##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937449_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1202339 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.682110453041947 33.0 31.0 34.0 30.0 34.0 2 31.77193952787026 33.0 31.0 34.0 30.0 34.0 3 31.80796181443004 33.0 31.0 34.0 30.0 34.0 4 35.43747645214869 37.0 35.0 37.0 33.0 37.0 5 35.42663092522159 37.0 35.0 37.0 33.0 37.0 6 35.4929291988366 37.0 35.0 37.0 33.0 37.0 7 35.439705440811615 37.0 35.0 37.0 33.0 37.0 8 35.433701310528896 37.0 35.0 37.0 33.0 37.0 9 37.0489088351954 39.0 37.0 39.0 33.0 39.0 10-11 36.95633594185999 39.0 37.0 39.0 33.0 39.0 12-13 36.850505140397175 39.0 37.0 39.0 33.0 39.0 14-15 38.00439767819226 40.0 38.0 41.0 33.0 41.0 16-17 37.958341615800535 40.0 38.0 41.0 33.0 41.0 18-19 37.93883214301457 40.0 38.0 41.0 33.0 41.0 20-21 37.886817694510455 40.0 38.0 41.0 33.0 41.0 22-23 37.75361815594437 40.0 38.0 41.0 32.5 41.0 24-25 37.617921817390936 40.0 37.0 41.0 32.0 41.0 26-27 37.4489278814045 40.0 37.0 41.0 32.0 41.0 28-29 37.261811352705024 39.5 37.0 41.0 32.0 41.0 30-31 37.21036122092022 39.0 37.0 41.0 32.0 41.0 32-33 37.03967849333674 39.0 36.0 41.0 31.0 41.0 34-35 36.83165604708822 39.0 36.0 41.0 31.0 41.0 36-37 36.82688368255542 39.0 36.0 41.0 31.0 41.0 38-39 36.80574779658649 39.5 36.0 41.0 31.0 41.0 40-41 36.63131529460493 39.5 35.5 41.0 30.5 41.0 42-43 36.38103272038917 39.0 35.0 41.0 30.0 41.0 44-45 36.10908529125313 39.0 35.0 41.0 29.5 41.0 46-47 35.72198148775013 39.0 35.0 41.0 27.5 41.0 48-49 35.36794157055539 39.0 35.0 41.0 26.0 41.0 50-51 34.43191936716683 38.0 34.0 40.0 24.5 40.5 52-53 34.56350538408884 38.5 34.5 40.0 24.0 40.5 54-55 34.928266903094716 39.0 35.0 40.0 24.5 41.0 56-57 34.7272071354252 39.0 34.5 40.0 23.5 41.0 58-59 34.50136026528292 38.0 34.0 40.0 23.0 41.0 60-61 34.24701519288653 38.0 34.0 40.0 22.5 41.0 62-63 33.941587605492295 38.0 34.0 40.0 22.0 41.0 64-65 33.59504848466197 37.5 33.5 40.0 20.0 41.0 66-67 33.22533827813953 37.0 33.0 40.0 18.5 41.0 68-69 32.751077275211074 36.0 32.5 39.0 16.5 41.0 70-71 32.344527209048366 36.0 32.0 39.0 13.0 40.5 72-73 31.943708887426922 35.0 32.0 38.5 9.0 40.0 74-75 31.482473744925514 35.0 31.0 37.5 7.5 39.5 76-77 31.052295983079645 35.0 31.0 37.0 4.5 39.0 78-79 30.58031803010632 35.0 31.0 36.5 2.0 39.0 80-81 30.127018669443476 34.5 30.0 36.0 2.0 38.5 82-83 29.67807997578054 34.0 30.0 35.5 2.0 37.0 84-85 29.22358211785528 34.0 29.0 35.0 2.0 37.0 86-87 28.808282855334475 34.0 29.0 35.0 2.0 36.0 88-89 28.4347184945344 34.0 29.0 35.0 2.0 36.0 90-91 28.02760411165237 34.0 28.0 35.0 2.0 36.0 92-93 27.61021725154054 34.0 27.0 35.0 2.0 35.0 94-95 27.111537178782356 33.5 26.0 35.0 2.0 35.0 96-97 26.542037644957038 33.0 25.0 35.0 2.0 35.0 98-99 25.87084424609033 33.0 24.5 35.0 2.0 35.0 100-101 24.081776853283476 30.5 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04722656909889977 1101 2 0.07822210918620698 1101 3 0.1943445710363818 1101 4 0.13205842590546268 1101 5 0.2264989623576028 1101 6 0.04477225085072689 1101 7 0.12545878064647553 1101 8 0.06620875194755627 1101 9 0.2361387113524529 1101 10-11 0.1760871368564878 1101 12-13 0.23904590244722357 1101 14-15 0.1470685801443068 1101 16-17 0.1442747348081994 1101 18-19 0.24235381195282457 1101 20-21 0.1914567330710497 1101 22-23 0.1424078836958742 1101 24-25 0.29034119221983445 1101 26-27 0.36563023726574073 1101 28-29 0.36730959052892587 1101 30-31 0.29175414739270167 1101 32-33 0.14025284829443052 1101 34-35 0.03113828340690361 1101 36-37 0.0640743558244452 1101 38-39 0.26894753619661316 1101 40-41 0.3955632308605246 1101 42-43 0.43764982324290713 1101 44-45 0.5070673356288395 1101 46-47 0.5673269185786012 1101 48-49 0.5704225387486446 1101 50-51 0.44613575902944547 1101 52-53 0.41993540672989127 1101 54-55 0.4444488056577285 1101 56-57 0.5327016869574877 1101 58-59 0.5014684576103363 1101 60-61 0.4257137033372089 1101 62-63 0.6229689236848657 1101 64-65 0.4642820775001937 1101 66-67 0.4936148740093458 1101 68-69 0.4479168883737401 1101 70-71 0.5453696972258513 1101 72-73 0.5964325858273725 1101 74-75 0.5685455456973081 1101 76-77 0.7225713276318615 1101 78-79 0.6809333858863909 1101 80-81 0.8153358921132927 1101 82-83 0.7651038520537021 1101 84-85 0.8036786088813201 1101 86-87 0.7999982713411526 1101 88-89 0.8985223939872533 1101 90-91 0.8728877335510035 1101 92-93 0.8460536351800414 1101 94-95 0.969092379905856 1101 96-97 1.2360705034867685 1101 98-99 1.2194183799790679 1101 100-101 1.4294471206678168 1102 1 0.24860225093316046 1102 2 0.0515469526453991 1102 3 0.10665229675180399 1102 4 -0.06113384382596365 1102 5 0.0465822780036973 1102 6 0.01572700444474151 1102 7 0.10521512406047862 1102 8 0.16838669886902835 1102 9 0.1915553140998938 1102 10-11 0.05389984546958004 1102 12-13 0.24321956520449106 1102 14-15 0.23326094163922306 1102 16-17 0.30646512934217895 1102 18-19 0.2996319176741764 1102 20-21 0.3771928689034283 1102 22-23 0.42246848262427505 1102 24-25 0.42469695297070587 1102 26-27 0.5184192519850299 1102 28-29 0.5485482645674935 1102 30-31 0.500023999303238 1102 32-33 0.4036571477292128 1102 34-35 0.3476843266141998 1102 36-37 0.44028347991741157 1102 38-39 0.6664267020314583 1102 40-41 0.6835356289627796 1102 42-43 0.5465566258596368 1102 44-45 0.4348084897333635 1102 46-47 0.42741736515858975 1102 48-49 0.5160680808992595 1102 50-51 0.4932570122245252 1102 52-53 0.47281534435873596 1102 54-55 0.6077888706486974 1102 56-57 0.5374147229070658 1102 58-59 0.47301424514073176 1102 60-61 0.5431173849445088 1102 62-63 0.6898620570438325 1102 64-65 0.6763005730973006 1102 66-67 0.784802146483166 1102 68-69 0.661904621486272 1102 70-71 0.6259865510269975 1102 72-73 0.42823448456110214 1102 74-75 0.46105494795272506 1102 76-77 0.49769088285211893 1102 78-79 0.6322257100454785 1102 80-81 0.8105878319304551 1102 82-83 0.7038767640315022 1102 84-85 0.6474684270565128 1102 86-87 0.6968571580048355 1102 88-89 0.8904508843097112 1102 90-91 0.8131127502400481 1102 92-93 0.6406293271012835 1102 94-95 0.48457046419237315 1102 96-97 0.5713499583415853 1102 98-99 0.6994860888268164 1102 100-101 0.8980855465947144 1103 1 0.19515708104364293 1103 2 0.11545429997094558 1103 3 0.12531254895677435 1103 4 0.1684420013820045 1103 5 0.112779760714254 1103 6 0.03251364475141827 1103 7 0.09567004208717123 1103 8 0.06007571648351018 1103 9 0.2425065496126848 1103 10-11 0.1185602821349434 1103 12-13 0.17810541545537006 1103 14-15 0.25513809398714926 1103 16-17 0.18729590031227872 1103 18-19 0.24301224378231723 1103 20-21 0.2505886956356136 1103 22-23 0.24408597720574932 1103 24-25 0.16966222532499842 1103 26-27 0.07480016866396255 1103 28-29 -0.04546592103239533 1103 30-31 -0.014501141608143087 1103 32-33 -0.017182935515982933 1103 34-35 0.22221889880739099 1103 36-37 0.21715593893819118 1103 38-39 0.293875822943761 1103 40-41 0.5281489389707943 1103 42-43 0.3745989952237352 1103 44-45 0.2580240212991427 1103 46-47 0.41691316347791485 1103 48-49 0.480545321609668 1103 50-51 0.5157463053395404 1103 52-53 0.45232462018802977 1103 54-55 0.5963210865090147 1103 56-57 0.7544149058798766 1103 58-59 0.6315858522222229 1103 60-61 0.6081805849456998 1103 62-63 0.5790582559694997 1103 64-65 0.6226501166321867 1103 66-67 0.7319936624947729 1103 68-69 0.6373420083369297 1103 70-71 0.6577226655471549 1103 72-73 0.9076005804418372 1103 74-75 0.9090174043107311 1103 76-77 0.9279596446319225 1103 78-79 0.9932024427459929 1103 80-81 0.9697860837218784 1103 82-83 1.0027252997283078 1103 84-85 0.9784387333946647 1103 86-87 1.0775458647142848 1103 88-89 1.095669031047958 1103 90-91 1.221502872088049 1103 92-93 1.2364453486771971 1103 94-95 1.3115510705613147 1103 96-97 1.3637687846081512 1103 98-99 1.2694955052327828 1103 100-101 1.5373892234557935 1104 1 0.1515501578381837 1104 2 0.09892902404915915 1104 3 0.09478770271295289 1104 4 -0.04796014639178736 1104 5 0.08440638442338866 1104 6 0.03078646253793238 1104 7 -0.042024034902190976 1104 8 0.10116578945945776 1104 9 0.0021752663617320422 1104 10-11 0.0803303012394494 1104 12-13 0.17564554760829054 1104 14-15 0.13069761863087592 1104 16-17 0.11378708690878625 1104 18-19 0.0769307374145356 1104 20-21 0.14629091494540347 1104 22-23 -0.0014757497300550426 1104 24-25 0.020497171234090672 1104 26-27 0.1751496092740794 1104 28-29 -0.007293500043473955 1104 30-31 0.18937662502127495 1104 32-33 0.021238398379985313 1104 34-35 0.2035818095906876 1104 36-37 -0.03356962389759133 1104 38-39 0.07551952596698897 1104 40-41 0.07896394104602678 1104 42-43 0.009254332663395815 1104 44-45 0.12961730261424265 1104 46-47 0.2891877307775772 1104 48-49 0.4530586373914005 1104 50-51 0.41484513666168255 1104 52-53 0.3655296712912133 1104 54-55 0.5190830071849248 1104 56-57 0.6139156016039706 1104 58-59 0.6636515328883306 1104 60-61 0.6758856656951338 1104 62-63 0.6433511315546241 1104 64-65 0.5974900846912519 1104 66-67 0.7212732742753403 1104 68-69 0.7687553177273898 1104 70-71 0.737559796037587 1104 72-73 0.6735123332122157 1104 74-75 0.5790139468661799 1104 76-77 0.6700211902251425 1104 78-79 0.5194093334560925 1104 80-81 0.6123550420300212 1104 82-83 0.4980496642399892 1104 84-85 0.5811067409440618 1104 86-87 0.5699142681372322 1104 88-89 0.5674240576256189 1104 90-91 0.9115389249954191 1104 92-93 0.723305326792886 1104 94-95 0.6227108776528496 1104 96-97 0.6617760567922026 1104 98-99 0.47846222213122047 1104 100-101 0.693875551149155 1105 1 -0.046177401467311086 1105 2 -0.020190727270719577 1105 3 -0.14592928110858594 1105 4 -0.15886840996201812 1105 5 -0.04109922677817934 1105 6 -0.15030101957584918 1105 7 -0.09247495950320683 1105 8 -0.05985020260207108 1105 9 -0.060660871201797306 1105 10-11 0.014195972308449711 1105 12-13 0.04531024610557921 1105 14-15 0.015795177333309596 1105 16-17 0.06303901807220313 1105 18-19 0.086906724651584 1105 20-21 -0.07623228916856561 1105 22-23 -0.006948795677764963 1105 24-25 0.08305014459526916 1105 26-27 0.04614460387394814 1105 28-29 0.03370061737783914 1105 30-31 -0.0429032716909461 1105 32-33 -0.06253133128453925 1105 34-35 0.13245222290625236 1105 36-37 0.03115025461318055 1105 38-39 0.03111071690134537 1105 40-41 0.061381719262392664 1105 42-43 0.18452091270867754 1105 44-45 0.17560695190729092 1105 46-47 0.06587890362013127 1105 48-49 -0.0976675019813058 1105 50-51 -0.2725454216076457 1105 52-53 -0.17555627576009414 1105 54-55 0.06516503434455956 1105 56-57 -0.08993411578006061 1105 58-59 -0.02800567669702758 1105 60-61 0.23490717301682906 1105 62-63 0.36188728501701917 1105 64-65 0.19588402558037643 1105 66-67 0.3886260590431334 1105 68-69 0.33286226772273153 1105 70-71 0.3205406132390536 1105 72-73 0.19087388976556596 1105 74-75 0.08338202195266575 1105 76-77 0.013609289105097844 1105 78-79 0.19116829575386163 1105 80-81 -0.11800493011955737 1105 82-83 -0.12895455169944015 1105 84-85 0.04056409636036307 1105 86-87 -0.10325722575267093 1105 88-89 0.02899979925267715 1105 90-91 0.07080823525895497 1105 92-93 -0.03316249446005415 1105 94-95 0.12676536140585526 1105 96-97 -0.04376140695565667 1105 98-99 0.05102709222655477 1105 100-101 0.11949437320800627 1106 1 -0.1284941185666355 1106 2 -0.024984484238391502 1106 3 -0.2777443259671095 1106 4 -0.14042067412297854 1106 5 -0.10289781466372716 1106 6 -0.03918459976377875 1106 7 0.03817747984488307 1106 8 -0.07135946628525147 1106 9 0.0813203359968 1106 10-11 -0.1466762472594212 1106 12-13 -0.287693947768183 1106 14-15 -0.29205904573830566 1106 16-17 -0.36065371431617876 1106 18-19 -0.22336561170770608 1106 20-21 -0.14208141588725454 1106 22-23 -0.14191190020608957 1106 24-25 -0.17481469587826126 1106 26-27 0.11574698217197721 1106 28-29 0.18628330039530283 1106 30-31 0.11793444132067776 1106 32-33 0.1753462500712928 1106 34-35 0.1327309297067103 1106 36-37 -0.26931691107137823 1106 38-39 -0.2332785170531082 1106 40-41 -0.057894173926541725 1106 42-43 -0.33316237019199235 1106 44-45 -0.18450081472222024 1106 46-47 -0.21802776941697743 1106 48-49 -0.5182281008027729 1106 50-51 -0.7368895316039286 1106 52-53 -0.7528748509047531 1106 54-55 -0.5660395099034616 1106 56-57 -0.5857428963714497 1106 58-59 -0.5503738881956792 1106 60-61 -0.5859666391613274 1106 62-63 -0.42627431184023123 1106 64-65 -0.3282519833382409 1106 66-67 -0.3703907695389077 1106 68-69 -0.17523084689211998 1106 70-71 -0.5173343137663089 1106 72-73 -0.40395012679758224 1106 74-75 -0.3780104503093753 1106 76-77 -0.31339341833239587 1106 78-79 -0.3663196269514515 1106 80-81 -0.1834665216681053 1106 82-83 -0.09643610396360103 1106 84-85 -0.2435761595190229 1106 86-87 -0.39697437816547776 1106 88-89 -0.0379800807706836 1106 90-91 -0.03879255042878427 1106 92-93 -0.07198237023645149 1106 94-95 0.0491548077083479 1106 96-97 0.23140714429985465 1106 98-99 0.19001153773274027 1106 100-101 0.27725038531184154 1107 1 -6.60780984890863E-4 1107 2 -0.15332763140625616 1107 3 -0.12092548113898616 1107 4 -0.020619669798094264 1107 5 0.15566202990099498 1107 6 0.10202982331586696 1107 7 9.996157771112735E-4 1107 8 0.11092861578276114 1107 9 -0.005783464067206978 1107 10-11 0.03640416387369072 1107 12-13 -0.013817865484917036 1107 14-15 0.12753123729098093 1107 16-17 0.14014215509325822 1107 18-19 0.27079777660009086 1107 20-21 0.14461142100483215 1107 22-23 0.07981561789923575 1107 24-25 0.11499151983350231 1107 26-27 0.014664486484115002 1107 28-29 0.14485206662778438 1107 30-31 0.065295893484965 1107 32-33 0.09331009890708941 1107 34-35 0.33786981033479435 1107 36-37 0.39341714109346526 1107 38-39 0.2635776571658184 1107 40-41 0.2501051633209954 1107 42-43 0.3596837267907844 1107 44-45 0.4382216817586766 1107 46-47 0.5313845334212459 1107 48-49 0.39688860310831586 1107 50-51 0.2823366624126109 1107 52-53 0.6191494896707042 1107 54-55 0.563908121322946 1107 56-57 0.3897062317125446 1107 58-59 0.34598323908394946 1107 60-61 0.5665825503830391 1107 62-63 0.6046661735551382 1107 64-65 0.4983306213922347 1107 66-67 0.7325659844468149 1107 68-69 0.6426743738412668 1107 70-71 0.6333710644415973 1107 72-73 0.5092505427523406 1107 74-75 0.5452858651834589 1107 76-77 0.5039807507487879 1107 78-79 0.3776632037945973 1107 80-81 0.5075244348762915 1107 82-83 0.6694664147789808 1107 84-85 0.8336691976733981 1107 86-87 0.8615082868924588 1107 88-89 0.7854077172200995 1107 90-91 0.8876228084346778 1107 92-93 0.9809733924528494 1107 94-95 0.8134146066734367 1107 96-97 0.9670170606627586 1107 98-99 1.053576579522968 1107 100-101 1.0019192215015238 1108 1 -0.0574989187693582 1108 2 -0.1353934661174101 1108 3 -0.1902810041807541 1108 4 -0.15965891909745267 1108 5 -0.028871892993130643 1108 6 0.03779141649989981 1108 7 -0.024094136184970694 1108 8 -0.058978859449787535 1108 9 0.0633361331846416 1108 10-11 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-0.9279779281817468 2316 54-55 -1.0359745667950406 2316 56-57 -1.073267449113395 2316 58-59 -1.0666096653010229 2316 60-61 -1.1608193227096706 2316 62-63 -1.142528669398942 2316 64-65 -1.0261957464993898 2316 66-67 -1.3643653939483187 2316 68-69 -1.0541747693143044 2316 70-71 -1.2447015520031144 2316 72-73 -1.646305597413921 2316 74-75 -1.5782988897282486 2316 76-77 -1.3225730470772383 2316 78-79 -1.2645297099441422 2316 80-81 -1.350341112455741 2316 82-83 -1.3080143807592712 2316 84-85 -1.6746780688818 2316 86-87 -1.671633294128739 2316 88-89 -1.466299346046469 2316 90-91 -1.1625250980743793 2316 92-93 -1.420626881924445 2316 94-95 -0.9183913337508365 2316 96-97 -1.3883483015425497 2316 98-99 -1.0974059562064937 2316 100-101 -1.4554344017207725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5185.0 3 1016.0 4 866.0 5 996.0 6 1103.0 7 1284.0 8 1581.0 9 1951.0 10 2432.0 11 3232.0 12 4399.0 13 5893.0 14 6857.0 15 8509.0 16 11124.0 17 15334.0 18 12931.0 19 7999.0 20 7558.0 21 7783.0 22 8148.0 23 9109.0 24 9991.0 25 11347.0 26 13060.0 27 15164.0 28 17856.0 29 21275.0 30 25999.0 31 32545.0 32 41220.0 33 54061.0 34 74115.0 35 105971.0 36 159423.0 37 238939.0 38 221233.0 39 34840.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63655370131577 19.55907207331272 15.461522090213704 22.342852135157806 2 14.711744217573571 20.28314597367927 41.594760881807 23.410348926940163 3 18.222318627778147 25.376259761062535 31.06996262430755 25.331458986851764 4 11.77427576707411 16.85539869832882 38.93049716630806 32.439828368289014 5 12.644706000116782 37.35996529892143 35.39351523594231 14.601813465019479 6 29.87344557609644 37.32534480933484 18.364853161693425 14.436356452875293 7 26.894542483932543 33.3719563532089 21.968857064261478 17.764644098597067 8 25.60770676033656 34.42418523698938 20.897510490349877 19.07059751232418 9 25.968800663496445 16.442208709641122 21.489782904964137 36.0992077218983 10-11 23.744132365335126 26.966986192771024 28.550528556030443 20.73835288586341 12-13 24.93642611612232 24.639324234935565 29.034873208604452 21.389376440337664 14-15 22.007286935482327 25.555240230084802 27.89080181710817 24.5466710173247 16-17 20.654621659256804 29.026428356185335 28.479514054013727 21.839435930544134 18-19 20.454387286024037 26.86004949935969 31.985137690149344 20.70042552446693 20-21 21.086504708667725 26.03342931122216 32.21418864093559 20.665877339174525 22-23 20.815111166677084 25.946364943426893 32.12564855910483 21.11287533079119 24-25 20.662102359382946 26.178399686637942 32.17761628149247 20.981881672486644 26-27 20.443947289125873 26.49067757370608 31.972523462873877 21.092851674294174 28-29 20.250850198379624 26.33872903677525 32.39039109125463 21.020029673590503 30-31 20.408551999516504 26.282080939311147 32.24918254171694 21.0601845194554 32-33 20.197308998362356 26.42876881735892 32.01242423977182 21.361497944506898 34-35 20.704130907436017 26.419078665236924 31.839714740214305 21.037075687112758 36-37 20.630105017502917 26.211951991998667 31.800675112518757 21.357267877979663 38-39 20.348685065855047 26.63637545545158 31.874068678598693 21.140870800094675 40-41 20.604915564008092 26.398883909782704 32.01654633749562 20.979654188713585 42-43 20.40359323655474 26.416947449040396 31.625832033942235 21.55362728046263 44-45 20.57781232352322 26.71940218263354 31.27567574326028 21.42710975058296 46-47 20.616607985158 26.876742161549245 30.84652642834848 21.660123424944274 48-49 20.76360120362421 27.09467287927916 30.551966725291948 21.589759191804685 50-51 20.766977168158043 27.181433097272173 30.350648977153988 21.700940757415797 52-53 21.023102915564433 27.337616375382567 30.114660025895006 21.52462068315799 54-55 20.733226181861834 27.261935328973912 30.24543411351609 21.75940437564816 56-57 20.7148678581131 27.636296060493432 30.063133438555735 21.585702642837738 58-59 20.87651726141995 27.130498542887437 30.293234789890413 21.699749405802205 60-61 20.900984307085142 27.133276445961442 30.522727727260605 21.44301151969281 62-63 20.770088439381983 27.073383937581614 30.710956633620295 21.445570989416108 64-65 20.636780317123566 26.93054604799643 30.528673988193173 21.903999646686835 66-67 20.54356304941346 27.385833675023918 30.184112887902153 21.88649038766047 68-69 20.564235894102648 27.124030232577518 30.488112797180072 21.823621076139762 70-71 20.573787671144405 27.18351696933307 30.348878537734375 21.89381682178815 72-73 20.726284687928455 27.06110576301982 30.186321943982563 22.026287605069157 74-75 20.51752163979497 27.188491333456394 30.097826499118074 22.19616052763056 76-77 20.600245789223866 27.103075199083854 30.260037330957402 22.036641680734885 78-79 20.536216960464866 27.00775067165681 30.07747337855357 22.378558989324752 80-81 20.480201800756753 27.256102210383286 30.053696034693463 22.209999954166495 82-83 20.710015773034986 27.189420189901508 30.08557444476625 22.01498959229726 84-85 20.49371574658997 26.990707987830636 29.858807412610837 22.656768852968554 86-87 20.49303755802035 26.92294230881409 30.215331538903843 22.368688594261716 88-89 20.62029439168996 26.639931060806838 30.124602257858584 22.61517228964462 90-91 20.444744648517467 26.564087990536073 30.59371652133113 22.397450839615328 92-93 20.5543512413604 26.35677037103712 30.46889910680725 22.61997928079523 94-95 20.189775808522366 26.378304002160196 30.413592251619836 23.018327937697595 96-97 20.17844449203779 26.35404227233598 30.49802455205541 22.969488683570823 98-99 20.035669936410837 26.690988340597887 30.32465476001967 22.948686962971607 100-101 19.896457808806918 26.877597981152256 30.36904300134032 22.85690120870051 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1856.0 1 1086.5 2 361.0 3 424.0 4 353.5 5 297.5 6 558.5 7 1266.5 8 2248.0 9 2773.5 10 2459.0 11 2047.5 12 2052.0 13 2244.0 14 2409.0 15 2517.5 16 2603.0 17 2687.5 18 2801.0 19 2931.5 20 3293.0 21 3744.5 22 4277.0 23 5032.5 24 5810.0 25 6953.0 26 8701.5 27 10765.0 28 12324.0 29 14181.5 30 16829.0 31 19300.0 32 22087.0 33 25691.0 34 29789.0 35 32683.5 36 35916.5 37 39223.0 38 40585.0 39 40967.0 40 40694.5 41 41527.5 42 42495.0 43 43400.5 44 44294.5 45 44636.0 46 43863.0 47 42865.5 48 44698.5 49 44759.0 50 42008.5 51 39292.5 52 36128.0 53 34163.5 54 32471.5 55 29197.5 56 25519.5 57 21935.0 58 18902.5 59 16516.5 60 14162.5 61 11543.5 62 8782.0 63 6543.5 64 4950.0 65 3726.5 66 2692.0 67 2024.0 68 1506.0 69 987.5 70 685.0 71 534.0 72 449.5 73 359.0 74 250.0 75 199.5 76 137.5 77 75.5 78 44.5 79 30.5 80 20.0 81 14.0 82 12.5 83 8.5 84 7.0 85 4.0 86 5.0 87 5.0 88 2.5 89 1.5 90 1.0 91 0.5 92 0.0 93 1.5 94 1.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.347988379317314 2 0.304739345558948 3 0.3076503382157611 4 0.3098959611224455 5 0.29351123102552606 6 0.2177422507296195 7 0.22531083163733356 8 0.22165129801162567 9 0.21907299022987692 10-11 0.22872085160674321 12-13 0.2287624372161262 14-15 0.20963305689992592 16-17 0.22452070505905572 18-19 0.21004891299375633 20-21 0.21487284368218945 22-23 0.21325100491625076 24-25 0.20368631475815058 26-27 0.2182828636515991 28-29 0.2178254219483856 30-31 0.227598040153401 32-33 0.22859609477859405 34-35 0.21545504221355208 36-37 0.21117172444709856 38-39 0.20218923282036097 40-41 0.20260508891419143 42-43 0.20110800697640185 44-45 0.21969677437062263 46-47 0.20796963252460413 48-49 0.2186571341360465 50-51 0.22626730065314357 52-53 0.2395331100463347 54-55 0.2321308715761528 56-57 0.19470382313141302 58-59 0.1822697259258828 60-61 0.19187600169336602 62-63 0.1919591729121321 64-65 0.18884025220840378 66-67 0.2015238630702323 68-69 0.19204234413089818 70-71 0.19674151799118217 72-73 0.2107558683532681 74-75 0.200317880398124 76-77 0.21046476908758677 78-79 0.20855183105596675 80-81 0.19491175117832824 82-83 0.20846865983720064 84-85 0.18929769391161727 86-87 0.19370576850621996 88-89 0.20302094500802187 90-91 0.20081690771072053 92-93 0.20676364985249585 94-95 0.2037279003675336 96-97 0.19611773385043654 98-99 0.20368631475815058 100-101 0.18701048539554985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1202339.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.293565815831805 #Duplication Level Percentage of deduplicated Percentage of total 1 80.60606691833111 46.18209000140745 2 11.164289746384986 12.792839387430485 3 3.4008459740057084 5.845397779236079 4 1.444843749534077 3.3112100183009536 5 0.8006361107107279 2.2935648851768344 6 0.497863274668958 1.7114617376718895 7 0.3380856332492419 1.3559092035966829 8 0.24239723840064004 1.1110241705506352 9 0.18861495365001224 0.9725780934717334 >10 1.146325204849133 12.590360224268368 >50 0.11606019398011766 4.513662368080916 >100 0.05251626048272644 5.173874760029928 >500 5.818967010430919E-4 0.1951161284368306 >1k 5.818967010430919E-4 0.7289558561762156 >5k 2.9094835052154595E-4 1.2219553861650463 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8466 0.7041275380737047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6195 0.5152457002559179 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4290 0.3568045285065194 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1788 0.14871013915376613 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1509 0.12550536911802743 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.317121876608844E-5 0.0 10-11 0.0 0.0 0.0 8.317121876608844E-5 0.0 12-13 0.0 0.0 0.0 8.317121876608844E-5 0.0 14-15 0.0 0.0 0.0 8.317121876608844E-5 0.0 16-17 0.0 0.0 0.0 8.317121876608844E-5 0.0 18-19 0.0 0.0 0.0 8.317121876608844E-5 0.0 20-21 0.0 0.0 0.0 1.2475682814913267E-4 0.0 22-23 0.0 0.0 0.0 4.5744170321348636E-4 0.0 24-25 0.0 0.0 0.0 6.653697501287075E-4 0.0 26-27 0.0 0.0 0.0 8.317121876608843E-4 0.0 28-29 0.0 0.0 0.0 0.0020792804691522108 0.0 30-31 0.0 0.0 0.0 0.005406129219795748 0.0 32-33 0.0 0.0 0.0 0.00877456357982233 0.0 34-35 0.0 0.0 0.0 0.012600439643062396 0.0 36-37 0.0 0.0 0.0 0.017465955940878568 0.0 38-39 0.0 0.0 0.0 0.024202824660931734 0.0 40-41 0.0 0.0 0.0 0.030731765334069676 0.0 42-43 0.0 0.0 0.0 0.046783810555924746 0.0 44-45 0.0 0.0 0.0 0.06699441671608423 0.0 46-47 0.0 0.0 0.0 0.08620696825105066 4.158560938304422E-5 48-49 0.0 0.0 0.0 0.11402774092830725 8.317121876608844E-5 50-51 0.0 0.0 0.0 0.14871013915376613 8.317121876608844E-5 52-53 0.0 0.0 0.0 0.20576559522730278 8.317121876608844E-5 54-55 0.0 0.0 0.0 0.24701851973528266 8.317121876608844E-5 56-57 0.0 0.0 0.0 0.331977919704842 8.317121876608844E-5 58-59 0.0 0.0 0.0 0.4423045413980583 8.317121876608844E-5 60-61 0.0 0.0 0.0 0.5515083516379324 8.317121876608844E-5 62-63 0.0 0.0 0.0 0.7132347865285913 8.317121876608844E-5 64-65 0.0 0.0 0.0 0.8202345594711642 8.317121876608844E-5 66-67 0.0 0.0 0.0 1.0400976762793188 8.317121876608844E-5 68-69 0.0 0.0 0.0 1.2845378882328529 8.317121876608844E-5 70-71 0.0 0.0 0.0 1.4789090264892015 8.317121876608844E-5 72-73 0.0 0.0 0.0 1.7968310102225744 8.317121876608844E-5 74-75 0.0 0.0 0.0 2.1147114083465643 8.317121876608844E-5 76-77 0.0 0.0 0.0 2.354244518392899 8.317121876608844E-5 78-79 0.0 0.0 0.0 2.6270045303362863 8.317121876608844E-5 80-81 0.0 0.0 0.0 3.091432615926124 8.317121876608844E-5 82-83 0.0 0.0 0.0 3.46458028892018 8.317121876608844E-5 84-85 0.0 0.0 0.0 3.9938403395381834 8.317121876608844E-5 86-87 0.0 0.0 0.0 4.640247051788223 8.317121876608844E-5 88-89 0.0 0.0 0.0 5.525604675553234 8.317121876608844E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3100 0.0 50.31762 1 GTATCAA 5880 0.0 43.107994 1 ATCAACG 7485 0.0 33.46781 3 TCAACGC 7555 0.0 33.16049 4 TATCAAC 7660 0.0 32.704575 2 CAACGCA 7640 0.0 32.604885 5 AACGCAG 7755 0.0 32.22381 6 ACGCAGA 8885 0.0 28.018623 7 CGCAGAG 8885 0.0 27.909353 8 GTACATG 5020 0.0 25.672817 1 GCAGAGT 9300 0.0 25.538038 9 TACATGG 5240 0.0 23.768219 2 GAGTACT 6130 0.0 22.70333 12-13 ACATGGG 5205 0.0 22.557177 3 GGAACGG 280 6.548362E-11 22.0576 6 AGAGTAC 8630 0.0 22.015652 10-11 AGTACTT 6470 0.0 21.436848 12-13 GTACTTT 6500 0.0 21.264833 14-15 CAGAGTA 9165 0.0 20.93781 10-11 TGGTATC 1675 0.0 20.717373 2 >>END_MODULE