FastQCFastQC Report
Thu 26 May 2016
SRR937448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405274
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89820.6391636079511896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66610.47400008823901957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45100.3209338534691455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21320.15171418527632333No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16760.11926499743110597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35850.050.3743061
GTATCAA69850.043.8841741
ATCAACG88050.034.6402363
TATCAAC88850.034.380582
TCAACGC89050.034.200334
CAACGCA89900.033.7172135
AACGCAG91900.033.031596
ACGCAGA103400.029.081177
CGCAGAG103500.028.9153798
GTACATG58800.027.8032631
GCAGAGT108600.026.7701199
TACATGG60100.026.4016862
ACATGGG60100.025.691183
TATCGCG950.00122611225.0020187
GAGTACT69300.023.37240212-13
AGTACTT73350.023.08562512-13
AGAGTAC101100.022.5752310-11
GTGGTAT19400.022.292271
TGGTATC19300.021.6613252
GTACTTT75450.021.56057214-15
Produced by FastQC (version 0.11.5)