929923496139 1106 64-65 -0.48042909420473734 1106 66-67 -0.3909235369284829 1106 68-69 -0.27253619592868716 1106 70-71 -0.22016988599829546 1106 72-73 -0.26520189734227984 1106 74-75 -0.3418360990684377 1106 76-77 -0.49728038505988437 1106 78-79 -0.5173985786741007 1106 80-81 -0.49234349363917573 1106 82-83 -0.698153161631236 1106 84-85 -0.5825453924630644 1106 86-87 -0.6456363606379831 1106 88-89 -0.5224304174841201 1106 90-91 -0.5775358388587932 1106 92-93 -0.3647387035781655 1106 94-95 -0.3765639280619517 1106 96-97 -0.23853712950275607 1106 98-99 -0.12192366022999224 1106 100-101 -0.13972294466547552 1107 1 -0.14475042985925057 1107 2 -0.07365485184455167 1107 3 -0.03470528044948651 1107 4 -0.03590309316660267 1107 5 0.03613809514938282 1107 6 0.009173505011617067 1107 7 0.03954594729365368 1107 8 -0.09859999667957453 1107 9 -0.03683483414086908 1107 10-11 -0.10135924888944459 1107 12-13 0.01112337310306799 1107 14-15 -0.009301849343216873 1107 16-17 -0.1571735282251936 1107 18-19 -0.13537698324085312 1107 20-21 -0.07392586086096742 1107 22-23 -0.09352528870093835 1107 24-25 -0.1647123578538583 1107 26-27 0.023340287419152617 1107 28-29 -0.15174240685382046 1107 30-31 -0.18119293501821687 1107 32-33 -0.2573560231590406 1107 34-35 -0.3308349091395826 1107 36-37 -0.20521513161806126 1107 38-39 -0.2491952612474435 1107 40-41 -0.3171734115705931 1107 42-43 -0.29033009834500234 1107 44-45 -0.02050219057851166 1107 46-47 -0.23823854003754263 1107 48-49 0.017585675749536733 1107 50-51 0.05254238820543122 1107 52-53 -0.05486990185919183 1107 54-55 -0.19001629758766825 1107 56-57 0.025674986792992627 1107 58-59 0.024071002751632875 1107 60-61 -0.05637002897653076 1107 62-63 0.10204065531763007 1107 64-65 -0.061915114996914156 1107 66-67 -0.07953397915881055 1107 68-69 0.037160572776350875 1107 70-71 -0.30853808924833004 1107 72-73 -0.013609800869645028 1107 74-75 0.17883592184870878 1107 76-77 0.08175008225947877 1107 78-79 0.19810904176048538 1107 80-81 0.2694014921581207 1107 82-83 0.3164085732612669 1107 84-85 0.31756130804015825 1107 86-87 0.18706205663788822 1107 88-89 0.19082495719325365 1107 90-91 0.3195443162895053 1107 92-93 0.3064262063752601 1107 94-95 0.26508819945956574 1107 96-97 0.19412235650842646 1107 98-99 0.03222780642129308 1107 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-0.4456866814750988 2316 100-101 -0.3916985055198303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 8.0 5 20.0 6 28.0 7 104.0 8 256.0 9 461.0 10 706.0 11 1234.0 12 1903.0 13 2790.0 14 3630.0 15 5093.0 16 7059.0 17 10600.0 18 9403.0 19 5460.0 20 4324.0 21 4608.0 22 4557.0 23 5135.0 24 5603.0 25 6758.0 26 8286.0 27 10134.0 28 12681.0 29 15467.0 30 19541.0 31 25207.0 32 32285.0 33 42796.0 34 60383.0 35 88244.0 36 140271.0 37 235029.0 38 222631.0 39 25289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.585805812475854 20.811969717965876 15.190550181277462 21.411674288280796 2 15.630372126925124 21.788806525826484 39.71095869687795 22.869862650370436 3 18.925112919038177 26.33818146811449 30.283618831693165 24.453086781154163 4 12.857065673855706 18.018438385634365 37.732186623575004 31.392309316934924 5 13.212416907209201 38.590031026548424 34.27549374261916 13.92205832362321 6 30.71957767592089 38.04030703762134 17.3819679249027 13.858147361555071 7 27.64880852978332 34.038189130302385 21.06384567174794 17.24915666816636 8 26.48553123520363 35.71208248443495 19.693591070997048 18.108795209364374 9 26.653018803794943 17.234421691457445 20.840758124374993 35.27180138037262 10-11 24.537321731340118 27.9908073391972 27.397675410074402 20.074195519388283 12-13 25.55069757892002 25.441749225253425 28.12847064116941 20.87908255465715 14-15 22.55807054320983 26.76780427235738 26.570060884923763 24.10406429950903 16-17 21.47627766983043 30.215002956818658 27.10852605045649 21.20019332289442 18-19 21.319448401058562 28.225879334293396 30.254885627110784 20.199786637537255 20-21 21.965577129744418 27.488246400245192 30.562650173970958 19.983526296039436 22-23 21.787976486583126 27.348689354913496 30.274506567960614 20.588827590542767 24-25 21.69105566467694 27.781011307202157 30.240785791855334 20.28714723626556 26-27 21.462676670309563 28.114886353868634 30.105283839041224 20.31715313678058 28-29 21.017052216611333 27.958817695954117 30.547864531222444 20.4762655562121 30-31 21.234134579727527 27.760807837901957 30.43410137660349 20.57095620576703 32-33 21.085702553866163 28.13403131280784 30.19461957237133 20.585646560954668 34-35 21.605061366266334 27.840363226999195 30.17119095940416 20.383384447330315 36-37 21.60160985164767 27.757219675789948 29.885400724177835 20.755769748384548 38-39 21.39939606004821 28.21764039049806 29.91526372764941 20.467699821804313 40-41 21.50020800885671 28.00923981726655 30.137020615716036 20.3535315581607 42-43 21.351764777543515 28.341120898510802 29.649304129636434 20.65781019430925 44-45 21.407416212010773 28.466697970306075 29.444609257887635 20.681276559795517 46-47 21.597693877911876 28.63737811718432 28.863216193542936 20.90171181136086 48-49 21.666811560675473 28.966178791348206 28.70419077678415 20.662818871192172 50-51 21.73144902358629 28.96558939199036 28.369117241846766 20.933844342576588 52-53 22.042225676767526 29.00599961197761 28.23319654316258 20.71857816809228 54-55 21.544991777882995 29.047845451705623 28.523231164279274 20.88393160613211 56-57 21.48128756191146 29.57309825478936 28.202696304271374 20.742917879027807 58-59 21.65908961419902 28.915181544736374 28.432905757053632 20.99282308401098 60-61 21.700642248518154 29.009878328385657 28.636199319047613 20.653280104048584 62-63 21.645238736092637 29.026823551600906 28.82682080107192 20.501116911234536 64-65 21.530011218228935 28.70560105934659 28.768814109427833 20.995573612996644 66-67 21.313947343087232 29.263565510724117 28.418858412591135 21.003628733597516 68-69 21.447200649124838 28.97181297188763 28.55412549877896 21.02686088020857 70-71 21.36036251972031 29.176727381319584 28.39263015404927 21.070279944910833 72-73 21.47991229741863 29.08905426990155 28.28801181941599 21.143021613263837 74-75 21.41660101415933 29.449275333845453 27.938449055291525 21.195674596703686 76-77 21.512623945712416 28.986498832007513 28.313601562300462 21.18727565997961 78-79 21.53197588179012 29.083602328519255 27.930344818101627 21.454076971589 80-81 21.29597067150236 29.59529895303079 27.90750560420281 21.201224771264044 82-83 21.464710837367047 29.209834157266947 28.105296505588257 21.220158499777746 84-85 21.105948955068612 29.309209217616555 27.819846156944276 21.764995670370553 86-87 21.05024037658671 29.145833046819895 28.18054472261898 21.623381853974415 88-89 21.181971068364398 28.88885505301645 28.156182894460237 21.77299098415892 90-91 21.054451625325974 28.881667073548723 28.278417354886788 21.78546394623852 92-93 20.98774709886312 29.06446501493669 28.098319879995636 21.849468006204546 94-95 20.826023147666078 28.81969889566261 28.207902662708523 22.146375293962787 96-97 20.752839430011807 29.254282475397503 27.994834899497633 21.998043195093057 98-99 20.624722180609005 29.755469818683704 27.583652800051084 22.036155200656207 100-101 20.340200280782142 30.001395068726783 27.642009645623073 22.016395004868002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 405.0 1 273.5 2 168.0 3 206.0 4 220.5 5 255.0 6 411.5 7 728.0 8 1108.5 9 1390.0 10 1447.0 11 1447.5 12 1592.0 13 1766.5 14 1910.5 15 2018.0 16 2103.0 17 2233.0 18 2324.0 19 2398.0 20 2616.0 21 2945.5 22 3383.5 23 4055.0 24 4734.0 25 5615.5 26 7170.5 27 8931.0 28 10261.0 29 12120.0 30 14514.5 31 16573.0 32 19033.5 33 22048.0 34 25559.5 35 27912.0 36 30959.0 37 34110.0 38 35357.5 39 35964.5 40 35413.5 41 35719.0 42 36485.0 43 37401.5 44 38693.0 45 38751.5 46 37275.5 47 36363.0 48 38190.5 49 38018.5 50 35666.0 51 33517.5 52 30691.0 53 28616.0 54 26883.5 55 24138.0 56 20733.0 57 17933.0 58 15849.5 59 13937.0 60 11956.5 61 9722.0 62 7526.5 63 5693.0 64 3984.5 65 2840.5 66 2147.5 67 1726.5 68 1204.5 69 681.0 70 499.0 71 404.0 72 348.0 73 285.0 74 201.0 75 143.5 76 102.0 77 75.0 78 42.0 79 20.0 80 14.5 81 12.5 82 8.0 83 4.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047643091358820264 2 0.0 3 0.0 4 9.823317805942321E-5 5 0.014538510352794635 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007121905409308183 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001375264492831925 24-25 6.385156573862509E-4 26-27 0.0023084796843964453 28-29 0.002406712862455869 30-31 9.332151915645206E-4 32-33 0.0 34-35 0.0 36-37 3.9293271223769284E-4 38-39 3.9293271223769284E-4 40-41 4.9116589029711605E-5 42-43 0.012180914079368478 44-45 0.0 46-47 0.0 48-49 4.9116589029711605E-5 50-51 4.9116589029711605E-5 52-53 3.4381612320798124E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.4381612320798124E-4 84-85 4.9116589029711605E-5 86-87 0.0 88-89 0.0 90-91 0.008104237189902415 92-93 0.002259363095366734 94-95 0.0 96-97 0.0 98-99 0.0013261479038022134 100-101 0.01129681547683367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1017986.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.09508765566452 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64459992503716 44.67670814095291 2 11.535774258098773 12.942005363680181 3 3.565382238769519 6.000012876291767 4 1.5902632035794586 3.5682381520147044 5 0.8533102132685445 2.3933255605386394 6 0.5581858539428743 1.8786890643046492 7 0.367928297652703 1.4447279075479462 8 0.26109780030971064 1.1717043196059527 9 0.21320546342257818 1.0763801243420488 >10 1.2379133423509132 13.073217608233776 >50 0.11337530102686136 4.422352249297945 >100 0.0564999312768347 5.391184688827298 >500 0.0017601223314690452 0.6454255730542492 >1k 5.280366994407136E-4 0.8051775353001991 >5k 1.7601223314690455E-4 0.5108508360077536 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5174 0.5082584632794558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4191 0.41169524924704265 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2893 0.28418858412591136 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1071 0.10520773370164226 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.823317805942321E-5 0.0 10-11 0.0 0.0 0.0 2.4558294514855805E-4 0.0 12-13 0.0 0.0 0.0 5.893990683565393E-4 0.0 14-15 0.0 0.0 0.0 5.893990683565393E-4 4.9116589029711605E-5 16-17 0.0 0.0 0.0 5.893990683565393E-4 9.823317805942321E-5 18-19 0.0 0.0 0.0 5.893990683565393E-4 9.823317805942321E-5 20-21 0.0 0.0 0.0 6.876322464159625E-4 1.4734976708913482E-4 22-23 0.0 0.0 0.0 0.0011787981367130786 1.9646635611884642E-4 24-25 0.0 0.0 0.0 0.0014243810818616366 1.9646635611884642E-4 26-27 0.0 0.0 0.0 0.0018173137940993294 1.9646635611884642E-4 28-29 0.0 0.0 0.0 0.003094345108871831 1.9646635611884642E-4 30-31 0.0 0.0 0.0 0.0067289726970704905 1.9646635611884642E-4 32-33 0.0 0.0 0.0 0.010510950052358282 2.9469953417826964E-4 34-35 0.0 0.0 0.0 0.013261479038022134 2.9469953417826964E-4 36-37 0.0 0.0 0.0 0.01748550569457733 2.9469953417826964E-4 38-39 0.0 0.0 0.0 0.0232812632000833 2.9469953417826964E-4 40-41 0.0 0.0 0.0 0.03202401604737197 2.9469953417826964E-4 42-43 0.0 0.0 0.0 0.04813425724911738 2.9469953417826964E-4 44-45 0.0 0.0 0.0 0.0685176416964477 2.9469953417826964E-4 46-47 0.0 0.0 0.0 0.08953954180116426 2.9469953417826964E-4 48-49 0.0 0.0 0.0 0.11650454917847594 2.9469953417826964E-4 50-51 0.0 0.0 0.0 0.15044411219800666 2.9469953417826964E-4 52-53 0.0 0.0 0.0 0.2149833101830477 2.9469953417826964E-4 54-55 0.0 0.0 0.0 0.261251137049036 2.9469953417826964E-4 56-57 0.0 0.0 0.0 0.35138007791855685 2.9469953417826964E-4 58-59 0.0 0.0 0.0 0.4622362193586159 2.9469953417826964E-4 60-61 0.0 0.0 0.0 0.5787898851261215 2.9469953417826964E-4 62-63 0.0 0.0 0.0 0.761110663604411 2.9469953417826964E-4 64-65 0.0 0.0 0.0 0.8710827064419353 2.9469953417826964E-4 66-67 0.0 0.0 0.0 1.1092981632360366 2.9469953417826964E-4 68-69 0.0 0.0 0.0 1.3657849911491908 2.9469953417826964E-4 70-71 0.0 0.0 0.0 1.5783615884697824 2.9469953417826964E-4 72-73 0.0 0.0 0.0 1.9472271720829166 2.9469953417826964E-4 74-75 0.0 0.0 0.0 2.300031631083335 2.9469953417826964E-4 76-77 0.0 0.0 0.0 2.5512138673812803 2.9469953417826964E-4 78-79 0.0 0.0 0.0 2.841591141724935 2.9469953417826964E-4 80-81 0.0 0.0 0.0 3.346067627649103 2.9469953417826964E-4 82-83 0.0 0.0 0.0 3.7592363745670374 2.9469953417826964E-4 84-85 0.0 0.0 0.0 4.357427312359895 2.9469953417826964E-4 86-87 0.0 0.0 0.0 5.073056014522793 2.9469953417826964E-4 88-89 0.0 0.0 0.0 6.079798739864792 2.9469953417826964E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3240 0.0 44.5979 1 GTATCAA 5415 0.0 34.848003 1 GACGCGT 65 0.0058399835 29.234638 8 TTGCGTA 215 0.0 28.719088 60-61 ATCAACG 6760 0.0 27.548025 3 TCAACGC 6810 0.0 27.345762 4 TATCAAC 6850 0.0 27.324785 2 CAACGCA 6900 0.0 26.98908 5 AACGCAG 7075 0.0 26.383467 6 GCGTAAG 235 0.0 26.274912 62-63 TGCGTAA 240 0.0 24.737997 60-61 CGTAAGT 255 0.0 24.214132 62-63 GTATAGG 300 0.0 23.765985 1 GTACATG 4870 0.0 23.033976 1 ACGCAGA 8180 0.0 22.649698 7 TATAGGG 210 3.4404366E-8 22.622042 2 GAGTACT 4550 0.0 22.50023 12-13 CGCAGAG 8190 0.0 22.390018 8 GTACTTT 4750 0.0 21.498623 14-15 TAATTCG 155 1.0913936E-11 21.450237 58-59 >>END_MODULE