##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937448_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1405274 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2570651702088 33.0 31.0 34.0 31.0 34.0 2 32.465614534959016 34.0 31.0 34.0 31.0 34.0 3 32.513044431192775 34.0 31.0 34.0 31.0 34.0 4 36.14456468987542 37.0 35.0 37.0 35.0 37.0 5 36.06990594005155 37.0 35.0 37.0 35.0 37.0 6 36.11404039354603 37.0 35.0 37.0 35.0 37.0 7 36.05477579461372 37.0 35.0 37.0 35.0 37.0 8 36.03203076410721 37.0 35.0 37.0 35.0 37.0 9 37.74170019512209 39.0 37.0 39.0 35.0 39.0 10-11 37.66817894588529 39.0 37.0 39.0 35.0 39.0 12-13 37.57530986839578 39.0 37.0 39.0 35.0 39.0 14-15 38.78950759780655 40.0 38.0 41.0 35.0 41.0 16-17 38.76255520275761 40.0 38.0 41.0 35.0 41.0 18-19 38.759042364691865 40.0 38.0 41.0 35.0 41.0 20-21 38.67049699916173 40.0 38.0 41.0 34.0 41.0 22-23 38.519818554957965 40.0 38.0 41.0 34.0 41.0 24-25 38.46222907418767 40.0 38.0 41.0 34.0 41.0 26-27 38.41493153648328 40.0 38.0 41.0 34.0 41.0 28-29 38.28921370494295 40.0 38.0 41.0 34.0 41.0 30-31 38.36909101000944 40.0 38.0 41.0 34.0 41.0 32-33 38.320727843822624 40.0 38.0 41.0 34.0 41.0 34-35 38.20066905101781 40.0 38.0 41.0 34.0 41.0 36-37 38.04145348166976 40.0 37.5 41.0 33.5 41.0 38-39 37.91896704841903 40.0 37.0 41.0 33.0 41.0 40-41 37.749464873042555 40.0 37.0 41.0 33.0 41.0 42-43 37.53750798776609 40.0 37.0 41.0 33.0 41.0 44-45 37.248030633171894 40.0 37.0 41.0 32.5 41.0 46-47 36.88350777143817 40.0 36.5 41.0 31.0 41.0 48-49 36.555665300859474 40.0 36.0 41.0 30.5 41.0 50-51 36.32091250531925 39.0 36.0 41.0 30.0 41.0 52-53 36.0856402381315 39.0 35.5 41.0 29.5 41.0 54-55 35.859752617638975 39.0 35.0 40.5 29.0 41.0 56-57 35.60750074362723 39.0 35.0 40.0 28.0 41.0 58-59 35.351011973465674 38.0 35.0 40.0 28.0 41.0 60-61 35.03774637543995 38.0 34.5 40.0 27.0 41.0 62-63 34.68237795618506 38.0 34.0 40.0 26.5 41.0 64-65 34.29584799832631 37.0 34.0 40.0 26.0 41.0 66-67 33.899120385063696 37.0 33.5 39.0 26.0 41.0 68-69 33.46100262297602 36.0 33.0 39.0 25.0 40.0 70-71 33.06901785701579 36.0 33.0 39.0 24.0 40.0 72-73 32.59766707417913 35.0 32.0 38.0 22.5 40.0 74-75 32.11723976961077 35.0 32.0 37.0 21.5 39.0 76-77 30.848460157947844 34.0 30.5 36.0 20.5 38.5 78-79 31.438096769740277 34.5 31.0 36.0 20.5 39.0 80-81 31.235298952375125 35.0 31.0 36.0 20.0 37.5 82-83 30.88781796290261 34.0 31.0 35.5 18.5 37.0 84-85 30.52670689132511 34.0 31.0 35.0 17.5 37.0 86-87 30.136791116892507 34.0 30.5 35.0 13.0 36.0 88-89 29.696182025711714 34.0 30.0 35.0 7.0 36.0 90-91 29.224650495205918 34.0 29.0 35.0 2.0 35.5 92-93 28.890807771295847 34.0 29.0 35.0 2.0 35.0 94-95 28.480334084313807 34.0 29.0 35.0 2.0 35.0 96-97 28.03218304757649 34.0 29.0 35.0 2.0 35.0 98-99 27.334370023212557 33.0 27.5 35.0 2.0 35.0 100-101 24.891034417487266 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.00458976325255378 1101 2 0.06502121665025129 1101 3 0.0063816093542499175 1101 4 0.05615004897293119 1101 5 0.0422797739290246 1101 6 0.03848540939885936 1101 7 0.005815184387387262 1101 8 -0.014209279551927523 1101 9 0.04937557167405515 1101 10-11 -0.021646117464335646 1101 12-13 -0.017094108225627735 1101 14-15 -0.06990038890186412 1101 16-17 0.039653520108878126 1101 18-19 0.08305912436610896 1101 20-21 0.08651926219155825 1101 22-23 -0.03319017595146789 1101 24-25 -0.02161758660724189 1101 26-27 -0.0203035567103953 1101 28-29 -0.0587793602705986 1101 30-31 -0.03214192415665451 1101 32-33 0.0437131519315912 1101 34-35 0.11082670160642039 1101 36-37 0.10710157544097143 1101 38-39 0.2220565957972198 1101 40-41 0.22763296586053627 1101 42-43 0.1478443606153661 1101 44-45 0.03738732834636238 1101 46-47 0.026663599725957 1101 48-49 0.0054869606877687715 1101 50-51 0.10575227600786263 1101 52-53 0.05264224851407562 1101 54-55 0.1635563436519547 1101 56-57 0.1682318243364591 1101 58-59 0.11409725102936363 1101 60-61 0.11706524656619166 1101 62-63 0.13755850509836165 1101 64-65 0.09601269884664987 1101 66-67 0.13066528232621266 1101 68-69 0.06732068183507778 1101 70-71 0.06553165644354664 1101 72-73 0.14448701699078725 1101 74-75 0.04922129942470832 1101 76-77 0.04656099085939047 1101 78-79 -0.03341133430339127 1101 80-81 0.10716526351675881 1101 82-83 0.10230633789231902 1101 84-85 0.1130289006684535 1101 86-87 -0.027346514038885772 1101 88-89 0.2465026246211437 1101 90-91 0.3289570044907961 1101 92-93 0.2669942004327588 1101 94-95 0.12300340642817886 1101 96-97 0.16385431321183574 1101 98-99 0.4610240726950998 1101 100-101 0.9509215670180886 1102 1 -0.047183300000213535 1102 2 0.020233921884781125 1102 3 0.03053105222914354 1102 4 0.019478601694757458 1102 5 0.001175788433947389 1102 6 0.009664557618606295 1102 7 0.011924614023662627 1102 8 -0.056864965201938844 1102 9 -0.10280712123321223 1102 10-11 -0.10389222142585197 1102 12-13 -0.14175271020854296 1102 14-15 0.009015675391765399 1102 16-17 -0.03152059168068888 1102 18-19 -0.17616905470057276 1102 20-21 -0.04893600711356072 1102 22-23 -0.016862069313447137 1102 24-25 -0.02522247146485057 1102 26-27 -0.14484967841932672 1102 28-29 -0.19869690671368545 1102 30-31 -0.21606396021749674 1102 32-33 -0.12642321541124346 1102 34-35 -0.2276698520363567 1102 36-37 -0.1447482334145107 1102 38-39 -0.09308794974888457 1102 40-41 0.006544473384010985 1102 42-43 -0.22401134805832612 1102 44-45 -0.2979844801157938 1102 46-47 -0.3476765080056836 1102 48-49 -0.19076137848941954 1102 50-51 -0.2547095886224824 1102 52-53 -0.20832325913410443 1102 54-55 -0.13748643722496467 1102 56-57 -0.30642461917637576 1102 58-59 -0.2459777677972852 1102 60-61 -0.21555834982867594 1102 62-63 -0.23885301441660545 1102 64-65 -0.30463100489613737 1102 66-67 -0.285699038643088 1102 68-69 -0.1927202811303701 1102 70-71 -0.14264678667597508 1102 72-73 -0.19995444290243825 1102 74-75 -0.1220002987065385 1102 76-77 -0.17247215844438202 1102 78-79 -0.10440797188667617 1102 80-81 -0.19038871022665305 1102 82-83 -0.03339643598155817 1102 84-85 -0.11160355984353387 1102 86-87 -0.1284557278281504 1102 88-89 -0.059579704224169916 1102 90-91 -0.40073586920032866 1102 92-93 -0.22940571130979848 1102 94-95 -0.28539424131909996 1102 96-97 -0.2469744913315033 1102 98-99 -0.018325124593861375 1102 100-101 0.6764536298919204 1103 1 0.002128241242289164 1103 2 0.010926854193705537 1103 3 0.023887308178707656 1103 4 0.019919714672738564 1103 5 0.027527607244707042 1103 6 -0.06801346111829787 1103 7 -0.0541913549943942 1103 8 -0.05923230619579556 1103 9 -0.05298580320011581 1103 10-11 -0.05723073922382582 1103 12-13 -0.0851404370722193 1103 14-15 -0.13845156624763177 1103 16-17 -0.09404393477606732 1103 18-19 -0.14602642123528398 1103 20-21 -0.12678308447998887 1103 22-23 -0.12639307466611172 1103 24-25 -0.1527964541220328 1103 26-27 -0.284395873039621 1103 28-29 -0.25803180405608117 1103 30-31 -0.22736097802327038 1103 32-33 -0.2200208350657391 1103 34-35 -0.24379492421139304 1103 36-37 -0.31477491866895946 1103 38-39 -0.19001108269139166 1103 40-41 -0.275304871968963 1103 42-43 -0.2833139942160159 1103 44-45 -0.3754242562301542 1103 46-47 -0.16723086945823695 1103 48-49 -0.1735314431478443 1103 50-51 -0.17086717207242685 1103 52-53 -0.24975120634620396 1103 54-55 -0.18981164360653935 1103 56-57 -0.17619823666692014 1103 58-59 -0.20974044069224362 1103 60-61 -0.05022057068687502 1103 62-63 -0.05028163786321471 1103 64-65 -0.2493119002306372 1103 66-67 -0.061853448992593485 1103 68-69 -0.2414021440004106 1103 70-71 -0.12648608214363577 1103 72-73 0.0972636323260545 1103 74-75 0.1765361737421074 1103 76-77 0.28472609791092296 1103 78-79 0.0048738670550712015 1103 80-81 -0.0201123383808941 1103 82-83 0.06041393745595158 1103 84-85 -0.06799501640066552 1103 86-87 0.15356969858973102 1103 88-89 0.27625578352298064 1103 90-91 0.20038772046056508 1103 92-93 0.21079278953029146 1103 94-95 0.35928561365460254 1103 96-97 0.5002412430851919 1103 98-99 0.5887823602818933 1103 100-101 1.2686093094816009 1104 1 0.07047993429279131 1104 2 0.08174380894342903 1104 3 0.04730126187016026 1104 4 0.013719503939114475 1104 5 0.09507095184245173 1104 6 0.03219761721968695 1104 7 0.062191288552085666 1104 8 0.03621543381819947 1104 9 0.10956175262303702 1104 10-11 -0.02471266772376879 1104 12-13 0.09252903746106966 1104 14-15 0.05512490742273002 1104 16-17 -0.07043952798433395 1104 18-19 -0.04920356962094985 1104 20-21 -0.011286923991512765 1104 22-23 -0.23459686350503972 1104 24-25 0.020351267392442196 1104 26-27 -0.1666352452970088 1104 28-29 -0.10491296936477568 1104 30-31 -0.1566009006700071 1104 32-33 -0.08979687600827191 1104 34-35 -0.06780879080573499 1104 36-37 -0.06743379332382204 1104 38-39 -0.1346848879500797 1104 40-41 -0.1362336051657138 1104 42-43 -0.03812070873630091 1104 44-45 -0.13276661104628573 1104 46-47 -0.15706532750857605 1104 48-49 -0.09813499620411648 1104 50-51 -0.08365918579654164 1104 52-53 -0.07213458757527746 1104 54-55 0.0582507905392049 1104 56-57 0.06514519594715296 1104 58-59 -0.10492918747063129 1104 60-61 0.04006138033421536 1104 62-63 0.1070696794517545 1104 64-65 0.05642593748608249 1104 66-67 -0.026829899452380346 1104 68-69 0.021646909594181807 1104 70-71 0.13548804610972098 1104 72-73 0.04821635744276165 1104 74-75 0.11711107208093807 1104 76-77 0.17537729239370137 1104 78-79 0.017128073397074672 1104 80-81 -0.017051874835861014 1104 82-83 0.07984187724174419 1104 84-85 0.026230721368108334 1104 86-87 -0.050341800181371354 1104 88-89 0.06988448098464062 1104 90-91 -0.14845801310272932 1104 92-93 0.1784597131985315 1104 94-95 0.4209223268075313 1104 96-97 0.1699249013474109 1104 98-99 0.43533863500775993 1104 100-101 0.9598828231143663 1105 1 -0.03553562135746091 1105 2 -0.05504424157071952 1105 3 -0.04009678884204959 1105 4 0.011340062029127296 1105 5 -0.026282986315464996 1105 6 -0.04585972105838465 1105 7 -0.0868048684496685 1105 8 -0.0698592402651812 1105 9 -0.15940221034061608 1105 10-11 -0.045886819824389136 1105 12-13 -0.02620028234084515 1105 14-15 -0.09625849392181607 1105 16-17 -0.13742431384056175 1105 18-19 -0.10391692924647344 1105 20-21 -0.15670746611654351 1105 22-23 -0.19454337482726913 1105 24-25 -0.2793193198966719 1105 26-27 -0.07869432531816045 1105 28-29 -0.11782884653527503 1105 30-31 -0.1310267766581461 1105 32-33 -0.13577331146084504 1105 34-35 -0.14546280332684347 1105 36-37 -0.10050740567614724 1105 38-39 -0.25106957669603247 1105 40-41 -0.18555473349353235 1105 42-43 -0.17564038737209842 1105 44-45 -0.13429978909120877 1105 46-47 -0.05286413757755071 1105 48-49 -0.02776995638469515 1105 50-51 -0.05308340226723374 1105 52-53 -0.11530349764143466 1105 54-55 0.10168468322694224 1105 56-57 -0.07544053868490863 1105 58-59 -0.10684183383376933 1105 60-61 -0.004612498981352076 1105 62-63 0.06125064307621386 1105 64-65 0.05839275073844874 1105 66-67 0.13001220391544877 1105 68-69 0.1904168320560089 1105 70-71 0.2007210357558904 1105 72-73 0.1732125312497601 1105 74-75 0.275291292673721 1105 76-77 0.28456228627479163 1105 78-79 0.2223299553438629 1105 80-81 0.08703678000672 1105 82-83 0.09278326236562862 1105 84-85 0.17515110474364093 1105 86-87 0.08303313612377039 1105 88-89 0.31820335681012324 1105 90-91 0.4375099417055779 1105 92-93 0.5619650401594747 1105 94-95 0.9250837243312766 1105 96-97 0.6174814494791185 1105 98-99 0.7873246842579178 1105 100-101 1.166690805843384 1106 1 -0.26037240125101135 1106 2 -0.1260022945726078 1106 3 -0.13045161988872422 1106 4 -0.03760506930311891 1106 5 -0.17894404074573345 1106 6 -0.07258020592515635 1106 7 -0.13578670327781595 1106 8 -0.11011549863604131 1106 9 -0.13642242343159694 1106 10-11 -0.29424017525459334 1106 12-13 -0.2663765746692377 1106 14-15 -0.3943758424748651 1106 16-17 -0.24869792854697437 1106 18-19 -0.2601584436870965 1106 20-21 -0.2528568615793816 1106 22-23 -0.24383532021110454 1106 24-25 -0.2989980011808413 1106 26-27 -0.2013504417688452 1106 28-29 -0.3756965637375558 1106 30-31 -0.1959437061116489 1106 32-33 -0.1747971140177853 1106 34-35 -0.02410320388433007 1106 36-37 -0.10438829059743426 1106 38-39 -0.21419615719784701 1106 40-41 -0.22804208862942232 1106 42-43 -0.17181098929923166 1106 44-45 0.09175279410423798 1106 46-47 -0.1494349435489397 1106 48-49 -0.21475999444418647 1106 50-51 -0.06349190198125854 1106 52-53 -0.06429787283069288 1106 54-55 -0.0050193462391661114 1106 56-57 -0.09467283069248111 1106 58-59 -0.098226537787788 1106 60-61 -0.026941818379590643 1106 62-63 0.15334124361677226 1106 64-65 0.07082283142837298 1106 66-67 0.004378176512211951 1106 68-69 0.046432416088485695 1106 70-71 -0.002528141219194424 1106 72-73 -0.03335151516112944 1106 74-75 0.07965843094632419 1106 76-77 0.2148662557918577 1106 78-79 0.08543781026581954 1106 80-81 0.11827128509915852 1106 82-83 0.04711204888348064 1106 84-85 0.030936102461570414 1106 86-87 0.18912544065811332 1106 88-89 0.3578932598483249 1106 90-91 0.2266468284130987 1106 92-93 0.3405289411157746 1106 94-95 0.504091567664446 1106 96-97 0.38478145535847474 1106 98-99 0.41001274634392004 1106 100-101 0.9099806081508781 1107 1 0.01798728930830862 1107 2 -0.003688912019903512 1107 3 0.010930966446359491 1107 4 0.04431431806102637 1107 5 0.0584655645984995 1107 6 -0.01806536744949483 1107 7 -0.10913762010572725 1107 8 -0.07348441932895611 1107 9 -0.07330680594947125 1107 10-11 -0.03368557454043497 1107 12-13 0.058906664921487106 1107 14-15 -0.0805729193389979 1107 16-17 -0.03145529433771088 1107 18-19 -0.10187156128632324 1107 20-21 -0.05446003429517532 1107 22-23 0.16865136305418105 1107 24-25 0.03130566240751165 1107 26-27 -0.12084890381358804 1107 28-29 -0.06249657190475233 1107 30-31 -0.05837087693458187 1107 32-33 0.03821539260304263 1107 34-35 -0.03635279822686499 1107 36-37 0.052104210594613676 1107 38-39 -0.07189416067851795 1107 40-41 -0.2305504990406746 1107 42-43 -0.26416697339242035 1107 44-45 -0.1467137426079148 1107 46-47 -0.1780111248799301 1107 48-49 -0.348025227767792 1107 50-51 -0.2233671840798408 1107 52-53 -0.30843880982436644 1107 54-55 -0.484596549670826 1107 56-57 -0.19848571060226305 1107 58-59 -0.3891090634694194 1107 60-61 -0.375471542772722 1107 62-63 -0.3605171684159032 1107 64-65 -0.3513275675031693 1107 66-67 -0.3696482155444727 1107 68-69 -0.14774929683694182 1107 70-71 -0.14455209272525593 1107 72-73 -0.13646718068559238 1107 74-75 -0.014378434339363366 1107 76-77 0.04310349845009043 1107 78-79 -0.177848597716892 1107 80-81 0.031891767468223975 1107 82-83 -0.018550412877821998 1107 84-85 0.007603141456328899 1107 86-87 0.07739878713102044 1107 88-89 0.20902507720578 1107 90-91 0.09307832785040304 1107 92-93 0.19335905501213801 1107 94-95 0.021048109769349566 1107 96-97 0.20454703278530317 1107 98-99 0.1893753206443911 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66-67 1.337438708237812 2315 68-69 1.3615175532234858 2315 70-71 1.3454925715487818 2315 72-73 1.3684471039876698 2315 74-75 1.6475637176831341 2315 76-77 1.6036834052957794 2315 78-79 1.4357006787279367 2315 80-81 1.589719249812024 2315 82-83 1.5160522542260715 2315 84-85 1.3145151731798066 2315 86-87 1.5160461367268887 2315 88-89 1.5317996061206571 2315 90-91 1.8295758107407387 2315 92-93 1.69282162628976 2315 94-95 1.5761003952077637 2315 96-97 1.6899931897144889 2315 98-99 1.8556654835733504 2315 100-101 2.0645043642043355 2316 1 -0.5415083765703379 2316 2 -0.3461881862101066 2316 3 -0.26291361348258135 2316 4 0.0033924704949086504 2316 5 0.06804625391043118 2316 6 -0.06689508555879797 2316 7 -0.0147823465791177 2316 8 0.01366805602807375 2316 9 0.04679052168798137 2316 10-11 0.17126237452844606 2316 12-13 0.02798025512236535 2316 14-15 0.15552955117930622 2316 16-17 0.20829005011088242 2316 18-19 0.09321919950058799 2316 20-21 0.32761315498219545 2316 22-23 0.3496130264719852 2316 24-25 0.4155355436511954 2316 26-27 0.3171185522576323 2316 28-29 0.421155426491552 2316 30-31 0.38408277492556664 2316 32-33 0.21751114972635577 2316 34-35 0.28247816412780224 2316 36-37 0.05981161938451862 2316 38-39 0.2759346311592381 2316 40-41 0.21334785993794014 2316 42-43 0.34133120862685473 2316 44-45 0.5480175695179454 2316 46-47 0.6866426292848331 2316 48-49 0.3939804957608928 2316 50-51 0.25217648202000476 2316 52-53 0.5259452195951226 2316 54-55 0.6341459330134214 2316 56-57 0.5197134516058597 2316 58-59 0.3819083144908433 2316 60-61 0.6583926685830832 2316 62-63 0.35232482624765993 2316 64-65 0.41582573735077233 2316 66-67 0.34356705272865895 2316 68-69 0.44306678987313575 2316 70-71 0.7875812350781004 2316 72-73 0.6044394594371738 2316 74-75 0.5206332477421185 2316 76-77 0.2269269262078204 2316 78-79 0.1491736466252469 2316 80-81 0.3714601525759882 2316 82-83 0.2148078053628133 2316 84-85 0.2633093889505602 2316 86-87 0.23447093953725684 2316 88-89 0.4508303389811772 2316 90-91 0.4486352659008084 2316 92-93 0.7368661679481434 2316 94-95 0.6221637831134679 2316 96-97 1.0667883334364596 2316 98-99 1.3540857570980158 2316 100-101 0.6850993576865854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 15.0 5 32.0 6 51.0 7 149.0 8 302.0 9 596.0 10 1087.0 11 1658.0 12 2626.0 13 3987.0 14 5083.0 15 7098.0 16 9976.0 17 14968.0 18 13333.0 19 7671.0 20 6751.0 21 6714.0 22 6774.0 23 7512.0 24 8495.0 25 10099.0 26 12162.0 27 15001.0 28 18404.0 29 23391.0 30 29229.0 31 36742.0 32 47607.0 33 63498.0 34 88328.0 35 127764.0 36 199243.0 37 319828.0 38 281170.0 39 27923.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.463971312342146 20.692454322837264 15.23704273882824 21.60653162599235 2 15.642358714385947 21.660900294177505 39.60964196306201 23.08709902837454 3 18.931254687697916 26.116757301423064 30.340204116777226 24.61178389410179 4 12.792807665978307 17.820368127496845 37.71378393110525 31.673040275419602 5 13.225517670316345 38.42018220401099 34.36805722589341 13.986242899779256 6 30.808226723044758 37.926126862092374 17.3812366840915 13.884409730771367 7 27.797924105903903 33.844004799064095 20.98437742390452 17.373693671127484 8 26.476260145708242 35.55740730989116 19.759634064246544 18.20669848015405 9 26.603139316603023 17.000029887409855 20.763424072458466 35.63340672352865 10-11 24.556197543462275 27.84296688786963 27.392057258727053 20.20877830994104 12-13 25.518483581634943 25.251684927085826 28.14156483210864 21.08826665917059 14-15 22.5215509573222 26.6002928966166 26.64056973942448 24.237586406636712 16-17 21.508225442155766 30.05196851290211 27.119124099641777 21.320681945300347 18-19 21.32879424226165 28.023182667579416 30.261642925151968 20.38638016500697 20-21 22.05352835105467 27.264931963446276 30.500564302762307 20.180975382736747 22-23 21.8560945019169 27.12247711726457 30.25893738714297 20.76249099367556 24-25 21.705229974417637 27.47826922324259 30.324316054267346 20.49218474807243 26-27 21.526590282714555 27.80353797031031 30.18052059783572 20.489351149139416 28-29 21.109883947658318 27.68842493887113 30.525010887912853 20.676680225557703 30-31 21.279840372199203 27.483350162851384 30.483345181598683 20.75346428335073 32-33 21.23155341947549 27.74971286738387 30.203825019177756 20.814908693962884 34-35 21.686019950557686 27.56323677802336 30.143231853716784 20.60751141770217 36-37 21.62358430583086 27.497351938964194 29.93936767469108 20.939696080513865 38-39 21.413099014706113 27.90251098368565 30.02079321637327 20.663596785234965 40-41 21.548581112502298 27.720760407137828 30.15551776931679 20.575140711043083 42-43 21.43698472978205 27.93579476872623 29.71603913776219 20.91118136372953 44-45 21.515806732008727 28.065359612003803 29.511165635964296 20.907668020023177 46-47 21.63471323030242 28.28409263958488 28.97007985631272 21.111114273799984 48-49 21.76500810518091 28.505722015777707 28.799294657127366 20.929975221914017 50-51 21.81884102317413 28.5839629851545 28.469181099202007 21.12801489246937 52-53 22.05868748728006 28.701057587345957 28.272564638639867 20.96769028673412 54-55 21.576610682329566 28.68949400615111 28.562436934007174 21.171458377512142 56-57 21.575187472336353 29.151503550197326 28.234636092320788 21.03867288514553 58-59 21.787067860075688 28.54784903157676 28.47120917344233 21.193873934905223 60-61 21.82147396166157 28.597625801089325 28.699669957602573 20.88123027964653 62-63 21.735013954573983 28.56314853900378 28.862093798077815 20.83974370834442 64-65 21.54409799942858 28.370064617314704 28.814426515550174 21.271410867706535 66-67 21.42439837355562 28.87319483602486 28.44313635632624 21.25927043409328 68-69 21.528470604309195 28.586275701393465 28.608050814289598 21.277202880007742 70-71 21.46204227787606 28.821959276269254 28.451070752038394 21.264927693816297 72-73 21.594009424496573 28.780188062968502 28.2566246867159 21.369177825819023 74-75 21.539287000257602 29.023307910058822 27.980308466533927 21.45709662314965 76-77 21.63599411929631 28.655728349062176 28.265519749173475 21.442757782468043 78-79 21.639267502280692 28.741014207905362 28.008061061401552 21.611657228412394 80-81 21.44583294491533 29.28338479427129 27.870207425888065 21.40057483492531 82-83 21.599715641530427 29.0349495487873 27.937187804323514 21.428147005358763 84-85 21.399611533311962 28.952865591166386 27.720218591678385 21.927304283843267 86-87 21.254573841115683 28.77566937124006 28.107329958428036 21.862426829216226 88-89 21.39671693918766 28.510632090254283 28.07409800508655 22.018552965471503 90-91 21.233281287139846 28.705800469981398 28.17949104800728 21.88142719487148 92-93 21.294178888494727 28.701462420791916 28.00698956084679 21.997369129866566 94-95 21.053011725827133 28.517036535223735 28.111919810656143 22.31803192829299 96-97 21.197467540138078 28.651672200581523 27.946364908195843 22.204495351084557 98-99 20.83958805142775 29.106667923836838 27.689825282996715 22.363918741738704 100-101 20.718619339916376 29.20455475491504 27.827239569433328 22.249586335735255 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 447.0 1 308.0 2 204.5 3 252.0 4 254.0 5 293.5 6 501.0 7 943.0 8 1502.0 9 1870.0 10 1888.5 11 1884.0 12 2035.0 13 2194.5 14 2435.5 15 2636.0 16 2751.5 17 2915.0 18 3043.0 19 3157.0 20 3394.5 21 3869.0 22 4461.0 23 5184.0 24 6140.5 25 7428.0 26 9335.5 27 11623.0 28 13548.5 29 16176.5 30 19364.5 31 22071.0 32 25451.0 33 29484.5 34 34281.5 35 37792.5 36 41933.5 37 46275.5 38 48195.5 39 48953.0 40 48266.0 41 48702.5 42 49943.0 43 51454.0 44 53241.0 45 53341.5 46 51504.5 47 50607.0 48 53082.5 49 53027.0 50 50287.5 51 47422.5 52 43148.5 53 40715.0 54 38466.5 55 34296.5 56 29881.0 57 25832.5 58 22815.5 59 20100.0 60 17187.0 61 14046.5 62 10920.5 63 8267.5 64 5881.0 65 4357.5 66 3268.5 67 2475.0 68 1801.0 69 1158.0 70 842.0 71 643.0 72 529.5 73 443.0 74 332.5 75 253.5 76 172.5 77 95.5 78 54.5 79 43.0 80 35.0 81 20.5 82 19.5 83 19.0 84 9.5 85 4.5 86 2.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06454257319213193 2 0.0 3 0.0 4 0.0 5 0.0025617779877803192 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.4906174881197545E-4 12-13 0.0067246672179233375 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.183457460964907E-4 24-25 6.404444969450798E-4 26-27 3.558024983028221E-4 28-29 0.003273382984385963 30-31 3.558024983028221E-4 32-33 0.0 34-35 0.0 36-37 3.558024983028221E-5 38-39 5.692839972845153E-4 40-41 3.558024983028221E-5 42-43 0.01323585293686498 44-45 1.7790124915141104E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.558024983028221E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.116049966056442E-5 82-83 3.913827481331043E-4 84-85 3.913827481331043E-4 86-87 0.0 88-89 0.0 90-91 0.007969975961983214 92-93 0.002526197737950037 94-95 0.0 96-97 0.0 98-99 1.0674074949084661E-4 100-101 0.010602914449424098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1405274.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.11096412041145 #Duplication Level Percentage of deduplicated Percentage of total 1 77.67793179482996 41.255498485029825 2 12.470669192047769 13.246585280327391 3 4.019015595533151 6.403613792812059 4 1.8172963294515498 3.860734405986268 5 0.9714709644920149 2.579787976957846 6 0.5948043430056154 1.895437927202169 7 0.4111589853634859 1.5285935083597415 8 0.29977103941513344 1.273690313497248 9 0.2189241529513332 1.0464545549240751 >10 1.3141240977077029 13.28446736558298 >50 0.13001225762971722 4.782185571054312 >100 0.07172614648903972 6.618123257005583 >500 0.002287683409510175 0.7409421223807129 >1k 5.382784492965118E-4 0.5408227105290686 >5k 2.691392246482559E-4 0.9430627283508736 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7268 0.5171945115329822 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5927 0.4217682814881653 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3998 0.28449967764293654 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1502 0.10688307049016776 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.116049966056442E-5 0.0 3 0.0 0.0 0.0 7.116049966056442E-5 0.0 4 0.0 0.0 0.0 7.116049966056442E-5 0.0 5 0.0 0.0 0.0 7.116049966056442E-5 0.0 6 0.0 0.0 0.0 7.116049966056442E-5 0.0 7 0.0 0.0 0.0 7.116049966056442E-5 0.0 8 0.0 0.0 0.0 7.116049966056442E-5 0.0 9 0.0 0.0 0.0 7.116049966056442E-5 0.0 10-11 0.0 0.0 0.0 2.1348149898169324E-4 0.0 12-13 0.0 0.0 0.0 4.269629979633865E-4 0.0 14-15 0.0 0.0 0.0 4.981234976239509E-4 0.0 16-17 0.0 0.0 0.0 5.692839972845152E-4 0.0 18-19 0.0 0.0 0.0 9.606667454176196E-4 0.0 20-21 0.0 0.0 0.0 0.001031827245078184 0.0 22-23 0.0 0.0 0.0 0.0013520494935507239 0.0 24-25 0.0 0.0 0.0 0.0014943704928718528 0.0 26-27 0.0 0.0 0.0 0.001707851991853546 0.0 28-29 0.0 0.0 0.0 0.002917580486083141 0.0 30-31 0.0 0.0 0.0 0.005443778224033178 0.0 32-33 0.0 0.0 0.0 0.00836135871011632 0.0 34-35 0.0 0.0 0.0 0.011563581194841719 0.0 36-37 0.0 0.0 0.0 0.016687137170402357 0.0 38-39 0.0 0.0 0.0 0.02334064388866513 0.0 40-41 0.0 0.0 0.0 0.029887409857437056 0.0 42-43 0.0 0.0 0.0 0.04568504078208235 0.0 44-45 0.0 0.0 0.0 0.0648272151907742 0.0 46-47 0.0 0.0 0.0 0.08692255033537943 0.0 48-49 0.0 0.0 0.0 0.11286055246165516 0.0 50-51 0.0 0.0 0.0 0.14449139456077603 0.0 52-53 0.0 0.0 0.0 0.20504897977191638 0.0 54-55 0.0 0.0 0.0 0.25407856403804524 0.0 56-57 0.0 0.0 0.0 0.3425310651161268 0.0 58-59 0.0 0.0 0.0 0.45677924732116304 0.0 60-61 0.0 0.0 0.0 0.5691060960353639 0.0 62-63 0.0 0.0 0.0 0.7277940102784226 0.0 64-65 0.0 0.0 0.0 0.8402987602417749 0.0 66-67 0.0 0.0 0.0 1.035242949061891 3.558024983028221E-5 68-69 0.0 0.0 0.0 1.2659808692112713 7.116049966056442E-5 70-71 0.0 0.0 0.0 1.4752638987129911 7.116049966056442E-5 72-73 0.0 0.0 0.0 1.7813252077530788 7.116049966056442E-5 74-75 0.0 0.0 0.0 2.0745064663546042 7.116049966056442E-5 76-77 0.0 0.0 0.0 2.3084110287388793 7.116049966056442E-5 78-79 0.0 0.0 0.0 2.5754408037151473 7.116049966056442E-5 80-81 0.0 0.0 0.0 3.0022614806792127 7.116049966056442E-5 82-83 0.0 0.0 0.0 3.3822585488666266 1.4232099932112884E-4 84-85 0.0 0.0 0.0 3.88447377522106 1.4232099932112884E-4 86-87 0.0 0.0 0.0 4.4904410100805965 1.4232099932112884E-4 88-89 0.0 0.0 0.0 5.273064185347484 1.4232099932112884E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4190 0.0 42.08032 1 GTATCAA 7250 0.0 39.068558 1 ATCAACG 9270 0.0 30.33505 3 TCAACGC 9350 0.0 30.075497 4 TATCAAC 9405 0.0 29.950123 2 CAACGCA 9495 0.0 29.666237 5 AACGCAG 9835 0.0 28.685896 6 CCGAACG 35 0.00838473 27.14874 36-37 ACGCAGA 11225 0.0 25.053534 7 CGCAGAG 11355 0.0 24.683031 8 CGTATAG 135 1.3376119E-5 24.642422 1 GCAGAGT 11795 0.0 23.560883 9 GTACATG 6700 0.0 22.485554 1 GAGTACT 6530 0.0 22.406353 12-13 TTACGCG 55 0.0028798224 21.589058 68-69 AGTACTT 6925 0.0 21.57419 12-13 TACATGG 7115 0.0 21.096718 2 GTACTTT 6975 0.0 20.973112 14-15 AGAGTAC 10750 0.0 20.769356 10-11 ACATGGG 6995 0.0 20.372122 3 >>END_MODULE