FastQCFastQC Report
Thu 26 May 2016
SRR937449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202339
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84660.7041275380737047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61950.5152457002559179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42900.3568045285065194No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17880.14871013915376613No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.12550536911802743No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.050.317621
GTATCAA58800.043.1079941
ATCAACG74850.033.467813
TCAACGC75550.033.160494
TATCAAC76600.032.7045752
CAACGCA76400.032.6048855
AACGCAG77550.032.223816
ACGCAGA88850.028.0186237
CGCAGAG88850.027.9093538
GTACATG50200.025.6728171
GCAGAGT93000.025.5380389
TACATGG52400.023.7682192
GAGTACT61300.022.7033312-13
ACATGGG52050.022.5571773
GGAACGG2806.548362E-1122.05766
AGAGTAC86300.022.01565210-11
AGTACTT64700.021.43684812-13
GTACTTT65000.021.26483314-15
CAGAGTA91650.020.9378110-11
TGGTATC16750.020.7173732
Produced by FastQC (version 0.11.5)