##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937449_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1202339 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16593573027241 33.0 31.0 34.0 31.0 34.0 2 32.386794406569194 34.0 31.0 34.0 31.0 34.0 3 32.46360968079718 34.0 31.0 34.0 31.0 34.0 4 36.08024276015333 37.0 35.0 37.0 35.0 37.0 5 35.970727057843085 37.0 35.0 37.0 35.0 37.0 6 36.05325702651249 37.0 35.0 37.0 35.0 37.0 7 36.00583279757207 37.0 35.0 37.0 35.0 37.0 8 35.98514312519181 37.0 35.0 37.0 35.0 37.0 9 37.682849845176776 39.0 37.0 39.0 35.0 39.0 10-11 37.61027463968149 39.0 37.0 39.0 35.0 39.0 12-13 37.53556110215172 39.0 37.0 39.0 35.0 39.0 14-15 38.74729714331815 40.0 38.0 41.0 35.0 41.0 16-17 38.72339664603743 40.0 38.0 41.0 35.0 41.0 18-19 38.73031233287783 40.0 38.0 41.0 34.5 41.0 20-21 38.629392791883156 40.0 38.0 41.0 34.0 41.0 22-23 38.50242527273922 40.0 38.0 41.0 34.0 41.0 24-25 38.370291989197725 40.0 38.0 41.0 34.0 41.0 26-27 38.32463099009514 40.0 38.0 41.0 34.0 41.0 28-29 38.234859303407774 40.0 38.0 41.0 34.0 41.0 30-31 38.30618236620454 40.0 38.0 41.0 34.0 41.0 32-33 38.243473762391474 40.0 38.0 41.0 34.0 41.0 34-35 38.13819064340423 40.0 38.0 41.0 34.0 41.0 36-37 37.9852246329862 40.0 37.0 41.0 33.0 41.0 38-39 37.868145340041366 40.0 37.0 41.0 33.0 41.0 40-41 37.69692116782372 40.0 37.0 41.0 33.0 41.0 42-43 37.490056880796516 40.0 37.0 41.0 33.0 41.0 44-45 37.18986866432844 40.0 37.0 41.0 32.0 41.0 46-47 36.831213160348284 40.0 36.0 41.0 31.0 41.0 48-49 36.52486154071356 40.0 36.0 41.0 30.5 41.0 50-51 36.30634704521769 39.0 36.0 41.0 30.0 41.0 52-53 36.082565316437375 39.0 35.5 41.0 29.5 41.0 54-55 35.85394884471019 39.0 35.0 40.0 29.0 41.0 56-57 35.62036788293484 39.0 35.0 40.0 28.0 41.0 58-59 35.38607414381468 38.5 35.0 40.0 28.0 41.0 60-61 35.071071885716094 38.0 34.5 40.0 27.5 41.0 62-63 34.7149460343547 38.0 34.0 40.0 26.5 41.0 64-65 34.35545341205767 37.0 34.0 40.0 26.0 41.0 66-67 33.94959782557166 37.0 33.5 39.5 26.0 41.0 68-69 33.55162271206373 36.0 33.0 39.0 25.0 40.0 70-71 33.150689198304306 36.0 33.0 39.0 24.5 40.0 72-73 32.705124760986706 35.0 32.0 38.0 23.5 40.0 74-75 32.24545989109561 35.0 32.0 37.0 22.0 39.5 76-77 30.90680124324338 34.0 30.5 36.0 20.5 38.5 78-79 31.539126236444133 35.0 31.5 36.0 21.0 39.0 80-81 31.39055166637695 35.0 31.5 36.0 20.5 38.0 82-83 31.059343912157885 34.5 31.0 35.5 20.0 37.0 84-85 30.697372371685525 34.0 31.0 35.0 18.0 37.0 86-87 30.320825075124404 34.0 31.0 35.0 17.0 36.0 88-89 29.937167886927064 34.0 30.5 35.0 9.0 36.0 90-91 29.521495601490095 34.0 30.0 35.0 6.0 35.5 92-93 29.16823167176645 34.0 29.5 35.0 2.0 35.0 94-95 28.765017603188454 34.0 29.0 35.0 2.0 35.0 96-97 28.307490233619635 34.0 29.0 35.0 2.0 35.0 98-99 27.828563741174495 34.0 29.0 35.0 2.0 35.0 100-101 25.948448815184406 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20268717102588596 1101 2 0.2257979144347928 1101 3 0.1774313165617727 1101 4 0.1394683661885665 1101 5 0.06513135754824617 1101 6 0.13324212831910387 1101 7 0.17416462255179255 1101 8 0.11954752043270389 1101 9 0.20024601041159684 1101 10-11 0.23098995652382825 1101 12-13 0.11584116322767102 1101 14-15 0.166365688282049 1101 16-17 0.21156571032980054 1101 18-19 0.08690008191670273 1101 20-21 0.06811384744877813 1101 22-23 0.16606004301003452 1101 24-25 0.22254227369023738 1101 26-27 0.06154926341935152 1101 28-29 0.10089984173718136 1101 30-31 0.29063214475399235 1101 32-33 0.26672563929520976 1101 34-35 0.14989321815040313 1101 36-37 0.02999749040782973 1101 38-39 0.08787118437068386 1101 40-41 0.03903370024019637 1101 42-43 0.05964022035902161 1101 44-45 0.11784155465483792 1101 46-47 0.22667854986301705 1101 48-49 0.0768780604501913 1101 50-51 -0.05284408384925854 1101 52-53 -0.00927802287560553 1101 54-55 -0.057114312842728054 1101 56-57 0.06656434990681248 1101 58-59 0.05978703862248835 1101 60-61 0.20664733320001005 1101 62-63 0.197775270774315 1101 64-65 0.051625698532909325 1101 66-67 0.045894759607001845 1101 68-69 -0.002334266996292911 1101 70-71 -0.04109501389677206 1101 72-73 -0.05051544380233963 1101 74-75 -0.14558501086686704 1101 76-77 -0.05611147559485019 1101 78-79 -0.004595463284822188 1101 80-81 -0.04565657985525107 1101 82-83 0.01324043566968669 1101 84-85 0.07161978642613676 1101 86-87 0.10679512930245494 1101 88-89 0.16079549691981754 1101 90-91 0.15212263885787536 1101 92-93 -0.08709195314286333 1101 94-95 -0.2407975695223179 1101 96-97 -0.24223843765596698 1101 98-99 -0.2754204994397931 1101 100-101 0.04866720681940606 1102 1 0.12416984488793048 1102 2 0.14201504506748108 1102 3 0.15920225387329623 1102 4 0.131392828724195 1102 5 0.038575633642878415 1102 6 0.07926698291600331 1102 7 0.06672043191629484 1102 8 0.09661260176248021 1102 9 0.19117403351077655 1102 10-11 0.09289273205421722 1102 12-13 0.10524177951408831 1102 14-15 0.09203025559617828 1102 16-17 0.008550894378643648 1102 18-19 0.1522517987695906 1102 20-21 0.15659872013218745 1102 22-23 0.0920429806045604 1102 24-25 0.0673514721746713 1102 26-27 0.006178227199399089 1102 28-29 -0.1125782887813287 1102 30-31 -0.011087264905548011 1102 32-33 -0.08885946503847464 1102 34-35 -0.031631253631623224 1102 36-37 -0.02957120016917969 1102 38-39 -0.2104661102034342 1102 40-41 -0.14153671142763358 1102 42-43 -0.06603513441894648 1102 44-45 -0.18566321529548446 1102 46-47 -0.12446092854208501 1102 48-49 -0.07191521461682981 1102 50-51 -0.02673544428154173 1102 52-53 0.03066588931724823 1102 54-55 -0.07349041847021454 1102 56-57 -0.006423700525282072 1102 58-59 -0.12823285234007642 1102 60-61 -0.2747875465933234 1102 62-63 -0.3965632020150096 1102 64-65 -0.2715816035994294 1102 66-67 -0.20529851602041305 1102 68-69 -0.1343152096104916 1102 70-71 -0.28836119736912025 1102 72-73 -0.15516400013847687 1102 74-75 -0.19303058846200827 1102 76-77 -0.12242793495308035 1102 78-79 -0.017430268686677408 1102 80-81 0.034118291378156584 1102 82-83 0.18861360136302707 1102 84-85 0.047472328800207464 1102 86-87 -0.14573414570292442 1102 88-89 -0.1488675839658491 1102 90-91 0.04135149630434398 1102 92-93 0.0012015121057444844 1102 94-95 0.027894163287612628 1102 96-97 0.1012400150854873 1102 98-99 0.2526398655267279 1102 100-101 0.5137870982722994 1103 1 0.12909821028613067 1103 2 0.12995222737534817 1103 3 0.12171960282031336 1103 4 0.1538218746920208 1103 5 -0.0052426371598670585 1103 6 0.08674506708646845 1103 7 0.08593034636534469 1103 8 0.0102776960529809 1103 9 0.15079728801704562 1103 10-11 0.07260766635507565 1103 12-13 0.13088177528933898 1103 14-15 0.1295857982593347 1103 16-17 0.03615003812536344 1103 18-19 0.13345431695157828 1103 20-21 0.1483403982160567 1103 22-23 0.1355339829534543 1103 24-25 0.20582953998171405 1103 26-27 0.25500861437285494 1103 28-29 0.007879931681578967 1103 30-31 0.047533209306436675 1103 32-33 -0.012944340624393647 1103 34-35 0.06591966264566906 1103 36-37 0.12178264992103749 1103 38-39 0.15151139455311835 1103 40-41 0.102150354476791 1103 42-43 0.10962658008097748 1103 44-45 0.10044528014730503 1103 46-47 -0.07122885504354315 1103 48-49 -0.020425473739067 1103 50-51 0.04051785108865147 1103 52-53 0.03653329204041711 1103 54-55 -0.04522334956087093 1103 56-57 -0.15716131899274188 1103 58-59 0.04182513367960894 1103 60-61 -0.1826536670191885 1103 62-63 -0.065493971348765 1103 64-65 -0.18689084997456717 1103 66-67 -0.23236250146588588 1103 68-69 0.0685975540079724 1103 70-71 -0.11015757391229641 1103 72-73 0.11978240965220976 1103 74-75 0.13829971317537826 1103 76-77 0.11289258730786855 1103 78-79 0.07706552219472584 1103 80-81 0.009220599959583353 1103 82-83 0.043583098455545866 1103 84-85 0.2262616884102222 1103 86-87 0.24895532859980918 1103 88-89 0.15557724229179115 1103 90-91 0.23072575902058645 1103 92-93 0.2023325221305683 1103 94-95 0.13513222579886275 1103 96-97 0.042631813440518584 1103 98-99 0.18699018558039882 1103 100-101 0.38721602374583597 1104 1 0.05783558079559725 1104 2 0.06286317238195949 1104 3 0.019498983976234285 1104 4 0.01926259876869807 1104 5 -0.025604213477372184 1104 6 0.036201693868036955 1104 7 -0.05889268868575215 1104 8 -0.05729686423938318 1104 9 0.06662083359948667 1104 10-11 -0.03999298647821803 1104 12-13 -0.07013435919523658 1104 14-15 -0.14124569020519573 1104 16-17 -0.11325930867943157 1104 18-19 -0.17369785758083367 1104 20-21 -0.17502769180378408 1104 22-23 -0.08130631313932213 1104 24-25 -0.12568703072610532 1104 26-27 -0.24825420454560287 1104 28-29 -0.2212412228738927 1104 30-31 -0.22012651654670634 1104 32-33 -0.09120444517936477 1104 34-35 -0.1575760217088984 1104 36-37 -0.19533393372332597 1104 38-39 -0.16798324101760187 1104 40-41 -0.17331307014899267 1104 42-43 -0.17127250180963927 1104 44-45 -0.2587021633073121 1104 46-47 -0.20614735098585868 1104 48-49 -0.3459198151574796 1104 50-51 -0.43821616978075895 1104 52-53 -0.3588358883450127 1104 54-55 -0.18030335975609546 1104 56-57 -0.2728532660730494 1104 58-59 -0.29234092530331424 1104 60-61 -0.12563778818529414 1104 62-63 -0.18710785102506833 1104 64-65 -0.2896616568219983 1104 66-67 -0.16458734778671413 1104 68-69 -0.09300910276635221 1104 70-71 -0.19896461693476652 1104 72-73 -0.17904937450468594 1104 74-75 -0.20628853905945022 1104 76-77 -0.12050263094620561 1104 78-79 -0.15577893053184155 1104 80-81 -0.1468116012467533 1104 82-83 -0.035566778300800905 1104 84-85 0.07250108217927576 1104 86-87 0.14781699000587167 1104 88-89 0.08129993675764524 1104 90-91 0.008886606425615895 1104 92-93 -0.11356182791220704 1104 94-95 -0.07315339762305229 1104 96-97 0.2866617538323233 1104 98-99 0.2172372427098601 1104 100-101 0.5431538960082776 1105 1 -0.18289017481149727 1105 2 -0.026510357658665384 1105 3 0.01301663445347856 1105 4 0.0025490578729900903 1105 5 -0.12341400827241245 1105 6 -0.13157405543433498 1105 7 -0.060985856013047623 1105 8 -0.16600615081910064 1105 9 -0.10029802190179282 1105 10-11 -0.16406084720419045 1105 12-13 -0.17884775754943405 1105 14-15 -0.19235477284089342 1105 16-17 -0.2102064531891159 1105 18-19 -0.12499059947652569 1105 20-21 -0.08960914057382041 1105 22-23 -0.11115340995242207 1105 24-25 -0.1466653041097672 1105 26-27 -0.12467043673477463 1105 28-29 -0.19879504600180553 1105 30-31 -0.015456088976982585 1105 32-33 -0.0011001157549586082 1105 34-35 -0.006891000080962328 1105 36-37 -0.16739250732134536 1105 38-39 -0.10557143858013518 1105 40-41 -0.06665665550669075 1105 42-43 -0.3028476403764415 1105 44-45 -0.1944008634383394 1105 46-47 -0.21450000965923977 1105 48-49 -0.09277595239206704 1105 50-51 -0.04789802808352306 1105 52-53 0.021853573999351283 1105 54-55 -0.08708778764379588 1105 56-57 0.05904670267746326 1105 58-59 -0.06594771905122343 1105 60-61 0.05503166352328748 1105 62-63 -0.005426354558458968 1105 64-65 -0.299459467165029 1105 66-67 -0.19525022065612774 1105 68-69 -0.22325970171483078 1105 70-71 -0.30762373986586056 1105 72-73 -0.2963040249216249 1105 74-75 -0.18840122360959555 1105 76-77 -0.18632556149011137 1105 78-79 -0.1967681138237829 1105 80-81 -0.16570218966909067 1105 82-83 -0.17802605802755878 1105 84-85 -0.4092207096772178 1105 86-87 -0.24792650105512948 1105 88-89 -0.08659039399348956 1105 90-91 -0.05014915504760609 1105 92-93 -0.053588307739836694 1105 94-95 -0.05666244078571836 1105 96-97 -0.07754497522366677 1105 98-99 -0.14191419668532745 1105 100-101 0.09838818491799728 1106 1 -0.24763509173032716 1106 2 -0.07827019202719754 1106 3 -0.07640312453150244 1106 4 0.06415122382298222 1106 5 -0.03745552231392679 1106 6 -0.17429848076745458 1106 7 -0.14307429897105095 1106 8 -0.1562062888453255 1106 9 -0.19428857241408792 1106 10-11 -0.3513836693096195 1106 12-13 -0.41924697186168913 1106 14-15 -0.39729451908498703 1106 16-17 -0.24417948498822994 1106 18-19 -0.1598369125837067 1106 20-21 -0.2134956405472721 1106 22-23 -0.17849162729064005 1106 24-25 0.005866946248566762 1106 26-27 -0.025899401007215772 1106 28-29 -0.12135437073504107 1106 30-31 -0.19357627796673427 1106 32-33 -0.05484805863334685 1106 34-35 -0.34308306888172524 1106 36-37 -0.3018433222069419 1106 38-39 -0.0462546683938001 1106 40-41 -0.3617699962722014 1106 42-43 -0.4026087488332024 1106 44-45 -0.3213451751652556 1106 46-47 -0.27200916716839174 1106 48-49 -0.25774648910172715 1106 50-51 -0.3224534243780539 1106 52-53 -0.060396784337960696 1106 54-55 0.052759321768533596 1106 56-57 0.037681835660421825 1106 58-59 -0.18260376831597114 1106 60-61 -0.11617925116588168 1106 62-63 0.046068044762030524 1106 64-65 -0.14826235509835328 1106 66-67 -0.2331410737643722 1106 68-69 -0.1183119924193008 1106 70-71 -0.21081957784431182 1106 72-73 -0.2862626170541276 1106 74-75 -0.4583911370777649 1106 76-77 -0.29288711587775396 1106 78-79 -0.23037484525659835 1106 80-81 -0.3371440328500661 1106 82-83 -0.5432646841357531 1106 84-85 -0.49889794065879656 1106 86-87 -0.29950318524051056 1106 88-89 -0.5817568217686144 1106 90-91 -0.7038785174726208 1106 92-93 -0.5703983093324503 1106 94-95 -1.053264872170761 1106 96-97 -0.650162695667472 1106 98-99 -0.36232084317893154 1106 100-101 0.18535266970857123 1107 1 -0.02609875221980218 1107 2 0.040977102441651425 1107 3 0.0880642802261562 1107 4 0.04189803538886139 1107 5 -0.059864485899360886 1107 6 -0.03687647455343779 1107 7 -0.092131576078053 1107 8 9.949131723914206E-4 1107 9 0.05806717271510564 1107 10-11 0.0844589184838398 1107 12-13 -0.13932398143195002 1107 14-15 -0.14524041129424603 1107 16-17 -0.07743493074590901 1107 18-19 -0.082304079430223 1107 20-21 -0.15783788705153512 1107 22-23 -0.1320614273036682 1107 24-25 -0.10829145725438849 1107 26-27 0.12906288724479253 1107 28-29 -0.19289340090727336 1107 30-31 -0.06923442798103707 1107 32-33 -0.22110900183772486 1107 34-35 -0.17512559266575778 1107 36-37 -0.21227190213233627 1107 38-39 -0.09490112394265537 1107 40-41 0.0264712456239522 1107 42-43 0.024999706075554684 1107 44-45 0.2047684047459768 1107 46-47 0.21308952347065002 1107 48-49 0.1741011741293832 1107 50-51 0.10331971602067114 1107 52-53 0.10222468487661729 1107 54-55 0.18674152941397892 1107 56-57 0.03829360716376584 1107 58-59 0.14120674678997602 1107 60-61 0.3762536168245916 1107 62-63 0.5046084882397182 1107 64-65 0.6072407561562656 1107 66-67 0.46211528965056203 1107 68-69 0.5452859037664766 1107 70-71 0.5928492101966967 1107 72-73 0.34076847849834735 1107 74-75 0.2941387929933512 1107 76-77 0.48231942781151105 1107 78-79 0.4487467528265192 1107 80-81 0.8601586495980413 1107 82-83 0.9983626847086526 1107 84-85 0.7803042356616849 1107 86-87 0.7017876243087926 1107 88-89 0.7626712426826465 1107 90-91 0.9429664692581667 1107 92-93 0.9403416792818007 1107 94-95 0.8522376726287568 1107 96-97 0.5760568113466569 1107 98-99 0.6977177324130857 1107 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44-45 -0.5476772167146962 2316 46-47 -0.49412963928886455 2316 48-49 -0.7563087159248312 2316 50-51 -0.7940518742373754 2316 52-53 -0.6805680784222972 2316 54-55 -0.6771162776722832 2316 56-57 -0.5274205337897726 2316 58-59 -0.6132411663446717 2316 60-61 -0.6196122111205895 2316 62-63 -0.6948850440171483 2316 64-65 -1.0813682990738656 2316 66-67 -0.6931134685193783 2316 68-69 -0.5859377643928951 2316 70-71 -0.7299884122305329 2316 72-73 -0.5599792385968385 2316 74-75 -0.5257231609315305 2316 76-77 -0.5621090372735864 2316 78-79 -0.6565971736528411 2316 80-81 -0.8141352381021392 2316 82-83 -0.8461172261187251 2316 84-85 -0.6592207096772178 2316 86-87 -0.9121715153001446 2316 88-89 -0.7684992259023247 2316 90-91 -0.875362830261281 2316 92-93 -0.7806538348053671 2316 94-95 -0.7795969137201944 2316 96-97 -1.008314205992896 2316 98-99 -1.061857216628347 2316 100-101 -1.3027514162216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 5.0 5 25.0 6 41.0 7 123.0 8 287.0 9 552.0 10 900.0 11 1473.0 12 2357.0 13 3414.0 14 4406.0 15 6183.0 16 8832.0 17 12520.0 18 10987.0 19 6533.0 20 5421.0 21 5413.0 22 5613.0 23 6111.0 24 7089.0 25 8358.0 26 10021.0 27 12437.0 28 15572.0 29 19384.0 30 24521.0 31 31553.0 32 40109.0 33 53306.0 34 73877.0 35 107152.0 36 168618.0 37 274836.0 38 247729.0 39 26572.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43496496513156 20.77467836159979 15.257227501955628 21.533129171313018 2 15.661140493654452 21.736881195735975 39.690054136146294 22.911924174463273 3 19.022754813742214 26.265720399987025 30.314578500738975 24.396946285531783 4 12.892037936056303 17.989601934229864 37.64221238768767 31.476147742026168 5 13.194188527624458 38.59221948165926 34.327117847442615 13.886474143273675 6 30.860431209500817 37.9254103875862 17.34036740054178 13.873791002371211 7 27.6215776083118 33.97045259282116 21.127984703149444 17.2799850957176 8 26.41235125867164 35.6719693863378 19.811384310082264 18.1042950449083 9 26.63475109765216 17.124371745406243 20.80345060752417 35.43742654941743 10-11 24.49945481266099 27.896832756818167 27.428204524680645 20.175507905840202 12-13 25.443439523715263 25.378772126230757 28.19968460608198 20.978103743972003 14-15 22.52101944626266 26.68078636723919 26.612918652726066 24.18527553377209 16-17 21.46204190332344 30.148152891988033 27.154280115674528 21.235525089013997 18-19 21.322896454327772 28.117943441907816 30.277650479606834 20.28150962415758 20-21 22.052224871687603 27.366533065965587 30.591122803136223 19.99011925921059 22-23 21.85153764581124 27.23602187428524 30.315887967063816 20.596552512839704 24-25 21.65238824174723 27.657643833246624 30.29895491161599 20.391013013390154 26-27 21.50472751105359 27.991664476463914 30.170819180187703 20.33278883229479 28-29 21.09965761499923 27.828627012442386 30.53177593141155 20.539939441146835 30-31 21.20495966611746 27.607396915512183 30.500341417853033 20.687302000517324 32-33 21.114469380099955 27.991606360602127 30.182086749244597 20.71183751005332 34-35 21.665229190768994 27.648441911973244 30.186577995057966 20.499750902199796 36-37 21.593826699458308 27.664036515491887 29.908578196332314 20.83355858871749 38-39 21.400245687780235 28.11070754587516 29.92600256666381 20.56304419968079 40-41 21.537727712400578 27.89433762025519 30.14923411783199 20.41870054951224 42-43 21.339151766013824 28.16733927737362 29.705366980089533 20.788141976523022 44-45 21.450065247821122 28.324582334932163 29.494967725408554 20.730384691838157 46-47 21.583846153206377 28.50705998890496 28.87430250536662 21.034791352522042 48-49 21.699662075338153 28.761688675157338 28.743765277513244 20.79488397199126 50-51 21.768611015695242 28.783188435208373 28.488762320776416 20.959438228319964 52-53 22.052058529250072 28.886653431353388 28.23450790500849 20.826780134388056 54-55 21.561681023405214 28.948657574943503 28.53355002208196 20.95611137956932 56-57 21.527123382007904 29.39391469460776 28.20860007036285 20.870361853021485 58-59 21.781377797775836 28.737069994402574 28.384964639756344 21.096587568065246 60-61 21.79223164182481 28.88083144603976 28.621753099583398 20.705183812552033 62-63 21.6931747202744 28.842198414922915 28.829390047232934 20.635236817569755 64-65 21.634123154950476 28.54956048169443 28.779778415238965 21.03653794811613 66-67 21.331629432298215 29.125146901165145 28.38508939658449 21.158134269952154 68-69 21.442288738866495 28.811924091292056 28.614641960378894 21.131145209462556 70-71 21.400536787045915 29.05403550912014 28.419439109934885 21.12598859389906 72-73 21.507266482608223 29.023138716273454 28.266611108130803 21.202983692987523 74-75 21.465743022558527 29.354616293740783 27.938418366201212 21.241222317499474 76-77 21.674045339958198 28.840992432250808 28.282664040674057 21.202298187116945 78-79 21.550660836918706 29.01918676845715 27.93916690717011 21.490985487454036 80-81 21.359658133022382 29.443443155382965 27.992937100102385 21.203961611492268 82-83 21.529173209485535 29.10035131610465 28.092687900886276 21.27778757352354 84-85 21.250882149046074 29.108527309088807 27.844429039036626 21.79616150282849 86-87 21.147238840293795 28.995649313546345 28.145847385803833 21.711264460356023 88-89 21.293828113369027 28.692531806753337 28.18131991060757 21.832320169270066 90-91 21.122654810575657 28.82327742171611 28.312420198054394 21.74164756965384 92-93 21.169688115606448 28.8843948015525 28.087810197031494 21.858106885809566 94-95 20.984680693215473 28.62453933541206 28.179864414279166 22.2109155570933 96-97 20.99470282507679 28.95934507655495 28.058351263661912 21.987600834706353 98-99 20.731058682725372 29.40182818731996 27.752874918336225 22.114238211618442 100-101 20.46699274638983 29.589988021561194 27.79201603779863 22.15100319425035 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 479.0 1 312.0 2 169.5 3 239.5 4 265.0 5 284.0 6 459.5 7 848.0 8 1311.0 9 1607.5 10 1684.5 11 1707.0 12 1831.5 13 2013.0 14 2165.0 15 2307.0 16 2439.5 17 2566.5 18 2648.0 19 2712.5 20 2955.5 21 3401.5 22 3908.5 23 4582.5 24 5377.5 25 6521.0 26 8242.5 27 10261.5 28 11978.0 29 14350.0 30 16978.5 31 19176.5 32 22320.0 33 25876.0 34 30014.0 35 32992.5 36 36500.5 37 40149.5 38 41511.5 39 41903.0 40 41549.5 41 42063.5 42 42751.5 43 44040.0 44 45781.5 45 45623.0 46 43928.0 47 43153.5 48 45288.0 49 45076.5 50 42435.0 51 39974.0 52 36665.5 53 34362.5 54 32094.0 55 28801.0 56 24879.0 57 21579.5 58 19104.5 59 16697.0 60 14362.0 61 11619.5 62 8913.5 63 6732.5 64 4807.0 65 3523.0 66 2582.5 67 2026.0 68 1472.5 69 904.5 70 665.5 71 510.5 72 420.5 73 346.5 74 261.0 75 193.5 76 130.0 77 83.0 78 47.5 79 28.5 80 19.5 81 14.0 82 9.5 83 5.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05647325754217405 2 0.0 3 0.0 4 0.0 5 0.019462065191264696 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0027446502192809185 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001164397062725238 24-25 4.158560938304422E-5 26-27 0.0012475682814913265 28-29 0.003118920703728316 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015178747424811139 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.910992656813095E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.495136562982653E-4 84-85 0.0016218387659387243 86-87 0.0 88-89 0.0 90-91 0.011976655502316734 92-93 0.001538667547172636 94-95 0.0 96-97 0.0 98-99 4.158560938304422E-5 100-101 0.014887648159129829 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1202339.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.46827348975907 #Duplication Level Percentage of deduplicated Percentage of total 1 78.517998628022 42.7673982313963 2 12.051727026125839 13.12873527365886 3 3.820374063431157 6.242675379604512 4 1.7587926564200798 3.8319359768667502 5 0.9265788937761396 2.523457629801859 6 0.5769350147055408 1.8854792500079736 7 0.3836323307707174 1.4627053498353204 8 0.27358269160739307 1.192126149482872 9 0.20768861727170151 1.0181196365638443 >10 1.2941311822671075 13.30895754928598 >50 0.12077902034509871 4.555345050357229 >100 0.0653263080347393 6.0807796929656845 >500 0.0018401754170100744 0.6961890563778663 >1k 3.0669590283501243E-4 0.3773703847288782 >5k 3.0669590283501243E-4 0.9287253890660461 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6095 0.506928578379309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5024 0.41785220308082827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3257 0.27088865952115 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1261 0.10487890686403752 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.6634243753217687E-4 0.0 4 0.0 0.0 0.0 1.6634243753217687E-4 0.0 5 0.0 0.0 0.0 1.6634243753217687E-4 0.0 6 0.0 0.0 0.0 1.6634243753217687E-4 0.0 7 0.0 0.0 0.0 1.6634243753217687E-4 0.0 8 0.0 0.0 0.0 1.6634243753217687E-4 0.0 9 0.0 0.0 0.0 2.495136562982653E-4 0.0 10-11 0.0 0.0 0.0 3.3268487506435374E-4 0.0 12-13 0.0 0.0 0.0 4.1585609383044215E-4 0.0 14-15 0.0 0.0 0.0 4.1585609383044215E-4 0.0 16-17 0.0 0.0 0.0 5.406129219795748E-4 0.0 18-19 0.0 0.0 0.0 8.732977970439286E-4 0.0 20-21 0.0 0.0 0.0 9.564690158100169E-4 0.0 22-23 0.0 0.0 0.0 0.0012891538908743707 0.0 24-25 0.0 0.0 0.0 0.0014970819377895918 0.0 26-27 0.0 0.0 0.0 0.0016634243753217686 0.0 28-29 0.0 0.0 0.0 0.0029109926568130955 0.0 30-31 0.0 0.0 0.0 0.006237841407456633 0.0 32-33 0.0 0.0 0.0 0.009980546251930612 0.0 34-35 0.0 0.0 0.0 0.013848007924553724 0.0 36-37 0.0 0.0 0.0 0.018838281050519032 0.0 38-39 0.0 0.0 0.0 0.02545039294242306 0.0 40-41 0.0 0.0 0.0 0.03185457678741187 0.0 42-43 0.0 0.0 0.0 0.047989793228033026 0.0 44-45 0.0 0.0 0.0 0.06869942670078905 0.0 46-47 0.0 0.0 0.0 0.08791197823575547 0.0 48-49 0.0 0.0 0.0 0.1160654357880764 0.0 50-51 0.0 0.0 0.0 0.15116369010736574 0.0 52-53 0.0 0.0 0.0 0.2085102454465837 0.0 54-55 0.0 0.0 0.0 0.2502206116577771 0.0 56-57 0.0 0.0 0.0 0.3353879396742516 0.0 58-59 0.0 0.0 0.0 0.44629675989883055 0.0 60-61 0.0 0.0 0.0 0.5572055801234095 0.0 62-63 0.0 0.0 0.0 0.7200132408580275 0.0 64-65 0.0 0.0 0.0 0.8274288698944308 0.0 66-67 0.0 0.0 0.0 1.0476246715776498 0.0 68-69 0.0 0.0 0.0 1.2930213525469938 0.0 70-71 0.0 0.0 0.0 1.489887627366325 0.0 72-73 0.0 0.0 0.0 1.8099720627876166 0.0 74-75 0.0 0.0 0.0 2.131345652099782 0.0 76-77 0.0 0.0 0.0 2.373165970662184 0.0 78-79 0.0 0.0 0.0 2.648670632824852 0.0 80-81 0.0 0.0 0.0 3.1163423959465675 0.0 82-83 0.0 0.0 0.0 3.4925674040349683 0.0 84-85 0.0 0.0 0.0 4.024156248778422 0.0 86-87 0.0 0.0 0.0 4.6744720083104685 0.0 88-89 0.0 0.0 0.0 5.57430142414078 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3825 0.0 38.39751 1 GTATCAA 6135 0.0 35.948387 1 GTGTAGC 420 0.0 29.423891 1 ATCAACG 7845 0.0 27.85634 3 TCAACGC 7880 0.0 27.672327 4 TATCAAC 7965 0.0 27.496307 2 CAACGCA 7970 0.0 27.359842 5 AACGCAG 8215 0.0 26.653992 6 GTACATG 5685 0.0 25.834736 1 TACATGG 6090 0.0 23.558567 2 ACATGGG 5980 0.0 23.35637 3 ACGCAGA 9360 0.0 23.140673 7 CGCAGAG 9410 0.0 22.967234 8 GTATAGG 520 0.0 21.937338 1 GCAGAGT 9815 0.0 21.632374 9 GAGTACT 5275 0.0 21.385927 12-13 AGTACTT 5520 0.0 20.95303 12-13 GACGAGT 230 9.026735E-8 20.650997 6 CATGGGG 2480 0.0 20.113926 4 GTACTTT 5550 0.0 20.025887 14-15 >>END_MODULE