FastQCFastQC Report
Thu 26 May 2016
SRR937447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215822
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72290.3262446171217724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54420.24559734491308416No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36970.16684553181618378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.15420913773759806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38700.050.3645941
GTATCAA69100.039.833911
ATCAACG82800.032.6489223
TATCAAC84200.032.1639482
TCAACGC84200.032.1067924
CAACGCA87100.031.0392025
AACGCAG88650.030.4909736
ACGCAGA102100.026.289377
GTACATG75800.026.0901491
CGCAGAG102200.025.984748
ACATGGG75700.024.7279013
TACATGG78300.024.6360382
GCAGAGT111100.023.4755489
TGGTATC20800.022.8424822
GAGTACT61000.022.27099412-13
TAAACCG1353.7902282E-421.1170835
GTGGTAT24400.020.8471361
CAGAGTA106800.020.39370210-11
AGAGTAC100150.019.99284610-11
GTACTTT68900.019.8544114-15
Produced by FastQC (version 0.11.5)