FastQCFastQC Report
Thu 26 May 2016
SRR937449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202339
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60950.506928578379309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.41785220308082827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32570.27088865952115No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12610.10487890686403752No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38250.038.397511
GTATCAA61350.035.9483871
GTGTAGC4200.029.4238911
ATCAACG78450.027.856343
TCAACGC78800.027.6723274
TATCAAC79650.027.4963072
CAACGCA79700.027.3598425
AACGCAG82150.026.6539926
GTACATG56850.025.8347361
TACATGG60900.023.5585672
ACATGGG59800.023.356373
ACGCAGA93600.023.1406737
CGCAGAG94100.022.9672348
GTATAGG5200.021.9373381
GCAGAGT98150.021.6323749
GAGTACT52750.021.38592712-13
AGTACTT55200.020.9530312-13
GACGAGT2309.026735E-820.6509976
CATGGGG24800.020.1139264
GTACTTT55500.020.02588714-15
Produced by FastQC (version 0.11.5)