FastQCFastQC Report
Thu 26 May 2016
SRR937448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405274
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72680.5171945115329822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59270.4217682814881653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39980.28449967764293654No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.10688307049016776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41900.042.080321
GTATCAA72500.039.0685581
ATCAACG92700.030.335053
TCAACGC93500.030.0754974
TATCAAC94050.029.9501232
CAACGCA94950.029.6662375
AACGCAG98350.028.6858966
CCGAACG350.0083847327.1487436-37
ACGCAGA112250.025.0535347
CGCAGAG113550.024.6830318
CGTATAG1351.3376119E-524.6424221
GCAGAGT117950.023.5608839
GTACATG67000.022.4855541
GAGTACT65300.022.40635312-13
TTACGCG550.002879822421.58905868-69
AGTACTT69250.021.5741912-13
TACATGG71150.021.0967182
GTACTTT69750.020.97311214-15
AGAGTAC107500.020.76935610-11
ACATGGG69950.020.3721223
Produced by FastQC (version 0.11.5)